Objective To study the relative specificity of Yanglingquan on relieving spastic gastrocnemius. Methods According to evidence-based medicine/DME principle, the effect of Yanglingquan in relieving gastrocnemius spasm of old women was studied and evaluated by standard diagnostic and efficacy criteria in random blind multi-center clinical test, with Yanglingquan as the observation group and non-related acupoint Quchi as control group. The effect was investigated by muscle spasm rehabilitation assessment methods. Results There were significant differences in the grade integral of symptoms of both groups between before and after treatment (P 0.05). Conclusion The effect of Yanglingquan in relieving gastrocnemius spasm was better than Quchi, and gastrocnemius spasm isn’t relevant to the content of K+, Na+, Cl-, Ca2+ in blood.

Pancreatic adenocarcinoma (PAAD) is one of the most lethal malignancies. Although gemcitabine (GEM) is a standard treatment for PAAD, resistance limits its application and therapy. Secoemestrin C (Sec C) is a natural compound from the endophytic fungus Emericella, and its anticancer activity has not been investigated since it was isolated. Our research is the first to indicate that Sec C is a broad-spectrum anticancer agent and could exhibit potently similar anticancer activity both in GEM-resistant and GEM-sensitive PAAD cells. Interestingly, Sec C exerted a rapid growth-inhibiting effect (80% death at 6 h), which might be beneficial for patients who need rapid tumor shrinkage before surgery. Liquid chromatography/mass spectrometry and N-acetyl-l-cysteine (NAC) reverse assays show that Sec C sulfates cysteines to disrupt disulfide-bonds formation in endoplasmic reticulum (ER) proteins to cause protein misfolding, leading to ER stress and disorder of lipid biosynthesis. Microarray data and subsequent assays show that ER stress-mediated ER-associated degradation (ERAD) ubiquitinates and downregulates YAP to enhance ER stress via destruction complex (YAP-Axin-GSK-βTrCP), which also elucidates a unique degrading style for YAP. Potent anticancer activity in GEM-resistant cells and low toxicity make Sec C a promising anti-PAAD candidate.