Objective To compare the clinical efficacy of breast-conserving surgery and modified radical mastectomy for early breast cancer and their influence on quality of life.Methods 78 patients with early stage breast cancer were divided into two groups by random number table,and 39 cases in each group.The control group was given modified radical mastectomy,while the observation group was given breast-conserving surgery.The clinical efficacy,surgical conditions and quality of life scores were compared between the two groups.Results The operative time,blood loss,incision length and the average hospital stay of the observation group were (60.23 ± 5.21)min,(44.73 ± 4.22) mL,(4.72 ± 1.02) cm and (9.52 ± 0.86) d,which were significantly lower than those of the control group(t =7.88,8.32,8.89,6.42,all P ＜ 0.05).The postoperative physical factors,mental factors,psychological factors and social factors scores of the observation group were (67.44 ± 3.87),(48.64 ± 3.73),(117.34 ± 8.33)and (61.56 ± 5.32),which were significantly higher than those of the control group (t =5.43,5.87,6.29,7.11,all P ＜ 0.05).The postoperative 3-year local recurrence,distant metastasis and survival rate showed no significant differences between the two groups (x2 =1.22,1.09,1.29,all P ＞ 0.05).Conclusion Breast-conserving surgery in the treatment of early breast cancer has significant effect,and it has less trauma,less bleeding and cosmetic results,and it can be used as the preferred method for the treatment of early breast cancer,which is worth to be further applied in clinical.

OBJECTIVE:To study the active fraction of the seeds of Hovenia Dulcis Thunb.to treat acute alcoholism.METHODES:The seeds of Hovenia Dulcis Thunb.were divided into five parts:superitical fluid extraction(SFE)part,chloroform extraction part,ethyl acetate extraction part,n-butanol extraction part and water extraction part.The effects of the active fraction of the seeds of Hovenia Dulcis Thunb.on ebriety latency,ebriety rate,mortality,and ethanol concentration-time curve of the alcoholism mice were investigated.RESULTS:The ethyl acetate extract could prolong the ebriety latency of the mice,decrease ebriety rate and mortality of the alcoholism mice,and significantly decrease serum ethanol concentrations at 0.5,1,and 1.5h after alcohol administration(P

OBJECTIVE:To determine the content and establish HPCE fingerprints of polysaccharides of the T.jackii seed.METHODS:The content of polysaccharides of the T.jackii seed was determined by phenol-vitriolic colorimetry and the fingerprints of polysaccharides were established by HPCE.RESULTS:The content percentage of polysaccharides in the seed of T.jackii was6.56%,with average recovery rate at98.3%and RSD at2.31(n=5).There were two kinds of polysaccharides in the seed of T.jackii.CONCLUSION:There is no significant difference with regard to the contents and components of polysacc_ harides in the T.jackii seeds from different origins.

Objective: To investigate the viral pathogens in cultivated Crocus sativus. Methods: Viral pathogen identification was carried out by the observation of virus particle morphology and cytopathology as well as the detection of DAS-ELISA, RT-PCR, and sequencing. Results: Linear virus particles of 600-900 nm in length were observed in C. sativus by negative staining under transmission electron microscope (TEM). Bundles of linear virus particles, cylindrical inclusion bodies of subdivision II, and amorphous inclusion bodies were observed in the cells of C. sativus under TEM after ultrathin-section. These observations resembled the cytopathology of infectious Bean yellow mosaic virus (BYMV). Positive result of DAS-ELISA was obtained from the leaves of C. sativus by using monoclonal antiserum against BYMV capsid protein. Positive result of RT-PCR induced by the Potyvirus specific primers (Sprimer and M4) was also obtained. Sequencing after RT-PCR revealed that the viral sequence in this diseased C. sativus had a homology of 99% with the BYMV sequence. Conclusion: The pathogenic virus of this C. sativus disease is identified as BYMV.

Femtosecond laser has been widely used in ophthalmology for its high precision, safety and minimal damage to tissues, and now its application in the corneal refractive surgery, corneal stromal ring implantation and cataract surgery has been more mature.In recent years, it has been actively developed and applied in penetrating keratoplasty, anterior lamellar keratoplasty and corneal endothelial transplantation, and some achievements have been obtained in experimental research and clinical application.While femtosecond laser promotes the development of corneal transplantation surgery, there are still some problems such as limited indications, uncertain efficacy, expensive equipment, lack of big data support and intelligence in personalized design.Continuous optimization and improvement are still needed to ensure postoperative vision, reduce postoperative complications, simplify parameter design and reduce consumption costs.In this paper, the indications, parameter design, clinical effect and limitations of femtosecond laser in keratoplasty were reviewed.

