Objective To investigate the outcome of allergen skin prick test in children with bronchial asthma,and propose prevention strategy.Methods Sensitinogen prick test on the skin was applied on 200 children who were in asthma clinic of our hospital between Mar 2007 and Oct 2010.Then,the distribution of sensitinogen and their correlation with age and sex were analysed by SPSS 17.0.Results (1) The positive rate of sensitinogen prick test in 200 children with asthma was 72.5%(145/200).The positive rate of inhalant allergen and ingestion allergen were 62.5%(125/200),14.0%(28/200),respectively,of which dermatophagoides farinae,dermatophagoides pteronyssinus,outdoor mould,mugwort,cat epidermis,tree Ⅰ,egg,milk were 36.5%,40.5%,19.0%,17.0%,17.0%,13.0%,8.0%,5.5% respectively.(2) The frequency of sensitivity to cat epidermis,mugwort,outdoor mold and tree Ⅰ increased with age and the frequency of sensitivity to egg and milk decreases with age.The sensitivity rate to all other allergens had no relation with sex.(3) The severity of sensitivity to dermatophagoides farinae,dermatophagoides,ragweed and pigweed increased with age,while severity of sensitivity had no significant relation with sex among the 18 kinds of allergens.Conclusion Dermatophagoides farinae,dermatophagoides pteronyssinus,outside mould,mugwort,tree Ⅰ,cat epidermis,egg and milk are the major allergens in children with bronchial asthma,who should avoid contact with these facters.

Ischemic reperfusion is the common mechanism of some chronic diseases such as type 2 diabetes,coronary heart disease,hypertension,and so on.Previous studies of insulin resistance is mainly focused on the relationships of these chronics,while now,in recent years,the research between the insulin resistance and ischemic reperfusion has gradually become one of the new and hot points,closely related to the patient's prognosis.In this paper,we will review the insulin resistance and ischemic reperfusion according to the reported literature review.

Objective: To explore health resource allocation and optimization and evaluate the clinical departments efficiency in hospital. Methods: Taking a three level of first-class hospital in Beijing for example, the method of data envelopment analysis (DEA) was used for building a DEA model of clinical departments efficiency in hospital. Results: Based on the results of the DEA model, we drawn the technical efficiency and scale efficiency of the target clinical departments, obtained input redundancy rates and output deficiency rates of the non-effective decision-making units. Conclusion:By DEA evaluation results, the input redundancy and output deficiency of the target clinical departments are found. From the perspective of optimizing hospital resource allocation, measures to improve the hospital operational performance are proposed. There is important significance to improve the comprehensive competitiveness of the hospital.

Objective To compare the effects of fallopian tube recanalization and hydrotubation for treating partially obstructed fallopian tubes .Methods A retrospective analysis was conducted in 464 patients with partially obstructed bilateral fallopian tubes diagnosed by hysterosalpinography ,excluding other non -tubal infertility patients ,in the intervention department of our hospital Hospital from Jan .2005 to Dec .2010 .The patients were divided into 3 groups:fallopian tube recanalization group (157 cases) , hydrotubation group (155 cases) and control group(152 cases) .Results The intrauterine pregnancy rate of the fallopian tube reca-nalization group was higher than that of the hydrotubation group and control group .The ectopic pregnancy rate of the fallopian tube recanalization group was lower than that of the hydrotubation group and control group .The intrauterine pregnancy rate of the hydrotubation group was higher than that of the control group ,there was statistical difference between the two groups (P<0 .05) . While there was no statistical difference in the ectopic pregnancy rate between the hydrotubation group and the control group (P>0 .05) .Conclusion Active medical intervention can make the clinical benefit in the patients with partially obstructed fallopian tubes ,moreover ,fallopian tube recanalization is better than hydrotubation in the curative effect .

At present,in the opinions of the domestic and foreign scholars,the only hope of long-term survival even cure of hilar cholangiocarcinoma was obtained by radical resection(ie R0 resection).The application of preoperative assessment with appropriate and comprehensive strategies can judge the respectability and Intraoperative safety accurately to the tumor,thereby it can improve the radical resection rate and safety of patients with hilar cholangiocarcinoma.In this paper,preoperative assessment strategies are reviewed.

For the patients with early non-small cell lung cancer (NSCLC) who can tolerate surgery,surgery remains the standard treatment.For that patients who can not tolerate surgery,a large number of studies have shown that stereotactic body radiothery (SBRT) plays an indispensable role in the treatment of early NSCLC.

