Objective:To study the effect of sufentanil combined with dezocine on the PCIA after abdominal operation. Methods:Totally 110 patients undergoing abdominal operation were randomly divided into the experimental group and the control group with 55 cases in each. The two groups were both treated with electronic analgesia pump for the PCIA after the operation. The control group was given dezocine 0. 3 mg·kg-1 ,and the experimental group was given sufentanil injections 0. 1 g·kg-1 plus dezocine 0. 3 mg·kg-1 . The VAS score,adverse drug reactions,Ramesay scores,press number of patient controlled analgesia,exhaust time,recovery time of bowel sounds and bowel audio rate index in the two groups were compared to evaluate the effect of PCIA. Results:All the observed in-dices in the experimental group were significantly better than those in the control group(P<0. 01). The incidence of adverse reactions in the experimental group was significantly higher than that in the control group(P<0. 01). Conclusion:Sufentanil combined with dezocine for the PCIA after abdominal operation shows significantly analgesic and sedative effects,and can notably improve the clinical symptoms,which is worthy of clinical application.

Objective To explain the material foundation of anti-inflammatory and analgesic effects of Jinxuan Zhike Xunxi San. Methods The foot swelling experiments in rat and xylene-induced ear edema experiments in mouse were adopted to observe the anti-inflammatory effects of the material extracted and purified from Jinxuan Zhike Xunxi San. The hot plate-induced pain experiment for studying the analgesic effect was established. Results The material of supercritical CO2 extract, saponins and flavonoids extract and minerals in Jinxuan Zhike Xunxi San had a inhibiting effect on egg-induced foot swelling in rat, saponins and flavonoids extract had a distinct inhibiting effect on xylene-induced ear edema in mouse. Every extract had obvious analgesic effects on hot plate-induced pain and significantly increased the threshold of pain. Conclusion The active ingredient groups of Jinxuan Zhike Xunxi San are the material of supercritical CO2 extract, saponins and flavonoids extract and minerals.

Objective To establish a loop-mediated isothermal amplification (LAMP) assay for rapid detection of the West Nile virus (WNV).Methods WNV genome (position nt 1 021 to nt 1 240) was synthesized by a PCR-based gene synthesis method.The synthetic fragments included 6 pairs of LAMP primer recognizing 8 primer sites of WNV genome.The LAMP gene amplification was carried out using a real-time PCR system at 63℃ for 60 min,then the amplification was terminated at 80℃ after 2 min.The amplification products were observed by agarose gel electrophoresis.The sensitivity and specificity of LAMP assay were compared with those of conventional PCR.Results The LAMP assay took less than 20 min,and the amplification product took on a ladder-like electrophoresis pattern.The sensitivity of LAMP assay was 10-fold higher than that of conventional PCR,and the detection limit of LAMP was 9.23 copies/?l.The specificity of WNV-specific LAMP assay was demonstrated by the negative amplification results from dengue virus and Japanese encephalitis virus,both were closely related members of the Flavivirus family.Conclusion LAMP assay is rapid,cost-effective,highly sensitive and specific in detecting genes of interest,and is of great significance for WNV surveillance,especially for grass root units and on-sport surveillance.

Objective To apply James Sheehy myringoplasty for treatment of large perforation without residual rim and to observe the efficacy.Methods Between January 2001 and June 2003, 60 patients with large perforation without residual rim had underwent James Sheehy myringoplasty, in which 36 were males and 24 were females, aged from 16 to 64 years and with an average of 45.23 years. Preoperative air-bone gap was 5 to 45 dB, averaged 28.5 dB. Fellow-up was done more than 6 months on 54 patients.The key technic: (1) a vascular skin flap was made on the posterior canal wall and the anterior canal wall skin was excised. (2) canaloplasty was performed until the whole tympanic sulcus can be seen under one microscope view. (3) if necessary, the ossicular chain was explored. (4) overlay technic was used to repair perforation with temporal fascia. (5) the free anterior skin and the posterior canal vascular flap were replaced.Results 52 patients were healed in one stage. In 2 patient, two needle tip-like perforations appeared in the center of the grafting tympanic membrane 3 months after surgery, which were closed after gelatin sponge patching. The shape of the tympanic membrane looked as normal. Symptoms of temporomandabular joint appeared in 6 patients and disappeared 3 days later. Lateral displacement of the graft happened in 2 patients.Conclusion James Sheehy myringoplasty is a reliable technique for treatment of large perforation without residual rim, with high success rate, less postoperative response and good shape of new tympanic membrane.

