The protecting effects of HZD, DZ and Mg~(2+) on isolated cultured rat li ver cells injured by CCl_4 was compared in this article. The results showed that HZD (15?l/ml)or DZ(10~(-4)M)or Mg~(2+)(4.8?10~(-3)M)added to the liquid culture of rat liver cells, inhibited the overloading of calcium ions and the leakage of LDH and GOT in hepatocytes effectively. The contents of Ca~(2+) ion were positively related to the leakage of enzymes.The intensity of inhibition effects to the calcium overloading and the enzyme leakage in HZD were situated between those in DZ and Mg~(2+).

Objective To investigate the difference of efficacy of interlocking nails and dynamic compression plate in tibial shaft fractures.Methods The clinical data of 137 cases with tibial shaft fractures were reviewed retrospectively.They were divided into two groups according to the various treatments which included interlocking nails group(72 cases with 79 fractures) and dynamic compression plate group(65 cases with 70 fractures).The efficacy,healing fineness rate,function score after operation and complications were compared.Results After operation,the patients were followed up 21.4 months on average.The postoperative callus time,weight-bearing limb time,healing time and postoperative fixation time taken in interlocking nails group was shorter than that in dynamic compression plate group [(2.36 ± 0.46) months vs.(3.12 ± 0.57) months,(2.42 ± 0.51) months vs.(3.14 ± 0.63) months,(7.65 ± 1.29) months vs.(9.33 ±1.54) months,(12.64 ± 3.76) months vs.(16.38 ± 3.15) months],and there was significant difference (P＜ 0.05).The healing fineness rate was 97.5％(77/79) in interlocking nails group,and 92.9％(65/70) in dynamic compression plate group,and there was nosignificant difference (x2 =1.76,P ＞0.05).The incidence of comphcations in interlocking nails group was higher than that in dynamic compression plate group[25％(2H9) vs.12.9％(9H0)],and there was significant difference(P ＜ 0.05).Condmions Interlocking nails have better biomechanical features with high union rate,low infective rate and good recovery of joint function.The clinical result of interlocking nails for the tibial shaft fractures is also better than that of dynamic compression plate.

Objective To assess the diagnostic value of spiral CT colonography(SCTC)with orally administered Mannitol as negative contrast in colorectal tumor.Methods 27 volunteers and 68 patients suspected of various kinds of colorectal diseases were examined.About 1500 ml isosmotic Mannitol as oral negative contrast agent was administered and then 20mg of Raceanisodamine hydrochloride was injected intramuscularly,three phase enhanced spiral CT scanning was performed 5 to 10 minutes later.imaging data were post-processed with multiplanar reformation(MPR),maximum intensity projection(MIP).All normal colorectal were correctly subarea and were measured intestinal wall thickness and maximum outer diameters,the CT values of bowel wall in different phases were measured either,the bowel with tumor as control,and the results were statistically calculated.SCTC was classifield into three Grades according to the effect of intestinal preparation.CT data of 61 cases colorectal tumors identified by pathology were retrospective analysed.Results SCTC was successfully done in all objects,the SCTC grading included Grade 1 in 52 cases,Grade 2 in 39 cases and Grade 3 in 4 cases.The total rate of satisfaction towards examinantion was 95.8%.The lesions in 60/61 cases were demonstrated clearly by SCTC,among them,there were carcinoma in 30,lyphoma in 8,abscess in 8,polyps in 8,metastases in 4 and carcinoid in 3.One case with polyp(less than 5 mm)was missed.The sensitivity and specificity of SCTC in diagnosing colorectal lesions were 98.36% and 78.9% respectively.27 cases with colorectal carcinoma were correctly staged in Duke and the accuracy was 90%.Conclusion SCTC is a simple,rapid,noninvasive and effective method for evaluating colorectal tumor.

Objective To analyse the stress distribution of distal-extension removable partial dentures supported by mini implants. Methods The finite element analysis models of conventional removable partial dentures ( CRPD) and mini implants supported removable partial dentures (ISRPD) were established by modular denture model. The stress distribution of abutments and supporting tissues was compared by finite element analysis software Abaqus 6.5.Results Under vertical loading, the stress of abutment teeth, mucosa of edentulous region and alveolar bone of ISRPD model was lower than that of CRPD model. Under oblique loading, the stress of each part of CRPD model and alveolar bone of ISRPD model significantly increased, while that of abutment teeth and parodontium of ISPRD model decreased. Conclusion The distal-extension removable partial dentures supported by mini implants work well in protecting the abutments and supporting tissues.

Objective To analyse the stress distribution of precision attachment dentures used in distal-extension edentulous cases. Methods The finite element models of conventional removable partial dentures and precision attachment dentures were established by modular denture model, and the models of precision attachment dentures were established with 4┬4 as one abutment or 43┬34 as two abutments. The stress distribution of abutments and supporting tissues was compared by finite element analysis software Abaqus 6.5.Results Under vertical loading or oblique loading, the stress of abutments and supporting tissues from attachment dentures with two abutments was significantly lower than that from attachment dentures with one abutment. The stress of conventional removable partial dentures significantly increased under oblique loading. Conclusion It is reasonable for the design of attachment dentures with two abutments for mandibular distal-extension edentulous cases.

