Objective To study the expression of hypermethylated gene 1 protein and ovarian cancer gene 1 protein in ovarian cancer and its relationship with the pathological features of ovarian cancer,and to explore its significance in ovarian cancer.Methods Sixty-three cases of ovarian cancer specimens and 63 cases of normal ovarian tissue were taken from January 2014 to December 2015 at the First Hospital of Jilin University.Western blot was used to detect the expression of hypermethylated gene 1 protein and ovarian cancer gene 1 protein in ovarian cancer tissues and normal ovarian tissues,and to analyze the relationship between the expression of two proteins and ovarian cancer.Results The expression of hypermethylated gene 1 protein and ovarian cancer gene 1 protein in ovarian cancer tissues were significantly lower than that in normal ovarian tissues (P＜0.05).There was no significant difference in the expression of hypermethylated gene 1 protein in different staging,different degree of differentiation and different pathological types in ovarian cancer (P＞0.05).The expression of ovarian cancer gene 1 protein in ovarian cancer stage Ⅰ was higher than that in stage Ⅱ and Ⅲ-Ⅳ (P＜0.05).The expression of ovarian cancer gene 1 protein in ovarian cancer stage Ⅱ was higher than that in stage Ⅲ-Ⅳ (P＜0.05).The expression of ovarian cancer gene 1 protein in high differentiation of ovarian cancer was significantly higher than that in moderately differentiated and poorly differentiated (P＜0.05).There was no significant difference between ovarian cancer gene 1 protein expression and poorly differentiated ovarian cancer (P＞0.05).There was no sig nificant difference in the expression of ovarian cancer gene 1 protein in different pathological types of ovarian cancer (P＞0.05).Conclusion Hypermethylatel gene,protein participate in the occurrence of ovarian cancer,the ovarian cancer gene 1 protein is only related to ovarian cancer clinical stage and degree of differentiation.

Objective To investigate the histochemical characteristics of muscle fiber in patients with Becker type muscular dystrophy (BMD) and its clinical significance.Methods Muscular biopsies were performed in 3 patients with BMD. The specimens were stained by HE and nicotinamide-adenine dinucleotid-tetrazole reducase (NADH-TR) methods and observed under light microscope. Normal muscles from healthy people were saved as controls.Results The slice from BMD patients showed varied sizes of muscular fibers, remarkable hypertrophy fibers, splitting fibers, targetoid fibers, lobulated fibers, whorled fibers and ring fibers. Targetoid fibers were also found using NADH-TR staining.Conclusions The degree of fiber necrosis in BMD is mild. Degeneration and proliferation are the main pathological changes, which predict mild clinical symptoms, long course of disease and favourable prognosis.

Objective To observe the clinical efficacy of fire-needle acupuncture in treating myofascial pain syndrome (MPS). Method Seventy-two patients with MPS were randomized into a treatment group and a control group, 36 in each group. The treatment group was intervened by fire-needle acupuncture, while the control group was by ordinary acupuncture. The short-form McGill Pain Questionnaire (MPQ) was observed before and after the intervention, and the clinical efficacy was compared. Result The total effective rate and markedly-effective rate were respectively 94.4% and 77.8% in the treatment group versus 80.6% and 30.6%in the control group. There was a significant difference in comparing the markedly-effective rate between the two groups (P<0.01). The MPQ item scores [pain rating index (PRI), present pain intensity (PPI), and visual analogue scale (VAS)] were significantly changed after intervention in both groups (P<0.05). After intervention, the MPQ item scores of the treatment group were significantly different from that of the control group (P<0.05). Conclusion Fire-needle acupuncture is an effective method in treating MPS.

