BACKGROUND: The erythemal response of the skin to UVB radiation is used as a diagnostic phototest and guideline to phototherapy. OBJECTIVE: The purpose of this study was to compare the UVB-induced MEDs to the back, arm, and thigh skin METHODS: A sunlight fluorescenct lamp(Waldmann UV 7001K) was used as a UVB radiation source. The back, arm, and thigh skin were irradiated with the dose, from 40mJ/cm2 to 180mJ/cm2. The minimal doses for erythema responses to the skin were assessed visually at 24 hours after irradiation. RESULTS: MEDs of the back, arm and thigh skin were 92.6 +/- 17.3mJ/cm2(mean +/- S.D.), 123.0 +/- 24.2mJ/cm2, and 126.6+/- 28.3mJ/cm2, respectively. The most frequent MED was 100mJ/cm2 for the back skin and 120mJ/cm' for the arm skin and thigh skin. CONCLUSION: In this study, UVB-induced MEDs to the back, arm, and thigh skin in young adult Koreans were assessed. A significant difference in the MED was found between the back and extremities skin, with a lower value for the back skin(92.6 +/- 17.3mJ/cm2) than for the arm skin(123.0 +/- 24.2mJ/cm2) or for the thigh skin(126.6 +/- 28.3mJ/cm2).
Arm ; Erythema* ; Extremities* ; Humans ; Phototherapy ; Skin* ; Sunlight ; Thigh ; Young Adult*

BACKGROUND: Desmosomes are adhesive intercellular junctions that form an important component of the junction complexes of epithelial cells. They provide intercellular links between the intermediate filament cytoskeletons of adjacent cells and are thus involved in maintaining the structural integrity of tissues. OBJECTIVE: Calcium and retinoids are major regulators of epidermal differentiation and their role on keratin proteins are well known. However, their effects on desmosome moleucles are unknown. To address this question we initiated a study of the effects of these epidermal differentiation regulators on desmosomal components, i.e., desmoplakin, desmoglein, and pemphigus antigens. METHODS: We used monoclonal antibodies against desmoplakin(DP) and desmoglein(DG), and sera from patients with pemphigus vulgaris(PV), pemphigus foliaceus(PF) and paraneoplastic pemphigus (PNP) to study the effects of calcium and retinoic acids, which are major regulators of epidermal differentation, on desmosomal protein formation in human cultured deratinocytes. We performed immunofluorescence, immunoblotting and immunoprecipitation study using human keratinocytes cultured in high calcium media with or without retinoic acid and in low calcium media with or without retinoic acid. RESULTS: 1. In low calcium (0.15mM) media, PV antigen and DG were produced in a small amount and it appeared that these desmosomal proteins were located in cytosol. Whereas in high calcium (1.8mM) media, production of these desmosomal proteins was increased not they were assembled at the desmosomal structures located in cell-cell contact margins. 2. PF antigen, which was identical to the DG, were not produced or expressed in cultured keratinocytes even when cultured in high calcium media. 3. PNP antigen and DP were produced in cultured keratinocytes grown in both high low calcium media but their production was increased in high calcium media and only in high calcium media they were assembled at the desmosomal structures. 4. Retinoic acids induced loosening of cell-cell contacts of cultured keratinocytes and decreased the production of desmosomal proteins. CONCLUSION: Our results suggests calcium is a major regulator of the production and assembly of desmosomal proteins including pemphigus antigens, but PF sera and monoclonal antibodies against DG show different antigen binding characteristics. It appears that retinoic acids inhibit production of desmosomal proteins.
Adhesives ; Antibodies, Monoclonal ; Calcium* ; Cytoskeleton ; Cytosol ; Desmogleins ; Desmoplakins ; Desmosomes* ; Epithelial Cells ; Fluorescent Antibody Technique ; Humans ; Immunoblotting ; Immunoprecipitation ; Intercellular Junctions ; Intermediate Filaments ; Keratinocytes ; Pemphigus ; Retinoids ; Tretinoin*

