OBJECTIVE:To establish an HPLC method for the determination of triterpenoid in the roots of Actinidia deliciosa from Guangxi. METHODS: With the content of 2?,3?,24-trihydroxyursa-12-en-28-oic acid used as index. The separation was performed on Thermo Hypersil BDS C18 column (250 mm?4.6 mm,5 ?m) with 2?,3?,24-trihydroxyursa-12-en-28-oic acid as standard substance. The mobile phase consisted of methanol-0.2% phosphoric acid (73 ∶ 27) with column temperature set at 20 ℃. The flow rate was set at 1.0 mL?min-1 and detection wavelength was 210 nm. RESULTS: The linear range of 2?,3?,24-trihydroxyursa-12-en-28-oic acid were 0.025～0.200 mg?mL-1(r=0.999 8). The average recovery was 99.79%(RSD=1.54%,n=6). CONCLUSION: The method is accurate, reliable and reproducible for the quality control of the roots of A. deliciosa from Guangxi.