1.Regulatory Action of BushenqingreFang on Apoptosis of Nephridial Tissue in Mice with Lupus Nephritis Caused by cGVHD
Yangrong CHEN ; Wenying REN ; Ming JIANG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
Objective: To study the regulatory action of BushenqingreFang on Fas/FasL in the cells of nephridial tissue in mice with lupus nephritis (LN) caused by chronic graft-versus-host disease (cGVHD). Methods: To observe the apoptosis in nephridial tissue by TUNEL and to measure the Fas/FasL genetic transcription and their protein expression by immunohistochemistry, western blot and RT-PCR techniques. Results: BushenqingreFang increased the positive score of TUNEL in nephridial tissue and up regulated mRNA expression and protein levels of Fas and FasL. Conclusion: The action of BushenqingreFang on LN may be conducted by its up regulation of Fas and FasL and its promotion of apoptosis.
2.Therapeutic effect of simvastatin combined cyclic adenosine monophosphate on chronic heart failure and levels of serum-related factors
Wenying SUN ; Shuling DONG ; Zhiyan REN
Chinese Journal of cardiovascular Rehabilitation Medicine 2014;23(3):324-327
Objective:To explore the clinical therapeutic effect of simvastatin combined cyclic adenosine monophos-phate on coronary heart disease (CHD)with chronic heart failure (CHF)and levels of serum-related factors.Meth-ods:A total of 78 CHD patients with CHF hospitalized in our hospital from Jan 2011 to Jan 2012 were selected. They were randomly and equally divided into simvastatin group and combined treatment group using number table, both groups received routine treatment,simvastatin group received simvastatin in addition, while combined treat-ment group received cyclic adenosine monophosphate based on simvastatin group.Therapeutic effect,changes of cardiac function indexes and serum factor levels were compared between two groups before and after treatment.Re-sults:The total effective rate of combined treatment group (92.31%)was significantly higher than that of simvasta-tin group (71.79%),P<0.05;compared with before treatment and simvastatin after treatment,there were signifi-cant rise in left ventricular ejection fraction [LVEF,(33.54±3.34)%,(43.41±3.23)% vs.(55.21±3.45)%] and transmitral early/late diastolic peak flow velocity [E/A,(0.63±0.11),(0.70±0.15)vs.(1.01±0.21)],and significant reductions in levels of brain natriuretic peptide [BNP,(536.74±21.41)ng/ml,(117.23±11.57)ng/ml vs.(78.20±10.92)ng/ml]and C reactive protein [CRP,(24.00±2.34)mg/L,(17.01±1.09)mg/L vs.(8.28± 0.81)mg/L]in combined treatment group,P<0.05~<0.01.Conclusion:Simvastatin combined cyclic adenosine monophosphate possess significant therapeutic effect on chronic heart failure of coronary heart disease,it can signif-icantly improve cardiac function and serum factor levels in CHD patients with chronic heart failure,and possess good clinical application value.
3.Association study between carotid artery bruit and carotid stenosis in cerebral arterial thrombosis
Yanfang YANG ; Lijun REN ; Pingfan WANG ; Chunhong LIU ; Wenying ZHANG ; Qiang HAO ; Ying LIU
Chinese Journal of Postgraduates of Medicine 2010;33(34):20-22
Objective To evaluate the value of carotid artery bruit in predicting carotid stenosis in patients with cerebral arterial thrombosis. Methods Two hundred cerebral arterial thrombosis patients were divided into bruit group and no bruit group according to carotid artery auscultation,with 100 cases in each group. The patients with cardiac murmur, vein sound and thyroid noise were excluded from bruit group. The examination of carotid artery auscultation (transcranial Doppler sonography ) was managed in all the patients,and digital subtraction angiography (DSA) about aortic arch and the whole brain were perfomed. The carotid stenosis rate, site, degree were compared and the relationship between carotid bruit grade and carotid stenosis degree were evaluated. Results The carotid stenosis rate in bruit group [67% (67/100)] was higher than that in no bruit group [29%(29/100)](P< 0.01 ). There was significant difference in the carotid stenosis site and degree between two groups (P <0.05). Carotid bruit were significantly associated with stenosis near carotid bifurcation or serious stenosis (P < 0.05). The carotid bruit grade was positively related with the carotid stenosis degree (P < 0.05 ). Conclusions Carotid bruit is associated with site and degree of carotid stenosis. Carotid bruit is valuable in screening for carotid stenosis in patients with cerebral arterial thrombosis.
4.Establishment of risk warning model for surgical site infection
Wenying HE ; Yuhong DENG ; Xin LIU ; Weiguang LI ; Anhua WU ; Nan REN ; Lijuan XIONG ; Lili DING ; Hui HAN ; Zhong WANG
Chinese Journal of Infection Control 2017;16(6):497-501
Objective To establish a risk warning model for surgical site infection(SSI), provide support for screening high risk population and finding suspected cases.Methods Clinical data of 5 067 patients who underwent abdominal surgery in 6 domestic hospitals from January 2013 to December 2015 were collected retrospectively, all cases were randomly divided into modeling group and validation group according to a 6:4 ratio, warning model was established by employing logistic regression, the area under the receiver operating characteristic curve (AUC) was used to evaluate discriminant ability of evaluation model, the maximum Youden index was as the optimum cut-off point.Results For the warning model of high-risk patients, AUC was 0.823, sensitivity and specificity were 78.81% and 74.33% respectively, positive predictive value and negative predictive value were 19.67% and 97.78% respectively.For the discriminant model of suspected infection cases, AUC was 0.978, sensitivity and specificity were 93.38% and 95.62% respectively, positive predictive value and negative predictive value were 62.95% and 99.45% respectively.Conclusion The early-warning model established in this study has better discrimination ability, which can provide a reference for the development of early warning and discrimination of healthcare-associated infection information system.
