Objective: To analyze the influencing factors of death in patients with coronavirus disease 2019 (COVID-19) and provide a reference for clinically reducing the mortality of patients with COVID-19. Methods: Based on the retrospective analysis of the case data of all patients with COVID-19 admitted in our hospital from January 17th to February 25th, 2020, and the sex, age, whether or not to take Chinese medicine preparations during hospitalization, clinical classification, comorbidities, nucleic acid test results, admission time, discharge and other relevant indicators were collected for statistical analysis. Results: During the period of COVID-19, the number of patients of COVID-19 in our hospital gradually increased. At the epidemic peak period, 904 patients with COVID-19 were treated simultaneously. From January 17th to February 25th, 2020, a total of 1 305 patients with COVID-19 were treated in our hospital, including 632 males and 673 females, and the male to female ratio was 1:1.06. The age distribution ranged from 7 to 111 years, with a median of 63 (51, 70) years old. There was no significant difference in the age distribution of patients between different genders (Z = 1.217, P = 0.224). The results of univariate analysis showed that the patient's gender, age, whether to take Chinese medicine preparations during treatment, clinical classification, whether combined with underlying diseases (hypertension, coronary heart disease, diabetes, tumors and uremia) and nucleic acid test results all could affect patients fatality rate. Logistics multivariate regression analysis found that taking traditional Chinese medicine preparations, clinical classification, and whether combined with underlying diseases were independent risk factors for death in patients with COVID-19. Conclusion: The death of patients with COVID-19 is related to various factors. Reducing the occurrence of critical illness, controlling underlying diseases, stabilizing blood pressure and blood sugar, actively improving cardio-cerebral vascular conditions, and stabilizing renal function are main measures to improve the therapeutic effect, and early application of traditional Chinese medicine treatment also plays a very important role in reducing the mortality of patients with COVID-19.

Child ; Child, Preschool ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Interleukin-6 ; urine ; Male ; Nephritis ; drug therapy ; urine ; Purpura, Schoenlein-Henoch ; drug therapy ; urine ; Tablets

【Objective】 To investigate the situation of hepatitis E virus(HEV) infection among voluntary blood donors in Wuhan area and provide evidences for enhancing blood screening strategies. 【Methods】 HEV nucleic acid detection(NAT) was performed on blood samples from eligible blood donors in Wuhan from November to December 2020. The testing results were analyzed, and the blood donors with repeated reactive results were followed up to clarify the status of infection. 【Results】 Routine screening was performed on 17 409 blood samples from November to December 2020. A total of 17 322 blood samples of eligible blood donors were tested for HEV NAT, and one case of HEV RNA reactivity was detected. The results from the follow-ups showed that the blood donor should be in the window period of HEV seroconversion. The current HEV infection rate of voluntary blood donors in Wuhan arewas 0.058‰(1/17 322), which was lower than other domestic areas. 【Conclusion】 The current HEV infection rate of voluntary blood donors was at a relatively low prevalence level in Wuhan area. Selective blood screening strategies can be taken to further reduce potential risk of blood transfusion infection with hepatitis E virus.

【Objective】 To explore biosafety countermeasures and optimize the sample detection procedures for the COVID-19 in tertiary hospitals in Wuhan, so as to provide effective protection for blood transfusion laboratory. 【Methods】 Referring to General Requirements for Laboratory Biosafety, Novel Coronavirus Pneumonia Prevention and Control Program (Fourth Edition), Technical Guide for Laboratory Testing of the Novel Coronavirus Pneumonia (Fourth Edition), the Novel Coronavirus Laboratory Biology Safety Guidelines (Second Edition) and other technical guidelines and expert consensus, relevant biosafety protection measures and testing procedures were formulated in combination of the layout and conditions of our laboratory. 【Results】 The biosafety zoning of blood transfusion laboratory, procedures for sample receiving, testing, and sterilizing, as well as the protection procedures for workers entering and exiting the laboratory were formulated, with good adherence with the relevant laws, regulations and technical specifications concerning COVID-19. During the prevention and treatment of the COVID-19, the work of blood transfusion department was carried out orderly, without any medical staff infection. 【Conclusion】 The biosafety protection measures and sample detection procedures in compliance with relevant laws, regulations and technical specifications, taking the actual condition of our laboratory into consideration, can effectively prevent and control the infection of COVID-19 and protect the health of medical staff.

CALR mutations are discovered in classic myeloproliferative neoplasms (MPN) as a new biomarker very recently.CALR mutations occur in about 20 ％-35 ％ essential thrombocythemia and primary myelofibrosis and are associated with some clinical features and favorable prognostic impact.This article reviews biological characteristics and clinical implications of CALR mutations in classic MPN.

【Objective】 To analyze the relevant studies on blood donor cohort and explore the current research status, research hotspots and development trends of blood donor cohort studies abroad. 【Methods】 Based on the Web of Science Core Collection, the literature related to blood donor cohort was searched. After screening and data processing, bibliometric analysis was carried out from the volume of literature trends, disciplines, authors, institutional distribution and other aspects of the included literature. At the same time, CiteSpace 5.6R5 software was used to carry out visual analysis of countries/regions, literature co-citation, keyword co-occurrence, keyword clustering and keyword emergence. Results A total of 672 papers (654 research papers, 18 review papers) were included. The total number of papers published in the field of blood donor cohort study showed a steady upward trend since 1991, the fastest growth was between 2019 and 2020. The top three cited authors were Kaaks R (1 301 citations), Rinaldi S (1 186 citations) and Riboli E (1 130 citations); the top three institutions were RLUK library in the UK, the University of California and the University of Copenhagen; the top three countries/regions in the volume of literature were the United States (176 papers), Germany (64 papers) and France (54 papers), and they all co-operated closely with other countries. The top five keywords were "blood donor" , "prevalence" , "infection" , "risk" and "antibody" . A total of 19 clusters were obtained by keyword cluster analysis, it was reflected in the immune mechanism and prevalence of blood-borne diseases, the relationship between blood donation behavior and non-communicable disease and its influencing factors, and the classification of the characteristics of blood donors. "Non-hepatitis B" was the keyword with the greatest burst intensity. The keywords that have burst out in recent years and continue to this day include "donation" , "seroprev-alence" , "donor" and "management" . Conclusion At present, the cohort study of blood donors has been paid more and more attention in the world, and its research trends mainly focus on the study of transfusion-transmitted disease risk. However, more researches begin to pay attention to the health problems and influencing factors of blood donors or recipients, which provides the research ideas and directions for establishing cohort study among blood donors in China.

