OBJECTIVE:To establish the quality standards for Fufang ningshen granule. METHODS:TLC was used for the qualitative identification of Radix angelicae and White paenoy;HPLC was adopted for the contents determination of spinosin and verbascoside:the column was Kromasil C18 with the mobile phase of acetonitrile-0.1% acetic acid(16∶84,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temerpature was 30 ℃. RESULTS:The TLC spots of R. angelicae and W. pae-noy were clear with good separation,negative control without interference. The linear range was 0.055 44-0.277 2μg for spinosin(r=0.999 4)and 0.055 98-0.279 9 μg for verbascoside(r=0.999 5);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 95.62%-100.53%(RSD=1.77%,n=9) and 95.63%-102.57%(RSD=2.74%,n=9). CONCLU-SIONS:The standard can be used for the quality control of Fufang ningshen granule.

OBJECTIVETo study the chemical constituents from Corallodiscus kingianus.

METHODThe column chromatographic techniques were applied to isolate constituents. A combination of ESI-MS and NMR spectroscopy was used to identify structures.

RESULTSix compounds were isolated from the acetone extract of this plant, and the structures of them have been identified as castanopsol (1) , 3beta-hydroxy-9 (11), 12-oleanadien-28-oic acid (2), 2,3,7-trihydroxy-6-oxo-1,3,5 (10), 7-tetraene-24-nor-frie-delane-29-oic acid methylester (3), 3-epicyclomusalenol (4), palmitinic acid (5) and stearic acid (6).

CONCLUSIONCompounds 1-6 were isolated for the first time from Gesneriaceae.

Drugs, Chinese Herbal ; analysis ; Magnoliopsida ; chemistry ; Triterpenes ; analysis

Objective To explore the differential diagnosis between gallbladder polypoid le-sions and gallbladder benign tumor.Methods 156 patients with gallbladder polypoid lesions were examined through the different imaging method,and the results were compare with the pathological findings.Results Compared with the pathological diagnosis,the ultrasound imaging (χ2=0.188, P >0.188),enhanced CT (χ2= 2.308,P >0.05)and MRI (χ2=7.505,P >7.505)showed no significant differences in detection rate.Contrast-enhanced ultrasound was significantly better than enhanced CT (χ2=4.769,P <4.769)and MRI (χ2=14.467,P <0.05).Conclusion Contrast-enhanced ultrasound can carefully reflect the gallbladder polypoid lesions of peripheral vascular and morphological characteristics.Because its highest detection rate,it is suggested as the preferred method for the diagnosis of gallbladder benign tumor.

Objective To explore the differential diagnosis between gallbladder polypoid le-sions and gallbladder benign tumor.Methods 156 patients with gallbladder polypoid lesions were examined through the different imaging method,and the results were compare with the pathological findings.Results Compared with the pathological diagnosis,the ultrasound imaging (χ2=0.188, P >0.188),enhanced CT (χ2= 2.308,P >0.05)and MRI (χ2=7.505,P >7.505)showed no significant differences in detection rate.Contrast-enhanced ultrasound was significantly better than enhanced CT (χ2=4.769,P <4.769)and MRI (χ2=14.467,P <0.05).Conclusion Contrast-enhanced ultrasound can carefully reflect the gallbladder polypoid lesions of peripheral vascular and morphological characteristics.Because its highest detection rate,it is suggested as the preferred method for the diagnosis of gallbladder benign tumor.

Objective:To explore the value of FibroScan in liver grafts from brain-dead donors (DBD) prior to liver transplantation (LT).Methods:Liver grafts from 52 DBD were examined using ultrasound and FibroScan before LT. The causes of death were cerebral hemorrhage ( n=25), brain trauma ( n=21) and ischemic-hypoxic cerebropathy ( n=6). Blood samples were tested before LT and a biopsy was performed pre- or intra-operation for determining pathology. The diagnostic accuracy of FibroScan results was compared with that of pathological examinations. The latter is a gold standard for evaluating liver grafts. The eligible donors were grouped by stage of liver fibrosis (F0-F4) and steatosis (S0-S3) based upon Kleiner's scoring system of nonalcoholic fatty liver disease. Results:The value of liver stiffness (LS) significantly rose in group F1 as compared with group F0 (8.74±1.32) kPa and (5.93±1.64) kPa respectively ( P<0.01). The value of LS had a significantly positive correlation with liver graft fibrosis stage ( r=0.73, P<0.01). The area under receiver operating characteristic curve (AUROC) was 0.93 for F1 stage fibrosis ( P<0.01). Significant differences existed in controlled attenuation parameter (CAP) among groups S0, S1 and S2 (173.30±38.36), (230.29±23.27) and (250.00±57.01) dB/m respectively ( F=12.41, P<0.01). CAP was correlated with liver graft steatosis stage ( r=0.64, P<0.01). And AUROC for S1/S2 stage steatosis in liver grafts was 0.89 ( P=0.002) and 0.83 ( P=0.007) respectively. Conclusions:With a high diagnostic accuracy, FibroScan quantifies fibrosis and steatosis in liver grafts from DBD and provides further imaging evidence for assessing liver grafts.