OBJECTIVE:To study the liver damage mechanism in mice caused by the incompatibility of Daphne genkwa com-bined with Glycyrrhiza uralensis in aspect of liver transporter. METHODS:40 mice were equally randomized into a normal control (isometric normal saline)group,a group of G. uralensis [15 g(medicinal matierias)/kg],a group of D. genkwa [15 g(medicinal tatierias)/kg],a group of D. genkwa with G. uralensis in the ratio of 1∶1 [15 g(crude drug)/kg],a group of D. genkwa with G. uralensis in the ratio of 1∶3 [15 g(medicinal matierias)/kg](n=8). The mice were given the corresponding drug,ig,once a day for 7 consecutive days. HE staining was performed and then the pathomorphology of liver tissues were observed under the light mi-croscope,and calculation was made for pathological grading. Western blot method was employed to determine the protein expre-ssion of the transporter Ntcp protein in the livers of mice. The contents of total bile acids(TBA)in livers of mice were determined. RESULTS:Compared to the mice in the normal control group,those in the group of 1∶1 and 1∶3 demonstrated higher protein ex-pression of Ntcp. In the group of 1∶1,the mice with grade“+++”hepatocyte degeneration were more (8). The mice with grade“+++”and“++”hepatocyte degeneration in the groups of 1∶3 were more (2 and 8 respectively). CONCLUSIONS:D. genkwa combined with G. uralensis can induce liver damage in mice by a mechanism which may be related to the accumulation of a large amount of TBA in the liver as a result of the increase in the expression of Ntcp in mice.

Purpose: To report 2 cases of papillary thyroid carcinoma occurring in a thyroglossal duct remnant, and to discuss the diagnostic and therapeutic methods by reviewing the literature. Methods: An asymptomatic midline mass occurring in the upper neck was the sole presenting complaint in 2 cases. The preoperative evaluation included a complete head and neck examination, B-ultra sound examination and FNAB. The Sistrunk procedure was done. A lobectomy( case 1) and a lumpectomy ( case 2) were performed respectively, because of the abnormality found in their thyroid gland. A modified neck dissection was performed in case 2 because of regional lymphadenopathy. The following histologic studies were carried out on tissues with HE stain. Results: Diagnoses of thyroglossal duct carcinoma were made by several pathologists. Tissues from thyroid were first diagnosed as nodular goiter in case 1 and thyroid adenoma in case 2. In case 2, one positive lymph node and invasion to the hyoid bone was found. Conclusions: Malignant lesions are rare in the thyroglossal duct remnant. The diagnostic criteria is acknowledged. Resection of the thyroglossal duct carcinoma by the Sistrunk procedure is an adequate surgical approach. But the controversy about further treatment will continue because of the lack of large series of patients and the 10 to 20 year follow-up.

The Medline and CNKI databases were searched with the key words of radiation-induced myocardial damage,damage pathway,pathogenesis,and intervention,and 37 articles were obtained.The pathogenesis of radiation-induced myocardial damage may be related to various mechanisms such as oxidative stress,transforming growth factor-β,renin-angiotensin system,mast cells,and endothelial dysfunction.The pathogenesis of radiation-induced myocardial damage is a complex process involving various mechanisms,and currently,there are ongoing studies on the cellular and molecular mechanisms involved in its pathogenesis and interventions.

[Objective] In order to optimize the best condition for the water extraction process of Polysaccharides from Pleurotus ostreatus.[Methods]The single factor experiments were conducted,in which,the effects of radio of liquor to solid,irradiation time,temperature were studied separately.The orthogonal experiments were conducted to find out the appropriate operating conditions.[Results] The appropriate operating conditions are as follows:the radio of liquor to solid is 1:35.The irradiation time is 3h.and the temperature is 90oC.Under these conditions the average yield of Polysaccharides is 2.84%.[Conclusion]It is an ideal means for extracting Polysaccharides from Pleurotus ostreatus,with short time,less cost and sensitivity.

Objective To provide technical support and scientific basis for establishing the evaluation index system for rural area in Chongqing city .Methods We formulated first draft of evaluation index system and then used the Delphi method to analyse this draft based on expert evaluation .Evaluation index system were established after second operation .Results “Familiarity ,security , effectiveness ,economy ,simplification ,the number of cases ,equipment and suitability for popularization and application”were finally taken as indexes of evaluation index system .Conclusion Useing Delphi expert consultation method ,we could establish a reasonable evaluation index system of Chongqing city .

This paper reports the nursing care of 12 patients with idiopathic scoliosis treated with halo-pelvic traction preoperatively which focused on breathing training, traction frame management. One patient suffered from temporary brachial plexus injury and four cases suffered from superior mesenteric artery syndrome. With 14-21 day's traction and nursing care, the correction rate of Cobb angle was 35%-50%,the forced vital capacity was improved by 25%,and all the patients received orthomorphia surgery in time. It is suggested that the patients with severe idiopathic scoliosis treated by halo-pelvic traction could take out-of-bed activity freely. It could not only relieve pain and reduce mental pressure, but also improve the safety of orthomorphia surgery.

Objective Vimentin is one of the proteins of cytoskele to n and cell movement. To investigate the effect of gene expression of fibroblast vimentin on the formation and contraction of hypertrophic scar in this report. Methods According to the results of differently expressed genes in hypertrophic scar by gene microarray, Vimentin, one of the most important g enes , was selected and made into oligonucleotide probe. 24 cases of hypertrophi c scar and 24 non-hypertrophic scar and 12 normal skin were used and these scar were taken on 3, 6, 9 and 12 months after burned. Frozen section and cultured f ibroblasts were made to detect the expression of the gene by in situ hybridizati on. Results Expression of vimentin was defected in scar tissue, but those in the hypertrophic scar on 3 and 6 months after burn were significan tly stronger than those in the hypertrophic scar on 9 and 12 months after burn a nd non-hypertrophic scar. Conclusions Over-expression of cyto skeletal relative genes causes the contraction of scar and vimentin acts leading and key functions.

Thyroid neoplasm is one of the most common endocrinal tumors. In general, thyroid cancer patients are usually presented with asymptomatic neck nodules. A differential diagnosis between malignant and benign thyroid neoplasm is very important for these patients. This review article primarily discusses biological markers used to aid the differential diagnosis of thyroid neoplasia, which may lead to a more rational approach to the treatment. It mainly describes the recent development of molecular biological markers VEGF-C, Cytokeratin-19, HBME-1, Pax8-PPAR and Galectin-3 for the differential diagnosis of thyroid neoplasms.

The Hippocratic oath commands doctors to be the patients supreme advocate. Coupling this command with a well trained physician is the sine qua non of orthopaedic risk management. A favorable doctor and patient relationship is also essential. The occurrence of a malpractice lawsuit is more often the result of the failure to practice the art of medicine by the orthopaedist rather than a failure to apply the science of orthopaedics. The ER (emergency room) is a dangerous area. The risk of litigation in the emergency room is not directly proportional to the severity of injury. In fact, the risk of initiation of legal action is greater in cases of relatively modest disability.