Objective To evaluate the value of multislice CT scan-3D reconstruction in the clinical type and treatment of ankle fracture.Methods Ankle fractures in 89 cases,preoperative underwent plain radiography,spiral CT and three-dimensional reconstruction at the same time.According to the Lange-Hansen classification and DanisWeber classification of ankle fractures were classified,the evaluation of 3D reconstruction with spiral CT in ankle fracture treatment in ratsResults The type of Lange-Hansen were as follows:pronation-abduction (PAB) 34 cases;pronation-external rotation(PER) 29 cases ; supination-adduction(SAB) 17 cases ; supination external rotation(SER) 9 cases.The type of Danis-Weber:A type 11 cases,B type 40cases,C type 38 cases.Conclusion Multislice CT scan-3D reconstruction has an important value in the clinical type and treatment of ankle fracture.

Objective:To compare the different characteristics of lipid profiles among the populations with normal glucose tolerance(NGT),impaired glucose regulation(IGR) and diabetes mellitus(DM).Methods:A cross-section study of glucose and lipid metabolism was conducted in a Chongqing population aged 21 to 89 years.According to the results of oral glucose tolerance test(OGTT),the subjects were divided into 3 groups: NGT group,IGR group and DM group.The metabolic features of the lipid in each group were analyzed by sex.Results:TG,TC and LDL-c levels gradually increased,but HDL-c levels decreased in the groups of NGT,IGR and DM.In NGT group,the prevalence of men with high TG was significantly higher than that of women.In IGR group,the prevalence of women with high TG was significantly higher than that of men.In DM group,there was no difference in the prevalence of high TG between two sexes.The prevalence of women with high TC was significantly higher than that of men in each group.Conclusion:The pattern of dyslipidemia among Chongqing populations is characterized by high TG,TC and LDL-c levels.Women,compared with men,show greater prevalence of high TC.

OBJECTIVE:To introduce the pharmacoeconomic evaluation into the selection of national essential drugs so as to improve the accessibility of drugs,use essential drugs rationally,and control the rapid increase of drug expenses.METHODS:The status quo of the application of pharmacoeconomic evaluation in the selection process of essential drugs in both China and abroad and the main problems faced by China were analyzed.RESULTS & CONCLUSION:The lack of evaluation agency and professional talents,non-unified technical standard and imperfect system guarantee are the main problems faced by China in the introduction of pharmacoeconomic evaluation into the selection of national essential drugs were,thus 5 thoughts on the introduction of pharmacoeconomic evaluation into the selection of National Essential Drugs have been put forward.

AIM: To explore whether human telomerase reverse transcriptase (hTERT) antisense oligodeoxynucleotide (ASODN) could enhance the sensitivity of acute lymphoblastic leukemia cells from relapse patients to cisplatin. METHODS: The expression levels of hTERT protein were detected by immunofluorescence using fluorescence isothiocyanate (FITC), the number of viable cells was determined using the trypan blue dye exclusion assay, and apoptosis was detected by morphological observation and flow cytometric cell cycle analysis. RESULTS: The expression of hTERT protein was inhibited after treatment with hTERT ASODN. Treatment with cisplatin combined with hTERT ASODN had significantly reduced the number of viable acute lymphoblastic leukemic (ALL) cells (P<0.05). In morphological observation of apoptotic cells using Hoechst33258 and PI double staining techniques, cells displayed classic apoptotic changes in the presence of cisplatin or cisplatin combined with hTERT ASODN or ASODN at 48 h. Apoptotic rates of cells treated with cisplatin and ASODN were higher than that of cells treated with cisplatin alone (P<0.05). CONCLUSION: hTERT ASODN could increase sensitivity of cultured primary acute lymphoblastic leukemia cells from relapse patients to cisplatin.

Musk ketone(3-methyl cyclopentadecanolide) is the main active ingredient of the artificial musk,it has multiple pharmacological effects,including anti-dementia,cerebral ischemia,anti-inflammatory effects and so on.This article outlines the main pharmacological effects of musk ketone on brain diseases in the recent years, aim to further research, investigation and utilization deeply of it.

A Review] The oncogene expression and growth of leukemic cells could be inhibited by antisense oligonucleotide. The selection of target genes is the key step in the research of antisense oligonucleotide on leukemia. The article would review the status and prospect of some target genes of leukemia in the investigation of antisense oligonucleotide.

