Objective To study the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) detection in early skin tissue fluid of syphilis.Methods A total of 40 patients of suspected syphilis who received therapy from September 2014 to September 2016 in our hospital were selected in this study.Five mL venous blood samples were collected in all the patients,and detected by toluidine red unheated serum test (TRUST) and treponema pallidum antibody enzyme-linked immunosorbent assay (ELISA),and skin tissue fluid were collected and performed FQ-PCR detection,all patients were treated with benzylpenicillin for 3 and 6 months,then detected again,the conversion rates were record.Results There was no significant difference in the detection rate of ELISA and FQ-PCR[97.50%(39/40) vs.95.00%(38/40),P>0.05].The total detection rate of ELISA and FQ-PCR were significantly higher than that of TRUST[97.50%(39/40) vs.67.50%(27/40),95.00%(38/40) vs.67.50%(27/40),P<0.05].After 3 and 6 months of treatment,negative rates of ELISA and FQ-PCR were significantly higher than that of TRUST(P<0.05),there was no significant difference on negative rate between FQ-PCR and ELISA (P>0.05).In the FQ-PCR detection results,the average value of TP-DNA was significantly decreased after treatment,there were significant differences in the phase Ⅰ,phase Ⅱ compared with before treatment(P<0.05).Conclusion FQ-PCR could be used to measure treponema pallidum (TP-DNA) effectively in early stage,it′s conducive to the diagnosis of syphilis,the clinical application value is high.

Objective To explore the clinical significance of combined detection of carbohydrate antigen(CA)125,CA153,carcinoembryonic antigen(CEA) and tumor specific growth factor(TSGF) in diagnosis for breast cancer.Methods A total of 125 patients with breast cancer were recruited as objects in this study from march 2015 to march 2016,65 patients in breast cancer group,60 patients in benign breast disease group,meanwhile 55 healthy person were enrolled in the control group.Serum tumor markers such as CA125,CA153,CEA and TSGF were detected and compared in the three groups.Results The serum CA125,CA153,CEA and TSGF levels in the breast cancer group were significant higher than those of benign breast disease group and healthy group,the differences were statistical significant(P<0.05).At the same time,the sensitivity and specificity of joint detection of four kinds of serum tumor marker were 90.2% and 88.9%,which were higher than those of single serum tumor marker detection(χ2=26.12,P<0.05).Conclusion The four kinds of serum tumor markers combined testing not only increases the sensitivity of breast cancer diagnosis,but also improved the specificity of diagnosis of breast cancer.

In this research, Casuarina eguisetifolia Linn was used to verify the broadly suitability of DNA bar-codes in identification of Li-medicine plants and systematic development of species. The genomic DNA of 22 samples collected C. eguisetifolia and its adulterants were amplified by 4 pairs of primers respectively (ITS (inter-nal transcribed spacer), ITS2 (internal transcribed spacer 2), trnH-psbA , rbcL) and sequenced bi-directionally. Obtained sequences were assembled using CodonCode Aligner. The dates were analysised using MEGA5.1 in ac-cordance with the kimura 2-parameter (K2P) model. The neighbor-joining (NJ) phylogenetic trees were construct-ed. Our study demonstrated the efficacy of ITS/ITS2 to distinguish between C. eguisetifolia and other adulterants species at the molecular level. Comparative to the primer of trnH-psbA and rbcL, there was a obviously DNA gap. The NJ trees showed that the several species of Casuarina can be classified to same types to show a obvi-ously monophyly, which the nearest family was Guttiferae. Therefore, ITS/ITS2 regions can accurately distinguish the original plant of Li-medicine. The systematic evolution of Casuarina can be verified in the molecular level.

Objective To observe and analyze the occurrence and related factors of pulmonary fibrosis caused by dust exposure in Shenzhen city in 2014 ,and to explore the preventive measures .Methods 64 cases of patients with pneumoconiosis from January 1st ,2014 to December 31st were selected as the study objects ,the factors affecting the development of pneumoconiosis and the re‐lated factors of complications were compared and analyzed ,the preventing measures were discussed .Results the proportion of pneumoconiosis stage Ⅱ and stage Ⅲ and the incidence of complications were increased with the working age of dust‐exposed work ,the differences were statistically significant(P<0 .05) .Compared with patients who used anti‐dust respirator and were trea‐ted in time ,the patients used common respirator and not treated in time had higher proportion of pneumoconiosis stage Ⅱ and stageⅢ and the incidence of complications ,the differences were statistically significant(P<0 .05) .The incidence of complications associ‐ated with pneumoconiosis such as tuberculosis ,pulmonary emphysema ,pulmonary heart disease were higher in patients with more than 20 working years than those with 10- <16 and 16-20 years ,the differences were statistically significant(P<0 .05) .Conclu‐sion Working years of dust‐exposed work ,personal protective measures ,timely treatment are major influence factors for the occur‐rence and development of pneumoconiosis and related complications .Strengthening the engineering and technical measures for re‐ducing dust in dust‐exposed work ,personal protection and health education ,regular physical examination are important means to prevent the occurrence and development of pulmonary fibrosis caused by dust‐exposed work .