0.05).A lot of Nissl bodies in the cell body were seen in induced cells shown by thionine-eosin staining.Conclusion The bone-marrow-derived MSCs can differentiate into neurocytes by either chemical or biological inducers.

Objective To explore the application of cognitive intervention and drug intervention in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 86 patients with chronic obstructive pulmonary disease(COPD)admitted from February 2016 to July 2016 were included in this study.The patients were divided into experimental group and control group according to the order of admission.43 patients in the control group received routine measures,In the experimental group,43 patients received cognitive intervention and drug intervention,compared the clinical treatment effect and treatment compliance of the two groups after intervention.Results The compliance rate of disease cognition,smoking,medication,pulmonary function,family oxygen therapy,respiration exercise,rehabilitation therapy,nutritional support and influenza vaccination were significantly better in the experimental group than in the control group(P<0.05)(1.2±0.7)times,the number of acute attack was(2.0±0.8)times and the quality of life was(4.4±0.9)points,which was significantly better than that of the control group(P<0.05).Conclusion Cognitive intervention and drug intervention have a positive effect on the treatment compliance of patients with chronic obstructive pulmonary disease,improve the quality of life of patients,and have significant clinical application value.

Objective To investigate the morphologic characteristics of neuron-like cells induced from the rat mesenchymal stem cells(MSCs). Methods The rat MSCs from bone marrow were cultured by being adhered to the culture dish.The thirdgeneration of the MSCs were induced by the neonate rat brain homogenate supernatant for 48 hours.The morphological characteristics and the ultrastructures of both non-induced and induced MSCs were observed under the inverted microscope and the electron microscope.The property of the induced cells was identified by using immunocytochemical method. Results Under the inverted microscope MSCs showed spindle or polygon-shaped cell bodies and the nuclei with one or two nucleoli were located in the middle of the cells.After the inducement the cells appeared neuron-like with axonlike and dendrite-like processes.The neuron-like cells were neuron special endolase(NSE),neurofilament protein(NF) positive and glial filbrillary acidic protein(GFAP) negative by irnmunocytochemical staining.Under the electron microscope the MSCs had plentiful cytoplasm and organelles and had an obvious nuclei containing a nucleoli.There were a lot of microvilli on the surfaces of the MSCs.The neuron-like cells had plentiful cytoplasm and irregular nuclei with one to three nucleoli.There were also plenty of microvilli on the cell surfaces.Conclusion MSCs are one kind of multipotent stem cells and can differentiate neurons with matured organelles induced by the neonate rat brain homogenate supernatant.

Objective To compare the clinical efficacy of intralesional betamethasone versus triamcinolone acetonide acetate in the treatment of active alopecia areata. Methods A total of 160 patients with active alopecia areata were divided into two groups, test group (n = 100) treated with intralesional betamethasone, and control group (n = 60) treated with intralesional triamcinolone acetonide. Both injections were given once every 3 weeks for 12 consecutive weeks. Results After 12-week treatment, the cure rate, response rate, and total response rate were 60.0%, 32.0% and 92.0% in the test group, respectively, compared to 41.7%, 31.67% and 73.3% in the control group, respectively. A significant increase was observed in the cure rate and response rate in the test group compared with the control group (χ2 = 10.25, 5.06, P < 0.01 and 0.05). During the treatment course, 8 (8%) patients in the test group and 9 (15%) patients in the control group developed localized atrophy of the scalp; 8 (8%) patients in the test group and 3 (5%) patients in the control group developed localized folliculitis; no significant difference was observed between the two groups in the occurrence of adverse reactions (P> 0.05). Conclusion Intralesional use of compound betamethasone injection has a notable therapeutic effect on alopecia areata.

Objective To research the effect of GGCX gene on MMP13 in rabbit osteoarthritis cartilage cells and investigate its effect on osteoarthritis cartilage degeneration.Methods Six Japanese big ear rabbits weighted (2.0±0.2)kg were randomly di vided into three groups,each group seted a rabbit as control.Anterior cruciate ligament transection method was used to build osteoarthritis cartilage degeneration model at second,forth,sixth week.Articular cartilage was separated successfully after the model were built,cartilage cells were divided from articular cartilage and cultured in 6-well cell culture plate.Cartilage cells were divided into blank group,negative control group and transfection group.PCR method and Western blot were conducted to detect GGCX and MMP13 expressed at the level of mRNA and protein.Results Compare with blank group and negative control group,the expressin level of GGCX incresed,while the MMP13 expression level dcresed(P＜0.05).Conclusion Over expression of GGCX gene can ob viously decrease the expression of MMP13,it provide experimental basis for osteoarthritis of the in vitro gene therapy.