AIM: To investigate whether antisense oligodeoxynucleotides of hTERT?bcl-2 and c-myc could enhance the sensitivity of leukemia cell K562 to cisplatin. METHODS: The inhibiting effects of cisplatin and cisplatin plus antisense oligodeoxynucleotide on K562 cells were determined by MTT. RESULTS: The inhibiting rate of 20 ?mol/L cisplatin to K562 cell is 17.17%?1.36% and it becomes 25.41%?1.77% ,26.18%?1.43% and 28.29%?1、05%, respectively, as combinated with antisense oligodeoxynucleotide of hTERT?bcl-2 or c-myc. CONCLUSION: These results indicated that antisense oligodeoxynucleotides of hTERT?bcl-2 and c-myc enhanced efficacy of cisplatin in K562 leukemic cells.

AIM: To observe the effect of antisense oligodeoxynucleotide of human telomerase reverse transcriptase(hTERT) on the telomerase activity in Jurkat cells and its mechanism. METHODS: The telomerase activity was detected by TRAP. hTERT protein and mRNA expressions were detected by flowcytometry and RT-PCR. RESULTS: The absorbency of the cells ( A values) was used to represent the telomerase activity. At 48 h and 72 h, the A values of the Jurkat cells treated with antisense oligodeoxynucleotide of hTERT decreased to 0.351?0.051 and 0.238?0.024, respectively, compared to the A value of 0.492?0.051 in the control cells without treatment ( P

AIM: To explore the effect of human telomerase reverse transcriptase (hTERT) gene antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) on cisplatin-induced apoptosis in cultured primary acute lymphoblastic leukemia (ALL) cells. METHODS: The expression levels of hTERT protein were detected by immunofluorescence using fluoresce isothiocyanate (FITC) lable. Cell surviving fraction was determined using the trypan blue dye exclusion assay. Apoptosis was detected by morphological observation and flow cytomertric cell cycle analysis. RESULTS: The expression of hTERT protein was inhibited after treated by hTERT AS PS-ODN. Treatment with cisplatin after 24 h of exposure to AS PS-ODN had significantly reduced the number of viable ALL cells. However,there was no difference on ALL cells survival between sense oligodeoxynucleotide (S PS-ODN) /CDDP combination and CDDP-treated cells alone. In morphological observation of apoptotic cells using Hoechst 33258 and PI double staining techniques,cells displayed classic apoptotic changes treated with CDDP or CDDP combined with hTERT AS PS-ODN or S PS-ODN at 48 h. Apoptotic rates of cells added CDDP and AS PS-ODN were higher than that of cells added CDDP only ( P 0.05). CONCLUSION: hTERT AS PS-ODN inhibited the expression of hTERT protein and increased the CDDP-induced apoptosis in primer acute lymphoblastic leukemic cells.

AIM: To explore the effect of hTERT antisense phosphorothioate oligodeoxynucleotide (ASODN) on apoptosis induced by chemotherapeutic drugs in Jurkat cell lines. METHODS: Cell viability was determined using the trypan blue dye exclusion assay. Apoptosis was detected by morphological observation, DNA gel electrophoresis and flow cytometry analysis. RESULTS: The survival rates of Jurkat cells cultured with daunorubicin, vincristin, and etoposide, respectively were similar with that cultured with those chemotherapic drugs plus hTERT ASODN. The survival rates of Jurkat cells cultured with cis-diamminedichicloroplatinum(DDP) added 24 hours later were higher than that cultured with hTERT ASODN and DDP added 24 hours later. The survival rates of Jurkat cells cultured with DDP were similar with that cultured with hTERT SODN and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells displayed classic apoptotic changes treated with DDP or DDP combined with hTERT ASODN or SODN at 48 hours. Agarose gel electrophoresis of genomic DNA from Jurkat cells treated with ASODN and DDP combination for 48 hours showed typical DNA “ladder”. Neither the DNA from Jurkat cells treated with SODN plus DDP nor the DNA from the cells treated with DDP alone showed ladder pattern. Apoptosis rates of Jurkat cells treated with DDP for 48 hours after 24 hours of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic Jurkat cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively. CONCLUSION: The hTERT ASODN complementary to the translation initiation region of hTERT mRNA enhanced DDP-induced apoptosis in Jurkat cells.