BACKGROUND: Primary hypertension is in the category of "dizziness"and "dementia" in Chinese medicine. It is discovered in recent years that vascular dangerous factors, like hypertension affect the risk constitution of Alzheimer disease.OBJECTIVE: To probe into relationship between cognition and syndrome differentiation of primary hypertension.DESIGN: Case-control analysis was designed.SETTING: Jiangsu Staff University of Medical Science and Yangzhou Wenhe Hospital.PARTICIPANTS: Totally 90 cases of primary hypertension were employed from May to June 2000. They were from some city in the north of Jiangsu province, graduated from middle school and cooperated with the investigation. Totally 30 cases were collected from physical-check group and taken in the control.METHODS: The cases of primary hypertension were differentiated as excess-syndrome group (excessive phlegm and damp and hyperactivity of liver yang), deficiency-syndrome group (yin deficiency of liver and kidney and deficiency of yin and yang), excess within deficiency group (complicated with blood stasis and with turbid phlegm) and the control group. Neuropsychological method was used to evaluate the cognition.MAIN OUTCOME MEASURES: Indexes of neuropsychology: Fuld object-memory Evaluation (FOM), rapid verbal retrieve (RVR), block design WISE (BD), digit span WISE (DS), Hachinski ischemic score (HACH), mini-mental state examination (MMSE) and activity of daily living scale among groups.RESULTS: The data of 90 cases of primary hypertension and 30 cases in physical-check group entered result analysis. Compared with the control group, significant difference was not indicated in every statistical index in excess-syndrome group (P ＞ 0.05). In deficiency-syndrome group, except MMSE and ADL, the rest observed indexes indicated statistical difference.In excess within deficiency group, statistical difference presented in every observed index (P ＜ 0.01), in which the results in the complication of turbid phlegm was worse than blood stasis (FOM was 11.3±3.7,13.5±2.5 respectively; DS was 6.4±3.9,8.3±3.5, respectively)CONCLUSION: Primary hypertension affects mental state of patient, in which, cognition is declined mildly in deficiency-syndrome group. Dementia symptoms present in excess within deficiency group which is severe in phlegm type compared with blood stasis type. Differentiation of syndromes in Chinese medicine integrated with neuropsychological assessment benefits early discovery and diagnosis of dementia.

Objective:To investigate the inhibitory effects of human angiostatin ( AS) on the subcutaneously transplanted mouse glioma. Methods; Effects of AS on the proliferation of angioendothelial cell line ECV-304 and human glioma cell line TJ-905 were observed with MTT method. Mouse model of subcutaneously transplanted of glioma line G422 was established. AS was injected subcutaneously, tumor ratio and tumor in hibitory rate were estimated. Results; ( 1) Inhibitory effects of AS on ECV-304 enhanced with the increase of dose, while AS exerted no effect on TJ-905 ; (2) It was shown with animal experiments that turner inhibitory rates were 21. 8% and 84. 3% with AS doses of 10 mg and 50 mg-kg-1. d-1 , respectively; (3) Immunochemical factor VM staining indicated there the differences between the control and the control and the test groups in both the angiodevelopment and quantity. Conclusion; There was no inhibitory effect for AS to angiolorna cell in vitro, however in vivo, it inhibited the glioma growth via its inhibitory effect on the proliferation of an-gioend othelial cell line. It was then suggested that AS would be of wide application in tumor treatment.

BACKGROUND:Stromal cel-derived factor-1 (SDF-1) and chemokine receptor-4 (CXCR4) play an important role in stem cel homing during bone marrow, organ and tissue transplantation. But there are few reports on limbal stem cel transplantation and homing as wel as the distribution and content of SDF-1/CXCR4 on the cornea. OBJECTIVE:To detect the expression levels of SDF-1/CXCR4 mRNA in rat corneal tissue by fluorescence quantitative PCR. METHODS: The normal rat cornea was taken completely, and subdivided into central cornea, peripheral corneal and limbal area. Fluorescence quantitative PCR method was employed to detect SDF-1/CXCR4 mRNA levels at corresponding positions. RESULTS AND CONCLUSION: The expression levels of SDF-1 and CXCR4 mRNA were 0.213±0.015 and 0.189±0.02 in the central cornea, 0.529±0.077 and 0.285±0.015 in the peripheral cornea, 0.666±0.069 and 0.258±0.067 in the limbal area, respectively. These data show that there are significant differences in the expression levels of SDF-1 and CXCR4 mRNA in the central cornea, peripheral corneal and limbal area, and these differences exert an important role in cel homing and migration during limbal stem cel transplantation for treatment of limbal stem cel deficiency.

BACKGROUND:How to promote homing of scarce limbal stem cel s is the key to improving transplantation efficiency. And stromal cel-derived factor-1 plays an important role in the repair of retinal pigment epithelial cel s injury. OBJECTIVE:To observe changes of stromal cel-derived factor-1 levels in rat and mouse limbal stem cel deficiency models in order to confirm whether these changes wil induce peripheral blood stem cel s homing into the cornea. METHODS:Eye models of limbal stem cel deficiency were prepared in rats and mice by cutting method, then peripheral blood samples were continuously drawn to detect the mass concentration of stromal cel-derived factor-1 by enzyme-linked immunosorbent assay within 1 month after modeling. RESULTS AND CONCLUSION:In the rat peripheral blood, the mass concentration of stromal cel-derived factor-1 started to increase at 24 hours after modeling and firstly peaked at the 3rd day;then it tended to decrease and reached a second peak lower than the first one at the 25th day;subsequently, it returned to the normal level. In the meanwhile, the mass concentration of stromal cel-derived factor-1 in the mouse peripheral blood, increased at 12 hours after modeling, peaked at the 3rd day, subsequently decreased until another peak lower than the former one presented at the 20th day, and final y it returned to the normal level. In conclusion, the level of stromal cel-derived factor-1 in the peripheral blood is significantly associated with limbal stem cel s deficiency, which may play an important role in corneal injury repair.

