Objective:To study the correlation of Th subsets in peripheral blood(PB)and IL-1 7 in gingival crevicular fluid(GCF)in the patients with periodontitis.Methods:1 0 healthy controls,1 1 cases with chronic periodontitis(CP)and 8 cases with aggressive pe-riodontitis(AgP)were included.Th1 ,Th2 and Th1 7 in PB were measured by flowcytometry,IL-1 7 in GCF was examined by ELISA. Periodental indexes(PLI,PD,AL and BI)were measured.Results:In the CP group and the AgP group,Th1 and Th1 7 were signifi-cantly higher,Th2 was lower than those in the control group(P <0.01 )(between CP and AgP groups,P >0.05),and IL-1 7 in GCF were significantly higher(P <0.01 )(between CP and AgP groups,P >0.05).In group CP IL-1 7 leve in GCF was positively correla-ted with PD and AL(P =0.000),in group AgP with PD(P =0.007).Conclusion:Deficiency of immunologic function and increase of IL-1 7 in the GCF is related with the onset and development of periodontitis.

Objective To explore the clinicopathological features,diagnosis and differential diagnosis of colonic muco-submucosal elongated polyp (CMSEP).Methods The clinical manifestations,endoscopic and histological features of three cases of CMSEP were analyzed.The literature was reviewed with regard to the diagnostic criteria and differential diagnosis.Results Three patients were male,and aged 25,45 and 75 years old.Two polyps were at transverse colon and one at rectum.Under colonoscope,all three cases showed the long pedunculated polyps.The surfaces of two polyps were smooth and the colors were similar to colonic mucosa while one polyp appeared mucosal erosion and congestion on the top.Under optical microscope,three cases had similar lesions.The axis of the lesions was expanded submucosa and the surface was the layer of colonic mucosa.The expanded and thick walled blood vessels were seen paralleled to the long axis in the submucosa of the axis of lesions.One polyp with mucosal erosion on the top presented with adenocarcinomal changes.Conclusions CMSEP is a kind of specific polypoid lesion,with characteristic colonoscopic and pathological features.Most are benign changes,however malignant changes occur in a few cases.In histology,CMSEP should be identified with mucosal prolapse syndrome associated inflammatory polyp.

Objective To investigate the influence of humanistic care on patients in the operation room.Methods 80 cases of surgery patients from November 2010 to October 2011 were given humanistic care,another 80 patients before implementation of humanistic care were chosen,and the satisfaction degree of doctors and patients and recovery of patients before and after the implementation of humanistic care were compared.Results After the implementation of humanistic care,the recovery of patients improved.The total satisfaction degree of patients and doctors reached 93.2％ and 98.1％,significantly higher than those before the impIementation,which were 82.1％ and 90.2％.Conclusions Implementation of humanistic care can improve the psychological and physical state of surgery patients,and help them gain better recovery.

Objective:To explore risk factors for slow coronary flow (SCF) phenomenon in male patients .Methods:A total of 123 male patients ,who were suspecting coronary heart disease because of chest pain ,then coronary angiography indica‐ted there was no significant lesion in epicardial coronary arteries ,were selected .According to presence of SCF or not ,they were divided into SCF group (n=69) and normal group (n=54) .Case data of two groups were retrospectively analyzed , and Logistic regression analysis was use to analyze risk factors for male SCF .Results:Compared with normal group ,there were significant rise in body mass index [BMI , (24. 01 ± 1. 57 ) kg/m2 vs . (27. 06 ± 3. 40 ) kg/m2 ] , hemoglobin level [ (145.79 ± 9.63) g/L vs .(150.00 ± 11.84) g/L] and hematocrit [ (0.43 ± 0.03) L/L vs .(0.45 ± 0.06) L/L] in SCF group ,P<0.05 all .Logistic regression analysis indicated that BMI (OR= 1.373 ,95% CI 1.146~1.645 ,P=0.001) and hemoglobin (OR=1.037 ,95% CI 1.000~1.176 ,P=0.048) were independent risk factors for male SCF .Conclusion:El‐evated body mass index and hemoglobin level are independent risk factors for slow coronary flow in male patients ,so corre‐sponding preventive measures must be carried out to improve prognosis .

AIM: To probe the quanlity and control of Suxiao Xiaoyan Gao. METHODS: The microscopy and TLC were applied to identify Scutellaria baicalensis; Hibiscus mutabilis; Curcuma longa; Rheum palmatum; Phellodendron chinese and borneol. The HPLC on C18column was used to determine the content of baicalin in Suxiao Xiaoyan Gao. Mobile phase was methanol∶0.4% phosphoric acid solution(47∶53). Detection wavelength was 280 nm. RESULTS： The micro-character and thin-layer chromatography was clear with no disturbance of negative sample. The average recovery of baicalin was (99±3)%, RSD was 3.35%. CONCLUSION: The method is simple and feasible. Its repetition is well. It can be used as a quality control method of Suxiao Xiaoyan Gao.