BACKGROUND:Acute pancreatitis is a common inflammatory disease mediated by pancreatic acinar cel s injury, and is mainly characterized by leukocyte infiltration. N-acetylcysteine can control leukocyte migration and regulate inflammation in some serious inflammatory diseases. OBJECTIVE:To investigate the protective effects of N-acetylcysteine in rat model of acute pancreatitis caused by sodium taurocholate. METHODS:Ninety Sprague-Dawley rats were randomly divided into three groups:normal control group, acute pancreatitis group and N-acetylcysteine group. Except normal control group, acute pancreatitis model was established in the other two groups by retrograde injection of sodium taurocholate into major duodenal papil a. Rats in the N-acetylcysteine group were treated with N-acetylcysteine intravenously through the tail vein. RESULTS AND CONCLUSION:After acute pancreatitis model was established, plasma amylase levels in the models were significantly higher than that in the normal control rats (P<0.05). Interleukin-1β,-6,-10, and tumor necrosis factorαexpression levels were also obviously higher than that in the normal control rats (P<0.05). Immunohistochemical staining demonstrated that N-acetylcysteine was mainly expressed in the islet cel s, and the pancreatic expression of N-acetylcysteine was down-regulated at both the mRNA and protein levels during the course of acute pancreatitis. N-acetylcysteine administration significantly reduced plasma amylase levels, myeloperoxidase activity, pro-inflammatory cytokine production, and pancreas and lung tissue damages. Furthermore, N-acetylcysteine administration did not cause significant inhibition of nuclear factor-κB activation in the pancreas. N-acetylcysteine is capable of improving damage of pancreas and lung, and exerting anti-inflammatory effects in rats with severe acute pancreatitis.

Objective To observe the clinical efficacy of suspended moxibustion plus Chinese medication in treating icteric hepatitis. Method Ninety-two patients with icteric hepatitis were randomized into a treatment group and a control group, 46 in each group. The treatment group was intervened by suspended moxibustion plus oral administration of Chinese medication for supplementing yang, tonifying spleen, expelling dampness, and activating blood circulation;the control group was intervened only by the same Chinese medication. The symptom and sign score, and liver function items including TBil and ALT were observed, and the clinical efficacy was compared. Result The symptom and sign scores were significantly changed in both groups after intervention (P<0.05). After intervention, there was a significant difference in comparing the symptom and sign score between the two groups (P<0.05). The TBil and ALT levels were significantly changed after intervention in both groups (P<0.05). The recovery rate and total effective rate were respectively 87.0%and 95.7%in the treatment group versus 76.1%and 82.6%in the control group, and the inter-group differences were statistically significant (P<0.05). Conclusion Suspended moxibustion plus Chinese medication is an effective method in treating icteric hepatitis.

Objective:To observe the clinical efficacy of skin needling plus heat-sensitive moxibustion in treating chronic facial paralysis. Methods:Sixty patients with chronic facial paralysis were divided into an observation group and an acupuncture group by using the random number table, 30 in each group. Patients in the observation group were given skin needling plus heat-sensitive moxibustion; while patients in the acupuncture group were given conventional acupuncture. Results:Three treatment courses later, the total effective rate was 93.3% in the observation group versus 86.7% in the control group, and the difference was statistically significant (P<0.05). Conclusion:Skin needling plus heat-sensitive moxibustion can produce a more significant efficacy than conventional acupuncture in treating chronic facial paralysis.

Objective To observe the clinical efficacy of fire acupuncture plus herbal medicine in treating acute gouty arthritis. Method Eighty-nine patients with acute gouty arthritis were randomized into a treatment group of 45 cases and a control group of 44 cases. The treatment group was intervened by fire acupuncture plus orally taking herbal medicine, while the control group was by oral administration of Diclofenac sodium and Allopurinol. The average pain score was observed before and after intervention, and the clinical efficacies were compared between the two groups.Result The total effective rate was 95.6% in the treatment group versus 81.8% in the control group, and the difference was statistically significant (P<0.05). The average pain score was significantly changed in both groups after intervention (P<0.01). After treatment, there was a significant difference in comparing the average pain score between the two groups (P<0.05).Conclusion Fire acupuncture plus herbal medicine is an effective approach in treating acute gouty arthritis.

