Lobaplatin is the third generation platinum drug.Many studies on pharmacodynamics have confirmed that lobaplatin can block the cell cycle and induce cell apoptosis,and thus inhibits tumor cell proliferation effectively.Lobaplatin is not cross-resistant to cisplatin.Besides,it has good curative effect to various kinds of cancer patients with mild adverse reactions,and the patients are well tolerable to it.

Objective To evaluate the effect of splenectomy on hematological and immune system for children with major thalas‐semia .Methods This retrospective study was performed on 68 children with major thalassemia .The indexes of blood and immune system included white blood cell ,red blood cell ,hemoglobin ,platelet ,immunoglobulin IgG ,IgM and IgA and serum complement C3 , C4 ,which were recorded at pre‐splenectomy ,3 months and 3 -12 months post‐splenectomy .Results The mean age of children in this study was 8 .6 ± 3 .1 years .There were 28 children with α‐thalassemia ,35 with β‐thalassemia and 5 with αβ‐thalassemia .The white blood cell ,red blood cell and platelet count increased significantly both at 3 and 3-12 months post‐splenectomy as compared with pre‐splenectomy .The red blood cell and hemoglobin in children with β‐thalassemia or αβ‐thalassemia were significantly lower than children withα‐thalassemia both at pre and post‐splenectomy .However ,the platelet count were similar .The immunoglobulin IgA and IgM were significantly lower at post‐splenectomy than pre‐splenectomy .However ,the IgG ,C3 and C4 did not decrease sig‐nificantly .Conclusion Splenectomy could increase the hemoglobin level and improve the anemia in children with major thalassemia . However ,it might be harmful to the immune system .

Psychological behavior training curriculum in military medical college was recon-structed after investigating psychological behavior training class in 45 military colleges. The measures taken included:establishing scientific management model, optimizing the teaching object and content, reforming the teaching method and improving multiple assessment methods. Self rating symptom scale symptom checklist 90(SCL-90) was used to test the psychological condition of students before and af-ter the teaching experiment. Results showed that seven factors were of statistical significance for stu-dents. Factors of fear and paranoia were statistically insignificant.

Objective To investigate the distribution characteristics of motor endplates in musculuslevatorani of dif-ferent gestational ages. Methods 20~24 weeks female fetuses were categorized as Group A, and 24~28 weeks female fetuses as group B. Karnovsky-Roots iron cyanide copper staining ( hematoxylin) was used to study the aver-age weight, the morphology, distribution and changes in number of motor end plate. Results The motor endplates morphology and distribution of consistent oval of 20 ~28 weeks female fetuses showed a curve distribution in the musculuslevatorani fibers foreign 1/3 . The weight and numbers of motor endplates in Group A were less than those of group B. Conclusion 20 ~28 weeks female fetuses' musculuslevatorani are visible on the motor end plates, and no significant difference is found in morphology and distribution of the curve along the muscle fibers. The num-ber of motor endplates increases with the increase of anal muscle weight mentioned.

Aconite roots are a common clinical poisonous herb in Chinese herb. It has the effect of restoring yang and rescuing collapse, supplementing fire and assisting yang, eliminating cold and relieving pain. It is used in the treatment of yang collapse and yang deficiency syndromes. It is called principle medicine of all medicines. Monks-hood polysaccharide is one of effective components in Aconite roots. The study found that monkshood polysaccharide has effects of immune regulation, antitumor, myocardial preservation, lipid lowering, etc.

Objective:To establish an HPLC method with post-column derivation for the determination of aflatoxin B1 in edible vegetable oil. Methods:An advanced biotechnology-immunoaffinity column was used for the extraction of aflatoxin Bl from the samples, and an HPLC method with post-column derivation was applied to detect aflatoxin Bl in edible vegetable oil, and the results were com-pared with those of the national standard thin layer fluorescence method. Results:The linear range of aflatoxin Bl was 10. 2-51. 0 ng · ml-1(r=0. 9996), the average recovery was 87. 3%(RSD=0. 96%, n=6), and the detection limit was 1 μg · kg-1. Conclu-sion:The method is simple, rapid and sensitive, which can be used as a promoted conventional method for the detection of a large number of samples.

AIM:To investigate whether the cigarette smoke extract (CSE) causes senescence of C2C12 myo-blasts and the relationship between senescence and histone deacetylase 2(HDAC2).METHODS: Murine C2C12 cells were induced to differentiate into myoblasts.The HDAC2 activator and inhibitor were used to investigate the effects of CSE in the myoblasts on cell senescence and the expression of HDAC2.The expression of HDAC2 at mRNA and protein levels was determined by real-time PCR and Western blot, respectively, and the positive cell rate of β-galactosidase staining for cell senescence was also detected.RESULTS:The optimal concentration of CSE was 60 mL/L and the intervention time was 24 h.After the intervention of CSE, the positive cell rate ofβ-galactosidase staining was increased, accompanied with the reduction of HDAC2 expression at mRNA and protein levels.The expression of HDAC2 at mRNA and protein levels was increased by 4, 5, 6, 7-tetrabromobenzotriazole (TBB), accompanied with the reduction of positive cell rate ofβ-galacto-sidase staining.Furthermore, when HDAC2 expression at mRNA and protein levels was reduced by HDAC2 inhibitor valp-roic acid, the positive cell rate of β-galactosidase staining was increased.CONCLUSION: CSE promotes the senescence by reducing the expression of HDAC2 in C2C12 myoblasts.

