Sixteen patients with congenital heart disease,4 males,12 females,aged 5-52 years,admitted to Department of Cardiology,Tianchang People's Hospital from October 2007 to November 2008 were retrospectively analyzed.All patients were diagnosed by echocardiography as 4 with congenital patent ductus arteriosus(PDA),6 atrial septal defect(ASD) and 6 ventricular septal defect(VSD).The diameter limitation of PDA,ASD and VSD are 5-17 mm,20-32 mm,and 4-10 mm,respectively.All patients received transcatheter-closure therapy by domestic-made occluders,and diameters of PDA,ASD and VSD were monitored using angiography and echocardiography.The treatment effect was evaluated by echocardiography 72 hours after procedure.The closure rate was 100%,without severe complications.No residual shunt was observed 72 hours after procedure.Four patients developed fever,including 2 acute respiratory infection and 2 pulmonary infection with balanced anesthesia;1 patient with ASD and 2 with VSDs developed arrhythmia.Results suggest that transcatheter closure of congenital heart defects(PDA,ASD and VSD) using domestic-made occluders is safe and effective according to strict indications.

Immune checkpoint is one of the most effective research targets for tumor immunotherapy. Immune checkpoint inhibitors, mainly programmed death receptor 1 and its ligand 1, have achieved good response rates in various tumor treatments, but some tumors still have low response rates. In recent years, bispecific antibodies have developed rapidly in the field of tumor research, because they can target multiple targets and play a combined role in tumor therapy, and can effectively inhibit tumor immune escape. This article reviews the research progress and clinical status of bispecific antibodies targeting immune checkpoints.

A total of 25 fast-growing rhizobium isolates from variety of legumiuous plants in Hainan Province, a tropical region of China, and 7 representative strains of genera Rhizobium, Sinorhizobium and Agrobacterium were characterized by numerical taxonomy. The results indicated that rhizobium isolates from Hainan were taxonomically heterogenous. At thesimilarity level of 70%, 13 strains from Hainan formed a distinct group which could use wider range of carbon and nitrogen sources, and were more resistent to antibiotics and chemicals than known rhizobium species. 2 strains could not fall into any group, and the others fell into different known species respectively. There were no correlations between hosts and the taxonomic positions of their symbionts. The rhizobium isolates from the same host genus or speceis could fall into different groups.

Objective:To develop bifunctional anti-human lingual cancer/anti-VEGF antibody.Methods:Antigen of lingual cancer was prepared from Tca 8113 cells and that of VEGF from endothelial cells of umbilical cord vein.The hybridoma cells of anti-human ligual cancer/anti-VEGF were prepared by the technique of secondary hybridoma.The antibody was identified by ELISA. Results:The specific conjugation rate between bifunctional antibody and lingual cancer tissue was 85.29%, and that between the antibody and vascular endothelial cells was 82.35%.Conclusion:The bifunctional antibody of anti-human lingual cancer/anti-VEGF has good targeting potential.

nephrectomy in Wistar rats were used to replicate the animal model of focal and segmental glomerular sclerosis.Then a group of 5/6 nephrectomized rats were given Benzaipril for 20 weeks , in order to evaluate the effects of Benazipril on degradation of extracellular matrix. The results showed that Benzipril not only reduced renal pathological changes, but also significantly decreased the type Ⅳ collagen and MMP 9 deposition and down regulated the mRNA expressions of MMP 9 and TIMP 1 in the remnant kidneys of the 5/6 nephrectomized rats. It suggested that Benzipril might ameliorate glomerulosclerosis through the mechanism of modulating extracellular matrix degradation in the remnant kidney tissue.

Purpose To summarize the CT features and clinical characteristics of atypical primary fallopian tube cancer (PCFT) and ovarian cystadenocarcinoma (OCA), in order to improved the diagnosis. Materials and Methods CT findings and clinical data of 12 patients with atypical PCFT (PCFT group) and 20 patients with OCA (OCA group) were retrospectively studied. All patients were confirmed by pathology. Serum CA125 level, clinical characteristics and CT features of two groups were compared. Results Serum CA125 level of PCFT group and OCA group were (486.13±23.89) U/ml and (1606.94±62.86) U/ml, respectively. There was statistic difference between the two groups (P<0.01). There was significant differences on vaginal bleeding and vaginal discharge between the two groups (P<0.01). There were 4 cases with solid mass, 8 cases with cystic solid mass in PCFT group. And in OCA group, 16 cases with cystic solid mass and 4 cases with cystic mass were revealed, which showed statistic differences between the two groups (P<0.05). Some other findings also showed statistic differences including the diameter of ovarian vein on the affected side, the diameter of the round ligament of uterus on the affected side, tumor volume, and CT values of the mass in venous phase and delay phase (P<0.05 or P<0.01). However, there were no statistic significance in the size of metastatic lymph nodes, the mean CT value of the mass on plain scan and in the arterial phase (P>0.05). Conclusion Clinical findings of serum CA125 level, vaginal bleeding, and vaginal discharge combine with imaging findings of diameter of the ovarian vein in the affected side, diameter of ipsilateral round ligament of uterus, tumor volume, CT values of the mass in venous and delay phase would be the key points for differential diagnosis of PCFT and OCA.

