China ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Objective To explore the therapeutic effect of loop electrosurgical excision procedure (LEEP)in the treatment of patients with cervical intraepithelial neoplasia (CIN) and its influence on pregnancy outcome.Methods 62 women with CIN who underwent LEEP were selected as treatment group,and 63 women with CIN who did not undergo LEEP in the same period were selected as control group.Then the curative effect and the pregnancy outcome were observed.Results The total effective rate was 93.65％ in the treatment group and 76.19％ in the control group,the difference was significant (x2 =7.034,P ＜ 0.01).The operation blood loss,operation time and the day of colporrhagia after operation in the treatment group were less than those in the control group(all P ＜ 0.05).There were no significant differences in the gestational age,incidence rates of abortion and cesarean section between the two groups (all P ＞ 0.05),while there were significant difference in the incidences of preterm delivery,premature rupture of membrane and low birth weight infant (all P ＜ 0.05).Conclusion LEEP is an effective treatment for patients with CIN,which can increase the incidence rates of preterm delivery,premature rupture of membrane and low birth weight infant,but not increase the gestational age,incidence rates of abortion and cesarean section.

A patient with sparganosis mansoni was treated with praziquantel and cured with little side effect.

A patient with eosinophilic syndrome due to Paragonimus infection wag treated with praziquantel and cured.

Objective To investigate the activation of matrix metalloproteinase (MMP)9 by acute arterial injury and the involved signaling mechanism in murine femoral artery. Methods In the C57BL/6 mice femoral artery denudation injury were performed. Total protein and membrane protein extracts were prepared from targeted arteries. The MMP 9 activity was measured by zymography assay, the expressions of MMP 9 antigen and protein kinase C (PKC) isoforms were measured by Western blot. Seventy-two hours after mice fed with PKCβ inhibitor (ruboxistaurin), the denudation injury triggered MMP 9 activation was reassessed. Results Within 4-24 hours after denudation injury, MMP 9 activity in femoral arteries was significantly increased, with a peak induction of (99.3±9.5) times the sham control (F=51.49,P＜0.01) at 8 h. MMP 9 antigen increased in parallel with MMP 9 activity. Within 15-120 minutes after denudation injury, there was a significant induction of PKCβⅡ in membrane fraction of femoral arteries, with a maximum induction of (7.50±0.60) times the sham control (F=207.06,P＜0.01)at 30 min. Injury-induced MMP 9 activation was significantly inhibited by ruboxistaurin. Conclusions MMP 9 activation is, at least in part, mediated by PKCβ in acute arterial denudation injury, it highlights the new target for therapeutic intervention to suppress the over-activation of MMP 9, which plays a critical role in restenosis.

ObjectiveTo explore the effective measures to prevent and control the hospital infections.MethodsThe hospital infections'data of 27 129 inpatients were retrospectively studied and retrospectively analyzed.ResultsThe nosocomial infection rate was 3.40％. The major sites of the infection were lower respiratory tract (50.05％ ). The most common pathogens were mainly Gram-negative bacteria (41.91％ ). The infection mainly occurs in old peple(75.34％ ). The highest infection rate is in organ transplantation unit( 14.62％ ). The factors of high risk included antibiotics(53.47％ ), immunocompromise and invasive therapies. ConclusionTo improve the pathogen detective rate and the monitoring of antibiotic resistance ,to decrease the use of invasive therapies and to keep high risk areas under surveillance were the effective measures to control hospital infection and to reduce the incidence rate of hospital infection.

Objective To clarify the morphological features, types, regional distributions and cell densities of gastrointestinal (GI) endocrine cells in various parts of the digestive track (DT) of Eumeces elegans. Methods Using immunohistochemical techniques of streptavidin-peroxidase(S-P) method. Results The endocrine cells found in the DT were round or oval, spindle, shuttle, pyramid, flask and bacilliform in shape. Three types of endocrine cells, namely 5-hydroxytryptamine (5-HT) positive cells, somatostatin (SS) positive cells and gastrin (GAS) positive cells were found. No substance P(SP)-, glucagon (GLU)-, pancreatic polypeptide ( PP)- and insulin(INS) positive cells were detected. 5-HT positive cells distributed throughout the whole DT at various densities and they were most predominant cell types in the DT. SS positive cells were detected only in stomach. GAS positive cells showed a restricted distribution and were only demonstrated in the pylorus and duodenum. In the DT of E. elegans the region with the highest degree of cell type heterogeneity was pylorus and all types of endocrine cell along the DT showed peak density in the pylorus as well. Conclusion Some common features of the distribution of different types of GI endocrine cells are found between E. elegans and other reptiles. This common trait may reflect the similarity in digestive physiology of various vertebrates. On the other hand, some species-dependent unique distribution features of endocrine cells in DT were also detected in E. elegans.