Objective Analysing the potential relationship of mismatch repair protein MLH3 gene C2531T mutation with oligospermia and azoospermia cases. Methods Using MLH3 C2531T as an example to establish a method of tetra-primer amplification refractory mutation system-PCR (4P-ARMS-PCR); We chose 81 cases of oligospermia, azoospermia patients and 80 normal controls, detecting MLH3 C2531T both using 4P-ARMS-PCR and PCR-RFLP; randomly to select 20% samples to sequence. Results The results suggested higher prevalence of CT + TT genotypes in patients as compared to the controls and association of T allele with disease (P <0. 01); PCR-RFLP results , 4P-ARMS-PCR results and sequence results are fully consistent. Conclusion 4P-ARMS-PCR can be a fast, easy-operating and accurate technology for detection of gene mutation of single nucleotide polymorphism (SNP) ; MLH3 C2531T polymorphism is related to oligospermia and azoospermia.

Objective To observe the effects of silencing IGF-1 receptor on the expression of Caspase12 , a endoplasmic reticulum specificity apoptosis protein of the peroxidation damage in L02 cells. Methods Four the recombined plasmids expressing short hairpin RNA (shRNA) specific for sileincing IGF-1R gene were constructed and these shRNAs were potentially targeted on different mRNA sequences of the IGF-1 receptor gene (Sh-H-IG1R- 1108, Sh-H-IGF1R-2797, Sh-H-IGF1R-3215 and Sh-H-IGF1R-3787). These constructs were classed as the experimental group. Another two constructs containing an irrespective sequence and no sequence were defined as a positive control group a negative control group , respectively. All these constructs were transfected into the L02 cells by Lipefectamine 2000. The interference effect was detected by RT-PCR after 24 h and the plasmid stably silencing the expression of IGF-1 receptor was selected for further analysis which was divided into four groups: the blank control group(Group A), the peroxidation damage group(Group B), the negative plasmid transfection group (Group C) and the positive plasmid transfection group (Group D). The expression of Caspase12 was detected by Westen Blot after 12 h. Results The amplification folds of experimental groups were statistically significantly lower than those of the Sh-H-IGF1R-V1 group and Sh-H-IGF1R-V2 group (P < 0.05). The silence effect of the Sh-H-IGF1R-3215 group (0.02 ± 0.01) and the Sh-H-IGF1R-3787 group (0.05 ± 0.05) were most stable and the repeatability of the later was better. The expressions of Caspase12 in Group B (2.41 ± 0.03), Group C (2.45 ± 0.02) and Group D (3.60 ± 0.06) were higher than Group A (0.34 ± 0.02) (P < 0.01). The Group Cexpression was higher than that of the Group B (P > 0.05). The Caspase12 expression in Group D was higher than that in Group B and C (P < 0.01). Conclusion (1) Of all the four constructs in this study, Sh-H-IGF1R-3787 is the most suitable for silencing IGF-1 receptor. (2) Silencing IGF 1R expression using shRNA plasmids can aggravate the L02 cells apoptosis, suggesting that the IGF-1/IGF 1R signal pathway might be involved in regulating the activation of apoptosis in L02 cells with peroxidation damag via endoplasmic reticulum stress.

Objective The aim of this paper is to develop a holistic mathematical model to quantify the correlation between skin thermal sensation and noxious stimuli,since skin thermal pain is one of the most common problems in everyday life and the understanding of the underlying physical mechanisms is still not clear.Methods The model is developed by considering the biothermomechanical behaviour of skin tissue and by incorporating the current understanding of biophysical and neurophysiological mechanisms of pain sensation.Results The proposed model consisted of three interconnected sub-models: peripheral modulation of noxious stimuli,which converts the energy from a noxious thermal stimulus into electrical energy via nerve impulses;transmission,which transports these neural signals from the site of transduction in the skin to the spinal cord and brain;and modulation and perception in the spinal cord and brain.The model predictions agree well with existing experimental data.Conclusion With this model,a direct relationship has been built between the level of thermal pain sensation and the character of noxious stimuli.

Objective:To examine relationship of postpartum depression with life events and social support. Methods: Thirty patients with postpartum depression and 32 normal controls were rated by a life events scale and a social support scale. Results: The number of negative life events were found to be higher in patients with postpartum depression as compared to normal controls. Social support of patients with postpartum depression was also found to be lower than their normal counterparts. Conclusion: Negative life events and deficient social support were significantly related to postpartum depression.