Objective To investigate the effect of Astragalus and chemotherapy on the expression of vascular endothelial growth factor(VEGF)in non-small cell lung cancer(NSCLC), microvessel density(MVD)and immune function.Methods 92 patients with NSCLC were divided into the study group and the control group, 46 cases in each group.Both groups were treated with neoadjuvant chemotherapy and operation, and the study group was treated with astragalus polysaccharide.The expression of VEGF in cancer tissues, MVD and immune function were compared between the two groups.The short-term and long-term curative effect and the incidence of adverse reactions were recorded.Results After treatment, positive rates of VEGF, MVD and blood CD8+ were significantly decreased in two groups (P<0.05), showing study groupcontrol group (P<0.05).There were no significant differences between the two groups in short-term curative effect and 5 year survival rates.The incidence rates of side effects in the study group were significantly lower than those in the control group (P<0.05).Conclusion Adjuvant chemotherapy combined with intravenous infusion of Astragalus Polysaccharide can improve MVD, the expression of VEGF and immune function in patients with NSCLC.

Objective: To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis. Methods: A total of 76 patients were selected and randomly divided into two groups by the random number table method, with 39 cases in the treatment group and 37 cases in the control group, respectively. Patients in both groups received routine anti-osteoporosis treatment. Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu (BL 20) and Shenshu (BL 23), while patients in the control group received additional intramuscular injection with salmon calcitonin. The treatments for both groups were given once a day and lasted for 4 weeks. Visual analog scale (VAS) and Chinese Oswestry disability index (CODI) scores were observed before treatment, after 2 weeks and 4 weeks of treatment, and the use of analgesics during the treatment were recorded. Results: After treatment, the clinical efficacy in the treatment group was more significant than that in the control group (P<0.05). After 2 weeks and 4 weeks of treatment, the VAS scores in both groups showed significant intra-group and between-group differences (all P<0.05), and the CODI scores in both groups showed significant intra-group differences (all P<0.05). After 2 weeks of treatment, the CODI score showed no significant between-group difference (P>0.05). After 4 weeks of treatment, the improvement of CODI score in the treatment group was more significant than that in the control group (P<0.05). During the treatment, 2 cases in the treatment group took analgesics versus 8 cases in the control group, and the result showed a significant between-group difference (P<0.05). Conclusion: For back pain in elderly patients with primary osteoporosis, based on the routine treatment of oral medication, the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu (BL 20) and Shenshu (BL 23) is more significant than that of intramuscular injection. Acupoint injection treatment can improve patients' conditions and reduce the use of analgesics.

This paper reports the nursing care of 12 patients with idiopathic scoliosis treated with halo-pelvic traction preoperatively which focused on breathing training, traction frame management. One patient suffered from temporary brachial plexus injury and four cases suffered from superior mesenteric artery syndrome. With 14-21 day's traction and nursing care, the correction rate of Cobb angle was 35%-50%,the forced vital capacity was improved by 25%,and all the patients received orthomorphia surgery in time. It is suggested that the patients with severe idiopathic scoliosis treated by halo-pelvic traction could take out-of-bed activity freely. It could not only relieve pain and reduce mental pressure, but also improve the safety of orthomorphia surgery.

Objective:To study the regulatory effect of somatostatin analogue octreotide on human gastric cancer cell line SGC7901 and to explore the corresponding mechanisms.Methods:Moderately differentiated human gastric carcinoma SGC-7901 cells were treated with octreotide in vitro.SGC-7901 cells treated with 5-FU were the positve controls and human fibroblasts were the normal controls.MTT assay was used to observe the inhibitory effect of octreotide on human gastric carcinoma cells and human fibroblasts.We observed the apoptosis through fluorescent microscope.The influence of octreotide on cell cycle distribution and the apoptosis rate of human gastric carcinoma cell were analyzed with FCM.Radiommunoassay was employed to determine the changes in IGF-1 levels in cell culture fluid.Results:Octretide can not inhibit the growth of gastric cells at low concentration(50ug/L).With the increase of octretide concentration,the inhibitory effect increased gradually,in a dose-dependent manner.Octretide had an evident inhibitory effect on human fibroblasts(P>0.05).There was no difference in the inhibition of SGC-7901 cell growth between octretide (500ug/L)and 5-FU(50mg/L)(P>0.05).At 48 hours after treatment with octretide(1 mg/L),the morphological changes of apoptosis were seen under fluorescent microscope.At 48 hours after treatment with octretide (500ug/L),most cells were blocked at G_0/G_1 phase(72.07±2.40).The percentage of cells at S phase was decreased signiflcantly(14.99±1.42).The proliferation of cells was inhibited and the apoptosis rate was increased(21.40±2.71).With octretide treatment at different concentrations.IGF-1 level in cell culture fluid was significantly lower than that in the control group(P<0.01),indicating that octretide down-regulated IGF-1 level in the call culture system.Conclusion:Octroetide can inhibit the growth of gastric carcinoma cells in vitro,with no significant inhibition on the growth of non-target cells.Octroetide can induce gastric cancer cell stagnation at G_0/G_1 phase and apoptosis,inhibiting the proliferation directly.Octroetide can also inhibit the secretion of IGF and restrain tumor cell growth indirectly.