Objective: To investigate the differences of biochemical markers between the patients with pulmonary hypertension related to left heart disease (PH-LHD) and LHD; to explore the sensitive bio markers which may predict PH in LHD patients. Methods: A total of 355 LHD patients admitted to our hospital from 2014-01 to 2015-05 were enrolled. According to 2009 ESC/ERS guidelines, PH was deifned by pulmonary artery systolic pressure (PASP)>50 mmHg and patients were divided into 2 groups: LHD group,n=224 and PH-LHD group,n=131. The basic information with blood levels of biomarkers was recorded and their accuracy for predicting PH was analyzed. Results: The pathogenesis of LHD included 184 (51.83%) patients of coronary heart disease, 90 (25.35%) of dilated cardiomyopathy and 81 (22.81%) of cardiac valve heart disease. Compared with LHD group, PH-LHD group had increased ratio of NYHA III and IV degree (89.31% vs 45.54%), decreased LVEF [42.0 (33.0, 59.0) % vs 60.0 (42.0, 65.0) %], all P<0.001; PH-LHD group presented elevated blood levels of BNP, bilirubin, red cell distribution width (RDW), uric acid and cystatin C, while reduced lipoprotein (HDL), allP<0.001. PASP was positively related to biomarkers as BNP, bilirubin, RDW, uric acid and cystatin C, while negatively related to HDL. With the combination of BNP, direct bilirubin and RDW, the predictive value for PH-LHD under ROC curve was 0.828 with the sensitivity at 0.813, speciifcity at 0.708. Conclusion: Blood levels of biochemical markers were statistically different between the patients of PH-LHD and LHD; the combination of BNP, direct bilirubin and RDW showed the higher accuracy for predicting PH occurrence in LHD patients.

AIM: To investigate the effect of venous marker chicken ovalbumin upstream promoter-transcription factor Ⅱ (COUP-TFⅡ) expression on vascular endothelial cell senescence and its molecular mechanism.METHODS: The mRNA expression of COUP-TFⅡ in the human coronary artery endothelial cells (HCAEC) and human umbilical vein endothelial cells (HUVEC) was detected by RT-qPCR.After transfection with a COUP-TFⅡ siRNA (siCOUP-TFⅡ) to inhibit COUP-TFⅡ expression in the HUVEC, the senescence and proliferation of endothelial cells were evaluated by β-galactosidase staining, Western blot, CCK-8 assay and cell counting after treatment with 10-5 mol/L angiotensin Ⅱ (AngⅡ).The protein levels of Akt and p-Akt were determined by Western blot.RESULTS: Compared with the HCAEC, COUP-TFⅡ was significantly highly expressed in the HUVEC.Knockdown of COUP-TFⅡ via siCOUP-TFⅡ significantly induced endothelial cell senescence and inhibited endothelial cell proliferation and p-Akt level after treatment with AngⅡ at 10-5 mol/L.Furthermore, an Akt activator SC79 at 4 mg/L partly reversed the effect of siCOUP-TFⅡ on AngⅡ-induced endothelial cell senescence and proliferation.CONCLUSION: Knockdown of COUP-TFⅡ promotes endothelial cell senescence and inhibits endothelial cell proliferation, which might be partly regulated by Akt signaling.

Objective:To describe a method based on analysis of the histogram of intensity values pro-duced from the magnetic resonance imaging (MRI)for quantifying the degree of fatty infiltration. Methods:The study included 25 patients with dystrophinopathy.All the subjects underwent muscle MRI test at thigh level.The histogram Mvalues of 250 muscles adjusted for subcutaneous fat,representing the degree of fatty infiltration,were compared with the expert visual reading using the modified Mercuri scale.Results:There was a significant positive correlation between the histogram Mvalues and the scores of visual reading (r =0.854,P <0.001).The distinct pattern of muscle involvement detected in the pa-tients with dystrophinopathy in our study of histogram M values was similar to that of visual reading and results in literature.The histogram M values had stronger correlations with the clinical data than the scores of visual reading as follows:the correlations with age (r =0.730,P <0.001 )and (r =0.753, P <0.001);with strength of knee extensor (r =-0.468,P =0.024)and (r =-0.460,P =0.027) respectively.Meanwhile,the histogram Mvalues analysis had better repeatability than visual reading with the interclass correlation coefficient was 0.998 (95% CI:0.997 -0.998,P <0.001)and 0.958 (95%CI:0.946 -0.967,P <0.001)respectively.Conclusion:Histogram Mvalues analysis of MRI with the advantages of repeatability and objectivity can be used to evaluate the degree of muscle fatty infiltration.