Objective:To investigate the effect of pulmonary rehabilitation therapy on the exercise capacity and dyspnea of persons with chronic obstructive pulmonary disease (COPD).Methods:One hundred COPD patients were randomly divided into a control group and an observation group, each of 50. Both groups were given routine medication, while the observation group was additionally provided with health guidance, oxygen therapy, respiratory physiological therapy and exercise for 3 months. Before and after the intervention, both groups′ forced expiratory volume in 1 second (FEV1) and the first and second forced expiratory volume as a percentage of FEV (FEV1%) were measured. The subjects′ motor functioning was evaluated using the 6-minute walk test. Enzyme-linked immunosorbent assays and immunoturbidimetry quantified their expression of inflammatory factors. And their ability in the activities of daily living (ADL) was evaluated using the Barthel index. The COPD quality of life questionnaire (CRQ) was also used to assess their life quality.Results:After the intervention, the average clinical efficacy in the observation group was 96%, significantly higher than that of the control group (80%). Moreover, the average FEV1, FEV1%, 6-minute walk test time of the former group were all significantly better than before the intervention and better than the control group′s results after the intervention. Their average CRP, IL-6 and TNF-α levels were all significantly lower as well. After the intervention, the observation group′s average total CRQ score and its average scores on the instrument′s emotion, fatigue, wheezing and disease control components were all better than the control group′s averages. The observation group′s average ADL score was also significantly higher than that of the control group.Conclusions:Supplementing conventional medication with pulmonary rehabilitation therapy can effectively improve the lung function, motor functioning and life quality of COPD patients. It can also lower their level of serum inflammatory factors.

AIM: To establish an animal model of experimental autoimmune myocarditis (EAM) in BALB/c mice and to investigate the expression and significance of Toll-like receptor 3 in mouse EAM. METHODS: BALB/c mice were immunized with cardiac myosin extracted from porcine ventricular myocardium covered by complete freund's adjuvant (CFA) on 0 d and 7 d, then divided into immunized with CFA only. Serum and myocardium samples were collected at 14 d and 21 d after the first immunization. HE staining was used to identify the areas of inflammation. The myosin IgG antibody was examined by indirect ELISA assay. The changes of TLR3 protein and mRNA expression in myocardial tissue were measured by immunohistochemistry and real time-PCR. RESULTS: Compared to control group, immunohistochemistry results showed that there was positive expression of TLR3 in the myocardium of mice with EAM and the mRNA of TLR3 were more than 20 times (P<0.05). The expression of interferon beta mRNA in EAM group was more than 14 times as many as basal expression, that of tumor necrosis factor alpha was more than 18 times (P<0.05). CONCLUSION: The expression of Toll-like receptor 3 in myocardium is up-regulated in experimental autoimmune myocarditis. The inflammatory response to cardiac myosin may associate with the TLR3 signal transduction pathway.

Objective:To observe efficacy and mechanism of salvia miltiorrhiza and tetramethylpyrazine in the treatment of acute exacerbation of chronic obstructive pulmonary disease(COPD) accompanied by pulmonary heart disease .Methods:A total of 90 patients with acute exacerbation of COPD accompanied by pulmonary heart disease were randomly selected and divided into two groups ,the salvia miltiorrhiza and tetramethylpyrazine group and the control group ,with 45 cases in each .Both groups were given symptomatic treatment ,and the salvia miltiorrhiza and tetramethylpyrazine group received 5 mL salvia miltiorrhiza and tetramethylpyrazine injection(dissolved in 250 mL 5% glucose solution) by intravenous infusion once a day in addition .Both the two groups have been treated for 15 days .The blood gas analysis indexes(PaO2 ,SaO2 ) ,the hemodynamic indexes(PASP ,PADP ,MPAP) ,and the vasoactive factor indexes(ET‐1 ,NO) of the patents in two groups were detected before and after the treatment .Results:There was no statistically significant difference between the two groups in each index before the treatment .Each index of patients in both two groups improved after the treatment ,compared with that before the treatment(P< 0 .05、0 .01) , of which the patients in salvia miltiorrhiza and tetramethylpyrazine group improved more significantly .Conclusions:Salvia miltiorrhiza and tetramethylpyrazine showed good efficacy on acute exacerbation of COPD accompanied by pulmonary heart disease .It could improve the oxygen supply for lung tissue ,reduce the generation of ET‐1 , increase the release of NO ,reduce the PASP and improve the cardiopulmonary function .

Objective:To investigate the current situation of basic knowledge and diagnosis and treatment behavior for chronic obstructive pulmonary disease (COPD) among physicians of South Henan area .Methods:Questionnaire was designed for a research ,which was conducted among physicians from 15 hospitals located in 9 counties ,and the data were analyzed by using SAS 6 .12 software .Results:A total of 135 valid questionnaires were collected .And 80 .7% (109/135)of the physicians understood the epidemiology of COPD ,while 31 .8% (43/135) of them understood the disease characteristics .The proportion of physicians who understood the disease characteristics in respiratory professional group was 44 .4% (20/45) ,which was significantly higher than that (25 .5% ,23/90) in non‐respiratory professional group (P<0 .05) .Furthermore ,66 .6% (90/135) of the physicians diagnosed disease by clinical symptoms ,while 33 .3% (45/135) of them diagnosed disease by clinical symptoms and pulmonary function .The proportion of physicians who diagnoses disease by clinical symptoms and pulmonary function in respiratory professional group was 60 .0% (27/45) ,which is significantly higher than that 20 .0% (18/90) in non‐respiratory professional group (P< 0 .05) .Therapy for acute exacerbation of COPD was nearly conformed to the treatment guidelines ,however ,50 .3% (68/135)of physicians did not completely understand mechanical ventilation .For therapy of stable COPD ,57 .7% (78/135) of physicians recommended inhaled glucocorticosteroids combined with beta‐2‐receptor agonist .The proportion of physicians who recommended inhaled glucocorticosteroids combined with beta‐2‐receptor agonist in respiratory professional group was 88 .8% (40/45) ,which was significantly higher than that 42 .2% (38/90) in non‐respiratory professional group (P<0 .05) .Non‐respiratory professional group were lack of knowledge of home oxygen therapy ,respiratory exercises , and nutritional support .Only 11 .1% (10/90) physicians in the non‐respiratory professional group understood the knowledge comprehensively .Conclusions:It is key to enhance the education of COPD and popularize the treatment guideline among the physicians ,especially non‐respiratory physicians ,in South Henan area .