Objective To analyze the surgical treatment choice of hepatolithiasis and its curative effect. Methods The clinical data of 120 patients with hepatolithiasis admitted in our hospital were randomly selected and retrospectively analyzed. 43 cases were undergone cho-ledocholithotomy, 37 cases were given merotomy of live, 31 cases were given hepatic duct plasty and choledochojejunostomy, and 9 cases were performed fiber choledochoscope lithotomy. Results All the 120 cases were followed-up from 1 year to 10 years. The postoperative re-sidual stone rate, good rate, and occurrence rate of complications were respectively:42. 23%, 78. 69%, 9. 23% in group of choledocho-lithotomy;8. 05%, 95. 93%, 18. 09% in group of merotomy of live;13. 33%, 87. 33%, 21. 32% in group of hepatic duct plasty and cho-ledochojejunostomy;31. 98%, 78. 04%, 15. 69% in group of fiber choledochoscope lithotomy. The total residual stone rate was 24. 17%, the total good rate was 85. 83%, and the occurrence rate of complications was 15. 83%. Conclusion Choose the suitable operation method according to medical history of patients is the the key to reduce the residual stone rate and the occurrence rate of complications, thus elevating therapeutic efficacy of hepatolithiasis.

0.05). CONCLUSION:3M Ketac Molar ultrahard glass ionomer cement is a good substitute for silver amalgam as the filling material for dental cavities,for not only can it repair dental facial defects,but also make teeth good-looking and prevent dental caries.

Objective To observe effects of soaking therapy with traditional Chinese medicine on athlete's foot. Methods346 cases of the treatment group (175 cases of erosive type and 171 cases of bullous type) were treated with soaking therapy by traditional Chinese medicine and 335 cases of the control group (170 cases of erosive type and 165 cases of bullous type) were treated with 3% Clotrimazolum ointment. Effects of two groups were compared.Results Effect of the treatment group was obviously more superior to that of the control group (P<0.0001). ConclusionsIt shows easy and effective that patients with athlete's foot were treated by soaking therapy with traditional Chinese medicine.

The professionalism view of qualified person and some reasonable proposals for the promotion of the professionalism are put forward. The necessity of the professionalization of qualified person and the effective measures of perfecting the professionalization are studied by the comparative analysis between home and abroad combined with the game model analysis. Under the current national conditions, qualified person are repositioned, and after learning the advanced experience of the EU, the registration system is strictly carried out, which can promote the professionalism of qualified person and make relevant measures in order to restrict the behavior of qualified person.

Objective To explore whether ganoderma spores could help the neural epithelial cell of embryonic neural tube stagnated by retinoic acid in G-0/G-1 phase reenter the cell cycle,keep on proliferating and differentiating,and could reduce the occurrence of neural tube defects(NTDs). Methods When E7.75d,mice of the control group and the ganoderma spores group were given intragastrically onetime retinoic acid all-trans.Then the ganoderma spores solution was given intragastrically to the ganoderma spores group.At E10.5d,The embryos of the two groups were taken out and the ratio of NTDs was counted respectively Immunofluorescence histochemistry and flow cytometry were applied to examine the expression of nestin,and DNA quantity staining and RT-PCR to detect the mRNA transcriptions of Cdk2 and Cdk4. Results The radio of NTDs in the ganoderma spores group was lower than that in the control group remarkably,but the level of nestin was higher.With the neural tube cell cycles compared the cell radio of G-0/G-1 phase in the control group was higher than that in the ganoderma spores group,while the ratio in the S phase in the control group,it was lower than that of the normal embryos in the ganoderma spores group.The neural epithelial cell of embryonic neural tube in the ganoderma spores group could transcript Cdk4 mRNA normally,with a low transcription rate in the control group.Conclusion Ganoderma spores can reduce the occurrence of embryonic NTDs induced by retinoic acid in pregnant mice.

This paper analyzed the cultivation of postgraduates of anesthesiology and the academic achievements in anesthesiology department from 2003 to 2010 in our hospital and explored the experiences in postgraduate cultivation.Cultivation of postgraduates of anesthesiology should focus on multidisciplinary integration,cross-discipline communication,extension of scientific thinking,enhancement of clinical skills so as to create the opportunity for clinical and research work.

Objective Brain -derived neurophic factor ( BDNF) has played an important role in the repair of cauda equina Injury.The aim of this article was to observe the influences of Miao medicine Sheng Xian Decoction on structural change and BDNF protein expression in the sacral spinal cord of rats with cauda equina injury . Methods The rat model of cauda equina injury was es-tablished by using nerve clamping .Rats were randomly divided into the following groups:normal group , control group , Miao medicine group and mecobalamin group , 15 rats in each group .According to the death time after modeling , the rats in each group were subdivi-ded into 1d group, 7d group and 14d group, 5 in each subgroup.The nerve function recovery of rats in every group was observed through Basso , Beattie & Bresnahan locomotor rating score ( BBB score ) .The structural change of sacral spinal cord was observed(11.40 ±1.14) and the mecobalamin group (11.40 ±0.89) (P>0.05).On the 7th, 14th day after modeling, the scores of the Miao medicine group (17.0 ±1.00, 19.60 ±0.55) and the mecobalamin group (16.2 ±0.84, 18.80 ±0.84) were much higher than the control group (14.00 ±0.70, 16.40 ±0.55) (P<0.05).There was no significant difference between the Miao medicine group and the mecobalamin group (P>0.05).The scores of the control group , the Miao medicine group and the mecobalamin group showed an increasing tendency with time, and the difference of scores among the groups are of statistical significance (P<0.05).HE staining:Compared with the normal group , the spinal cord structure of the control group was damaged and glial cell proliferation increased signif -icantly.Compared with the control group , rats in the Miao medicine group and the mecobalamin group were lightly damaged in spinal cord and got improvement in the glial cell proliferation as well as an increase in the number of normal neurons .Immunohistochemistry staining:On the 1st day after modeling, the MOD values of the control group (0.152 ±0.007) , the Miao medicine group (0.156 ± 0.008) and the mecobalamin group (0.162 ±0.008) were much higher than that of the normal group (0.130 ±0.006), and the difference was of significant difference (P<0.05).Moreover,the MOD value of the mecobalamin group was much higher than those of the control group and the Miao medicine group , which was of significant difference (P<0.05); there was no statistical significance between the control group and the Miao medicine group (P>0.05).On the 7th and 14th day after modeling, the MOD values of the Miao medicine group (0.205 ±0.009, 0.183 ±0.008) and the mecobalamin group (0.217 ±0.033, 0.187 ±0.007) were much higher than that of the control group (0.181 ±0.007, 0.161 ±0.014), which was of significant difference (P<0.05).There was no statistical significance between the values of Miao medicine group and the mecobalamin group (P>0.05).The MOD values of the control group , the Miao medicine group and the mecobalamin group showed a tendency of increase followed by decrease with time .The MOD values of 3 subgroups in the Miao medicine group and the mecobalamin group had no statistical significance (P>0.05).The MOD value of 14d subgroup was much lower than 7d subgroup in the control group (P<0.05), however, compared with 1d subgroup in the control group, there was no significant difference (P>0.05).There was no significant difference among the MOD value of 3 subgroups in the normal group (0.130 ±0.006, 0.130 ±0.006, 0.130 ±0.008) (P>0.05). Conclusion Miao medicine Sheng Xian Decoction can promote neuron survival and improve nerve functions through increasing the BDNF expression in the sacral spinal cord of rats with cauda equina injury .

Integrin is a kind of crucial adhesive molecule on the membrane,and it exerts its function through activating its intra-cellular effectors.One of the most important effectors is integrin-linked kinase (ILK),which is a serine/threonine protein kinase located in focal adhesions and has a key position in transducing many of the biochemical signals.It plays an important roles in regulating fundamental processes such as cell-matrix interactions,growth,proliferation,survival,differentiation,migration,invasion and angiogenesis under both physilogical and pathological processes.

Primary biliary cirrhosis (PBC) is an autoimmune disease with unknown causes. Most PBC patients have abnormal lipid metabolism characterized by hypercholesterolemia. Paradoxically, clinical observations and pre-experimental studies showed that the risk of hyperlipidemia associated cardiovascular event and the mortality of PBC patients were not increased. In this review we summarize the possible reasons and the underlying mechanisms.

Objective:To explore the role of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1) in oxidized lowdensity lipoprotein (ox-LDL)-induced atherosclerotic inflammation. Methods: Ox-LDL was prepared by oxidization of native LDL ;different concentrations of ox-LDL were used to treat U937 cells for 48 h. Cells and supernatants were collected. The expression of Siglec-1 protein and mRNA was examined by flow cytometry (FCM) and real-time quantitative RT-PCR, respectively. The levels of MIP-1α, MCP-I and IL-8 in the supernatants were determined by ELISA. Results: Stimulation with ox-LDL significantly increased Siglec-1 protein and mRNA in U937 cells compared with the control group ( P < 0. 01). Meanwhile, the levels of MIP-Iα, MCP-I and IL-8 in the supernatants were also significantly increased in a concentration-dependent manner compared with those in the control group(P<0. 01). Conclusion: The proatherogenic effect of ox-LDL may be partly through up-regulating Siglec-1 expression in macrophages and enhancing the expression of inflammatory cytokines and chemokines. The activated macrophages recruit more macrophages and lymphocytes to the site of atherosclerotic plaques and exacerbate the inflammatory responses.