Purpose:To summarize the base of electroacupuncture analgesia theory and the development of pain-reliever at present.Methods:By consulting a great deal of literatures,the mechanism of electroacupuncture analgesia is summarized from nerve,body fluid to molecule.The merits,kinds,using methods and notices of electroacupuncture are briefly introduced.Present research of Electroacuncture analgesia and its prospects based on traditional Chinese medical theory and modern science and technology are also summed up.Conclusion:This paper provides the basic theory for studying and developing the new style pain-relievers.

Objective To explore the reasonable nursing interventions of advanced schistosomiasis patients. Methods The medical records of 52 advanced schistosomiasis patients hospitalized from 2008 to 2013 were collected,and the nursing interven-tions were summarized. Results The 52 cases of advanced schistosomiasis included 38 men and 14 women,with a mean age of 65.8 years(57-75 years). Totally 53.8%of the subjects were schistosome positive by IHA test,67.3%positive by ELISA,and 21.2%positive of HBsAg. There were 13 cases of ascites type,34 cases of megalosplenia type,and 5 cases of dwarfism type of ad-vanced schistosomiasis. Following the therapy together with nursing interventions,73.1%achieved clinical cure,23.1%achieved stable,and 3.8%achieved improvement. The major nursing interventions involved basic nursing,diet nursing,treatment nursing, physiological nursing and surgical nursing. Conclusion The scientific and reasonable nursing interventions can improve the ther-apeutic efficacy and prognosis in advanced schistosomiasis patients,as well as improve their quality of life.

Objective Accumulating evidence has demonstrated that the sub-anesthetic dose of ketamine exerts rapid and ro-bust antidepressant-like effects though its action mechanisms are not yet fully understood .The aim of this study was to investigate the role of gamma-aminobutyric acid ( GABA) in the antidepressant effects of ketamine . Methods Thirty-two male Wistar rats were e-qually randomized into four groups: saline, ketamine, GABA, and GABA+ketamine.All the animals were implanted with a guide cannula into the lateral ventricle and on the eighth day after operation subjected to a 15 min forced swimming test (FST) for the estab-lishment of a depression model .At 24 h after modeling , the rats of the saline and ketamine groups were treated intracerebroventricularly with 2μL isotonic saline solution, and those of the GABA and GABA +ketamine groups with 50μg (2μL) GABA, followed by intrap-eritoneal administration of 1 mL saline in the former two groups and 10 mg/kg (1 mL) ketamine in the latter two groups 10 min later.At 30 min after treatment , the open field test ( OFT) was carried out for crossing and rearing scores and a 6-min FST was performed to re-cord the immobility time in the last 5 minutes.The content of GABA in the prefrontal cortex of the rats was measured following behav -ioral tests. Results The immobility time was significantly decreased in the ketamine group ([107.5 ±21.2]sec) as compared with the saline, GABA, and GABA+ketamine groups ([167.2 ±22.1], [159.8 ±17.5], and [143.8 ±22.1]sec) (P<0.05), with no significant difference between the GABA and GABA +ketamine groups (P>0.05).The level of GABA in the prefrontal cortex was remarkably lower in the ketamine group ([12.4 ±3.4]ng/mg prot) than in the saline, GABA, and GABA+ketamine groups ([23.3 ± 6.3], [27.3 ±5.7], and [18.0 ±5.4]ng/mg prot) (P<0.05), but markedly higher in the GABA than in the GABA +ketamine group (P<0.05).OFT scores exhibited no significant difference in the lo-comotor activity among the four groups of rats ( P >0.05 ). Conclusion The antidepressant effects of ketamine are related to the decreased GABA level in the prefrontal cortex in rats receiving FST .