AIM: To explore the role of Akt signal pathway in apoptosis of neural cells in adult rats treated with Zuogui Pill, a Chinese medicine. METHODS: Flowcytometry and Western blotting methods were used to investigate the changes of cellular apoptosis rate and Akt signal pathway. RESULTS: Monosodium glutamate (MSG) could increase cellular apoptosis rate and significantly restrained the phosphorylation of Akt (Ser473) and Akt (Thr308), and markedly increased the levels of phospho-FKHR (ser256), GSK-3? (Ser9) and PTEN. Zuogui Pill partly inhibited the above effects of MSG. CONCLUSION: Zuogui pill effectively inhibits the neural apoptosis induced by MSG, and Akt pathway is involved in the neuronal protection of Zuogui pill. [

AIM: To explore the changes of related proteins of neural stem cell differentiation and proliferation in rats treated with corticosterone (CORT). METHODS: Western blot method was used to investigate the changes of related protein of neural stem cell differentiation and proliferation. RESULTS: Compared with the normal control group, related proteins (Notch1, hes5, Mash1 and NeuroD) of neural stem cell differentiation and proliferation were significantly decreased in the CORT rats on the day 30 and 60, especially for Mash1 and NeuroD (P

BACKGROUND: Infusion of hemopoietic stem cell from donors can promote the chimeric formation and induce specific immunologic tolerance in the allograft recipients. However, the pretreatment for cell transplantation has great toxicity to recipients. So immunosuppressant combined bone marrow infusion is introduced to anti graft versus host reaction. OBJECTIVE: Based on microchimerism, to study the security and associativity of chimera formation induced by kidney-bone marrow transplantation and immunologic tolerance.DESIGN, TIME AND SETTING: The contrast observation was performed at the department of urinary surgery, The Third People's Hospital of Zhengzhou City from January 1998 to December 2005.PARTICIPANTS: According to ABO/Rh blood type and HLA matching, 96 female patients with chronic renal failure and waiting for kidney transplantation were divided into 2 groups, In the combination group, patients received kidney combined bone marrow transplantation; the other uremia patients received the other kidney of cadavers were served as control group. The donors were 48 healthy males. METHODS: Bone marrow of donors was collected simultaneously with kidney obtain and preserved with cryoprotectant at -198 ℃ in nitrogen canister. After kidney transplantation, large dose of anti-human lymphocyte immune globulin were used for 2 weeks, then (0.9-2.5)×10~8/kg mononuclearcell was reinfused. PCR-SRY was used to identify donor derived cell-chimerism. Lymphocyte subgroup of recipients was determined by blood test; and interleukin 10 was measured by enzyme linked immunosorbent assay; in addition, the mass concentration of tumor necrosis factor α and tumor necrosis factor β was detected. MAIN OUTCOME MEASURES: Chimerism, lymphocyte subsets and cytokines were detected at various time points following transplantation. Simultaneously, the transplantation results and complication status of recipients were observed. RESULTS: The positive rate of chimera in the combination group was greater than that of the control group (P < 0.05). The 3-year follow-up showed that incidence differences of acute rejection between recipients with positive chimera and recipients with negative chimera had significance (13%, 35%, P < 0.05). There was no graft versus host disease occurred in the combination group. CONCLUSION: Kidney-bone marrow transplantation can augment chimerism in early postoperative period, and significantly reduce the rate of acute rejection, which is safe and beneficia1to induce specific immunologic tolerance in the renal allograft recipients.