Objective To study the effects of repetitive transcranial magnetic stimulation (rTMS) on rats'locomotor function after hemisectional spinal cord injury (SCI). Methods A model of hemisectional SCI was established at the T10 segment in 12 rats,which were then randomly divided into an SCI-rTMS group and an SCI-control group.Another 6 rats served as normal controls.The SCI-rTMS group received threshold rTMS daily for 4 weeks,while the SCI-control group was given sham rTMS.The normal control group was left without any treatment.BassoBeatti-Bresnahan (BBB) locomotor scores were recorded weekly.Motor evoked potential (MEP) was detected at the 28th day after the operation.Neurofilament-200 (NF-200) was detected with immunofluorescent staining of the spinal cord lesions. Results The BBB scores in the SCI-rTMS group improved more than those in the SCI-control group.MEP of the fight hindlimb was detected in the rTMS group,but not in the control group.Expression of NF-200 marker increased significantly more than in the rTMS group. Conclusions rTMS can improve locomotor function of rats after spinal cord hemisectioning,which may result from increased expression of NF-200 and the regeneration of axons.

[Objective]This study explores the acknowledgement of Ma Zhaosheng ’s life. [Method]This study, having been conducted by searching and combing historical documents, might come to a conclusion. [Result]Ma Zhaosheng, whose style name was ruibo and whose pseudonyms were wujingandyudan Taoist, lived in Changshu of Jiangsu provine during the Ming dynasty. His birth and death years were roughly 1570-1650 years. Ma yuanjun was his father, brother Ma Zhaozhen. Ma Zhaosheng had four sons: Ma Jue with style name of boxian, Ma Cha with style name of zhongzhao, Ma Menggui with style name of qiuqing and Ma Tinggui with style name of dangu. The medicine works of Ma Zhaosheng, the last disciple of Miao Xiyong, were more than ten kinds, but most scattered, the only remaining were the book of The Revised Print Of Traditional Chinese Medicine and the article of The Foreword Of Opinions About Talking Medicine. Ma Zhaosheng paid more attention to herbalism, and had rich clinical experience and abundant writings. [Conclution]The textual research on Ma Zhaosheng provides an important historical for analysis of his medical achievements. This research facilitates the study of Miao Xiyong’s mentoring relationship, his medical ideas and clinical experience in depth.

Objective To investigate the relationship between serum hepatitis B virus markers (HBV-M ) and HBV-DNA . Methods Enhanced chemiluminescence enzyme immunoassay (ECLIA ) and real-time fluorescent quantitative polymerase chain re-action(FQ-PCR) were employed to detect HBV-M and HBV-DNA in 262 serum samples ,respectively .HBV surface antigen(HB-sAg) ,anti-HBV surface antibody(HBsAb) ,HBV e antigen(HBeAg) ,anti-HBV e antibody(HBeAb) ,anti-HBV core antibody(HB-cAb) were included in HBV-M .Results Compared the positive rates of HBV-DNA ,HBsAg ,HBeAb in HBeAg-negative patients with those in HBeAg-positive patients ,the differences were statistically significant (P< 0 .01) .29(36 .7% ) patients with HBV-DNA logarithm value not less than 5 were found in HBeAg-negative patients .Differences of HBeAg ,HBeAb positive rates among patients with different ages were statistically significant (P<0 .01) .25 patients with HBsAg less than 250 IU/mL were found in HBV-DNA-positive patients ,12(48 .0% ) of which showed HBV-DNA logarithm value not less than 5 .HBV-DNA logarithm value of HBV-DNA-positive patients was positively correlated to HBeAg and HBeAb (r= 0 .542 ,0 .607 ,P< 0 .01) .Conclusion Com-bined detection of HBV-M and HBV-DNA contributes to estimating the HBV infection .