Objective To explore effect of psychological intervention on negative automatic thoughts,self acceptance and social support for patients with type 2 diabetic cataract.Methods Patients with diagnosed type 2 diabetic cataract(n=105)were divided into the research group(56 cases)and the control group(49 cases).The centrol group received routine treatment.The research group was given psychological and behavioral intervention based on routine treatment.They underwent evaluation by negative automatic thoughts questionnaire (ATQ),self-acceptance questionnaire(SAQ)and social support rate scale(SSRS)before intervention and in the 2th and 6th weeks after intervention.The results were subsequendy analyzed. Results ATQ score of the research group in the 2th and 6th weeks were lower than that of the control group(P＜0.05).ATQ score,total SAQ score,score of SA and SD factors,total SSRS score and score of objective support,subjective support and utilization of support were all lower than those before intervention from the 2th week in the research group.While these happened in the control group only in the 6th week(P＜0.05).Total SAQ score and score of SA and SD factor were lower than those of the control group(P＜0.05).Total SSRS score and score of objective support,subjective support and utilization of support were lower than those of the control group(P＜0.05).Relative analysis showed total score of social support and its factors was negatively related with ATQ score,but was positively related with SAQ score and score of SA and SD factors(P＜0.05).Conclusions Psychological and behavioral intervention could reduce the negative automatic thoughts,allevinte self acceptance and increase social support degree for patients with type 2 diabetic cataract.

Objective To compare the histological effects of 1.44-?m and 1.06-?m pulse Nd:YAG laser radiation on stomach wall tissues in dogs. Methods Five beagle dogs were enrolled in this study to received 1.44-?m(experiment group) and 1.06-?m(self-control group) pulse Nd:YAG laser radiation at the posterior wall of the stomach.Totally 19 dosage of radiation was used including a 0-mJ radiation.The dosage of every single pulse was 200,500,or 800 mJ,and the amount of the pulses was 1,5,10,20,40 or 80.The experiment was repeated for 5 times.Afterwards,the injured tissues was obtained and observed under a light microscope and the photomicrograph was taken at the meantime.Results In experimental groups,the widest and photoevaporated zone and coagulation zone were(1863.8?225.0) ?m and(885.1?82.9) ?m,and the deepest photoevaporated zone and coagulation zone were(1787.4?93.7) ?m and(1327.8?86.3) ?m,respectively;which were significantly higher than those in the control [widest and photoevaporated zone and coagulation zone:(576.8?30.8) ?m and(1275.8?140.1) ?m,t=12.672,P=0.000 and t=-5.366,P=0.001;deepest photoevaporated zone and coagulation zone:(105.0?16.3) ?m and(994.8?130.0) ?m,t=39.539,P=0.000 and t=4.773,P=0.002;respectively].Conclusions 1.44-?m Nd:YAG laser is suitable for vaporization and coagulation in stomach surgery.It shows higher ability in vaporization but lower ability of coagulation than 1.06-?m pulse Nd:YAG laser radiation,which,however does not affect the outcomes of wound cleansing and hemostasis.

OBJECTIVE To analyze the antibiotics usage in perioperative patients of oral surgery in our hospital.METHODS Adopted retrospective review,365 oral surgery inpatients with operation were investigated on medication,course of treatment,wound healing and so on.RESULTS The utilization of antibacterials accounted for 100%.Nitramisole,aminoglycosides,Cephalosporin and penicillins were the main kinds.Single use accounted for 18.63%(66 cases),the bigeminy for 43.29%(158 cases),the trigeminy for 27.12%(99 cases),the utilization with four or more drugs were 18.63%(40 cases);the average of treatment course was 8 days.CONCLUSIONS To strengthen the management of antibacterials,more education of the principle medicine,usage are helful for reducing surgical site infection.

It has recently been demonstrated that anti-angiogenesis drugs targeting every possible aspect of tumor angiogenesis effectively inhibit the growth, invasion and metastasis of breast cancer with low sideeffects and drug resistance. These types of anti-angiogenesis drugs have become a hotspot in the field of breast cancer research.

Objective To summarize the experience of diagnosis and management of regional portal hypertension. Methods The clinical manifestations, diagnostic methods and therapeutic modalities of 16 cases of regional portal hypertension were analyzed retrospectively. Results Among 16 patients with ~regional portal hypertension, 12 cases resulted from pancreatic diseases, and 4 cases were complicated with non-pancreatic diseases. The main clinical findings were splenomegaly in 16(100%), abdominal pain ~in 10(63%) , gastrointestinal bleeding in 7(44%) and abdominal masses in 3(19%). All had normal liver function test. The main diagnostic methods were ultrasonography(US), computerized tomography (CT) and endoscopy. Splenic vein thrombosis could be detected by color Doppler ultrasonography (7/7). ~Enhanced CT could demonstrate enlarged and tortuous short gastric veins, gastroepiploic veins, and ~coll- ~ateral vessels around splenic hilum(16/16). Isolated gastric varices (4/5) were revealed mainly by ~gastro- ~scopy . Splenectomy was effective for controlling gastrointestinal bleeding, and its complication of portal vein thrombosis occurred in 1 case. Conclusions It is not difficult to diagnose regional portal ~hyper- ~tension based on findings of US and CT, together with special clinical characteristics. Therapeutic options should be ~individualized according to underlying diseases, however, splenectomy should be performed in the ~patients with gastrointestinal bleeding.