BACKGROUND:Sunshine time in northem region is short in winter,the infants and young children are vulnerable to lack of vitamin D. Up-to-date textbooks and the guidelines formulated by Chinese Medical Association account that glass block ultraviolet and indoor exposure to human is meaningless. Early studies have shown that sunlight exposure through glass had meaning in rats,but it was difficult to accurately quantify,while outdoor exposure in rats is difficult to continue. This expedment uses B-band ultraviolet rays,which has the impact on vitamin D metabolism in some wavelengths,to facilitate further study.OBJECTIVE:To study the effect of ultraviolet B exposure in laboratory on 25-hydroxy vitamin D(25-OHD)level and bone metabolism in the serum of growing rats.DESIGN,TIME AND SETTING:A randomized controlled animal experiment was performed in June 2007 in the ExperimantalAnimal Center of Chinese PLA General Hospital.MATERIALS:Forty male 21-day-old Wistar rats were employed in this study. Ultraviolet waves were sourced from an artificial UV light instrument(wavelength 280-350 μm,irradiation intensity 5.5μW/cm~2).METHODS:Forty Wistar rats were divided into four groups by random sampling table,exposed to different doses of ultraviolet Bwave. Direct exposure group:direct exposure for 20 minutes at an irradiation dose of 4.2 mJ/(cm~2·d);Indirect exposure 60 and 120 min groups:exposure through a single pane of glass for 60 and 120 minutes at an irradiation dose of 0.36 and 0.72 mJ/(cm~2·d).Control group:no exposure was given. For the direct exposure group,the lamp was placed above the cage. A 3-mm-thick pane of glass(common window glass) was placed underneath the lamp in the indirect exposure groups. Exposure groups were given irradiation for successive 20 days.MAIN OUTCOME MEASURES:Serum 25-OHD,bone alkaline phosphatase (BALP),and bone mineral density (BMD) were measured on day 21.RESULTS:25-OHD was significantly higher in all exposure groups compared with the control group (P＜0.001),but there was no difference between the direct exposure group and two indirect exposure groups. BALP was significantly lower in the exposure groups than control group (P＜0.001),it was also significantly lower in the 120-min indirect group than in the 60-min indirect group (P=0.022). There was a positive correlation between exposure dose and 25-OHD (r=0.555,P=0.002) and a negative correlation between exposure dose and BALP (r=0.595,P=0.001),also a negative correlation between 25-OHD and BALP (r=0.569,P=0.002),but there were no differences between groups for BMD. Exposure dose exhibited a threshold,serum 25-OHD and BALP no longer increased or decreased when it was 0.36 mJ/(cm~2·d).CONCLUSION:Midwave ultraviolet rays might affect serum 25-OHD and BALP levels in the growing rats through glass exposure,with no significant difference compared to direct exposure. The B-band ultraviolet exposure dose may play an important role in serum 25-OHD synthesis,but there is a threshold dose for the synthesis. Low-dose and prolonged exposure time also achieve threshold exposure.

Objective To observe the clinical effect and safety of percutaneous kyphoplasty (PKP) on osteoporosis vertebral compres-sion fractures (OVCF) in elderly. Methods From January, 2014 to May, 2015, 88 patients with OVCF treated with PKP were retrospective studied. Visual Analogue Scale (VAS) and Oswestry Dysfunction Index (ODI) were used to evaluate the pain and quality of life, and the ky-photic angle (Cobb's angle) and anterior vertebral body height were recorded before operation, one week after operation and the last fol-low-up. The cement leakage and its effect on nerve function were recorded. Results Bone cement leakage was found in eleven patients, in which two in pedicle, three in venous leakage, three in intervertebral disc and three in vertebral posterior. Nerve root symptoms appeared in one patient, who was treated with decompression. The scores of VAS and ODI, and the Cobb's angel were significantly lower (t>29.518, P<0.001), and the vertebral body height was significantly higher (t>35.173, P<0.001) one week after operation and the last follow-up than be-fore operation. Conclusion PKP is safe and effective for OVCF in elderly. It can efficiently reduce the pain, correct kyphotic deformity and improve the quality of life.

Objective To investigate radiation-related human plasma metabolic features by using metabonomics method and to analyze relative metabolic pathway .Methods The plasma samples of 40 patients pre-and post-total body irradiation (TBI) from January 2012 to May 2014 were collected, and the effect of TBI on human plasma metabolites was studied by gas chromatography mass spectrometry ( GC-MS) , and the differential plasma metabolic features related to irradiation damage were screened . Results The levels of glucose, myristic acid, oxalic acid, 3-hydroxy butyric acid, urea, aspartic acid, valine, leucine, lysine and threonine in plasma were significantly (P<0.05) increased after TBI, while the levels of cholesterol, pyruvic acid, propionic acid, lactic acid, alanine, glycine, inositol, sorbitan, ethylene glycol and hypoxanthine were decreased drastically (P<0.05).Conclusions TBI could cause significant changes in the levels of human plasma metabolites including amino acid metabolism , glucose metabolism, lipid metabolism and so on.