Abstract: An enriched environment refers to conditions that facilitate sensory, cognitive, motor, and social stimulation. Behavioural,cellular and molecular studies have revealed significant effects of enriched environments on rodents and other species. Post-ischemic housing in an enriched environment improves functional outcome, modifies gene activation, induces alterations in the expression of N-methyl-D-aspartic acid (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits, increases dendrite branching and number of dendritic spine in pyramidal neurons in layers II/III in the contra-lateral cortex, induces structural changes in synaptic junctions. Furthermore, it alters lesion-induced progenitor cell differentiation and interacts with necrotic grafting. The idea of environmental enrichment has been applied to clinical stroke rehabilitation and is getting more and more attention. This review will summarize experimental effects of enriched environment on post-ischemic rehabilitation and the clinical application of enriched environment in stroke rehabilitation.

Objective To explore the attentional bias in first-episode depressive patients and the correlations between the attentional bias.Methods In the beginning of the treatment,a total of 34 first-episode depressive patients in treatment-naive and demography matched 40 healthy controls completed the Chinese Emotional Stroop Task that assessed the attentional bias.Results The comparison between depressive patients and healthy controls on the reaction time of negative,neutral and positive-related words were significant ((1185.71±410.60)ms/(754.68±215.36) ms,t=5.775,P＜0.01 ; (1127.92±344.89) ms/(755.37±213.49) ms,t=5.675,P＜0.01 ;(1166.17±395.72) ms/(761.70± 194.66) ms,t=5.708,P＜0.01 respectively).Through analysis of variance,the difference of error numbers among the neutral,negative and positive-related words ((3.41 ± 2.11) times,(2.24±1.65) times,(2.97± 1.17) times) in the depressive patients were significant (F=4.197,P=0.018).The difference of error numbers between negative-related words and neutral-related words were significant through analysis of least significant difference(P＜0.01).Conclusion The results suggest that depressive patients before treatment have attentional bias in negative-related word.Attentional bias in negative-related stimuli may be one of the characteristics of cognitive function in first-episode depressive patients.

MicroRNA (miRNA) controls the proliferation of breast cancer cells in many ways such as cell apotosis,cell cycle and methyltransferase.Also,it controls the metastasis of breast cancer cells in many ways such as microenvironment,epithelial-mesenchymal transition and vessel formation.Large amounts of recent studies indicate that miRNAs are specifically expressed in breast cancer tissue and play important roles in proliferation,metastasis and treatment of breast cancer.

Objective: To observe the effectiveness of neltimicin sulfate in treating the infections of lower respiratory tract.　Methods: 41 cases of lower respiratory tract infections served as observation subjects (treated with netilmicin sulfate 200 mg*d-1, iv drip) while the other 30 cases as control subjects (treated with penicillin 8×109 U*d-1 and ampicillin 8.0 g*d-1, iv drip).　Results: The therapeutic effectiveness was significantly higher in the observation group than that in the control group (P＜0.01). The sensitivity tests of isolates from the sputum showed that 71.1% of the isolates were highly sensitive to netilmicin sulfate, 28.9% moderately sensitive to netilmicin sulfate. The total effectiveness rate was 97.6%.　Conclusion: Netilmicin sulfate is effective in treating the lower respiratory tract infections and causes less side effects.

Objective To summarize the previous work experience and loss of function in the development process of hospital modernization of sterile supply room,and discuss the model of centralized treatment and unified supply.Methods Through investigation in the clinical departments,various roles and regulations of sterile supply room were established,especially the various job responsibilities,operating procedures,disinfection and isolation system,the quality management system,testing system,equipment and apparatus management systems,traceability systems,continuous quality improvement process and goods recall system.The system had performance appraisal,workilow management and other methods to enhance the staff's self-discipline,to strengthen humanities education for sterile supply room staff,strict execution of the standards and norms of Health Ministry,to enhance the legal awareness,establish and improve the sterile supply room management systems and service system.Results Implementation of centralized treatment can effectively prevent nosocomial infection,reduce the spread of pollution to ensure medical safety,reduce staff input that help ensure the professional quality and reduce the economic costs,and promote the professional development of sterile supply.Conclusions Centralized treatment of multiplexing medical apparatus and instruments promotes the all-dimensional development of sterile supply room,which is worthy of wide application.