Objective:To observe the sedation effects of midazolam on maxillofacial postoperative patients with nasotracheal intubation. Methods:40 cases were equally and randomly divided into two groups: sufentanil group (group S) and sufentanil-midazolam group(group S-M).The continuous dose was 2 ml/h. If the patients feel uncomfortable, patient controlled intravenous analgesia (PCIA) was used. To observe the changes of circulation and respiration,the changes of visual analogue scale(VAS) and Ramsay score, the patients' bucking times within 24 h and the PCIA times, patients' total satisfactory degree and the complications during postoperative analgesia were recorded. Results:There were significant decreases in VAS of two groups after postoperative analgesia 1, 4, 12, 24, 48 hours(P

Objective To develop an improved enzymatic method for assaying creatinine in serum and urine. Methods Cratinine iminhydrolase catalyzes the conversion of creatinine to N-methylhydantoin and ammonia,the later combines with 2-oxoglutarate and NADPH in the presence of glutamate dehydrogenase.Before beginning the reaction sequence,endogenous ammonia is removed by an “ammonia scavenger system”that involves the same auxiliary reaction.The NADPH and 2-Oxoglutarate consumed are restored through a reaction catalyzed by isocitrate dehydrogenase.The activity of this strictly magnesium-dependent enzyme is completely blocked by magnesium-complexing reagent that acts promptly whenever the reaction has started with creatinine iminohydrolase as startor.The reaction is monitored kinetically by measuring the NADPH decrease via its absorbance at 340nm.Results The method affords a simple,rapid,and sensitive assay with good precision and extended linearity.Test results compare closely with the HPLC precedure.The proposed method is not subject to interference from several metablites or from the drugs used commonly in clinics.Conclusion The method is easily automated and is suitable for routine work in clinical laboratories.

Objective To evaluate video-assisted thoracoscopic sympathectomy(VTS) for the treatment of palmar hyperhidrosis(PH),and to analyze curative efficacies for surgeries on different sympathetic segments.Methods Medline search was done on PubMed and data of randomized controlled trials(RCTs) about comparisons of surgeries on different segments over the past decade were collected.According to the inclusion criterion,relevant articles were screened.Then we extracted data,assessed trail quality,and performed Meta-analysis by using RevMan 5.2 with postoperative compensatory hyperhidrosis(CH) as the main evaluation index.Results A total of 11 RCTs involving 1 413 patients were included,among which all patients underwent bilateral VTS.Ef cacious rates were similar between multiple and single ganglia sympathectomy(97.7％ vs 98.8％,P ＜0.01).However,single-ganglia group showed a lower risk of CH compared to multiple-ganglia group.Meta-analysis suggested that,in the subgroups of the single-ganglia VTS,no significant difference was found between T2/T3 and other segments in the risk of CH [T2 segment vs other segments,RR =1.11,95 ％ CI(0.99,1.24) ; T3 segment vs other segments,RR =0.99,95％ CI(0.89,1.11)].The risk of CH was significantly lower in T4 sympathectomy than in other segments [RR =0.67,95％ CI(0.57,0.79)].By comparison of T2,T3 and T4 sympathectomy,we found that low segment had a lower risk of CH than high segment[RR =0.75,95％ CI(0.68,0.84)].Conclusion Single-ganglia and low segment sympathectomy can significantly reduce the incidence of CH,and T4 is supposed to be the best segment for the treatment of PH.

Introduced in the paper are key measures and characteristics of the attending in charge system,following the theory of lean management and under leadership of the department head of the hospital.Authors hold the practice as enhancing administrative functionality of the department head, avoiding disadvantages incurred by traditional attending in charge system,and upholding quality of care management,making it a worthwhile attempt for China's healthcare system.

Objective To observe the clinical efficacy of Ziyin Yikang Recipe plus hexadecadrol on anti-HCG antibody positive secondary sterility and the influence on anti-HCG antibody titers. Methods Sixty patients with anti-HCG antibody positive secondary sterility were randomly divided into treatment group (TG) and control group (CG), 30 cases in each. Patients in the TG were treated with Ziyin Yikang Recipe plus hexadecadrol, while patients in the CG were given hexadecadrol only. The Chinese medicine symptom scores and changes of anti-HCG antibody titers were evaluated and compared between the two groups.Results The Chinese medicine symptom scores in the two groups decreased compared with those before treatment (P<0.05), and the TG showed a more significant decrease than the CG (P<0.05). The anti-HCG antibody titers in both groups dropped compared with those before treatment (P<0.05, P<0.01). There was no statistically difference between the two group after treatment (P>0.05). The total effective rate in the TG was higher that that in the CG (P<0.05), with 63.33% (19/30) in the TG and 47.66% (14/30) in the CG.Conclusion The treatment of Ziyin Yikang Recipe plus hexadecadrol on anti-HCG positive secondary sterility can obviously improve the clinical syndrome of yin deficiency and fire hyperactivity, and promote pregnancy success.