The tibia is the most commoniy fractured of all the long bones of the body. Recently, the incidence of shaft fractured of tibia has risen as a result of rapid increase in automobile accidents, industrial accidents and other sports injuries. Fracture of the tibial shaft is extremely difficult to treat and a greater incidence of osteomyelitis, delayed union and nonunion of bone than in those of the full length of the tibia surface is throat, open fracture is more frequent in this bone than in any other major bones. Two hundred eighty patients (293 cases) of the fracture of tibial shaft were treated and managed at the Department of Orthopedic Surgery, Hanyang University Hospital from May, 1975 to December, 1977. The results were as follows: 1. The rate of fracture union was accelarated under the age of 20 years but it was slow in aged group. 2. A better prognosis (fracture union) was shown in proximal one third of the tibia than middle one third and lower one third of the tibia. 3. According to the classification of Ellis, the higher delayed and nonunion rate was shown in major severity group than moderate and minor severity group. 4. A better prognosis of the fracture type was shown in the spiral and oblique fracture than in the transverse, comminuted and segmental fractures. 5. Open fracture of the tibia united later than closed fracture, especially in positive culture sensitivity test. 6. There was a prolonged rate of union about 2 weeks in cases of associated fibula fracture. 7. The proper time of weight bearing of the shaft fracture of the tibia was helpful in fracture union. 8. A better prognosis was shown in the non-operative treatment than operative treatment, especially in PTB cast after long leg cast.
Accidents, Occupational ; Athletic Injuries ; Automobiles ; Classification ; Clinical Study ; Fibula ; Fractures, Closed ; Fractures, Open ; Humans ; Incidence ; Leg ; Orthopedics ; Osteomyelitis ; Pharynx ; Prognosis ; Tibia ; Weight-Bearing

The main purpose of this study was to identify the effects of physical therapy modalities and exercise therapy on myofascial pain syndrome by assessing the degree of effect size (ES) and related variables.

Dermabrasion involves the removal of the epidermis and the upper dermis by means of a motor-driven rotary abrasive instrument or a brush using ethyl chloride or dichlorotetrafluoroethane(Freon) as the evaporative refrigerant-anesthetic. Kurtin(1952) developed this refrigeration-abrasion method and named it skin planing. The technique of skin planing was introduced to Korea in early 1960s and it was extensively used for corrective surgery of scar induced by small pox until early 1970s. The indication for dermabrasion includes correction of scars, prophylaxis and correction of aging of the skin, removal of congenital nevoid anomalies, malignant and benign skin tumors, tattoos and others. The authors dermabased the cutaneous lesions of xeroderma pigmentosum, angiofibroma (adenoma sebaseum), nevus sebaceus of Jadassohn, epidermal verrucous nevus and linear porokeratosis, using Stryker' pneumatic powered dermabrader, with successful results. The technique of dermabrasion and the literature were briefly reviewed.
Aging ; Angiofibroma ; Cicatrix ; Dermabrasion* ; Dermis ; Epidermis ; Ethyl Chloride ; Korea ; Nevus, Sebaceous of Jadassohn ; Porokeratosis ; Skin ; Skin Diseases* ; Xeroderma Pigmentosum

PURPOSE: Allium sativum (AS) has been known to have widespread benefits in reducing some human cancer risk by immune stimualtion and anticarcinogenic activity. In the present study, we evaluated the preventive and antitumor properties of AS as an effective anticancer modifier for human prostate cancer in vivo. MATERIALS AND METHODS: Subcutaneous prostate cancers were established in athymic nude mice with 5x10(5) PC-3 human androgen-indenpendent prostate cancer cells. AS was injected at the site of tumor transplantation on day 1 and one week intervals up to 5 weeks (Experiment I), and into the established tumors sized by 50-60mm(3) weekly for 5 weeks (Experiment II). Therapeutic responses and efficacies of AS for prostate cancers in vivo were determined in separate controlled experiments, and definite histopathological studies were also performed. RESULTS: In vivo studies indicated statistically significant reduction in the incidence of tumor formation with programmed and continuous AS intralesional treatment. For established prostate cancer, AS treatment also demonstrated an inhibitory effect of tumor growth compared with control. Histomorphological and immunohistochemical studies demonstrated marked apoptosis after 5 weeks-AS continuous treatment in Experiment II. CONCLUSIONS: AS had a definite antitumor activity to inhibit tumorigenesis and may modulate tumor growth of prostate cancer in vivo. It is non-toxic, readily avaliable and inexpensive. AS, in the future, may be developed as a novel and effective treatment in chemoprevention for human prostate cancer.
Allium* ; Animals ; Apoptosis ; Carcinogenesis ; Chemoprevention ; Garlic ; Growth and Development* ; Humans ; Incidence ; Mice ; Mice, Nude ; Prostate* ; Prostatic Neoplasms*

