Pit cells (PCs) are hepatic natural killer cells(HNKCs), and play an important role in resisting tumor, resisting virus, regulating hepatocyte growth and differentiation, inhibiting liver fibrosis and so on. In the paper, research progress in liver pit cells is briefly summarized.

Objective　To evaluate the invasive ability of bladder transitional carcinoma cells(TCC),with a new method.Methods　MTT colorimetric assay combining with Boyden chamber system was used to measure the invasive ability of three bladder TCC lines EJ、T24、BIU-87. Invasive Index=experimental absorbance/control absorbance×100%.Tumor cells mortility growth curve and coloney forming test were measured for these 3 lines.Results　The Invasive Index of three cell lines were EJ 0.326%±0.046%，T24 0.569%±0.044% and BIU-871.256%±0.066%. The Motility Rate were EJ 0.508%±0.037%，T24 0.672%±0.039% and BIU-871.524%±0.043%.Conclusion　This method can evaluate the invasive ability of tumor cells accurately and it is sensitive,safe and rapid. It also can be done easily and has no radiation pollution.

Objective To study the effect of nifedipi ne ,an endothelin antagonist,in acute renal function impairment caused by ESWL. Methods 60 cases of renal stone underwent anaesthesia-free ESWL were radomely assigned into 2 groups,the nifedipine group and the control (n=30 each).In the former group,nifedipine was given 3 days before ESWL.Plas ma endothelin,urinary ? 2-microglobulin,and urinary Tamm-Horsfall protein we re assessed to evaluate the renal function. Results In t he controls,it was a significant increase of plasma endothelin and urinary ? 2-MG(P

Objective To report our experience on construction of detenial sigmoid neobladder after laparoscopic radical cystectomy in 71 cases.Methods From August 2002 to May 2006,a total of 71 patients with invasive bladder carcinoma underwent construction of detenial sigmoid neobladder after laparoscopic radical cystectomy in our hospital.After the bladder was excised by laparoscopy,a 5-to 7-cm incision was made on the abdomen to remove the resected tissues,and then a 15-to 20-cm sigmoid colon was resected,the two colic bands opposite to the mesentery and the circular muscle and seromuscular layers between them were removed to construct a detenial sigmoid neobladder.Afterwards,the neobladder was anastomosed with the posterior urethra.Laparoscopic anastomosis was performed in 26 of the cases.Results The operation time was 240-390 min totally in the 71 cases.Laparoscopic radical cystectomy was finished in 80-270 min(mean,180 min),and the open surgery was completed in 160-240 min(mean 140 min).Oral intake was started at day 4-8 postoperation,ureteral stents were removed at week 3-4,and urethral catheter was removed at week 4.Three months after the operation,the mean intra-bladder pressure was 22.0 cm H2O(17-38 cm H2O),average bladder reservoir was 340 ml(200-410 ml),volume of residual urine was less than 25 ml in all the cases(

Objective To study the effect of Fas gene transfection on bladder cancer cells. Methods Fas cDNA were transduced into bladder cancer cells EJ by DOTAP liposomal transfection regeant. Northern blot,in situ hybrization and flow cytometric evaluation were used to confirm the Fas mRNA and protein expression. The apoptosis and proliferation of EJ cells pre and posttransfection induced by cisplatin were analysed by flow cytometry、DNA ladder and MTT methods. Results Transfection of Fas gene can significantly upregulate the expression of Fas in human bladder cancer EJ cells. Apoptosis and decrease of proliferation were easily induced by cisplatin in the transfected EJ cells. Conclusions Fas system might involve in the development and progression of urogenital malignant tumors. Transfection of Fas gene by lipofectin can significantly upregulate the Fas expression in target cell. The synergistic cytotoxic effect obtainted in EJ cells suggested that combined use of Fas gene transfection and cisplatin may help in the treatment of cisplatin resistant bladder cancer.

Objective To study the diagnosis and trea tm ent of multiple endocrine neoplasia type 2b. Methods On e case of MEN2b was reported.The clinical features,the diagnosis and treatment o f MEN2b were reviewed and presented. Results The diagno sis was established on B-ultrasonography and CT scanning,and the patient was cu red by operation. Conclusions MEN2b has been rare.Endoc rine check up,imaging procedures,family investigation and DNA analysis are very important to early diagnosis.Surgical removal of the tumors is effective.

Objective To study the correlation between GSTM1 genetic polymorphism and bladder cancer susceptibility. Methods In a case control study, the GSTM1 genotype was assessed by a PCR based method. 69 patients with transitional cell carcinoma of the bladder and 88 controls matched for age and sex were studied. Results The frequency of GSTM1 null genotype among the bladder cancer patients was 58% compared with 41% among controls(OR=2.0, 95%CI=1.05～ 3.79 ,? 2=4.51, P

Objective To explore the causes and treatment of difficulty of urination after suprapubic prostatectomy. Methods 56 cases with difficulty of urination after suprapubic prostatectomy were reviewed.The causes of difficulty of urination were bladder outlet stricture,urethral stricture,relapse of BPH and other nonsurgical causes. Results 48 cases were surgically managed,and the success rate was 94%.The follow up time was 2～84 months with a mean of 38 months,all of them urinate normally except in 3 suprapubic cystostomy has been mandatory. Conclusions Correct presurgical diagnosis,adequate surgical treatment and postsurgical management are key points in preventing postsurgical difficulty of urination.

Purpose: To investigate the effect of HSV-TK/GCV system on human prostate cancer. Methods: MTTassay, flow cytometry( FCM), optical and electron microscopy were used to determine the sensitivity of GCV on prostate cancer cell( PC-3m) after being tranfected with HSV-TK gene. Results: It was found that GCV had significant cytotoxic activity on HSV-TK gene-transduced prostate cancer cells, but little effect on the non-transduced cells. Conclusions: The experiment indicates that HSV-TK/GCV system has significant antitumor activities on the TK gene-transduced prostate cancer cells in vitro.

Purpose:To improve the knowledge of primary extragonadal seminoma.Methods:Five cases proved by histopathology were reported in this article, every patient received surgical operation plus subsequent chemotherapy or radiotherapy . Results:all of them are alive now. the histogenesis, clinical features, principle of treatment of primary extragonadal seminoma are discussed with review of literature.Conclusions:Primary extragonadal seminoma is rare, histopathology is very important for diagnosis and selection of therapy.