Objective To make a clinical and mycological analysis of tinea capitis in Guangzhou region. Methods A retrospective analysis was performed on 241 cases of tinea capitis collected from Feb, 1997 to Aug, 2010 in the Department of Dermatology, Sun Yet-sen Memorial Hospital. Results Among the 241 cases, 179 (74.27%) were tinea alba, 34 (14.11%) tinea kerion, 28 (11.62%) black dot ringworm, and no favus was observed. The dominant pathogenic fungi in decreasing order were Microsporum canis (182,80.89%), Trichophyton violaceum (25, 11.11%), Trichophyton mentagrophytes (10, 4.44%), Trichophyton tonsurans (3, 1.33%), Trichophyton rubrum (2, 0.89%), Microsporum gypseum (2, 0.89%) and Trichophyton verrucosum (1, 0.44%). Children were the main population (39.00%) suffering from tinea capitis. Conclusions In Guangzhou region, tinea alba is the most common type of tinea capitis, Microsporum canis is the main causative pathogen, and children are the predominate population affected by tinea capitis.

Objective To investigate the serum and urine levels of neutrophil gelatinase‐associated lipocalin(NGAL) in patients with primary hypertension and related clinical significance .Methods A total of 107 patients with primary hypertension and 100 healthy subjects were enrolled ,and detected for serum and urine levels of NGAL and urinary micro‐albumin .Results Serum and u‐rine levels of NGAL in hypertension patients were (1 .98 ± 1 .49) and (1 .29 ± 0 .54)μg/L ,higher than the (0 .69 ± 1 .02) and (0 .55 ± 0 .35)μg/L in healthy subjects(P< 0 .05) .Urinary micro‐albumin level in hypertension patients was also higher than healthy subjects (P<0 .05) .Conclusion Serum and urine levels of NGAL in patients with primary hypertension could be signifi‐cantly increased ,which might be with important clinical significance in diagnosis ,treatment and prognosis of hypertensive nephropa‐thy .

Objective To evaluate the effectiveness and safety on once-daily (OD) insulin detemir (IDet) in Chinese patients with type 2 diabetes mellitus (T2DM) who were treated with different types or combinations of oral anti-diabetic drugs (OADs).Methods The SOLVETM study was a 24-week observational study on the initiation of IDet OD in T2DM patients with uncontrolled hyperglycemia on diet,exercise,and one or more OADs.Subjects were grouped based on the numbers of OADs taken before (＞ 2-OAD,2-OAD,and 1-OAD groups).Efficacy and safety endpoints were evaluated and compared in different groups.Results This study includes 3 272 patients,among them 464 (14.2％) were treated with more than 2OADs,1511 (46.2％) with 2OADs,and 1 218 (37.2％) with 1OAD before the study.The mean glycosylated hemoglobin A1c (HbA1c) was 8.4％,8.3％,8.4％ at baseline,and 7.3％,7.2％,7.1％ at the end of 24-week in each 3 groups (all P ＜0.001 vs.baseline values).The HbA1c reductions were not statistically significant different among groups.Body weight tended to decrease in patients from all groups,however,only that in the 2-OAD group reached statistically significance.No major hypoglycaemia events were reported.However,the overall minor hypoglycaemia rate in the 2-OAD group was higher at the end of the study than that at baseline (P ＜ 0.05).No differences in the rate of nocturnal minor hypoglycaemia were observed in all groups after IDet treatment.Conclusion Initiation of IDet OD was effective and well-tolerated in Chinese patients with T2DM whose glycemia was poorly controlled on OADs irrespective of the number of OADs taken before.(registration number NCT00825643)