Objective To compare the efficacy and safety of lauromacrogol injection sclerotherapy with ethanol injection sclerotherapy in treating simple liver cysts. Methods A total of 166 patients with simple liver cyst were randomly divided into the lauromacrogol group (study group, n=86) and the absolute alcohol group (control group, n=80). Under ultrasonographic guidance, puncture aspiration of liver cyst was carried out in all patients, which was followed by injection of lauromacrogol for patients in the study group or injection of ethanol for patients in the control group. The therapeutic effect and the side-effect were evaluated. The results were compared between the two groups. Results No serious complications such as bleeding or infection occurred in both groups. During the therapeutic course , 45 patients (56.3%) in the control group felt pain to some degree and 23 patients (28.8%) developed drunk-like symptoms, while no patient in the study group felt any obvious discomfort. One week after sclerotherapy , 20 patients (25%) in the control group complained of distending pain on the right upper abdomen, while only 9 patients (10.5%) in the study group complained of pain, and the difference was statistically significant (χ2= 6.073, P < 0.05). Six months after the treatment, the cure rate of the study group and the control group was 95.7%and 93.5%respectively, and the difference between the two groups was no significant (P > 0.05). Conclusion For the treatment of liver cysts, lauromacrogol injection is safe and effective. Therefore, this technique should be recommended in clinical practice.

Postgraduate education must be systematic and explicit.In recent years,the cultivation of domestic tertiary education for postgraduate students conducted a multi-disciplinary approach.This article give comment on the current postgraduate training objectives,methods,curriculum and training process,training instructors,and puts forward some problems to be discussed in depth.

Objective To investigate the association of HLA class Ⅱ genes and vitiligo in the eastern China Han nationality. Methods Ninety-eight patients with vitiligo and 150 healthy controls were studied for HLA-DRB1, DQA1 and DQB1 locus alleles by PCR-SSOP typing. Results The frequency of HLA-DQA1*03 increased significantly (Pc = 0.008) and DQA1*05 decreased significantly (Pc = 0.016) in the patients with vitiligo. Conclusions The results suggest that there exists a correlation between HLA class Ⅱ genes and vitiligo, and DQA1*03 allele may be a susceptible gene or have a close linkage with susceptible genes, while DQA1*05 allele may be a protective gene in the eastern China Han nationality.

OBJECTIVETo cultivate human mesenchymal stem cells (MSC) derived from fetal bone marrow and examine their pluripotentiality.

METHODSBone marrow mononuclear cells from human fetal femur were collected by Percoll gradient centrifugation. The low density cells including MSCs were cultivated and expanded in MSCGM media. The characteristics of the multipotent MSCs were observed by implanting them into nude mice for 4 weeks.

RESULTSHuman fetal marrow MSCs were successfully cultivated and differentiated in vivo into many kinds of tissues such as bone, cartilage, adipose, skeletal muscle and tendon-like tissue.

CONCLUSIONHuman fetal marrow MSCs were multipotent stem cells.

Animals ; Bone Marrow Cells ; cytology ; Cell Culture Techniques ; methods ; Cell Differentiation ; Cell Division ; Cell Transplantation ; methods ; Cells, Cultured ; Humans ; Mesoderm ; cytology ; Mice ; Mice, Nude ; Stem Cells ; cytology ; Transplantation, Heterologous

Objective To investigate the effects of Baicalin on intestinal mucosal injury in rats with partial common bile duct ligation (PCBDL).Methods Forty male Wistar rats were randomly divided into 4 groups equally:sham operation,PCBDL,PCBDL1 and PCBDL2.Rats in PCBDL,PCBDL1 and PCBDL2 groups were subjected to partial common bile duct ligation.Baicalin [80 mg/(kg · d)] was fed in PCBDL1 group (for 2 weeks) and PCBDL2 group (for 3 weeks),while for other groups,9 g/L saline in the same volume was fed.Ileum mucosa were prepared for microscopic examination.The intestinal mucosal injury in rats was observed and scored.The level of NF-κB mRNA expression by Fluorescent in Situ Hybridization,and the level of NF-κB protein were determined by immunohistochemistry.Results 1.Compared with PCBDL group (3.2 ± 0.5),the pathological severity scores of intestinal mucosa significantly declined (F =21.120,P ＜ 0.01) in PCBDL1 group (1.9 ± 0.2) and PCBDL2 group (1.5 ± 0.3).2.Compared with sham operation group(0.066 ± 0.006),PCBDL1 group (0.107 ± 0.011),and PCBDL2 group (0.098 ± 0.010),NF-κB expression in PCBDL group (0.155 ± 0.012) presented significantly up-regulation (F =76.8,P ＜ 0.01).3.Compared with sham operation,PCBDL1 group,and PCBDL2 group,the positive expression rates of NF-κB mRNA of intestinal mucosal epithelium in PCBDL group significantly increased.Conclusions It is suggested that Baicalin exert protective effects on the intestinal mucosal injury in rats with PCBDL,partially by inhibiting NF-κB mRNA,down-regulating NF-κB protein expression of intestinal mucosal epithelial cells.