Ginseng root saponins ( GRS ) was extracted from native ginseng.The dosage used was calculated according to the content of saponins.Immunosuppression in mice was induced in 15 days after implar- ting Ehrlich ascites carcinoma (EAC) subcutaneously in armpit, and in 5-11 days after implanting EAC into peritoneal cavity. GRS administered orally 50mg/kg- for 8 days did not prevent the atrophy of the thymus in tumor-bearing mice but rather aggravated it. GRS administered orally 50mg/kg for 14 days partially restored the suppressed phagocytosis of peritoneal macrophages in tumor-bearing mice. GRS administered orally 50mg/kg for 6 days somewhat restored the suppression of hemolysin formation in tumor-bearing mice 5 days after implanting EAC, But GRS given in the same route and dosage for 9 days, showed no effect on the suppression of hemolysin formation. GRS administered orally 50mg/kg for 10 days partially restored the suppression of delayed hypersensitive reaction in tumor-bearing mice.

BACKGROUND: Transplantation of microencapsulated rabbit Schwann cells in the rat spinal cord can relieve inflammatory reaction, promote spinal cord regeneration, but the precise mechanisms remain unclear. OBJECTIVE:To observe basic fibroblast growth factor (bFGF)expression and movements recovery following transplantation of microencapsulated rabbit Schwann cells in rat spinal cord. METHODS: The sciatic nerves taken out from rabbits wore digested with mixed enzyme and were made into Schwann cells suspension. Then we used air-jet method to make Schwann cells microcapsule. Using the same method, empty microcapsule was made. Sprague Dawiey rats were randomly divided into cell group, empty microcapsule group and microcapsule group. Conducted by hemisection injury of spinal cord,the rats in cell group,empty microcapsule group and microcapsule group were implanted with gelatin sponge with 10μL Schwann cells suspension, gelatin sponge with 10 μL empty microcapsule and 10 μL microencapsulated Schwann cells. Normal group was left intact. After operation, we observed hindlimb movements recovery in rats with the Basso, Beattie, and Bresnahan (BBB) scale. Meanwhile,a set of sections were stained immunohistochemically for bFGF expression, another set of sections wore stained for hematoxylin-eosin and Nissal. RESULTS AND CONCLUSION: After spinal cord injury, rat right hindlimb affected paralysis immediately. At 7, 14 and 28 daysfollowing transplantation,motor function in rat hindlimb was significantly recovered, and the BBB scores were significantly higher in microencapsulated schwenn cells than in cell and empty microcapsule group (P < 0.05 or P < 0.01). bFGF positive products were mainly distributed in cytoplasm of the spinal neuron and nucleus of neuroglical cell. The numbers of bFGF positive glial cells mainly appeared surrounding the spinal cord injured site on days 1, 3, 7 and rose to its peak on day 3 and began to appear in neuronal calls on day 14. The number of bFGF positiv cells in microcapsule group was significantly superior to that in cell group and empty microcapsule group. From then on, the bFGF expreSsion was significantly decreased in each group. These indicated that transplantation of microencapsulated Schwann cells can inhibit the immunological rejection after xenotransplantation, suppress inflammatory reaction, improve the expression of bFGF, increase hindlimb movements recovery and spinal cord regeneration after spinal cord injury.

ObjectiveTo investigate the clinical efficacy of coiling-dragon warm needling atHuatuo jiaji points plus Chinese herbal fumigation in treating ankylosing spondylitis (AS).MethodEighty AS patients were randomly allocated to treatment and control groups, 40 cases each. The treatment group received coiling-dragon warm needling at Huatuo jiaji points plus Chinese herbal fumigation and the control group, oral administration of salicylazosulfapyridine (SASP). An investigation was made of the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), the patients’ overall assessment, the Bath Ankylosing Spondylitis Functional Index (BASFI), the total number of swollen joints, the Bath Ankylosing Spondylitis Measurement Index (BASMI) and laboratory inflammatory indices: erythrocyte sedimentation rate (ESR), platelet (PLT) and C reactive protein (CRP).ResultThere was a statistically significant difference in the marked efficacy rate between the two groups (P<0.05). Clinical indices: disease activity index and functional index and laboratory indices: ESR, PLT and CRP improved somewhat inboth groups of patients after treatment (P<0.05). After two courses of treatment, the therapeutic effect was better in the treatment group and there was a statistically significant difference compared with the control group (P<0.05).ConclusionCoiling-dragon warm needling at Huatuo jiaji points plus Chinese herbal fumigation can effectively relieve the clinical symptoms and reduce inflammatory reactions in the active stage in AS patients. It is an effective way to treat AS.