Objective To investigate the anti-hyperuricemia effects of Bixie deacidification fang on hyperuricemia mice and its mechanism of renal protein transport. Methods The effects of Bixie deacidification fang were investigated on hyperuricemia mice induced by potassium oxonate. Bixie deacidification fang was administered to hyperuricemia mice daily at doses of 220, 440 and 880 mg/kg for 10 days, and allopurinol (5mg/kg) was given as positive control. Serum and urine levels of uric acid and creatinine were determined by colorimetric method. Simultaneously, protein levels of urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1) in the kidney were analyzed by Western blot. Results Compared with the model group, high-dose of Bixie deacidification fang inhibited xanthine oxidase (XOD) activities in serum (18.12±1.33 u/L) and that in liver (70.15±5.20 u/g protein) (P<0.05), decrease levels of serum uric acid (2.04 ± 0.64mg/L) (P<0.05) and serum creatinine (0.35±0.18µmol/L) and blood urea nitrogen (BUN)(8.83±0.71mmol/L) (P<0.05), ncreased levels of urine uric acid (38.34±8.23mg/L), urine creatinine (34.38±1.98mmol/L), down-regulated of URAT1 and up-regulated of OAT1 protein expressions (P<0.05) in the renal tissue of hyperuricemia mice. Conclusion Bixie deacidification fang recipe may promote the excretion of uric acid in the kidney by up-regulating the expression of OAT1 protein to promote the excretion of uric acid, and down-regulating the expression of URAT1 protein to inhibit the reabsorption of uric acid.

Objective: To explore the rats bone biomechanics improvement action after exercise combined with traditional Chinese medicine compound of castration. Method: A total of 100 SPF SD female rats, aged 180 days, were randomly divided into blank group，model group，exercise，traditional Chinese medicine compound，sports combined with traditional Chinese medicine compound group, 20 in each group. Followed conventional breeding 14 days after surgery. 2ml 0.9% sodium chloride was filled to stomach daily for model group and exercise group. 2ml traditional Chinese medicine compound solution was filled to the stomach daily for the traditional Chinese medicine compound and sports combined with traditional Chinese medicine compound group. Besides, the rats were forced to running on flat treadmill 20 minutes daily at speed of 5m/min for exercise group and sports combined with traditional Chinese medicine compound group. After 12 weeks treatment, bilateral femur were collected, attached muscle and connective tissue were took out. Then three-point bending test, microstructure observation, and static parameters of bone trabecula test were completed. Result: BMD, maximal load of femur, and static parameters of bone trabecula test for model group, blank group, exercise group, Chinese medicine compound group, exercise combined with traditional Chinese medicine compound group all have obvious difference from the model group (P<0.01 or P<0.05). Conclusion: Exercise combined with traditional Chinese medicine compound is beneficial for treatment of osteoporosis in castrated rats.

Now China vigorously promoted the reform of the disease prevention and control system and the construction of the public health system. In the context of the reform of the disease prevention and control system, grassroots disease prevention and control institutions have been greatly improved in terms of the number and structure of personnel, the configuration of facilities and equipment, the construction of information technology, and the ability of laboratory testing. However, it is still a challenge for grassroots disease prevention and control institutions to achieve long-term development under the supportive development policy of ^“one type of protection and two types of management^”. Paper in Wuhan district of institution of disease prevention and control, for example, by analyzing its current working train of thought, puts forward the basic institution of disease prevention and control should be in the internal management level, social level and system level under the three-dimensional development ideas, fully show the technical characteristics, improve the ability of the business, to provide more diversified public health services to the public, may be able to get long-term, stable and continued development.

【Objective】 To study the serological and molecular mechanism of a case of para-Bombay blood group caused by 236delG mutation of FUT1 gene and investigate the pedigree. 【Methods】 The ABO, H and Lewis antigens of the proband and her family members were detected serologically, and the ABO blood group was confirmed by gene testing. The FUT1 gene was amplified by PCR and then sequenced. The structure of FUT1 236delG enzyme of the proband was simulated in 3D by SwissModel online server. 【Results】 Serological results showed that the proband was rare para-Bombay ABhm, Le(a-b-). Her father and mother was type A and type B, respectively. The gene results showed that the proband was type AB, while her father and mother was type A and type B, respectively. The sequencing results showed that the proband had 236delG/551_552delAG gene mutation, while her mother had 236delG FUT1 gene mutation, and her father had 551_552delAG FUT1 gene mutation. The 3D simulation of the enzyme structure of the proband FUT1 236delG showed that the translated product was an alpha helix structure with no actual function. 【Conclusion】 The 236delG mutation is a new discovered mutation in FUT1 genotype, with 551_ 552delAG mutation(FUT1^* 01N.06 genotype), which can result in the generation of para-Bombay blood group.