Objective To evaluate the treatment effect of negative pressure wound therapy ( NPWT) followed by ultrasonic debridement surgical system ( UDSS) in patients with diabetic foot. Methods Forty-seven diabetic foot ulcer patients( WagnerⅡ~Ⅲ) were divided into two groups,of which 26 patients treated with UDSS combined NPWT,and 21 patients treated with traditional surgical debridement combined NPWT were control group. Cure rate, markedly effective rate and adverse reaction and complication were compared between two groups. Results Compared with control group,the experiment group had higher markedly effective rate in the first week(P<0. 05),and healing rates were higher in the 2th,4th,6th,12th weeks (P<0. 05). There were no differences in the adverse reactions and complications between the two groups. Conclusion UDSS combined with NPWT can accelerate wound cure,may play an important role for the treatment of diabetic foot ulcer.

Objective To investigate the therapeutic effect of different methods of dressing on the wound after local debridement of dia-betic foot. Methods A total of 53 patients which underwent local debridement of diabetic foot were divided into control group and treatment group. Patients in control group were dressed on traditional measurement,while patients in the treatment group were dressed on the external medicinal wine for the diabetic foot basic on the traditional treatment. The local transcutaneous oxygen partial pressure,ulcer healing rate,av-erage healing time and the amputation rate were observed. Results The healing rate and percutaneous oxygen partial pressure of the treat-ment group were significantly increased than those in the control group (P<0. 05). The average healing time and amputation rate of the treat-ment group were significantly less than those in the control group (P<0. 05). Conclusion Dressing on external medicinal wine after local debridement of diabetic foot can improve the wound healing in the diabetic foot.

OBJECTIVE: To explore the effect of high glucose and lipid intervention on islet cell apoptosis through the inhibition of prostate apoptosis response factor-4 (Par-4) expression and the underlying mechanisms. METHODS: The mice islet β cells (NIT-1 cells) were randomly divided into a control group, a Par-4 inhibited group, a glucose and fatty acid intervented group and a glucose and fatty acid intervented+Par-4 inhibited group. Cell apoptosis was detected by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL), and the protein expression levels of Par-4 and glucose regulated protein 78 (GRP78) were detected by Western blot. RESULTS: Compared with the control group [(3.14 ± 1.08)%], the apoptosis rate of islet beta cell [(33.82 ± 3.15)%] in the glucose and fatty acid intervented group was significantly increased accompanied by the dramatically elevated Par-4 and GRP78 expression (both P<0.05). Compared with the glucose and fatty acid intervented group, the apoptosis rate in glucose and fatty acid intervented+Par-4 inhibit group [(18.3 4 ± 2.11)%] was significantly decreased concomitant with the significantly decreased Par-4 and GRP78 expression (both P<0.05). CONCLUSION The glucose and fatty acid-induced apoptosis of mice islet β cells could be improved through the inhibition of Par-4 expression, which might be related to reduction of endoplasmic reticulum stress.
Animals ; Apoptosis ; Apoptosis Regulatory Proteins ; metabolism ; Cell Line ; Fatty Acids ; Gene Expression Regulation ; Glucose ; Heat-Shock Proteins ; metabolism ; In Situ Nick-End Labeling ; Islets of Langerhans ; cytology ; Mice