Objective Detecting the changes of the expression of vascular endothelial growth factor(VEGF) during rat liver regeneration so as to study the role of VEGF in the course of liver regeneration. Methods Three hundred adult SD rats were randomly divided into three groups:control group,sham operated group and test group.After taking out liver tissue and isolating hepatocytes at different phases after 2/3 rat hepatectomy,the change of VEGF expression was detected by both immunocytochemistry and Western blotting. Results The results of immunocytochemistry showed that VEGF positive cells were little in normal liver.The amounts of immunoreaction positive cells began to increase after 24 h of partial hepatectomy(P

Objective To investigate the effect of estrogen on regression of vascular calcification in rats induced by vitamin D3 plus nicotine.Methods Ninety-six female SD rats were divided randomly into control group (n=24) and calcification group (n=72).Vascular calcification of 72 rats was induced by vitamin D3 and nicotine (VDN).On the day 1,the VDN group rats were injected with vitamin D3(300 000 U/kg,i.m),and were intragastric administrated with nicotine (25 mg/kg),after 9 hours,another dosage of nicotine was given again.After 4 weeks,the VDN group rats were subdivided randomly into 4 groups:VDN group(n=16),Sham operation group (n=16),ovariotomy group (n=16),estrogen group(after ovariotomy,17β-estrogen was subcutaneously injected,50 μg· kg-1· d-1,n=16).Results After 4 weeks,the VDN group showed obvious vascular calcification,and calcium content of the vessel wall was significantly higher than that of control group (P＜0.01).Extensive calcification was witnessed on the aortic tunica media of the VDN group.After 12 and 8 weeks,the calcium content of the vessel wall in each subdivided groups was significantly lower than that at 4 weeks point(P＜0.01),and the lowest calcinm content was in estrogen group,meanwhile the reduction of previously accumulated arterial calcium precipitate in each group was different.Conclusions It is a reversible process that vascular calcification induced by vitamin D plus nicotine in rats.Estrogen can promote the regression of vascular calcification.

ObjectiveTo study effect of Herba Erigerontis on metabolites in cerebral ischemia rats with high-resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy(HR MAS 1H NMR). Methods18 male Wistar rats, weighing 150～200 g, were randomly divided into 3 groups such as normal (n=6), ischemia (n=6), Herba Erigerontis treatment (n=6). Rat ischemia model was established with middle cerebral artery occlusion (MCAO), reperfusing or dosed with Herba Erigerontis after 3 h ischemia. The metabolites, including N-acetylaspartate (NAA), creatine (Cre), choline (Cho), glutamate (Glu), and aspartate (Asp) etc., of cerebra and cerebella was observed using HR MAS 1H NMR. ResultsExcluding Cho, Glu, Asp, the concentrations of most metabolites of rat brain during ischemia were significantly lower than that of normal rats, and could be increased after drug treatment. ConclusionHerba Erigerontis shows positive effect on metabolites in cerebral ischemia rats.

This study was aimed to discover the knowledge of urination formula-syndrome in theTreatise on Exogenous Febrile Diseasebased on the Sunshine diagram of multi-layer complex concept network express. A total of 39 items about urination formula-syndrome in theTreatise on Exogenous Febrile Diseasewere collected, and then regulated into standard expression. The database was established and the multi-layer complex concept network express was constructed. The Sunshine diagram was drawn and the connotation rules on urination formula-syndrome in theTreatise on Exogenous Febrile Diseasewere summarized through mode development of the diagram. The results showed that the Sunshine diagram collected 44 objects (i.e., formulas) and 191 properties (i.e. syndromes), which expressed the urination formula-syndrome visually. It was concluded that the application of Sunshine diagram in the formula-syndrome knowledge based on multi-layer complex concept network express provided certain references on the inheritance and development of classics in traditional Chinese medicine (TCM).