Objective To explore the expression of lectin-like oxidized low-density lipoprotein receptor-1 ( LOX-1) in a rat model after balloon arterial injury and investigate the effect of cilostazol on the mRNA and protein expressions of LOX-1. Methods Twenty-four male SPF SD rats were randomly divided into 3 groups ( n =8 for each group) ,sham operation group,balloon arterial injury group and cilostazol treatment group. For the rats from balloon arterial injury group and cilostazol treatment group,the left common carotid artery were in- juried by a balloon catheter. The expression of LOX-1 mRNA and protein in neointimal hyperplasia after balloon arterial injury were examined by RT-PCR and immunohistochemistry respectively. The contents of MDA in blood serum was examined by TBA. Results LOX-1 mRNA and protein were expressed in the neointimal hyperplasia after balloon arterial injury but not in the sham-operation group. The expression level of LOX-1 was significantly lower in the cilostazol treatment group than that in the model group ( P

Objective To evaluate the effect of moderate activities at moderate high altitude on acute mountain sickness (AMS) incidence .Methods Ninety-one healthy sea level residents traveled from sea level (345 m) to high altitude city (3200 m) ,by train within 48-hour .They walked 5 kilometers after 2-night stayed ,Lake Louis Score (LLS) Questionnaires ,blood pressure(BP) and oxygen saturation (SpO2 ) was administered before and after walking .Results Seven subjects were excluded because of incomplete data .The incidence of AMS before and after exercise was 20 .24% (n=17/84) and 11 .90% (n=10/84) respectively(P>0 .05) .Af-ter a 5 kilometer walking ,the heart rate increased from (73 .83 ± 9 .96)bpm to(84 .31 ± 12 .55)bpm (P<0 .05) ,Systolic BP and SpO2 level decreased from(128 .86 ± 13 .93)mm Hg to (124 .48 ± 13 .13)mm Hg ,(92 .80 ± 2 .25)% to (89 .94 ± 2 .45)% (P<0 .05) .Headache symptom improved after walking (P<0 .05) .Smoking was negative correlate with LLS score before and after ex-ercise(P<0 .05) .There is no relation between SpO2 and LLS scores .Conclusion Walking five kilometers at 3200 m improve head-ache symptom and tend to decrease AMS .

AIM: To establish an animal model of experimental autoimmune myocarditis (EAM) in BALB/c mice and to investigate the expression and significance of Toll-like receptor 3 in mouse EAM. METHODS: BALB/c mice were immunized with cardiac myosin extracted from porcine ventricular myocardium covered by complete freund's adjuvant (CFA) on 0 d and 7 d, then divided into immunized with CFA only. Serum and myocardium samples were collected at 14 d and 21 d after the first immunization. HE staining was used to identify the areas of inflammation. The myosin IgG antibody was examined by indirect ELISA assay. The changes of TLR3 protein and mRNA expression in myocardial tissue were measured by immunohistochemistry and real time-PCR. RESULTS: Compared to control group, immunohistochemistry results showed that there was positive expression of TLR3 in the myocardium of mice with EAM and the mRNA of TLR3 were more than 20 times (P<0.05). The expression of interferon beta mRNA in EAM group was more than 14 times as many as basal expression, that of tumor necrosis factor alpha was more than 18 times (P<0.05). CONCLUSION: The expression of Toll-like receptor 3 in myocardium is up-regulated in experimental autoimmune myocarditis. The inflammatory response to cardiac myosin may associate with the TLR3 signal transduction pathway.

Objective To investigate the dynamic changes of signal transducer and activator of transcription 6(STAT6)and peroxisome proliferator-activated receptor alpha(PPARα)in peripheral blood of patients with acute cerebral in-farction(ACI)and their association with disease severity and prognosis.Methods A total of 86 ACI patients who were hospi-talized in our hospital from January 2021 to June 2022 were enrolled as ACI group,and according to the National Institutes of Health Stroke Scale(NIHSS)score,they were divided into mild group with 33 patients,moderate group with 35 patients,and severe group with 18 patients;a total of 85 individuals who underwent physical examination were enrolled as control group.ELISA was used to measure the level of STAT6 in peripheral blood;quantitative real-time PCR was used to measure the mRNA level of PPARα in peripheral blood;an automatic biochemical analyzer was used to measure blood-related biochemical param-eters,and an automatic blood pressure instrument was used to measure blood pressure parameters;a Pearson correlation analy-sis was used to investigate the correlation of STAT6 and PPARα levels with NIHSS score on admission.Results There were significant differences between the control group and the ACI group in triglycerides,low-density lipoprotein cholesterol,lipo-protein,diastolic pressure,and systolic pressure(P<0.05).The ACI group showed gradual reductions in the levels of STAT6 and PPARα in peripheral blood on days 1,3,and 7 of stroke,with the lowest levels observed on day 7 of stroke,and there were increases in the levels of STAT6 and PPARα on day 14 of stroke,which was still lower than the levels in the control group(P<0.05).For the ACI patients,the expression levels of STAT6 and PPARα in peripheral blood tended to decrease gradually with the aggravation of disease conditions at different time points of stroke.The Pearson correlation analysis showed that the levels of STAT6 and PPARα in peripheral blood of ACI patients on day 1 of stroke were negatively correlated with NIHSS scores(P<0.05).The good prognosis group had significantly higher levels of STAT6 and PPARα than the poor progno-sis group at different time points of stroke(P<0.05).Conclusion There are reductions in the levels of STAT6 and PPARα in peripheral blood of ACI patients after the onset of ACI,which tend to first decrease and then increase over the time of stroke,and the changes in the levels of STAT6 and PPARα are associated with the severity and prognosis of ACI patients.