Objective To investigate the effect of heme oxygenase-1 ( HO-1) on brain edema in a rat model of asphyxial cardiac arrest and resuscitation. Methods Forty male SD rats weighing 250-300 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation; group II cardiac arrest (CA); group Ⅰ Hemin (HO-1 inducer) and group IV SnPP (HO-1 inhibitor). Asphyxial cardiac arrest and resuscitation were performed in CA, Hemin and SnPP groups (group Ⅰ ,Ⅲ, Ⅳ) . Hemin and SnPP groups received hemin 15 mg/kg intraperitoneally (IP) at 12 h before CA and SnPP K 30 μmol/kg IP at 1 h before CA respectively. The animals were sacrificed at 1 and 6 h after recovery of spontaneous circulation (ROSC) . The water content of the cortex, hippocampus and brain stem and the expression of HO-1 and aquaporin-4 (AQP4) mRNA in cortex and hippocampus ( by RT-PCR) were determined. Results Water content of cortex and hippocampus was significantly higher at 1 h after ROSC in CA and SnPP groups than in sham operation group and was significantly lower in Hemin group than in CA group. There was no significant difference in water content of brain stem at 1 and 6 h after ROSC among all 4 groups. The expression of AQP4 mRNA was significantly higher in cortex and hippocampus at 1 h after ROSC in CA and SnPP groups than in sham operation group and was significantly lower in Hemin group than in CA group.Conclusion HO-1 can reduce brain water content at early stage after cardiac arrest and resuscitation by regulating the expression of AQP4.

Objective To study clinical effect of transplantation with vascularized ilium bone and free fibulae, and internal fixation of titanium plate on reconstruction of maxillary defect. Methods Nine cases of maxillary defect were repaired by 7 iliac muscle flaps with deep circumflex iliac artery and vein, 2 cases of fibulae muscle flap with peroneal artery and vein, meanwhile, fixed internally by titanium plate. The vessels were anastomosed with arteria and vena maxillaries externa. Results Nine patients were confirmed by 99m TcMDP 2-4 weeks after operation that the supply of blood was well and the graft bones were survival. By follow-up from 6 months to 3 years after operation, the fixations of graft bones were good. Conclusion Vascularized bone graft and internal fixation of titanium plate is one of the best ways for reconstruction of maxillary defect.

Objective To evaluate effects of pulmonary surfactant (PS) containing ketamine on respiratory failure induced with lung-lavage in rats Methods Twenty Wistar rats were anesthetized with intraperitoneal pentobarbital sodium and were ventilated with the peak inspiratory pressure(PIP) being 1 47 kPa and E:I at 1:1 following intubation through tracheotomy In all rats whole double lung lavage were performed with normal saline (37℃, 40 ml?kg -1 ) 8 10 times after PIP and PEEP were adjusted to 1 96 kPa and 0 49 kPa respectively The rats were randomly divided into A and B groups after PaO 2 decreased to less than 12 kPa In group A (n=10): PS (25mg, 0 5ml) was injected intratracheally to lungs; in group B (n=10): PS (25mg, 0 5ml) containing ketamine 2 5 mg was injected At 120th min after PS injection, PIP and PEEP were gradually adjusted to 1 47 kPa and 0 kpa (ZPEEP), respectively Results PaO 2 in both groups decreased significantly from 59 3 kPa to 10 3 kPa after lung lavage(P0 05 vs pre-lavage) All rats in group B and 4 of 10 rats in group A survived at the end of experiment Conclusions Intratracheal administration with PS containing ketamine not only reverses hypoxemia , but also maintains the respiratory function under low airway pressure without PEEP after respiratory failure induced with lung lavage,

0.05). After the injection, PaO 2 in group A, B and C increased significantly, whereas PaO 2 in group D did not change and was kept at low level below 13.3kPa (P

Objective To study the correlation between postoperative tumor recurrence and VEGF expression in preoperative biopsy specimens of early gastric carcinoma together with p53 gene abnormality and tumor cell proliferation. Methods Sixty-eight endoscopically biopsied specimens obtained preoperativelly from patients with early gastric carcinoma were studied immunohistochemically. Date was processed by computer image analysis system. Result The rate of VEGF positivity was significantly higher in patients (n=5) with postoperative tumor recurrence than in those (n=63) without (5/5 vs. 14/63, P

Bone marrow mesenchymal stem cells(BMSCs) are a population of multipotent cells. Many researchers have studied whether BMSCs could differentiate into neurons in vivo and ex vivo. Although some studies didn't agree with about some chemical induction medium inducing neurons, the application of cell transplantion and gene therapy of BMSCs has achieved certain progress showing good future for BMSCs in therapy of nervous system diseases.