Objective To investigate the role of Gly82Ser polymorphism of receptor for advanced glycation end-products (RAGE) in the genesis and progression of colon cancer in Chinese population.Methods Using the method of PCR-restriction fragment length polymorphism (PCR-RFLP),the Gly82Ser genotype of RAGE were examined in 90 colon cancer patients and 78 control subjects age and sex matched.Analyses stratified by TNM and tumor differentiation were conducted to check the associations of the Gly82Ser gene polymorphisms in RAGE and development of colon cancer.Results The genotype distribution was in agreement with that predicted under Hardy-Weinberg equilibrium for both patients and controls (both P ＞ 0.05).With the GG genotype as reference,the odds ratio (OR) for heterozygous GG and carriers with S allele (GS and SS) were 2.037 (95％ CI:1.207-3.438) and 2.022 (95％ CI:1.275-3.208),respectively,which had a significantly higher risk of colon cancer.Moreover,the elevated colon cancer risk was especially evident in patients with TNM (Ⅲ + Ⅳ) and/or patients with poor differentiation by stratification analysis (OR,3.575,95％ CI:1.495-8.550 and OR,3.580,95％ CI:1.390-9.217,respectively).Conclusions The RAGE Gly82Ser polymorphism may confer not only an increased risk of colon cancer but also with invasion of colon cancer in the Chinese population.

OBJECTIVETo study the active compounds from the rhizomes of Musa basjoo.

METHODAntioxidant and alpha-glucosidase inhibitory activity of different extracts were tested. Using bioassay-guided fractionation, the chemical constituents in EtOAC extracts were isolated by column chromatography and identified by MS and NMR spectroscopy.

RESULTFive compounds were isolated and identified as 2',3, 4'-trihydroxyflavone (1), 3,3'-bis-hydroxyanigorufone (2), irenolone (3), 4-dihydroxy-9-(4'-hydroxyphenyl)-phenalenone (4) and 3,4-dihydroxybenzaldehyde (5). Compound 1(IC50 8.61 mg x L(-1)), 3 (IC50 19.55 mg x L(-1)) and 5 (IC50 1.1 mg x L(-1)) had antioxidant activity. Compound 2 (IC50 24.15 mg x L(-1)) and 4(IC50 2.81 mg x L(-1)) had alpha-glucosidase inhibitory activity. Compound 5 showed MIC of 0.078, 0.313, 0.039 microg/disc against SA, MRSA and ESBLs, respectively.

CONCLUSIONCompound 1-5 were isolated from this plant for the first time. Compound 5 was isolated from the genus Musa for the first time. All compound except 5 were first reported about activity.

Bacterial Proteins ; analysis ; antagonists & inhibitors ; Enzyme Inhibitors ; analysis ; isolation & purification ; pharmacology ; Glycoside Hydrolase Inhibitors ; Musa ; chemistry ; Plant Extracts ; analysis ; isolation & purification ; pharmacology ; Rhizome ; chemistry ; Staphylococcus aureus ; drug effects ; enzymology ; alpha-Glucosidases ; analysis

Objective To analyze the epidemiologic characteristics and epidemic intensity of measles in China between 2005 and 2013 , and to provide evidence for reasonable allocation of health resources and measles elimination in China . Methods Descriptive epidemiological methods were applied to measles surveillance data and demographic information during this study.Results A total of 597 594 cases, including 366 deaths, were reported from all over China , among whom 355 409 were males (59.47%) and 242 185 were females (40.53%).The average annual incidence was 5.07/100 000 and the average annual mortality was 0.0031/100 000.The majority of cases were children under 10 years old, accouning for 64.64%.Conclusion The incidence of measles in China is decreasing .Measles mainly occurrs in spring and there is significant difference between age and gender groups , and obvious regional differences are also found .

Objective To explore the neural damage induced by acute exposure to methamphetamine (METH).Methods The mice were administrated with METH,then the stereotyped behavior of mice was evaluated,and spatial recognition memory was analyzed by Y-maze test.In addition,nitric oxide synthase (NOS) activity was detected by kit,and the apoptotic proteins including Bax,Bcl-2,Caspase-3 were assayed by using Western blot.The DNA injury induced by METH was observed by using the comet assay.Moreover,mitochondrial membrane potential was detected to assess the toxic effects of METH on mitochondria by JC-1.With the Western blot assay,the phosphorylation of MAPK signaling pathways were also investigated.Results Acute METH exposure significantly increased the stereotyped behavior in mice,and spatial recognition ability of mice was obviously decreased.On the molecular level,total nitric oxide synthase (TNOS) and induced nitric oxide synthase (iNOS) were increased,and the apoptotic proteins,such as Bax and cleaved caspase-3 were markedly enhanced.With the comet assay,it showed that METH exposure resulted in DNA damage.In parallel,mitochondrial membrane was damaged which manifested as mitochondrial membrane potential decreased.With the western blot,It was further found that METH enhanced the activation of MAPKs.However,p38 MAPK signahng pathway was demonstrated to be the only one factor involved in METH-induced neural damage.Conclusion METH induced neural damage,and MAPK signaling pathways might be involved in this process,since inhibition of p38 MAPK signaling pathway significantly ameliorated METH-induced neural damage.

OBJECTIVETo study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase.

METHODEtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques.

RESULTNine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase.

CONCLUSIONAll compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.

Enzyme Inhibitors ; chemistry ; Glycoside Hydrolase Inhibitors ; Lythraceae ; chemistry

Curriculum integration and problem based learning (PBL) is the development trend of medical education all over the world,and it is an effective mode to train high-quality medical talents to meet the needs of the times and national medical and health services.However,PBL is difficult to popularize in an all-round way at present due to the large enrollment of clinical medicine major in medical colleges and universities in China.Aiming at integrating knowledge and focusing on ability,Nanjing Medical University implemented a problem-based two-way integrated case teaching after top-level design,careful trial and steady promotion and has achieved initial results and improved the clinical competency of medical students.

Health service is an important part of the integrated development of the Yangtze River Delta. Taking the cooperation practice between Shanghai Tenth People′s Hospital and Suzhou Yinshanhu Hospital as an example, this article introduced the multi-agent cooperation mode of the loose medical alliance including the government, urban hospitals and cross provincial grassroots medical institutions. Among them, the local government provided policy, fund guarantee and guidance, the urban hospital exported management ideas, medicine talents and technologies, and the primary hospital conducted dual training by inviting in and going out to achieve double growth. Through the high gap cooperation between tertiary hospital and primary hospital, Yinshanhu hospital had been comprehensively developed. The loose medical alliance with multi subject coordination and cross region could give full play to the advantages of the loose healthcare alliance mode, achieve multi-win, and have reference significance for promoting the regional integration of medical and health services in the Yangtze River Delta.

Organ transplantation is the most effective treatment for all categories of end-stage organ diseases. To resolve the shortage of donors in organ transplantation, widespread attention has been diverted to xenotransplantation. At present, clinicians mainly highlight the problems related to xenotransplantation rejection and viral infection. The physiology of xenotransplantation has been rarely studied. Kidney performs endocrine function by producing erythropoietin (EPO), renin and activating vitamin D. Although these pathways are usually well preserved in allogeneic transplantation, species-specific differences, especially those between pigs and non-human primates, may still affect the physiological function of transplant organs. In this article, the changes of EPO, renin-angiotensin-aldosterone system (RAAS) and active vitamin D₃ of pig and human after xenotransplantation were illustrated, aiming to provide reference for subclinical research of xenotransplantation.