5.Experience of children with acute leukemia about acupoint therapy during chemotherapy: a qualitative research
Anwei XIE ; Yuying CHAN ; Wenying YAO ; Yueye XU ; Liqin REN
Chinese Journal of Practical Nursing 2017;33(30):2355-2357
Objective To explore the lived experience of children with acute leukemia about acupoint therapy during chemotherapy, so as to provide the theoretical and practice evidence for development of more scientific nursing measures. Methods Totally 14 children with acute leukemia participated in half-structured interviews regarding their experience of acupoint therapy.The NVivo 10.0 software was used to supervise, excavate and seek data.The data were analyzed by the Co1aizzi phenomenological methodology. Results Five themes were extracted including benefit sense,a sense of accomplishment of self-fulfilling, a sense of attachment to medical, sigh with emotion the magic of traditional Chinese medicine and bearable mild discomfort. Conclusions The experience of children with acute leukemiais positive feelings for the most part, nursing staff should listen to them and give corresponding nursing according to specific circumstances in order to improve the quality of nursing.
6.Consensus on diagnosis on congenital intestinal aganglionosis.
Wen ZHANG ; Haiyan WU ; Hui LI ; Huibo AN ; Wenying ZHAO ; Wenping YANG ; Guangsheng CHEN ; Jing TAO ; Weijian CHEN ; Yubo REN ; Zheng AN ; Libing FU ; Lejian HE ; null
Chinese Journal of Pathology 2016;45(3):149-152
7.Correlation between HLA-DQA1 allele and anaphylactoid purpura in juvenile Hans residing in Inner Mongolia.
Shaomin REN ; Linhu TONG ; Gaowa XILIN ; Junqing SUI ; Zhongxian GAO ; Wenying YAN ; Weicai LI
Chinese Journal of Medical Genetics 2002;19(1):58-60
OBJECTIVETo analyze the genetic susceptibility of HLA-DQA1 allele to anaphylactoid purpura(AP)and its association with the clinical features in juvenile Hans residing in Inner Mongolia.
METHODSSeventy children with AP and ninety normal controls of Hans in Inner Mongolia were subjected to HLA-DQA1 genotyping with the use of polymerase chain reaction-sequence specific primer (PCR-SSP) technique.
RESULTS(1) The gene frequency of HLA-DQA1*0301 of AP group (33.4%) was significantly higher than that (10.6%) of control group (chi square=21.899, P<0.01). On the other hand, the gene frequencies of HLA-DQA1*0302 were 6.7% and 19% in the AP group and the control group respectively; a significant difference between them was seen (chi square=9.786, P<0.01); (2)The gene frequencies of both DQA1*0301 and DQA1*0302 in the cutaneous purpura simplex cases and the controls were not significantly different (P>0.05). The gene frequencies of DQA1*0301 of the cutaneous purpura cases associated with gastrointestinal, joint and renal impairment were 26.7%, 28.5% and 29.3% respectively, which were higher than that of the control group (10.6%); the differences were statistically significant (P<0.01, 0.01, 0.01; respectively). The gene frequencies of HLA-DQA1*0302(3.9%, 5.7% and 9.6%) for the cutaneous purpura cases associated with gastrointestinal, joint and renal impairment were significantly lower than that (19%) of the controls except renal impairment(P<0.01, 0.01, respectively).
CONCLUSIONThe allele of HLA-DQA1*0301 was probably a susceptible gene while HLA-DQA1*0302 was the protective one in AP of the children who were Han inhabitants in Inner Mongolia. The results of this study also revealed that patients with the allele of HLA-DQA1*0301 tended to involve gastrointestinal, joint and renal impairment.
Adolescent ; Asian Continental Ancestry Group ; genetics ; Child ; Child, Preschool ; China ; Female ; Gastrointestinal Diseases ; complications ; genetics ; Gene Frequency ; Genetic Predisposition to Disease ; HLA-DQ Antigens ; genetics ; HLA-DQ alpha-Chains ; Humans ; Joint Diseases ; complications ; genetics ; Male ; Purpura, Schoenlein-Henoch ; complications ; genetics ; Renal Insufficiency ; complications ; genetics
8.Standardized Operational Protocol for Human Brain Banking in China.
Wenying QIU ; Hanlin ZHANG ; Aimin BAO ; Keqing ZHU ; Yue HUANG ; Xiaoxin YAN ; Jing ZHANG ; Chunjiu ZHONG ; Yong SHEN ; Jiangning ZHOU ; Xiaoying ZHENG ; Liwei ZHANG ; Yousheng SHU ; Beisha TANG ; Zhenxin ZHANG ; Gang WANG ; Ren ZHOU ; Bing SUN ; Changlin GONG ; Shumin DUAN ; Chao MA
Neuroscience Bulletin 2019;35(2):270-276
Brain
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pathology
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China
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Humans
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Organ Preservation
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standards
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Tissue Banks
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ethics
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standards