Browning of white adipose tissue(WAT) is expected to become a possible new therapeutic means for diabetes mellitus and other metabolic diseases in future,because brown adipose tissue (BAT) has attracted scientific interest as an anti-diabetic tissue owning to its ability to dissipate energy as heat.In this article,we reviewed the characteristics of brown fat cells,the major transcription factor and the stimulating factor during browning of WAT,as well as their potential mechanisms and research directions.

Objective To improve the teaching methods for doctor-patient communication skill of medical interns.Methods The present situation and the importance of doctor-patient communication were analyzed.The training model of doctor-patient communication was designed including the aspects of effective training content,training methods and assessment tools,etc.Totally 178 interns in our hospital between June and August 2013 were selected and were divided into two groups.The newly designed training model was applied in students in group A while routine training was applied in students in group B.Doctor-patient communication ability of interns was tested through writing instruments and on-the-spot communicating and investigating satisfaction degree of patients.SPSS 13.0 was applied to do statistical analysis and t test and Wilcoxon were used.Results Score of instrument writing and on-the-spot communication were higher in group A than in group B [Group A:(20.36 ±2.85),(21.18 ± 2.93) ; Group B:(14.79 ± 2.61) and (13.94 ± 3.07)],with statistically significant differences(P=0.000).In addition,according to a survey of 406 patients of group A,367 patients of group B,group A students' satisfaction degree after the training was significantly higher than that group B(59.85％ vs.11.99％)(P=0.000).Conclusion The newly designed doctor-patient communication training model can enhance the doctor-patient communication skills.

The paper first gives an account of the historical backgroumd of the formation of the World Trade Organization and the existing 6 basic principles of it. Then it explains the principles and extent of the opening of the health sector to the outside world and the specific promises made by China in the negotiations involved in its accession to the WTO. Finally the paper speculortes about the effects of Chinas entry into the WTO on domestic hospitals and suggests corresponding strategies.

Objective To investigate whether the combination of glipizide-XL with glucophage was more rational in insulin resistance and insulin secretion in patients with type 2 diabetes mellitus(T2DM).Methods The changes of insulin resistance and insulin secretion were evaluated from variations of IAI(insulin sensitivity index),IR(insulin resistance index.),IS(insulin secretion index)and HBCI(B-ce1l function index)in cases treated with glipigide-XL and glucophage or either for 18 weeks.Results IAI\IR standing for insulin resistance were higher significantly,and IS & HBCI standing for insulin secretion were lower significantly in patients with T2DM than in normal group(P

Objective To explore the relationship between apolipoprotein E ( ApoE ) genotype and diabetic nephropathy (DN) in Chinese Han population of Guangdong province. Methods 63 cases of DN patients were selected as group A; 57 diabetes patients without complications as group B; 55 healthy persons as group C. ApoE genotype of all objects were measured. Results ApoE2 in the group A was the highest, while the differences in other groups were not obvious. Detection of ApoE3 and ApoE4 was not obvious in three groups. The Level of triglyceride (TG) was highest inε2 group, and the level of total cholesterol (TC) and low density lipoprotein LDL-C were highest in ε4 groups. Conclusions In Chinese Han population of Guangdong province , ApoE gene polymorphism and DN are closely interrelated , and the occurrence ofε2 is proportionate to DN.

Objective To investigate the intervention effect of lentivirus expressing human IL-10 (LV-hIL-10) on activated astrocytes.Methods DI TNC1 cell line was treated with different concentrations of lipopolysaccharide (LPS) and time points.The expression of proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukinb-1β (IL-1β) was detected by RT-PCR and ELISA.Moreover,the effect on the expression of proinflammatory cytokines TNF-α and IL-1β was analyzed in DI TNC1 cell lines infected with and without LV-hIL-10.Results The expression of TNF-α and IL-1β was increased in DI TNC1 induced by LPS.The expression of IL-10 was upregulated in DI TNC1 infected with LV-hIL-10.TNF-α and IL-1β were inhibited by IL-10 overexpression in DI TNC1 actived by LPS.Conclusion DI TNC1 is activated by LPS and secretes proinflammatory cytokines TNF-α and IL-1β as immune-like cells,and these activation is inhibited by hIL-10 overexpression.

