Scientific research fund is an important component of scientific research management,and an important precondition for scientific research activity. This paper explores the existing problems in the scientific research fund management of medical university, and suggests that only standardization of the fund management can guarantee the efficiency the fund use, and realize the scientificity, normalization, rationality of the research fund management, which will all contribute the improvement of research quality.

Objective To develop a convenient and effective molecular authentication method for identifying Dendrobium nobile from other species of Dendrobium Sw.Methods Based on rDNA ITS sequences of D.nobile and other species of Dendrobium Sw.of Huangcaos and Fengdous,unusual sequence sites were confirmed and an allele-specific diagnostic primer pair was designed for D.nobile. Diagnostic PCR was performed using the primers with the total DNA of D.nobile and other species of Dendrobium Sw.as templates to establish the positive PCR condition for D.nobile.Results An about 300 bp DNA positive fragment was amplified from D.nobile with anneal temperature at 66 ℃ and anneal time at 40 s,whereas no any DNA fragment was amplified from the rest 39 species of Dendrobium Sw.Conclusion D.nobile could be effectively distinguished from other species of Dendrobium Sw.by diagnostic PCR with allele-specific diagnostic PCR,which is not only effective and specific,but also simple and timesaving,and has a comprehensive application prospects.

At present, too much importance has been attached to avian influenza globally. The reasons for this and the possibility of contagion from human to human were discussed . As to prevention and control of avian influenza, suggestions were made to find early human cases in local hospitals and local CDC (Center for Disease Control and Prevention), to make the laboratory staff follow the bio-safety principle for diagnosis of avian influenza.

Objectives:To observe the effects of enteral nutrition on cell immune in patients with acute renal failure. Methods:The changes of cell immune were compared in 22 patients with acute renal failure before and after EN treatment. Results:There were significant improvements in cell immune after 10 days of EN. CD3,CD4,CD4/CD8 and NK acitivity( P

OBJECTIVE:To provide the reference for the set-up of precautions against flu pandemic in China.METHO_DS:RNA of influenza viruses isolated from flu children in flu seasons between 2003 and 2005 in Beijing was extracted and amplification of M2 gene fragments associated with ion channel were performed by one step reverse transcription–polymerase chain reaction(RT-PCR).The sequencing of amino acid was determined,and its bioinformation was analyzed with software,then the positions of amino acid for resistance to amantadine were detected.RESULTS:20 of 31 isolated strains were resistant to amantadine,the percentage of which was 66.7%.CONCLUSION:The monitoring of drug resistance to flu viruses should be strengthened in China.

Objective:To explore the relations between freshmen's self-identity status,personality characteristics and mental health status.Methods:188 new students were asked to complete the questionnaires of mental health scale and personality scale from the China college students mental health evaluation system,and eollnge students self- identity status scale.Results:①the most status of identity is achievement(43.1%),then the identity diffusion (30.9%);②male students in compare with female,were more likely in achievement status(60.7?13.8/53.9?11.4,P0.406);③the achieve- ment status had obvious negative correlation with depression,dependency(r=-0.17～-0.24),the diffusion status had obvious positive correlation with depression,social-withdrawal,and impulsion(r=0.15～0.34);④active,ac- commodating,tough-minded personality characteristics had obviously negative correlation with mental symptoms(r= -0.16～-0.56).Conclusions:encourage college students to form the achievement ego-identity status,to be ac- tive,accommodating and tough-minded,will be helpful for their psychological health.

