Long-term use of nucleoside ( nucleotide ) analogues for anti-HBV treatment care easily lead to drug resistance. As compared to genotypic resistance detection, the detection technology of phenotypic resistance is a method used to analysis the sensitivity of antiviral drugs in vitro cell model, the result can directly reflect the relationship between drug-resistance mutations and drug susceptibility, thus perform a better prediction of clinical resistance.Among those technologies, some analysis strategies like transient transfection assay, stable cell lines and enzymatic assay, have been developed for early diagnoses as well as dynamic monitoring of drug resistance, and contribute greatly to proper selection of therapeutic regimen, reduction of resistance rate and implementation of individual treatment.

Graduation practice of clinical laboratory profession is the important link of the teaching.In this stage,it is necessary for the students to adapt to the transition role and the thought method,and deal with interpersonal relation,cultivate noble professional ethics,master skills as soon as possible and get aware of the protection of the safe medical treatment to avoid the medical entanglement.It is also very important to accelerate personal skill,increase practice result and cultivate talented persons.

4-Hydroxycoumarins ; poisoning ; Child ; Female ; Humans ; Lower Extremity ; blood supply ; Thrombosis ; etiology

Hypertension is a common chronic disease which can lead to cardiac, cerebrovascular and renal damage; it is seriously harmful to human health. Cardiovascular disease caused by hypertension has become the first cause of death of residents in our country, the burden of disease has become more and more serious, how to manage hypertension has become an important public health issue in our country. In this review, the development and current situation of the community health management of hypertension at home and abroad are described and analyzed, the experience and practice worthy of learning are summarized to provide evidence for the prevention and control of hypertension in community.

AIM: To study the effects of guava polysaccharides on blood glucose level and antioxidant activity in alloxan-induced diabetic mice. METHODS: The animal model of diabetes was established by injecting alloxan into mice abdominal cavity.The mice were divided into five groups: normal control group,diabetic model group,guava polysaccharides group I,guava polysaccharides group II,glybenclamide group.Blood glucose level was determined with blood glucose monitor 3 d and 10 d after being given polysaccharides.The mice were anaesthetized and killed on the tenth day.Activity of SOD and concentration of MDA in blood serum and liver were determined. RESULTS: Compared with diabetic model group,the blood glucose level of guava polysaccharides group I and guava polysaccharides group II significantly decreased.Activity of T-SOD in blood serum and liver significantly increased,while concentration of MDA in blood serum and liver significantly decreased. CONCLUSION: Guava polysaccharides can significantly decrease blood glucose level and improve the antioxidant activity of diabetic mice.lt is a potential hypoglycemic agent.

OBJECTIVETo compare the effect of three cannulated screws and dynamic hip screw (DHS) combined with antirotation screw in treating patients with comminuted fractures of femoral neck.

METHODSFrom January 2008 to December 2012,67 patients with comminuted fractures of femoral neck were treated with three cannulated screws or DHS combined with antirotation screw. Among them, 36 patients were treated with DHS combined with antirotation screw including 24 males and 12 females with an average of 43.7 years old ranging from 22 to 58 years old; 31 patients were treated with three cannulated screws including 22 males and 9 females with an average of 43.0 years old ranging from 24 to 56 years old. The incision size, operation time, blood loss, blood transfusion rate, postoperative hospital stay, walking time, weight-bearing time, union time, incidence of complication were recorded and compared between two groups, and functional outcomes were evaluated by Harris scoring.

RESULTSAll incision healed at stage I. The differences in operation time, blood loss, blood transfusion rate and postoperative hospital stay between two groups were not significant (P > 0.05). Incision size of DHS group (5.00 ± 0.86) cm was larger than that of cannulated screw group (3.30 ± 0.57) cm (P < 0.01). All patients were followed up more than one year, and the follow-up time showed no significant difference between two groups (P > 0.05). Walking time of DHS group (19.0 ± 3.5) d was less than that of cannulated screw group (37.0 ± 6.3) d (P < 0.01. Partial and full weight-bearing time of DHS group (23.0 ± 7.0) d and (138.0 ± 13.0) d was less than that of cannulated screw group (38.0 ± 5.7) d and (164.0 ± 12.0) d (P < 0.01). Union time of DHS group (151 ± 11) d was less than that of cannulated screw group (162 ± 11) d (P < 0.01). Harris hip score of DHS group (91.0 ± 5.7) was higher than that of cannulated screw group (85.0 ± 12.0) (P < 0.01). No significant differences were found in the incidence of postoperative complications between two groups (P > 0.05).