The accessory ovary can be defined as an extraovarian tissue that is located near the normal ovaries and is connected to the broad ligament, infundibulopelvic ligament or utero-ovarian ligament. It has very rarely been reported. The majority was found during abdominopelvic surgeries for any other purposes, because they were usually small and less than 1 cm in diameter adn gave no particular symptoms related simply to their presence. We reported a case of accessory ovary in which developed a simple cyst of a largest diameter of 12 cm, and discussed the significance of the accessory ovary in clinical and pathological aspects.
Cysts

The objective of this study was to establish a good methodology to isolate single smooth muscle cells that are alive and respond properly to pharmacological agents. Canine urinary bladders were employed as the source of single cells, and acetylcholine, atropine and imipramine were used as indicators of pharmacological responsiveness. Imipramine, an antidepressant drug exhibited the anticholinergic and calcium antagonizing properties on rat detrusor muscle. To establish a control value for a further experiment to elucidate the mechanism of action of imipramine on detrusor muscle, we measured the concentration-response of single cells to acetylcholine in the presesnce of imipramine by length of the cells and compared the result with the response in the presence of atropine. Tiny chops of smooth muscle taken from anesthetized canine urinary bladder were incubated in collagenase solution at 36℃ for 17-20 minutes. The collagenase solution included collagenase 1.2 mg/ml, soybean tryspin inhibitory 0.08 mg/ml, bovine serum albumin 2% in 10 ml Krebs-Henseleit buffer solution aerated with a consistent breeze of 95/5% O2/CO2 to maintain the pH at 7.4. After washing with plain K-H solution on 450 mesh, cells were dissociated from the digested tissue for 12-15 minutes. Cell suspension was transfered in 5 ml test tubes and acetylcholine was added for the final concentration to be 10⁻¹⁴~10⁻⁹M. To find the optimal time to fix the cells to determine the contractile responses, 1% acrolein was added 5, 10, 20, 30, 60 and 120 seconds after the administration of ACh. The length of cells fixed by acrolein were measured by microscaler vis CCTV camers on phaes-contrast microscope. The average length of 50 cells from a slide glass was taken as the value of a sample at the very concentration point. Single cells were isolated from canine detrusor. The length of untreated cells varied from 82 µm to 94 µm. The maximal response to actylcholine 10E-9M was accomplished within 5 seconds of exposure, and the shortening was 19±3%. Atropine reduced the contraction of the cells concentration-dependently. Lmipramine which exerts a cholinergic blocking action on some smooth muscles also reduced the contraction concentration-dependently and by a similar pattern as atropine. These findings document that imipramine may exerts a cholinergic blocking activity in the single smooth muscle cells isolated from canine urinary bladder.
Acetylcholine ; Acrolein ; Animals ; Atropine ; Calcium ; Collagenases ; Glass ; Hydrogen-Ion Concentration ; Imipramine* ; Muscle, Smooth ; Myocytes, Smooth Muscle ; Rats ; Serum Albumin, Bovine ; Soybeans ; Urinary Bladder

The case of a 56-year-old woman with a keratoacarithoma(KA) of the labial mucosa is herein reported, The tumor was excised and the pathological features were characteristic of a KA, This case is reported because the oral mucosa is such an unexpected location for solitary KA and the labial mucosa of this patient received chronic actinic damage, which suggested an etiological relationship. Follow-up examination of the patient after four months showed no recurrence.
Actins ; Female ; Follow-Up Studies ; Humans ; Keratoacanthoma* ; Middle Aged ; Mouth Mucosa ; Mucous Membrane* ; Recurrence ; Solar System*

No abstract available.
Dermatitis, Contact*