AIM: To evaluate and analyze the morphological property and physiological activity of cartilaginous loose bodies in the knee joint, to research the changeable mechanism of histological morphous and physiological function. METHODS: The experiment was conducted at the Department of Pathology of the First Affiliated Hospital of Anhui Medical University, the Physical Testing & Chemical Analysis Center of Anhui Medical University, and the Electron Microscope Center of Anhui Provincial Hospital Affiliated to Anhui Medical University from January 2005 to January 2006. ①Experimental samples were 30 cartilaginous loose bodies all through arthroscopy as experimental group in which Group A (less than 6 months) had 14 samples and group B (between 6 and 12 months) 10, group C (more than 12 months) 6. Another 30 control samples were originated from non-weight loading side of articular cartilage through special equipment by arthroscopy. ②All samples were carried out the morphological measurement, light and transmission electron microscope exam, Ⅱ-collagen immunohistochemical stain, the measurement of collagen, calcium and fosforus. RESULTS: ①To compare the samples in the group A and control group, they had the identical or similar morphosis, microscopic structure of chondrocyte and distribution of extracellular matrix, the statistical treatments of the content of calcium, fosforus and collagen showed that the differences of scores between two groups were not statistically significant [calcium:(3.120?0.224),(3.090?0.231) mg/g;fosforus:(0.432?0.045),(0.455?0.067) mg/g;collagen:(462.43?17.12)(466.71?14.59) mg/g]. General speaking, comparing with the control articular cartilage, the loose bodies in group A had the normal histological property and physiological activity. ②Compared with control group, the samples in group B and C had the tendency of fibrous degeneration: necrotic or degenerative chondrocytes increasing, the contents of proteoglycan and Ⅱ-collagen decreasing, the tendency in group C more than group B, but the cartilaginous loose bodies in group B and C had many active chondrocytes mainly. Total content of collagen [(441.20?16.49),(417.33?8.38) mg/g] and Ⅱ-collagen decreased, while the contents of calcium and fosforus increased [calcium: (3.696?0.442),( 6.469?0.555) mg/g;fosforus:(0.523?0.068),(0.683?0.056) mg/g]. Generally, compared with the control articular cartilage, the histological property and physiological activity of the loose bodies in groups B and C had changed. CONCLUSION: The cartilaginous loose bodies can remain its histological property and physiological activity in the knee articular cavity for a long time, but it has the tendency of disorganization and physiological activity descending as time prolongs.

Chylous Ascites ; congenital ; therapy ; Humans ; Infant ; Male ; Parenteral Nutrition, Total ; Somatostatin ; therapeutic use

Objective To explore the feasibility of establishing a modified mouse model of pressure overload induced left ventricular hypertrophy by transverse aortic constriction(TAC).Methods 55 C57BL/6 female mice were randomly divided into three groups:severe TAC group (n =27),moderate TAC group (n =7) and sham surgery group (n =21),respectively.By ligating the aorta arch between innominate artery and the left common carotid artery with modified techniques in a minimally invasive way,moderate or severe aortic constriction were established successfully and reliably to mimic left ventricular(LV)outflow obstruction; to correctly evaluate the cardiac structural and functional responses to aortic arch banding,2-dimention (2D) and M mode transthoracic echocardiography (TTE) were deployed to monitor the LV contractile function and assess the LV hypertrophic changes induced by pressure overload at 4 weeks after the surgery.Results The mouse model of aortic constriction was established successfully with a post-operative survival rate more than 88％.And all these operated mice were able to survive at least 4 weeks long.Eccentric left ventricular hypertrophic changes were detected with echocardiographic measurement 4 weeks after the banding operation in both mTAC and sTAC group,as dilated left ventricular lumen with enlarged LV end-diastolic dimension (LVEDD) and LV end systolic-dimension (LVESD) were confirmed.Mice with moderate banded aorta exhibited a compensated LV hypertrophy with preserved contractile functions and satisfactory ventricular wall movements to some extent,although left ventricular fractional shorting (LVFS) reduced gradually from 0.403 ± 0.007 to 0.340 ± 0.015 (P＜0.05) ; while in severe banded (sTAC) mice,LVFS reduced more significantly as a sharp decrease from 0.438 ± 0.011 to 0.216 ± 0.012 (P ＜ 0.01) were detected,combined with poor contractile function and stiff ventricular wall movements,exhibiting a de-compensated pathological left ventricular hypertrophy.Conclusion This modified TAC opcration could be easily carried out,and the TAC mouse model mentioned in the present research was an effective pressure overload model with several superiorities including less trauma,improved post-operative survival rate,rapid recovery and satisfactory reproducibility,thus a better and recommended mouse model for specific research purpose concerning LV hypertrophy mechanistic studies.

Apolipoprotein E (ApoE) has gene polymorphism (ε2,ε3,and ε4).It has been confirmed that ApoE structural and functional abnormlities are closely associated with the hyperlipidemia,formation of atherosclerosis,and its severity,while both hyperlipidemia and atherosclerosis are the risk factors for ischemic stroke.In recent years,although the relationship between the ApoE gene polymorphism and ischemic stroke has been extensively studied,the conclusions are not consistent.This article reviews the correlation studies between the ApoE gene polymorphism and ischernic stroke.