Human metapneumovirus (HMPV)is a recently discovered virus associated with respiratory tract infection. Most infections with HMPV occurred during winter and spring. Children, elderly subjects and immunosuppressed hostsare susceptible. The consequences of HMPV infections ranged from mild upper respiratory tract infection to severe bronchiolitis or pneumonia. There are three different kinds of methods for the diagnosis of HMPV infections, detection of virus antigen-antibody, virus isolation by culture, and RNA detection by reverse transcription-PCR. Symptomatic therapy is the main method of treatment.

Hemophagocytic lymphohistiocytosis (HLH) is a syndrome characterized by extreme immune activation, resulting in pathologic inflammation. The HLH-2004 diagnostic criteria is used widely based on the data from pediatric patients. However, there are differences between children and adults in the etiology, clinical and laboratory characteristics for HLH. Given these differences, diagnostic scoring systems unique to adult HLH have been proposed. HLH in adults is a much more heterogeneous syndrome requiring a more individualized approach depending on the underlying trigger, disease severity, as well as genetic background.

Objective To investigate the influence of Fasudil on oxidative stress in patients with early-stage diabetic nephropathy. Methods 48 patients with earl-stage diabetic nephopathy were randomly divided into control group treated with Alpha-lipoic acid and treametn group with Fasudil. All patients were under strict control of blood glucose. Changes in the levels of serum cysc, urinal mALB and 8-OHdG were observed after two weeks treatment were compared between the two groups. Results Compared to pre-treatment, the content of serum cysc, urinal mALB and 8-OHdG were significantly decreased in both groups after treatment. There was no significant difference in the content of urine mAlb , CTGF and MCP-1 between the two groups before and after treatment (P > 0.05). Conclusion Fasudil may protect kidney through a mechanism by which the level of 8-OHdG in kidney is decreased and oxidative stress is alleviated.

Objective To investigate the inter-arm invasive blood pressure differences during hip replacement in patients with lateral position and its relationship with position.Methods Sixty-six patients having underwent hip replacement in orthopedic surgery with lateral position were enrolled.Invasive blood pressure measurements were obtained from bilateral radial artery in all patients every 5 minutes.Simultaneously bilateral invasive blood pressure measurements were obtained and compared.Results At supine position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (128.16 ±20.09),(69.47 ± 8.64) and (89.03 ± 10.42) mmHg (1 mmHg =0.133 kPa);systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (127.90 ± 20.44),(69.86 ± 8.81) and (89.21 ± 10.71) mmHg.There was statistical difference in inter-arm diastolic pressure (P ＜ 0.05),but inter-arm systolic pressure and mean arterial pressure had no statistical differences (P ＞ 0.05).At left-lateral position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (129.12 ± 21.93),(69.23 ± 8.92) and (89.19 ± 11.35) mmHg;systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (129.01 ± 21.72),(69.51 ± 9.02) and (89.34 ± 11.35) mmHg.There was statistical difference in inter-arm diastolic pressure (P ＜ 0.05),but inter-arm systolic pressure and mean arterial pressure had no statistical differences (P ＞ 0.05).At right-lateral position,systolic pressure,diastolic pressure and mean arterial pressure of the uninjured side were (128.78 ± 20.34),(69.54 ± 9.06) and (89.29 ± 10.82) mmHg;systolic pressure,diastolic pressure and mean arterial pressure of the injured side were (128.27 ± 20.56),(70.00 ± 9.09) and (89.43 ± 10.89) mmHg.There were statistical differences in inter-arm systolic pressure and diastolic pressure (P ＜ 0.05),but inter-arm mean arterial pressure had no statistical difference (P ＞ 0.05).The bilateral radial artery blood pressure difference under different positions was ＜ 1 mmHg,and had no clinical practical significance.There was no correlation between blood pressure and age,sex,body mass index.Conclusion Bilateral radial artery in the same patients with invasive blood pressure has no clinical difference,and patients with lateral position operation for radial artery puncture pressure need not consider the differences of blood pressure between arms.

Objective To induce and analysis the death relative factors of peritoneal dialysis patients over the past five years in fourth ward of qiandongnan from guizhou province guiyang medical college affiliated people's hospital in endocrinology and to improve the effect of nursing intervention.Methods 492 peritoneal dialysis patients were retrospective analyzed from Mar 2009 to Mar 2014,in order to conclude the causes of the patients that ending with death (n =89) and to formulate targeted nursing measures.Results The primary disease for death of maintained peritoneal patients was cardiovascular events [41.6％(37/89)],and the second factor was infection [32.6％(29/89)].The risk factors for mortality were age (≥70 years old),diabetes,endogenous creinine clearance rate declined [＜50 L·week-1 ·m-2],change of hemodialysis to peritoneal dialysis and higher peritoneal transport status.Conclusions It's important to strengthen the nursing intervention for the peritoneal dialysis patients with coronary heart disease,and to formulate targeted nursing measures for risk factors,so as to avoid the death.