Objective To analyze the epidemic situation of HPV among gynecology clinical cases and provide scientific support for control and prevention of squamous cell carcinoma .Methods Clinical cases with symptom of increased vaginal secretions and obscure vaginal bleeding and cases diagnosed with cervical squamous cell carcinoma or vaginitis and cervicitis were collected as ob-jectives for HPV testing .Samples were collected by maternity doctors for HPV DNA testing by using the reverse hybrid membrane technology gene chip .All data were analyzed by chi-square test and trend analysis(Z) method .Results 24 .64% of the 4 347 cases were positive for 23 kinds of HPV subtypes .Of the 23 subtypes ,the leading four detection rates were 16th ,43th ,58th and 52th sub-types ,which were 8 .10% (352/4 347) ,3 .93% ,3 .52% and 3 .31% respectively ,and shown significant higher than that of the other 19 subtypes .The detection rates of 6th ,11th ,18th ,33th and 56th subtypes were between 1% to 3% ,whereas the rates of other 14 subtypes were lower than 1% .The detection rates of all the age groups were between 16 .00% ~36 .72% ,and rose with the age in-creasing(Z=3 .32 ,P<0 .01) .The detection rate was the highest in symptoms of increased vaginal secretions and obscure vaginal bleeding ,accounted for 30 .76% (334/1 086) ,secondly in those of increased vaginal secretions ,obscure vaginal bleeding and men-strual were between 20 .68% and 25 .59% .All the 80 cervical squamous cell carcinoma cases were detected HPV ,97 .5% (20/80)of them were high-risk HPV type .The single infection was the most common infection in cervical squamous cell carcinoma cases ,Ac-counted for 75 .5% (60/80)of the total ,and 25% (20/80)of them were multiple infections with 2 to subtypes ,and 70% (56/80) of them were infected with 16th subtypes ,and only2 .5% of them were low-risk type(2/80) .The detection rate of inflammatory disea-ses was 4 .75% (958/3 871) ,and the detection rates were higher in cases of cervicitis and vaginitis and cervicitis complications (be-tween 23 .03% to 28 .38% ) than that of vulvitis (16 .81% )(χ2 >3 .83/6 .63 ,P< 0 .05/0 .01) .Conclusion The detection rate of 16th subtypes HPV was the highest for gynecology clinical cases ,secondly were the 43th ,58th and 52th subtypes .The detection rate of HPV was the highest in symptoms of increased vaginal secretions ,obscure vaginal bleeding and vaginitis .The detection rate rose with the age increasing .It was concluded that elderly cases with increased vaginal secretions ,obscure vaginal bleeding and vagi-nitis were the biggest targets for control and prevention of HPV and cervical squamous cell carcinoma .

As the curriculum was unreasonable and teaching approac h was simple in the course of pharmaceutical analysis,the course curriculum was reconstructed and optimized to be more advanced and more scientific.Reasonable teaching approaches will be adopted according to different teaching contents and diverse teaching model which is benefit to the cultivation of students' innovative capability will be constructed.

Objective To explore the clinical efficacy and side effects of paclitaxel combined with cisplatin chemotherapy for patients with locally advanced cervical cancer. Methods From May 2008 to May 2009,78 patients with locally advanced cervical cancer in our hos-pital were randomly divided into PF group (39 cases) and Taxol group (39 cases). Patients from PF group were treated with cisplatin com-bined 5-Fu while patients in Taxol group were cured with paclitaxel combined with cisplatin. After chemotherapy,all patients were given anes-thesia extensive hysterectomy and pelvic lymph node dissection. The clinical efficacy,side effects and three-year survival of 2 groups were as-sessed and compared. Results The main adverse reactions of PF group were gastrointestinal reactions,oral mucositis and bone marrow sup-pression;the major side effects of Taxol group were bone marrow suppression,oral mucositis and renal toxicity. After chemotherapy,the com-plete remission rate of Taxol group reached 30. 77%,which was higher than that of PF group 10. 26% (χ2 =5. 032,P=0. 025). The average survival time of PF group and Taxol group were 33. 5 months and 34. 7 months,the three-year survival were 59. 6% and 70. 2%,respectively (χ2 =1. 859,P=0. 173),and the difference was not statistically significant. Conclusion The adverse effects of different chemotherapy were observed in 2 groups. The clinical efficacy of Taxol group were better than that of PF group.

The resting cells of Citrobacter freundii 48003 3 expressing high tyrosine phenol lyase activity under the inducing of L tyrosine were used for L DOPA synthesis from catechol, pyruvate and ammonia In this paper, the effects of temperature, pH and substrate concentrations on the synthesis of L DOPA were studied At the optimal conditions of reaction, 9 5g/L of L DOPA was obtained in 12h