Object To provide a basis for the identification and development of Echinacea angustifolia DC Methods Pharmacognostical studies were carried out by phytotomy, macroscopic and microscopic analysis Results Detailed description of its pharmacognostical characteristics were accomplished and described Conclusion The results can be used for quality standardization of commercial product of E angustifolia

Background and purpose:Hereditary nonpolyposis colorectal cancer(HNPCC) which related to inheritance is an autosomal dominant inheritance.There are many screening standards differing presently from country to country.The Amsterdam standard is internationally recognized.These screening standards are just based on family history and clinical manifestations which can not reflect the essence of the disease.At present,the golden standard for screening HNPCC is mismatch repair genes detection that is in the experimental stage.The purpose of this study was to investigate the expression of mismatch repair genes hMSH2 and hMLH1 in HNPCC and their clinicopathological signif icance,and to fi nd a simple,fast and economic method for screening HNPCC.Methods:Immunohistochemical staining was used to detect the expression of hMSH2 and hMLH1 in 20 cases with HNPCC(Group A),20 cases with ordinary hereditary colorectal cancer(Group B),20 cases with sporadic colorectal cancer(Group C),and 20 cases with colorectal polyps(Group D).Results:The expression rates of hMSH2 and hMLH1 in Group A,B,C and D were 35%(7/20),70%(14/20),95%(19/20) and 100%(20/20) respectively,which illustrated that the probability of HNPCC and the negative expression of hMSH2 and hMLH1 was reduced from Group A to Group D.There were signifi cant differences among these groups(P0.05).Conclusion:The negative expression of hMSH2 and hMLH1 was signifi cantly correlated with HNPCC and location and differentiation of colon carcinoma.Immunohistochemical staining may be useful for screening HNPCC.

Objective To establish the high myopic choroidal neovascularization (CNV) in guinea pigs and to investigate the role of (matrix metalloproteinase-2,MMP-2) and tissue inhibitor-2 of metalloproteinase (TIMP-2) in high myopic CNV.Methods Seventy-two 2-week-old guinea pigs were randomized into control group (n =36) and high myopia group (n =36).Right eyes were indued form deprivation high myopia for 6 weeks.Thirty guinea pigs were randomly selected in each group,and CNV were induced in the right eyes br the 532 nm laser.MMP-2 and TIMP-2 expressions were investigated by immunohistochemistry pre-laser and 7,14,21,28,35 days after laser induction,respectively,while MMP-2 and TIMP-2 relative expression levels in retinal pigment epithelium (RPE)-choroid-sclera complex were detected by real-time PCR.Integral opitical density (IODs) of positive expression and mRNA relative expression levels of these factors were performed by statistical analyses.Results The expressions of MMP-2 and TIMP-2 were up-regulated in the two groups after laser photocoagulation through immunohistochemistry and real-time PCR examinations.Expression of MMP-2 peaked at 21 d and TIMP-2 at 28 d,respectively.IODs of positive expression and mRNA relative expression levels of MMP-2 were higher in high myopia group than those in control group at each inspective time point,and the differences were statistically significant (P ＜ 0.05).TIMP-2 expression was significantly reduced in high myopia group compared with control group before laser photocoagulation (P＜0,05),while there was no significant difference between the two groups at each time point after laser photocoagulation.Conclusions MMP-2 and TIMP-2 were closely related to the formation of high-myopic CNV.Balance disorders of MMP-2 and TIMP-2 might participate in the occurrence and development of high-myopic CNV.

Objective To explore the TCM pathogenesis regularity of nonalcoholic fatty liver disease (NAFLD);To provide basis for clinical syndrome differentiation and treatment. Methods A cross-sectional study was used. The clinical observation form of NAFLD was used to collect the patient data. Factor analysis and singular value decomposition methods were used to study the internal factors of the four diagnostic characteristics. The differences between the various analytical methods were compared. Results Factor analysis used the α factor analysis method, the principal component method, and the image factor method. The results of α-factor analysis were the best. The first three factors had the highest explanatory rates of data, namely spleen-dampness factor, kidney deficiency factor and qi deficiency factor, which constituted the basic pathogenesis factor of NAFLD. The singular value decomposition method obtained 3 singular values, namely spleen and kidney qi deficiency, liver and kidney yin deficiency, and qi and yin deficiency, also highlighted the spleen and kidney deficiency in the pathogenesis of NAFLD. Conclusion Spleen-kidney deficiency plays an important role in the pathogenesis of NAFLD. Treatment based on deficiency for nonalcoholic fatty liver disease has great clinical significance.

OBJECTIVE： To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS： External standard method was used The eluation of fentanyl was carried out by shim－ Pack CLC－ ODS（ 6 0mm× 150mm， 5μ m） column with 0 015mol/L NaH2PO4 acetonitril－ H2O（ 30∶ 70， v/v） as the mobile phase， and detection wavelength was 195nm RESULTS： Calibration curve for fentanyl was linear in the range of 2 0～ 100ng/ml（ r=0 999）  The detection limit was 1 0ng/ml The within－ day RSD was （ 6 50± 2 79） ％ ， the between－ day RSD Was （ 6 70± 3 04） ％  The method recovery was （ 91 70± 4 70） ％  The extraction recovery was （ 97 38± 3 69） ％  CONCLUSION： This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl