Objective To explore the factors of medical anthropology related to malaria control among Wa Ethnic minority in Ximeng County, Yunnan Province. Methods The study was conducted by the combination of anthropology and epidemiology, qualitative and quantitative, statistical analysis and description. Results Wa people considered malaria as one of three major diseases in the community. In Wa Ethnical language, the word of malaria, “Saihui” (Sai-illness and pain, Hui-being attacked quickly), does not mean of fever. About one third of Wa people had not thought fever as one of malaria symptoms. Most of Wa people only knew vivax malaria, but not falciparum malaria. Only 32.7% of people could connect malaria with mosquitoes, and a few of them could confirm mosquitoes as the only malaria vector. The proportions of sound knowledge of malaria causes, prevention and treatment seeking assessed were lower than 25.0%. As a result of multivariate logistic regression, 3 kinds of malaria knowledge were closely correlated each other, and the connection between causal knowledge and prevention (adjusted OR: 36.610, 95%CI: 10.242-130.866, P

Drug pair is the basic unit of compatibility of formulas.Through selecting the prescriptions of treating diseases of the liver in "Treatise on Cold Pathogenic Diseases" and "Synopsis of Golden Chamber",we,according to therapeutic methods,classify the commonly used drug pairs in treatment of hepatic disease of ZHANG Zhong-jing into eight kinds:regulating the flow of liver-qi,promoting blood circulation by removing blood stasis,dispersing pathogenic wind and calming liver wind,removing jaundice,clearing away liver-heat,expelling liver-cold and warming the liver,nourishing the liver,and harmonizing liver-spleen.And we try to find the compatibility law.

Drug resistance is major obstacles in the successful treatment of malignant solid tumors. Multiple mechanisms involeve in development of cancer drug resistance. Recent research suggests dysregulation of microRNAs is associated with cancer drug resistance. The profiles of microRNAs in drug resistance cancer cells or tissues are different with sensitivity cells or tissues in various solid tumors. Restoring microRNAs could improve chemosensitivity of cancer cells. MicroRNAs expression profiles may provide a critical link for understanding mechanisms involved in chemoresistance. We can also find a specific marker for screening chemosensitivity patients through identification of the microRNAs patterns of drug resistance cells or tissues.

Objective To explore the expression and significance of MTSS1 ( metastasis suppressor 1) in hepatocellular carcinoma.Methods MTSS1 expression was detected by immunohistochemistry in hepatocellular carcinoma, liver cirrhosis and normal liver tissues.Single-factor analysis was used to study the relationship with clinicopathological factor.Correlations between MTSS1 expression and TNM stage were analyzed with Spearman rank correlation analysis.Postoperative 5-year survival was evaluated using Kaplan-Meier survival curve analysis.Results The expression of MTSS1 in hepatocellular carcinoma was higher than normal liver tissue ( U = 168.000, P ＜ 0.05), and liver cirrhotic tissue ( U = 106.000, P ＜ 0.05); MTSS1 expression was correlated with TNM stage of hepatocellular carcinoma patients, lymph vascular invasion and tumor capsule ( separately U = 259.000, 258.500, 202.000, all P ＜ 0.05).MTSS1 expression in hepatocellular carcinoma was not correlated with patients age, gender, tumor size, AFP level, and hepatitis B surface antigen.MTSS1 expression and TNM stage of liver cancer patients was negatively correlated ( rs = - 0.383 , P ＜ 0.05 ).Postoperative 5-year survival of hepatocellular carcinoma patients with MTSS1 positive expression was significantly poorer than patients with negative and weakly positive expression (respectively 34.1% and 52.3% , x2 =6.386, P ＜ 0.05).Conclusions MTSS1 high expression may play an important role in the early hepatocellular carcinoma progression, indicating a poor prognosis.

Purpose　The aim is to study α-glucosidase inhibitor from Schisandra Chinensis(Turcz.)Baill.Methods　The inhibitor was purified by soaking,ultrafiltration,ion-exchange chromatography and Lead Acetate deposition.Results　A α-glucosidase inhibitor was obtained.Conclusion　The inhibitor was a kind of non-competition inhibitor with large molecular weight of over 5×105D.

[Objective]To sum up pro. Lian Jianwei’s clinical experience in treating acne. [Method] By proved recipe analysis, it shows pro. Lian Jianwei ’s treatment based on differentiation of signs from different angles and the clinical thought of flexibly applying ancient formulae on acne. [Result] Pro. Lian Jianwei is good at revising ancient formulae for treating acne with good effect, according to patients ’pulse signs and mechanism;if the food is stagnated in the gastric-intestine, which wil transform into heat and up along the channel, Baohe Pil shal be used to remove stagnation and heat; if the liver is de-pressed with heat, deficient blood and weak spleen, Xiaoyan Powder shal be used for regulation and stretching the wood; use Wenqing Drink to nourish blood and remove toxin for deficient blood and hot liver, toxic heat implicated in spleen and stomach; apply Zisheng Pil to regulate and tonify middle part of the body for deficient spleen and stomach with turbidness and dyspepsia, etc. [Conclusion] Pro. Lian Jianwei has rich clinical experience, his clinical thought of “treatment based on differentiation of signs, with flexible methods and formulae, coping with shifting diseases by sticking to a fundamental principle”, worthy our deep experiencing and study.

Objective To study the expression of IL 1?,TNF ? and nitric oxide (NO) in the cervical intervertebral disc and their roles in the pathogenesis of cervical spondylotic myelopathy (CSM). Methods Fifteen cervical discs were obtained from patients of CSM undergoing anterior disc surgery. Six cervical discs specimens were obtained in the surgical operation with patients with traumatic cervical injury served as the control. The specimens were analyzed for IL 1?、TNF ? by use of ELISA method, NO was measured by optical density assays. Results The contents of IL 1?,TNF ? and NO in disc of CSM patients increased significantly when compared with the control group. Conclusion The results of the present study indicated that IL 1?,TNF ? and NO may be involved in the pathogenesis of CSM.

Mitochondrial DNA mutations are one of the most important causes of sensorineural hearing loss. A1555G and C1494T mutations of mitochondrial 12S rRNA gene are the molecular basis for aminoglycoside hyper- sensitivity and can lead to aminoglycoside-induced hearing loss. Primary mutations in tRNA such as tRNA(Ser(UCN))7472insC are associated with syndromic hearing loss. While other mutations such as tRNA"(Se(UCN) )G7444A were considered synergy with the primary RNA mutations, modulating the phenotypic manifestation. This review de- scribes a detailed summary of hearing loss associated with mtDNA mutations and/or aminoglycoside antibiotics, and provides the possible molecular mechanisms in deafness expression.
Aminoglycosides ; adverse effects ; Anti-Bacterial Agents ; adverse effects ; DNA, Mitochondrial ; genetics ; Hearing Loss, Sensorineural ; chemically induced ; genetics ; Humans ; Mutation ; RNA, Ribosomal ; genetics

Objective The Application of Circular Mapping during Circumferential Pulmonary Vein Ablation (CPVA) for Atrial Fibrillation Guided by Ensite/NavX System. Methods To evaluate the effect of combined circular mapping on the success rate of CPVA. 61 consecutive cases (40 males and 21 females) were included in the study. 50 cases with paroxysmal atrial fibrillation (PAF) and 11 cases with chronic atrial fibrillation (CAF) underwent CPVA guided by the Ensite/NavX system. A circular electrode catheter was used to map the pulmonary vein (PV) potentials. Pulmonary vein isolation was taken as the endpoint for ablation. Results All the 61 cases underwent the procedure successfully. LPVs were isolated in 34 cases and 35 cases underwent single RPVs isolation. Circumferential ablation of both LPVs and RPVs were carried out in 23 cases. Atrial fibrillation was terminated when delivering radiofrequency energy in 16 cases with PAF and 5 cases with CAF. The termination sites were located near the anterior or posterior roof of the superior PVs in 19 cases and at the mid-portion of the posterior wall from RSPV in 1 case. Atrial fibrillation was terminated when after comple to LPV ablation in 1 case. During the follow-up of 6?2 months, 42 PAF cases and 5 of 11 CAF cases were atrial fibrillation free. The total success rate was 77%. Complications: Pericardium effusion was found in 1 case and disappeared after pericardium puncture and drainage. 1 case developed hemothorax and was cured after thorax puncture and drainage. Conclusion The application of circular mapping during circumferential pulmonary vein ablation guided by Ensite/NavX System contributes to improve the success rate of atrial fibrillation ablation.

This paper deals with the analysis of the phospholipids and fatty acids in Hippocomnpus kelloggi Jordan et Synder, H. Histrix Kaup, II. trimaculalus Leach, II. kuda Bleeder, II. japonicus Kaup, Solenognathus hardwickii(Gray), Syngnathoides biacufcatus(Bloch) and Tracliyrliamphus serralus (Temminck &. Schlegel).The total phospholipids were extracted with Folch solvent and determined by molybdate blue spec-trophotometric method. The result showed that the contents of total phospholipids in these spccimins were 2. 56-7. 82mg/g.The nine kinds of phospholipid standards of absorbance proportional coefficient were detected in this paper. The phospholipid components were separated and determined by using thin - layer chro-matographic method and Vaskovsky solvent as a specific spray reagent. The quantitative analysis of the distribution of various phospholipids components in these specimen ts were carried out, by using TLC scanning and the corrective method of absorbance proportional coefficient. The resut showed that the Hippocampus contains about five phospholipids: lysophosphatidylcholine, sphingornyelin, phophatidylcholine, phosphatidylinositol, and phosphatidylethanolamine. The phosphatidylserine , phosphatidylglycerol, diphosphatidylglycerol and phosphatidic acid were more in Syugnathus than in Hippocampus, But in all these specimens, the main phospholipid components were lysophos- phatidylcholine, sphingornyelin and phosphatidylcholine.Fourteen fatty acid compositions in these specimens were analysed by GC -MS -DS. The result showed that the main fatty acid were hexadecanoic acid, 9 - octadecenoic acid, 8,11- octadeca-dienoic acid and 1,7,10,13,16,19 - docosahexaenoic acid.