Objective:To establish the analysis method for the determination of Ni, Cu and Zn in aluminum food packaging mate-rials by inductively coupled plasma atomic emission spectrometry ( ICP-AES) . Methods: A small amount of EDTA was added to the test solution till the concentration at 0. 010 0 mol·L-1 and the pH value was adjusted to 5. 0. Strong acid cation exchange fibers were used for the assisted extraction of Al3+ with ultrasonic. Al3+ could not quickly form a complex anion with EDTA, so it was adsorbed by the strong acid cation exchange fibers. Cu2+, Ni2+ and Zn2+ could form complex anions with EDTA rapidly, so they could not be ad-sorbed by the strong acid cation exchange fibers and were left in the solution to be determined. Results:After the separation, the con-tents of Cu, Ni and Zn were not lost, and the content of residual aluminum was about 170 times of that of Cu, Ni and Zn, which had not interferece with the determination of Cu, Ni and Zn. The sample standard addition recovery was within the range of 98. 3%-104%with RSD of 0. 2%-2. 5%(n=5). The relative errors between the measured values and the certified values of standard substances were less than 5. 0%. Conclusion:The proposed method can be successfully applied in the separation and determination of Cu, Ni and Zn in aluminum cans, barbecue aluminum foil and standard substances.

Objective The aim of the study was to analyse the middle ear function of one hundred and twenty-five newborns using 226,678,1 000 Hz tympanometry. Methods Tympanometries with 226,678,1 000 Hz probe tones were obtained from 125 newborns (250 ears) by means of GSI Tympstar middle ear analyzer. The newborns with abnormal tympanograms were referred for temporale CT. Results One case of 226 Hz tympanograms was type "As" while others were all type "A",of which single-peaked tympanograms was 43.2%(108/250),W-shaped tympanograms was 56.8%(142/250). The abnormal pattern of 678 Hz tympanograms were 74 ears,and the abnormal pattern of 1 000 Hz tympanograms were 35 ears. 43 newborns (86 ears) have accepted temporale CT and results showed 30 middle ears with effusion. Conclusion 226 Hz probe tone tympanometry couldn't reflect the middle ear function of newborns. The sensitivity of 678 Hz probe tone tympanometry in the diagnosis of middle ear function of newborns was good,but specificity was worse. The sensitivity and specificity of 1 000 Hz probe tone tympanometry were the best.

Objective To study the effects of multi frequency tympanometry and acoustic stapedius reflexes on the diagnosis of the middle ear functions of infants.Methods Tympanometries with 226,678 and 1 000 Hz probe tones and 500 and 1 000 Hz acoustic stapedius reflexes were performed on infants at age 0~1 year.128 normal infants(256 ears) and 104 abnormal infants(168 ears) were tested with GSI Tympstar middle ear analyzer.Results The patterns of 226 Hz tympanograms were non-peaked,single-peaked and W-shaped.There were no significant differences between two groups of infants aged 0～6 months.The patterns of 678 Hz and 1 000 Hz tympanograms showed significant differences between two groups of infants aged 0～1 year.The outcomes of 500 Hz and 1 000 Hz acoustic stapedius reflexes showed no differences between the same age groups.There were significant differences between normal group and abnormal group.Conclusion Tympanometry with 678 Hz and 1 000 Hz probe tones can yield accurate diagnostic results in the assessment of the middle ear functions for infants younger than six months.The tympanometry with three frequency probe tones is sensitive to infants aged 6～12 months.The outcomes of acoustic stapedius reflexes at 500 and 1 000 Hz show no differences between the same age groups,but presences become more noticeable as a function of ages.

Objective To observe the clinical efficacy of Li Jiao Tong Fu (regulating the triple energizers and unblocking the Fu-organs) acupoint thread-embedding in treating functional constipation. Method Sixty eligible subjects were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by acupoint thread-embedding at Tianshu (ST25), Shuidao (ST28), Zusanli (ST36), Shangjuxu (ST37), and Danchangshu (BL25), once every 2 weeks, totally for 2 sessions. The control group was by sham thread-embedding at the same points with the same treatment duration. The Patient Assessment of Constipation-Symptom (PAC-SYM), Patient Assessment of Constipation-Quality of Life (PAC-QOL), Self-rating Anxiety Scale (SAS), and two factors from PAC-SYM, hard bower movement and bloating, were observed before and after the intervention, and the clinical efficacies were compared. Result The total effective rate was 83.3% in the treatment group versus 46.7%in the control group, and the total effective rate of the treatment group was significantly higher than that of the control group (P<0.05). The PAC-SYM, PAC-QOL, and bloating scores were significantly improved after the intervention in the two groups (P<0.05), and the improvements in the treatment group were more significant than that in the control group (P<0.05). The score of hard bower movement was markedly improved after the intervention in the treatment group (P<0.05), and the score in the treatment group was significantly lower than that in the control group (P<0.05). Conclusion Li Jiao Tong Fu acupoint thread-embedding is significantly effective in treating functional constipation, and it’s easy-to-operate, safe and reliable, and has a good compliance, thus worth promoting in clinic.