Objective To evaluate the influences of laryngeal mask airway(LMA) combined with epidural anesthesia on hemodynamics in hypertensive patients.Methods 72 gynecological patients with stage Ⅰ to Ⅱ hypertension were randomly divided into four groups(n=18 for each):general anesthesia with tracheal intubation(group G) or LMA(group L),combination of epidural anesthesia and general anesthesia with tracheal intubation(group GE) or LMA(group LE).BP,HR,ECG,SpO2 were monitored in different time.Intraoperative awareness,the time of extubation or LMA removal and anesthetic dosages were recorded.Results During insertion of the tube or LMA,SBP,DBP,HR were significantly higher than those before anesthesia in group G and GE(P

Objective To study the dose rate distribution on cultured cell plane and establish a reference dose rate table of in vitro cell line ~(125)I seed irradiator.Methods Thermoluminescence dosimetry(TLD) was used to measure the irradiation dose rate of a single 6711 model(~(125) I) seed with apparent activity of 10.323 MBq in water at point P.Meanwhile,the theoretic value of the irradiation dose rate at point P was calculated with theoretic formula.The difference between the calculated and observed values within 10% was set as standard to analyze the accuracy of the measurement.The irradiation dose rate of a single 6711 model(~(125) I) seed was measured in 1mm-thick polystyrene + water medium at point P.The value was applied to differential or non-differential proof along with the value from water medium to study the effect of 1 mm thick polystyrene on distribution of irradiation dose in water.Finally,by simulating the(~(125) I) seed plane irradiator with nine(~(125) I) seeds,the distribution table of irradiation dose rate on the cultured cell plane was calculated with theoretical formula.Results The observed value(n= 10) of irradiation dose rate with one(~(125) I) seed in water at point P was(0.359?0.023)cGy/h and the calculated value was 0.347cGy/h,the difference was within 10%.The observed value(n=10) of irradiation dose of one(~(125) I) seed in 1mm-thick polystyrene + water medium at point P was (0.350?0.027)cGy/h,which showed no statistical difference from the observed value in water under differential and non-differential proof.The reference table on dose rate distribution for cells exposed to(~(125) I) seed irradiation in vitro was developed.Conclusions 1mm-thick polystyrene gives no significant effect on irradiation dose rate distribution from(~(125) I) seeds in water.A reference table on the dose rate distribution for cells exposed to(~(125) I) seed irradiation in vitro has been developed,which can be used to determine an optimal irradiating strategy for future work.

OBJECTIVETo assess the exposure level of total N-nitroso compounds (TNOCs) in the residents of high- and low-risk areas for esophageal cancer in southern China.

METHODSSamples of duplicate plate diets and 12 hr overnight urine were collected from 120 male adults in each of the 2 areas, a high-risk area (Nanao county) and a low-risk area (Lufeng county) for esophageal cancer. The 240 male healthy subjects (35 - 64 years old) were selected by a 3-stage random cluster sampling procedure. Levels of TNOC, N-nitrosamino acids (NAAs) and volatile N-nitroso compounds (VNOC) in the samples were measured by Thermo Energy Analyzer.

RESULTSThe detectable rate (95%) of diet TNOC, daily dietary TNOC intake (4.25 +/- 0.84) micromol/day, 12-hr urinary TNOC excretion levels (1.76 +/- 0.23 ng/12 h) and daily dietary intake of VNOC (266 +/- 31.2 microg/day) in the high-risk area were all significantly higher than those of the low-risk area. Oesophageal cancer mortality rates were positively and significantly associated with daily dietary TNOC intake and 12-hr urinary TNOC excretion. Urinary NAAs excretion levels were not different in the two areas.

CONCLUSIONThe results suggest that TNOCs may be implicated in the etiology of esophageal cancer in southern China.

Adult ; China ; Esophageal Neoplasms ; etiology ; mortality ; Humans ; Male ; Middle Aged ; Nitroso Compounds ; administration & dosage ; adverse effects ; urine