Objective: To establish the quality standard for Heluoshugan Capsules(Rhizoma Atractylodis macrocephalae, Radix Paeoniae alba, Rhizoma cyperi, Rhizoma sparganii, Radix et Rhizoma rhei, Radix Angelicae Sinensis, etc.). Methods: Paeoniflorin of Radix Paeoniae Alba was determined by HPLC. Rhizoma cyperi, Radix Paeoniae alba, Radix aucklandiae and Rhizoma sparganii were identified by microscopic identification, and Radix et Rhizoma Rehei was identified by TLC. Results: The average recovery rate of paeoniflorin was 99.18%, and RSD was 2.3% . The microscopic characteristics were obvious. The TLC sports developed were fairly clear, and the blank test showed no interference. Conclusion: The method developed is accurate and the reproducibility is good. The method can be used for quality control of the Heluoshugan C apsules.