Objective To investigate the effect of PCIA with low-dose fentanyl on plasma endothelin (ET) after craniotomy. Methods 47 cases of selected craniotomy were divided randomly into two groups :experimental group (26cases) and control group (21cases) . Patients in experimental group were treated with PCIA (fentanyl 15ug/kg +ondansetron 8 mg+100 mL NS) but patients in control group were not given PCIA. Then HR,MBP, VAS, ET and side-effects were observed and compared between two groups before treatment and 0, 2, 4, 8, 12, 24, 48 h after treatment.Results In experimental group, HR was lower at 2, 4, 8, 12, 24 and 48 h after treatment than control group. MBP was lower at 0 and 2h after treatment in experimental group than control group. Plasma levels of ET were lower at 8, 24 and 48h after treatment in experimental group than control group. There were significant differences in VAS scores at 2,4,8,12,24 and 48 h after treatment between two groups. There was no significant differences in side reactions including consciousness, respiratory depression, vomiting and sedation between two groups. The incidence of nausea was higher in experimental group than control group. Conclusion PCIA with low-dose fentanyl after craniotomy has good analgesic effect and few side reactions, can reduce the formation of plasma endothelin, and then alleviate brain damage.

To clarify the meaning, role and significance of the new idea of the modern basic theory of traditional chinese medicine, We clarified its origin and evolution starting from the basic concept of idea in this paper, re-vealed the basic connotation and relationship of ideas, scientific ideas, Chinese medicine ideas, and the new ideas of the modern basic theory of traditional Chinese medicine, and tried to give proper definitions; summarized three roles of scientific ideas. According to this basis, we demonstrated the new idea is the scientific ideas with core spirit run-ning through rational questioning, scientific testing, research and conclusion being accepted three-point line cen-tral interlocking; and have three roles changing the theory of traditional chinese medicine, guiding the innovative research on chinese medicine and elevating the scholars' thoughts. We proposed yin and yang to refer to the hypothesis of body's physiology and pathology and prevention and treatment effects, given an empirical analysis a feasible way of medicine and philosophy separation and yin and yang turning to medicine; displayed the guidance of new idea and to show the scientific connotation and scientific value of the theory of traditional Chinese medicine.

Aim To study the pharmacokinetics characteristics of galanthamine hydrohromide sustained release tablets and conventional tablets in healthy volunteers after a single and multiple oral doses. MethodsA single and multiple oral doses of galanthamine hydrohromide sustained release tablets and conventional tablets were given to 20 healthy male volunteers in a randomized cross-over study. We developed an LC-MS assay using naloxone as the internal standard to determine the plasma concentrations of galanthamine, calculate the pharmacokinetic parameters and evaluate the relative bioavailability and sustained release characteristics of galanthamine hydrohromide sustained release tablet. Results The pharmacokinetic parameters of the sustained release tablet and conventional tablet obtained from the single-dose study were as follows: the HVD_12 C_max(time span during which the plasma concentration is at least half of the C_max value)were (15.4?1.7) h and (5.4?2.5) h, the retard quotients (R△,the HVD_12 C_max ratio of sustained release tablets to conventional tablets) of sustained release tablet was 3.4?1.4, the T_max were (4.4?1.5) h and (1.3?1.2) h, the C_max were (27.5?2.9) ?g?L-1 and (53.7?12.7) ?g?L-1.Results showed significant sustained release characteristics of the sustained release tablet. The relative bioavailability of the sustained release tablet was (95.9?14.2) %。The pharmacokinetic parameters of the sustained release tablet and conventional tablet obtained from the multi-dose study were as follows: the T_max were (3.0?1.6) h and (0.9?0.3) h,the CSS_max were (58.8?9.4) ?g?L-1 and (52.0?6.9) ?g?L-1,the CSS_min were (16.2?4.0) ?g?L-1 and (22.5?5.0) ?g?L-1,the C_av were (39.0?3.9) ?g?L-1 and (37.1?5.0) ?g?L-1,the DF were 1.1 ?0.3 and 0.8?0.1, respectively. Results of two one-side t test showed that AUC_SS、CSS_max、C_av of two tablets were bioeqivalent. Conclusion Results showed that the sustained release tablet and the regular tablet were bioequivalent in absorbed extent, and the sustained release tablet exhibited a good retarding effect in release.