OBJECTIVE:To observe the efficacy and safety of Compound sabal berry tablet combined with tamsulosin in the treatment of benign prostatic hyperplasia complicated with urinary obstruction. METHODS:86 patients with benign prostatic hyper-plasia complicated with urinary obstruction were randomly divided into control group(43 cases)and observation group(43 cases). Control group orally received 0.2 mg Tamsulosin hydrochloride orally disintegrating sustained-release tablet,once every evening. Observation group additionally received 500 mg Compound sabal berry tablet before a meal,3 times a day. 4-week was regarded as 1 treatment course,and it lasted 3 courses. During the treatment,all patients in the two groups ate light food,avoiding spicy and bland food. The clinical efficacy,TCM symptom scores,interleukin-8 (IL-8),interleukin-10 (IL-10),tumor necrosis factor α(TNF-α),maximum flow rate(Qmax),post-void residual(PVR),the International Prostate Symptom Score(IPSS)scores before and after treatment and the incidence of adverse reactions in the 2 groups were observed. RESULTS:The total effective rate in ob-servation group was significantly higher than control group,the difference was statistically significant(P<0.05). Before treatment, there were no significant differences in the TCM symptom scores,Qmax,PVR,IPSS scores,IL-8,IL-10 and TNF-α between 2 groups (P>0.05). After treatment,TCM symptom scores,IPSS scores,IL-8,IL-10,TNF-α and PVR in 2 groups were signifi-cantly lower than before,and observation group was lower than control group,Qmax was significantly higher than before,and obser-vation group was higher than control group,the differences were statistically significant (P<0.05). And there were no severe ad-verse reactions during treatment. CONCLUSIONS:The efficacy of Compound sabal berry tablet combined with tamsulosin is superi-or to tamsulosin alone in the treatment of benign prostatic hyperplasia complicated with urinary obstruction,it can reduce the in-flammatory reactions and residual urine,with good safety.

Cholelithiasis has still been a common and endemic disease damaging people's health in our country.Management modes and prognosis of this disease have been changed and improved greatly in the past fifty years.Based on our own clinical practice and experience in the People's Hospital of Hunan Province,we reviewed aspects concerning on diagnosis,principles and technical considerations of surgical treatment,summarized background and changes in the past almost half century,which reflected our unremitting efforts and distinctive contributions in management of cholelithiasis.Our experience demonstrated that cholelithiasis should not be considered as a gradually disappearing disease,difficulties and perplexities in dealing with such disease would still be arduous challenges for surgeons,although rates of stone residue and reoperation decreased dramatically.

Objective To understand the epidemic situation,epidemiological characteristics and clinical features of import?ed malaria in Donghai County,Lianyungang City from 2005 to 2015. Methods The epidemiological data of imported malaria in Donghai County from 2005 to 2015 were collected and analyzed by the descriptive epidemiological method. Results A total of 126 imported malaria cases were reported in Donghai County from 2005 to 2015,including 101 falciparum malaria cases (80.19%),13 ovale malaria cases(10.32%),7 vivax malaria cases(5.56%),4 quartan malaria cases(3.17%),and 1 case with mixed infection(0.79%). These cases were distributed in 14 towns,and mainly in 4 towns namely Niushan,Tuofeng,Shil?iu,and Huangchuan,and the cases in the 4 towns accounted for 63.49% of the total cases. The cases occurred in the whole year,but the peak period of the disease was in April,which was related to the returned time of these patients from foreign coun?tries. All the patients were male and aged from 20 to 55 years,and those aged from 20 to 50 years accounted for 90.70%. Con?clusions The epidemic situation of imported malaria presents a rising trend in Donghai County in recent 10 years. To control imported malaria,the above?mentioned 4 towns with more migrant construction workers and businessmen should be treated as key areas. Meanwhile,the floating population management,disposal of the malaria epidemic spots,mosquito vector monitoring as well as health education should be strengthened.

OBJECTIVE:To explore the effects of pioglitazone on secreation and mRNA expressions of TNF-? in adipocytes of high-cholesterol model rats.METHODS:30 rats fed with high-cholesterol diet for 8 weeks were randomly assigned to receive high cholesterol diet alone(high-cholesterol group,n=15) or high-cholesterol diet plus pioglitazone(3 mg?kg-1?d-1) (pioglitazone group,n=15) for 4 weeks.Another 15 rats were assigned to control group and fed with common diet for 12 weeks.Then the rats were sacrificed,with indexes such as blood lipid,serum concentration and mRNA expression of TNF-? in adipocytes of high-cholesterol rats determined.Besides,normal adipocytes were taken and treated with lipopolysaccharide and different concentrations of pioglitazone (0.1,1.0,10.0 ?mol?L-1) followed by a determination of TNF-? level and mRNA expression level.RESULTS:Compared with high-cholesterol group,serum concentration and mRNA expression of TNF-? in pioglitazone group were significantly lowered,but pioglitazone treatment showed little impact on blood sugar level and blood lipid level.Pioglitazone(0.1,1.0,10.0 ?mol?L-1) dose-dependently inhibited lipopolysaccharide-induced TNF-? secretion and mRNA expression in cultured adipocytes.CONCLUSION:Pioglitazone can reduce TNF-? level of fatty tissue and serum in high-cholesterol model rats.

This paper reviews the research progress in relations between indoor air pollution and human health for the last 20 years,and focuses on: the studies on environmental risk assessment methods; the major health effects caused by indoor air pollution; the minor and limited health effects caused by indoor air pollution; other key issues concerned.