Objective:This study was aimed to investigate the influence of CDK7 siRNA on the sensitivity of endometrial carci-noma cell line HEC-1-A to cisplatin (DDP)-based chemotherapy. Methods:Different CDK7 siRNA fragments were synthesized based on the designs of the CDK gene sequence and were transfected into HEC-1-A. Real time reverse transcription polymerase chain reac-tion (RT-PCR) and Western blot analysis were employed to demonstrate the effects of transfection. The best CDK7 siRNA was chosen to specifically silence CDK7 expression in HEC-1-A.The sensitivity of the cells to DDP therapy before and after transfection was deter-mined by methyl thiazol tetrazolium (MTT) cytotoxicity assay, flow cytometry, and Hoechst/PI double-staining fluorescence microsco-py. Results:A total of four different CDK7 siRNA segments were designed and successfully transfected into HEC-1-A cells. The inter-ference effect in each group was confirmed by real time RT-PCR and Western blot assays. CDK7-423 was determined as the best per-forming CDK7 siRNA (over 70%) to transfect into HEC-1-A cells. MTT cytotoxicity test showed that IC50 of DDP decreased to a range from 45.122 μg/mL and 3.200 μg/mL after inhibition of CDK7 expression. DDP toxicity to the endometrial carcinoma cells sig-nificantly increased (P<0.05). Flow cytometry revealed that the average cell apoptosis rate significantly increased after the inhibition of CDK7 expression (11.66%to 37.57%, P<0.05). Similar results were observed using Hoechst/PI double-staining fluorescence microsco-py, and the number of apoptotic corpuscle demonstrated an apparent increase in the low CDK7-expressing group compared with the pa-rental cells. Conclusion:After the downregulation of CDK7 expression by CDK7 siRNA transfection, DDP chemotherapy sensitivity and apoptosis of endometrial carcinoma cells significantly increased. Further research is anticipated on the use of CDK7 as a new treat-ment target for endometrial carcinoma.

Objective To explore the clinical and laboratory characteristics of patients with dengue fever(DF)in Guangzhou from July to September in 2006 and to improve the awareness of dengue virus infection for physicians.Methods Clinical data of 277 patients with DF admitted to our hospital in Guangzhou were retrospectively analyzed.Dengue virus was isolated from patients' blood specimens by C6/36 cell culture and typed by reverse transcriptase-polymerase chain reaction(RT-PCR)assay and gene sequencing.Results Age of the 277 patients,148 males and 129 females,averaged 36 years with a standard deviation of 17 years,ranging from 9 months to 78 years.Their most common symptoms included fever(100.0%),skin rash(85.2%),headache(69.3%),myalgia(51.3%),bone soreness (39.0%)and nausea or vomiting(39.4%),and leucopenia and/hromboeytopenia occurred in 75.5% and 62.5% of them,respectively.Elevated alanine aminotransferase(ALT)was found in 59.6%,elevated aspartate aminotransferase(AST)in 81.6%,and hypopotassemia in 53.8% of them.Serum positivity of antibody against dengue virus IgM was 90.6%,which could be detected 3 to 10 days after onset of the illness,with a mean of 6.2 days with a standard deviation of 1.9 days.Cell-cultured virus from 20 patients were identified as typed by RT-PCR and RNA sequence analyses.All patients were clinically classified as typical DF and all of them completely recovered.Conclusions DF during this epidemic in Guangzhou was caused by dengue virus-1,with typical manifestations in all the patients but more damage in the liver.Some patients demonstrated hypopotassemia,but no dengue hemorrhagic fever or dengue shock syndrome was found.All the patients experienced a good prognosis with timely diagnosis and treatment.

Objective To compare the clinical value of injury severity score (ISS) and CRAMS (circulation,respiration,abdomen,motor,speech) score in the prognosis of patients with severe multiple trauma in emergency department.Methods A total of 93 severe multiple trauma patients with 69 male and 24 female and age ranged from 21 to78 years with mean age (43.4 ± 14) years admitted in the emergency department from March 2014 to December 2015 were enrolled into this study.All of them were evaluated by using ISS and CRAMS score respectively and their survival during hospitalization was observed.Then,the predictive capabilities of ISS and CRAMS score for the mortality of patients were assessed by using receiver operator characteristic (ROC) curves.Results The mean ISS was (25.59 ± 5.37) and mean CRAMS score was (6.75 ± 1.26) in 93 patients with severe multiple trauma.Compared with the survival group (n =75),the ISS in the death group (n =18) was significantly higher (t =7.13,P ＜0.01) and CRAMS score was significantly lower (t =-6.03,P ＜0.01).In the ROC curve analysis,the AUCROC of predicting mortality of multiple trauma patients by ISS score was 0.897 and CRAMS score was 0.891,respectively.There was no significant difference in accuracy of predicting mortality between the two scoring systems (Z =0.718,P ＞ 0.05).When the death was chosen as the prediction target,the optimal cut-off point for ISS was 27 and CRAMS score was 6,respectively.Conclusions CRAMS score in comparison with ISS score has the likewise predictive value for the prognosis of severe multiple trauma patients in emergency department.However,the CRAMS score is simple and easy in practice.The changes of CRAMS score demonstrate the severity of trauma timely and reliably,which is in favor of the diagnosis and treatment of hospitalized trauma patients in early stage.

Objective To investigate the diagnostic value of barium stury of small bowel obstruction due to various bowel diseases.Methods Barium meal study and enteroclysis (small bowel enema)were performed in 23 patients with incomplete small bowel obstruction due to small bowel diseases confirmed by operation.The imaging data were retrospectively analysed.Results Of the 23 patients,14 cases were malignancy,9 cases were benignant.Conclusion Both barium meal study and enteroclysis( small bowel enema) with air-barium double-contrast technique are of diagnostic value in incomplete small bowel obstruction due to small bowel diseases.