Objective To develop a new method of preparing anti-Rh(D) reagent serum in order to help overcome the shortage of Rh(D) negative red blood cells(RBCs). Methods Anti-Rh(D) containing type A and B sera were mixed. Through dilution and neutralization, the titer of complete antibodies decreased to 2. Anti-Rh(D) saturated Rh(D)positive RBCs were prepared by adding anti-Rh(D) containing serum to Rh(D) positive RBCs so that all the Rh factors on the cells were saturated. The anti-Rh(D) saturated type A and type B RBCs were added to the mixed plasma to absorb the remaining anti-A and anti-B. Results The acquired regent sera had a high anti-Rh(D) titer with a high specificity. Conclusion Anti-Rh(D) saturated Rh(D) positive RBCs absorption after neutralization can help with the production of human origin anti-Rh(D) reagent serum and save a large amount of Rh(D) negative evythvocytes.

OBJECTIVE To study the sterilization by gamma radiation on the cytokine levels in manually manipulated platelet concentrates, and its practicability. METHODS Manually manipulated platelet concentrates were irradiated by gamma ray at 25 Gy. Detected the quantity of cytokine levels after irradiation on dd 0, 1, 3, and 5. Platelet counts and pH value were detected on the d0 and d5. RESULTS The contents of cytokines increased with storage time both in irradiation and control groups. But the contents of cytokines in the control group increased more significantly than in the irradiation one (P