Objective To establish three dimensional finite element model libraries of telescopic dentures and removable partial dentures by means of model libraries, and compare the characteristics of stress distribution among three prostheses. Methods Normal model libraries were imported and edited, and models of bilateral free-ended cases were established. Based on the model libraries of fixed partial dentures and removable partial dentures, the finite element models of telescopic dentures and removable partial dentures with medial or distal occlusal rest were established using softwares such as DELL Precision Graphics Workstation, UG 5.0, HyperMesh 7.0 and Abaqus 6.5, then finite element stress analysis was performed. Results The stress distribution on abutment root, parodontium, alveolar mucosa and alveolar bone was uniform. The maximum value of stress on the abutment root (6.205 Mpa) was much less than those of two removable partial dentures (18.41 Mpa and 34.49 Mpa). Conclusion For bilateral free-ended cases, the telescopic denture design is uniform in stress distribution with less stress on abutment root, which is healthy to the abutment root and surrounding tissues.

Objective To redevelop the finite element model libraries and to analyse the stress distribution of telescopic crown dentures. Methods The finite element model libraries were redeveloped based on the model libraries of normal dentition, fixed partial denture and removable partial denture. With the help of softwares such as DELL Precision Graphics Workstation, UG 5.0, HyperMesh 7.0 and Abaqus 6.5, the telescopic denture models on the bilateral free-ended case were imported and edited, then the finite element stress analysis was performed. Results The models were easily established. The maximum value of stress and its distribution on abutment root, periodontal membrane, alveolar mucosa, alveolar bone and dental prostheses were observed and studied by the finite element stress analysis. Conclusion The way to establish models of telescopic crown dentures by model libraries is feasible and reliable.

Objective 　To search the relative factors of cerebral damage, rebleeding, delayed cerebral ischaemia(DCI) and hydrocephalus after subarachnoid haemorrhage (SAH) by CT scans and clinical findings in acute phase. Methods　To analyse the relationship between cerebral damage after SAH and clinical findings: CT scans resulte, age, sex, blood pressure, hyponatraemia, therapeutic methods. Results　Cerebral damage were related to the pattern of distritution of SAH on brain CT and hyponatraemia. The high attack rate of rebleeding and DCI is related to presence of blood in the surface of brain, collection of blood in the ventricle, saccula aneurysms or cerebral arteriovenous malformation (AVM) (P＜0.01). Conclusions　To forecast of cerebral damage after SAH by study of CT scans showing and clinical findings have clincal significance. According to these findings, we may take some therapeutics to prevent the cerebral damage after SAH.

AIM: To establish a Reversed-Phase high performance liquid chromatography method for determination of plasma ropivacaine in dog.METHODS:The sample was deproteinated by acetonitrile and determined with RP-HPLC and ultraviolet detection.the analytical column was Intertsil C18 column((4.6) mm?250 mm,5 ?m).The mobile phase consisted of methanol:H_2O(5050),the detection wavelength was 215 nm and the flow rate was(1.4)(ml?min~(-1)),the column temperature was 20 ℃,the sensitivity was 0.02AUFS respectively.The volume of sample for detection is 10 ?l.RESULTS:The linear range was(0.1)-25(?g?ml~(-1))(r=(0.9992)). The recovery of ropivacaine was(91.2)%-(93.6)%,RSD was(2.10)%-(3.40)%(n=6).The detection limit was(0.05)(?g?ml~(-1)).the intra-day and interday precisions of assay for ropivacaine was(1.35)%-(2.88)% and(1.80)%-(3.76)%.The quantative analysis of ropivacaine in plasma was not interfered by impurities.CONCLUSION: The assay method was shown to be suitable for determination the concentration of ropivacaine in plasma.The method is simple,accurate,sensitive and reproducible.

Objective To investigate the clinical effect of repairing soft tissue defects of foot and ankle by sural neurovascular adipofascial flap.Methods Application of sural neurovascular adipofascial flap to repaire soft tissue defects of foot and ankle in 19 patients,inclouding 3 csaes with the soft tissue defects of medial malleolus and 16 cases of dorsum pedis from May,2006 to May,2013.The size of soft tissue defects was 3 cm ×3 cm-7 cm ×9 cm.The skin of the donor site was sutured directly.The recipient area was reshaped granulation and underwent free skin graft after the adipofascial flap survived.Results After followed up of 3-18 months,the adipofascial flaps were all survived,the recipient area was almost flat with the surrounding tissue,the donor area only leaving linear scar,the appearance and function of donor area and adopt area were satisfactory.The sensation of lateral dorsal region of foot decreased to S3,and the sensation of recipient site recovered to S2.There were some changes in pigmentation with the skin graft region.Conclusion Sural neurovascular adipofascial flap can be effectively repaired soft tissue defects of foot and ankle,and it can avoid irregularity of donor area and enlargement of adopt area,the appearance and function were satisfactory.