Objective To investigate the physical and mental health of Shandong medical personnel.Methods 3200 medical personnel were randomly selected from hospitals of Shandong province,selfhealth,depression and sense of work stress were measured and analyzed statistically.Results 36.3％ of medical staff was in depression state,and even about 22.5％ of the subjects was in moderate or severe depression state.Male and female medical staff showed no difference in sense of work pressure.Senior medical staff showed higher working pressure than middle-class,junior-class and low-level medical personnel,but middleclass,junior medical staff and low-level medical staff showed no significant difference.In the aspect of self-health status,male and female medical personnel showed no significant differences,senior,middle-class and junior-class medical staff reported worse self-health status than low-level medical personnel.Conclusions Managers should pay attention to physical and mental health of medical personnel,adopt pertinent measures to reduce their mental pressure in order to promote their physical and mental health.

Objective To determine whether platelet activation is associated with active ulcerative colitis (UC). Methods Platelet aggregability was assessed by means of SH 93 intelligent blood aggregation and coagulation tester; P selectin and thromboxane B 2 (TXB 2) were detected by means of ELISA method in patients with UC as well as in controls, including healthy volunteers and patients with irritable bowel syndrome (IBS). Colonoscopy and biopsy were performed in 43 patients with UC. Results Increased circulating platelet aggregability was detected in active ulcerative colitis compared with IBS and healthy controls ( P

The outbreaks of Zika virus infection in different regions of the world underscore the potential for the virus to spread further in the Americas and beyond.Zika is a mosquito-borneFlavivirusand is transmitted by Aedes spp. mosquitoes.It is has been reported recently that Zika virus intrauterine infection causes fetal brain abnormality and microcephaly, and WHO encourages pregnant women in countries at risk MUST protecting themselves from mosquito bites. Until now, there is no imported case in China.We have established resource and technology storage to reply to the potentialZika cases in the future, therefore, people do not need to panic in China.

Animal model plays an important role in prevention and control of infectious disease, which could link basic research in laboratory with clinical diagnosis and treatment for human patients.Mouse is the most widely used animal model of infectious disease, however, adult immunocompetent mice are resistant to some pathogens.The highly genetically diverse Collaborative Cross (CC) mice could recapitulate many of the genetic characteristics of an outbred population, such as humans.Based on this, this review will focus on the application and research progress of CC mice in infectious disease (including viruses, bacteria, fungi etc.), which could provide useful reference data for expansion of animal model resource bank, and implement of precision medicine of major and new emerging infectious diseases.We hope this review could serve as a modest spur to induce other researchers to come forward with their valuable contributions.

Objective To investigate the application of CUSUM (cumulative sum control chart) curve in cardiac clinical teaching of resident surgeon education.Methods We chose a surgeon of 10years work experience of attending the preparation operation of internal mammary artery as control reference (standard group,28 patients) and a resident engaged in professional clinical cardiac work no more than 5 years as group 1,a professional training physician engaged in cardiac surgery clinical work more than 5 years as group 2 and took 40 patients' data respectively from the two groups' preparation of internal mammary artery operation.And then we recorded the postoperative mammary artery flow,internal mammary artery preparation time and adverse events data and made statistics analysis with SPSS 19.0,and CUSUM curve with Matlab R2012b polynomial curve.Results When the density of operate interval and the operate numbers came to about 25 cases,the polynomial eurve's slope rate went up to 0,regardless the surgeons' experience,CUSUM value was accumulated from 66 to 76,and overcame the learning curve successfully.However,when the density of operate interval did no reach the standard,the surgeon's CUSUM ploynomial cmwe slope rate never reached to 0,and can't overcome the learning curve.Conclusion CUSUM curve gives a clear quantitative indicator to the cultivation of clinical physician,and makes teaching teachers can understand the young physicians' learning process,so as to change the teaching plan,help young physicians cross the learning curve as soon as possible.

Chimeric antigen receptor-transduced-T (CAR-T) cells shows strong cytotoxicity to the tumor with specific antigen by CTL effects and cytokines releasing without MHC-restriction.As multiple clinical trials from cancer centers around the West countries reported recent years,anti-CD19-CAR T cells can not only induce complete remission of B-cell malignancies resistant,but also eliminate the minimal residual disease (MRD) at the molecular level.It is believed that the obstacles from bench to clinic will be cleared and CAR will become one of the main cancer therapies with breakthroughs in comprehensive treatment of tumors.

T)of PTCH2 were identified and 9 polymorphisms (3 of which were novel) were determined in the present series. Conclusion: Although not as frequent as PTCH1 mutations, PTCH2 germline mutations were detectable in a subset of NBCCS patients with KCOTs. The pathogenetic role of these PTCH2 mutations is yet to be clarified.

This paper reviews the research progress in relations between indoor air pollution and human health for the last 20 years,and focuses on: the studies on environmental risk assessment methods; the major health effects caused by indoor air pollution; the minor and limited health effects caused by indoor air pollution; other key issues concerned.

Objective To assess accuracy and safety of ultrasonography guided or sonography X-ray guided percutaneous neddle biopsy(US-PCNB or CT-PCNB) for diagnosis of pulmonary masses.Methods Seventy one patients with radiographic evidences of pulmonary mass underwent US-PCNB or CT-PCNB.The diagnostic results of US-PCNB or CT-PCNB were confirmed by surgery or by follow-up examinations.Results The accuracy rate for diagnosis of pulmonary malignancy US-PCNB and CT-PCNB was 96 2% vs 94 7%.Complications occurred 3 87% vs 5 26%.Conclusions There was no statistically differences between US-PCNB and CT-PCNB.US-PCNB suitable for pulmonary tumors of around type.CT-PCNB suitable for pulmonary tumors of central type.

Objective To explore the role of high mobility group box protein 1 ( HMGB1 ) and receptor for ad-vanced glycation endproducts( RAGE) in lupus nephritis( LN) . Methods The serum and urine levels of HMGB1 were detected by ELISA in SLE patients and normal controls, and intrarenal expressions of HMGB1 and RAGE were detected by immunohistochemistry in renal tissues of SLE patients and normal-appearing renal tissues. Results
The serum and urine levels of HMGB1 were significantly higher in SLE patients compared to healthy controls( P<0. 01 ) , in patients with active disease compared to those with inactive disease ( P <0. 05 ) , serum levels of HMGB1 were found to be significantly higher in patients with renal involvement compared to those without renal in-volvement(P<0. 05). In addition, the levels of serum HMGB1 showed positive correlation with SLEDAI,urine pro-tein(24 h) and lencocyte count (P <0. 05). The expression of urine HMGB1 showed positive correlation with SLEDAI ( P<0. 05 ) . Intermediate-intensity staining of HMGB1 and RAGE was detected in renal tubules in normal-appearing renal tissues, however, both renal tubules and glomerular cells had the strong expression of HMGB1 and RAGE in SLE patients. The intrarenal production of HMGB1 and RAGE in SLE patients was obviously higher than that in normal-appearing renal tissues(P<0. 05), and the expression levels of HMGB1 in SLE IV and IV+V were higher than those in SLE II ( P<0. 05 ) . The expression levels of RAGE in SLE IV were higher than those in SLE II(P<0. 05). Conclusion HMGB1 and RAGE may play key roles in the pathogenesis of LN and may associate with the pathology category of LN.