CONCLUSIONThe DHS combined with antirotation screw is superior to three cannulated screws for treatment of comminuted fractures of femoral neck due to earlier weight-bearing, faster fracture healing and better hip function

Adult ; Bone Screws ; Female ; Femoral Neck Fractures ; surgery ; Follow-Up Studies ; Fracture Fixation, Internal ; adverse effects ; methods ; Fractures, Comminuted ; surgery ; Humans ; Male ; Middle Aged

Objectve To probe the effect of treating the trauma soft tissue defects of the leg by microsurgical technique. Methods Ninety-nine cases of the trauma soft tissue defects of leg were treated by used 12 kinds of microsurgical flaps.Result All cases with soft tissue defects of the leg were repaired successfully.Follow-up 6 months to 10 years,the results were satisfactory and function improvement in 99 cases. Conclusions Accoding to soft tissue defects of size,the section and the technique of the operator to select the different microsurgical flap could be suitable and resolvable problem of soft tissue defects.

Objective To evaluate the efficacy and safety of interferon alpha (IFNα) combined with adefovir dipivoxil (ADV) for patients with chronic hepatitis B (CHB) non-responding to 24-week IFNα monotherapy.Methods Sixty CHB patients admitted to the First Affiliated Hospital of Xiamen University during 2009 and 2012 were enrolled in the study.All patients recieved IFNα monotherapy for 24 weeks and had no response.The patients were randomly divided into 3 groups by number table with 20 cases in each group.The experimental group was treated with IFNα combined with ADV,the control group 1 continued IFNα monotherapy,and the control group 2 shifted to ADV monotherapy.Virological,serological and biochemical responses were compared,and adverse reactions were observed.SPSS 19.0 software was used for data analysis.Results After 24 weeks of treatment,there was no statistical difference in HBV DNA loads,ALT levels and titers of HBeAg and HBsAg among three groups (F =0.985,0.717,0.985 and 0.717,P ＞ 0.05).And no HBeAg seroconversion was observed.After 48 weeks of treatment,the experimental group had higher HBV DNA negative conversion rate,ALT normalization rate and HBeAg conversion rate than control group 1 (x2 =10.00,3.956 and 4.800,P ＜ 0.05),but no statistically significant difference was found in HBeAg negative conversion rate (x2 =0.693,P ＞ 0.05).There was no significant difference in HBV DNA and HBeAg negative conversion rates between experimental group and the control group 2 (x2 =1.026,1.905 and 0.156,P ＞0.05),but the HBeAg conversion rate in experimental group was significantly higher than that in control group 2 (x2 =4.800,P ＜ 0.05).No HBsAg negative or serological conversion was observed,and there was no significant difference in titers of HBsAg among three groups (F =1.935,P ＞ 0.05).No adverse reaction was observed.Conclusion For patients nonresponding to IFNα monotherapy,combination of IFNα and ADV can achieve higher ALT normalization rate,HBeAg conversion rate and HBV DNA negative conversion rate,and improve the overall efficacy of CHB antiviral therapy.

Wuhutang, a well known traditional Chinese. herbal decoction for the treatment of as thma was found to havc an antiviral effect on respiratory syncytial virus (RSV ) both invitro and in vivo. A 50% reduction of plaque. formation was observed op Hep-2 cell monolayer at a concentration of 1:80. At such concentration, the. value of lg TC ID50 of. Viralyield was 2, 1, and 55% RNA synthesis of RSV was inhibited. It was found that among thefive medicinal herbs in prescription Ephedra sinica Stapf is responsible for the antiviralactivity on RSV. IC50 for plaque inhibition was found to be 1.6mg/ml. In animal model,Wuhutang singn ficantly reduced the titre of RSV and accelerated RSV clearance in mice,and alleviated viral pneumonitis by either oral or aerosol route.

Purpose:To study the effect of NVB in the treatment of advanced non small cell lung cancer (NSCLC).Methods:In 39 cases advanced stage, primary chemotherapy patients account for 30 cases and 9 were recurrent diseases.Results:CR 2 cases PR 20 cases NC 13 cases and PD 4 cases, total response rate 56.4%. The majory toxicity was bone marrow suppression.Conclusions:Combined chemotherapy including NVB has better curative effect for advanced NSCLC.