Objective :To study the effects of ascorbic acid(vitamin C) on PKC -?1 activity in kidneys of diabetic rats. Methods :30 Wistar rats were randomly divided into three groups: Control group (group C), streptozotocin - induced diabetic rats group(group 13) and diabetic rats treated with ascorbic acid 90mg/(kg?d)(group DT). The concentration of ascorbic acid in plasma and kidney were measured at 9 week. The activity of PKC - ?1 was observed by immunofluorescence technique and laser confocal microscope. Results : Compared with group C,the level of ascorbic acid in the plasma and kidney of group D decreased significantly (P

Objective To detect HBV outer membrane large protein(HBV-LP) and HBV-DNA, and to explore their correlation in judging the pathogenetic condition and HBV replication, as well as its clinical significance. Methods Serum level of HBV-DNA was quantitatively detected by u-sing real-time polymerase chain reaction, and while HBV-LP and HBV markers were measured with enzyme-linked immunosorbent assay(ELISA) in infected sera from 238 patients with hepatitis B. Re-suits There was significant difference of detection rate between HBV-DNA and HBV-LP(P<0. 05).The expression of HBV-DNA and HBV-LP had significantly difference among various patterns of HBV serological markers(P<0.05). HBV-LP expression was markedly correlated with the logarithm of HBV-DNA level(r=0. 677,P<0.01). Conclusion There is a prefect correlation between the copies of HBV-DNA and the level of HBV-LP, but HBV-LP expression can not still reflect the status ofHBV replication.

Objective:To probe the relationship between hepatitis B virus(HBV) variation and TCM syndrome types in HBeAg-negative patient of chronic hepatitis B.Methods:Eighty-four cases of HBeAg-negative patient of chronic hepatitis B were divided into 5 syndrome types according to TCM differentiation.C gene promoter (BCP area) and pre-C gene termination variation in HBV were detected with gene chip for HBV mutation detection.Results:There was no significant correlativity between generation of HBV variation and TCM syndrome types in HBeAg-negative patient of hepatitis B as compared with HBeAg-positive patient;The variation rate of the BCP area and/or the pre-C area was 34.5% in prevailing excess syndrome group and 65.5% in the prevailing deficiency group,the former being higher than the latter.Conclusion:There is correlativity between variation of BCP area and/or pre-C area and TCM syndrome types,with the variation rate of the excess syndrome being lower than that of the deficiency syndrome.

The morbidity of hyperuricemia(HUA) has showed an increasing tendency in recent years,along with the improved social living conditions.HUA has been proved to be closely related to kidney diseases,metabolic diseases and cardiovascular diseases.However,people pay little attention to the relationship between uric acid and renal diseases in pediatrics,and the relevant studies and progress are lacking.Now a review is made about the metabolism of uric acid,the etiology and pathogenesis of HUA,and the relationship between HUA and kidney diseases or drugs,in order to pay attention to the relationship between HUA and kidney diseases.

Objective: To investigate the rate of eating disorder in B ei jing girls Method: 38 girls were screened out from 261 female adolescents of two schools in Beijing by self-made questionnaire They received structured interview Results: Three of the 38 were diagnosed as eating disorder NOS (with bulimia) according to DSM-IV Conclusion: The detected rate of eating disorder among Beijing female adolescent is 11%

Objective:To reduce the incidence of nosocomial infection via application of Quality Control Circle ( QCC) .Methods:After organizing QCC group , the factors causing nosocomial infection in our hospital were inves-tigated, then the corrective measures were also discussed .Results:The incidence of nosocomial infection was re-duced from 18.3 cases per month to 5.7 cases per month after the implementation of QCC .Conclusion:QCC can not only reduce the incidence of nosocomial infection , but also improve the responsibility and enthusiasm of the staff.It makes the management of nosocomial infection more standard .QCC can also increase the cohesion and the ethical concepts of nurses .It's worth of promoting in routine nursing .

BACKGROUND:Scaffold materials are the important part of tissue engineering bone, and the ideal biodegradation speed of the scaffold materials can be in accordance with the regeneration speed of bone formation in order to achieve a long-term stability. OBJECTIVE:To summarize the biodegradability of different types of currently available scaffold materials and its relationship with bone formation. METHODS:The PubMed and CNKI database were searched by the first author using the key words of “tissue engineering bone, composite scaffold, scaffold degradation” in English and Chinese for articles addressing biodegradability of scaffold materials and its relationship with bone formation published during January 1996 and December 2013. RESULTS AND CONCLUSION:Many scaffold materials under organic, inorganic and organic-inorganic categories have been available and the current studies focus on inorganic materials and organic-inorganic composite materials.In vivo andin vitro studies indicate the different characteristics and relative merits of these scaffold materials. Organic materials have a relatively slow degradation rate while inorganic materials can completely degrade but have an insufficient mechanical property. For organic-inorganic composite materials, further studies are needed.

Objective To explore the effects of enalapril on spatial learning and memory,and to identify synaptophysin expression in hippocampus of streptozotocin-diabetic rats.Methods Forty rats were randomly divided into control group,diabetic model group and the enalapril treatment group.Streptozotocin-diabetic rat model was developed.The diabetic rats were treated with enalapril for 12 weeks.Then,the learning and memory abilities of rats were tested with Morris water maze;mRNA and protein expression of synaptophysin in hippocampus was detected by RT-PCR and immunostaining.Results Compared with diabetic model group,the enalapril treatment group showed a significant decrease in the mean time of escape latencies(P

Through the questionnaire survey of community residents,the paper studies the factors influencing users' acquisition of health information from libraries in medical universities and colleges and provides reference and suggestions for socialized services of these libraries.As indicated by the results,the influencing factors include the distance,motivation,environment,limitation and time.On this basis,it proposes solutions,including the improvement of the service mechanism,the utilization of advantages of medical library resources and the establishment of the service system for community residents centering on medical libraries.

OBJECTIVE:To explore the effects of vitamin C in different dosages on the activity of aldose reductase(AR) in renal cortex in rats with streptozotocin-induced diabetes METHODS:50 male Wistar rats(195?20)g were randomly divided into following groups:group C(control rats);group D(diabetic rats);group DT1(diabetic rats treated with vitamin C 30mg/(kg?d);group DT2(diabetic rats treated with vitamin C 90mg/(kg?d);group DT3(diabetic rats treated with vitamin C 270mg/(kg?d);The activities of AR in renal cortex and the concentrations of vitamin C in plasma and renal cortex were assayed 9 weeks after treatment RESULTS:Compared with group C,the levels of vitamin C in plasma and renal cortex decreased significantly(P