Objective　To evaluate the invasive ability of bladder transitional carcinoma cells(TCC),with a new method.Methods　MTT colorimetric assay combining with Boyden chamber system was used to measure the invasive ability of three bladder TCC lines EJ、T24、BIU-87. Invasive Index=experimental absorbance/control absorbance×100%.Tumor cells mortility growth curve and coloney forming test were measured for these 3 lines.Results　The Invasive Index of three cell lines were EJ 0.326%±0.046%，T24 0.569%±0.044% and BIU-871.256%±0.066%. The Motility Rate were EJ 0.508%±0.037%，T24 0.672%±0.039% and BIU-871.524%±0.043%.Conclusion　This method can evaluate the invasive ability of tumor cells accurately and it is sensitive,safe and rapid. It also can be done easily and has no radiation pollution.

Pit cells (PCs) are hepatic natural killer cells(HNKCs), and play an important role in resisting tumor, resisting virus, regulating hepatocyte growth and differentiation, inhibiting liver fibrosis and so on. In the paper, research progress in liver pit cells is briefly summarized.

Objective To study the effect of nifedipi ne ,an endothelin antagonist,in acute renal function impairment caused by ESWL. Methods 60 cases of renal stone underwent anaesthesia-free ESWL were radomely assigned into 2 groups,the nifedipine group and the control (n=30 each).In the former group,nifedipine was given 3 days before ESWL.Plas ma endothelin,urinary ? 2-microglobulin,and urinary Tamm-Horsfall protein we re assessed to evaluate the renal function. Results In t he controls,it was a significant increase of plasma endothelin and urinary ? 2-MG(P

Purpose:To improve the knowledge of primary extragonadal seminoma.Methods:Five cases proved by histopathology were reported in this article, every patient received surgical operation plus subsequent chemotherapy or radiotherapy . Results:all of them are alive now. the histogenesis, clinical features, principle of treatment of primary extragonadal seminoma are discussed with review of literature.Conclusions:Primary extragonadal seminoma is rare, histopathology is very important for diagnosis and selection of therapy.

Objective To study the effect of Fas gene transfection on bladder cancer cells. Methods Fas cDNA were transduced into bladder cancer cells EJ by DOTAP liposomal transfection regeant. Northern blot,in situ hybrization and flow cytometric evaluation were used to confirm the Fas mRNA and protein expression. The apoptosis and proliferation of EJ cells pre and posttransfection induced by cisplatin were analysed by flow cytometry、DNA ladder and MTT methods. Results Transfection of Fas gene can significantly upregulate the expression of Fas in human bladder cancer EJ cells. Apoptosis and decrease of proliferation were easily induced by cisplatin in the transfected EJ cells. Conclusions Fas system might involve in the development and progression of urogenital malignant tumors. Transfection of Fas gene by lipofectin can significantly upregulate the Fas expression in target cell. The synergistic cytotoxic effect obtainted in EJ cells suggested that combined use of Fas gene transfection and cisplatin may help in the treatment of cisplatin resistant bladder cancer.

Objective To study the diagnosis and trea tm ent of multiple endocrine neoplasia type 2b. Methods On e case of MEN2b was reported.The clinical features,the diagnosis and treatment o f MEN2b were reviewed and presented. Results The diagno sis was established on B-ultrasonography and CT scanning,and the patient was cu red by operation. Conclusions MEN2b has been rare.Endoc rine check up,imaging procedures,family investigation and DNA analysis are very important to early diagnosis.Surgical removal of the tumors is effective.

Objective To study the correlation between GSTM1 genetic polymorphism and bladder cancer susceptibility. Methods In a case control study, the GSTM1 genotype was assessed by a PCR based method. 69 patients with transitional cell carcinoma of the bladder and 88 controls matched for age and sex were studied. Results The frequency of GSTM1 null genotype among the bladder cancer patients was 58% compared with 41% among controls(OR=2.0, 95%CI=1.05～ 3.79 ,? 2=4.51, P

Objective To explore the causes and treatment of difficulty of urination after suprapubic prostatectomy. Methods 56 cases with difficulty of urination after suprapubic prostatectomy were reviewed.The causes of difficulty of urination were bladder outlet stricture,urethral stricture,relapse of BPH and other nonsurgical causes. Results 48 cases were surgically managed,and the success rate was 94%.The follow up time was 2～84 months with a mean of 38 months,all of them urinate normally except in 3 suprapubic cystostomy has been mandatory. Conclusions Correct presurgical diagnosis,adequate surgical treatment and postsurgical management are key points in preventing postsurgical difficulty of urination.

Purpose: To investigate the effect of HSV-TK/GCV system on human prostate cancer. Methods: MTTassay, flow cytometry( FCM), optical and electron microscopy were used to determine the sensitivity of GCV on prostate cancer cell( PC-3m) after being tranfected with HSV-TK gene. Results: It was found that GCV had significant cytotoxic activity on HSV-TK gene-transduced prostate cancer cells, but little effect on the non-transduced cells. Conclusions: The experiment indicates that HSV-TK/GCV system has significant antitumor activities on the TK gene-transduced prostate cancer cells in vitro.

Objective To develop a stable and quick method to determine the urea in swimming pool water.Methods On the conditions of strong acid and calefaction,urea can react on antipyrine and diacetyl monoxime and become yellow which can be determined by colorimetric analysis in 450 nm with the ALLIANCE FUTURA continuous flow injection auto analysis system.Results Determined by this method,the linear range was 0.5-4.0 mg/L,the regression equation was y=0.077 5x+0.021 9,the correlative coefficient r=0.999,the detection limit was 0.07 mg/L.With residual chlorine of 0.3 and 3.0 mg/L,the recovery rates were 98.8%-104.0% and did not disturb the determination.The results determined by the present method were the same as those by the method in GB/T 18204.29-2000(Wilcoxon Signed Ranks Test:Z=0.051,P=0.959).Conclusion Determination of the urea in swimming pool water with continuous flow injection auto analysis,the sensitivity,accuracy and limit of determination can meet to the requirement,moreover,the analysis speed is faster,less reagent will be needed,the present method can be generalized in the laboratory that has the conditions.