Objective: To examine correlations between MRI and cognitive changes associated with cerebral infarction. Methods: Using HDS-R and Senior Cognitive Scale, we assessed 101 patients with cerebral infarction during acute to rehabilitation phase. The results were compared with their MRI. Results: At 3 weeks after onset, cognitive impairment was found in 70.3% of the patients. At 6 months after onset, 10% recovered to normal, 35.6% improved, 22.8% worsened, and 42.5% met the criteria of dementia. During acute phase, infarction focus and leukoaraiosis (LA) were related to cognitive impairment ( P

Acupuncture analgesia, as a non-drug analgesic therapy, is widely used in the perioperative period clinically. In recent years, acupuncture analgesia in perioperative period is mainly used for preoperative sedation, intraoperative auxiliary anesthesia and postoperative analgesia, prevention of nausea and vomiting after surgery and perioperative complications, etc. This paper summarized its application law, influence factors, and the existing problems.

Recent studies show that resveratrol is characterized with its antioxidative and anti-inflammatory effects, and plays an important role in the prevention and treatment of colorectal cancer through modulating intracellular carbohydrate and ceramide metabolism, activating tumor suppressors such as activator of transcription 3 and peroxisome proliferators-activated receptor γ, down-regulating the expression of kirsten rat sarcoma viral oncogene and inhibiting the epithelial-to-mesenchymal transition process of cancer cells.In addition,resveratrol exhibits its broad application prospects in combination therapy.

Objective:To investigate the protective effects of paeoniflorin on myocardial ischemia/reperfusion ( MI/R) injury in rats. Methods:The rat model of MI/R injury was prepared by coronary artery ligation for 30 min followed by 2-hour reperfusion. Then the rats were randomly divided into 5 groups:sham group, model group, paeoniflorin group respectively at high, medium and low dose (20, 10 and 5 mg·kg-1, n=10). Paeoniflorin was respectively injected via tail vein 1 h before the operation and at the beginning of the reperfusion. The CK, LDH and SOD activities and MDA contents in serum of rats were examined, and the area of myocardial in-farction was also calculated after the reperfusion. Results:Paeoniflorin (20 and 10 mg·kg-1 ) could significantly reduce the area of myocardial infarction when compared with the model group (P<0. 05). Paeoniflorin could obviously inhibit the CK and LDH activities (P<0. 05 or P<0. 01) and enhance the SOD activity (P<0. 01) in serum of MI/R rats. The MDA content in paeoniflorin groups at high and medium dose was significantly lower than that in the model group (P<0. 05). Conclusion:Paeoniflorin pretreatment shows effectiveness against MI/R injury, which may be associated with the inhibition of lipid peroxidation.

Objective To study the clinical value of air-and hone-conduction auditory brainstem responses(ABRs) in children with congenital aural atresia.Methods Air-and bone-conduction clickevoked ABRs in 38 children with congenital external auditory canal atresia were compared to those in 34 normal-hearing children.Results ABR threshold were (65.0 ± 8.7) dBnHL for air conduction and (10.6 ±7.0) dBnHL for bone conduction in children with congenital external auditory canal atresia;and (25.6 ± 1.7) dBnHL and (9.7 ± 6.7) dBnHL respectively in normal hearing children.There was statistically difference between the two groups in air-conduction ABR threshold(t' =21.59,P ＜ 0.01).There was no significance between the two groups in bone-conduction ABR (P ＞ 0.05).While the air-bone ABR threshold gap was greater in children with external auditory canal atresia than in normal hearing children (t' =13.78,P ＜ 0.01),bone-conduction ABR wave latencies were not statistically different between the two groups(all P ＞ 0.05).Conclusion Bone conduction ABR is valuable in assessing fuuction of cochlea,auditory nerve and brainstem in individuals with congenital external auditory canal atresia.It has important clinical value in objective differential diagnosis of conductive deafness with combined application of air-and bone-conduction ABRs.

Objective To detect the expressions of Six1 ,TGF‐βand their common induced VEGF‐C in human laryngeal squa‐mous cell carcinoma ,and to explore their significance in the occurrence ,development ,metastasis and prognosis of laryngeal squa‐mous cell carcinoma .Methods The clinical data and preserved paraffin samples of 96 patients with laryngeal squamous cell carci‐noma confirmed by postoperative pathology were collected .The protein expression of Six1 ,TGF‐βand VEGF‐C was determined by adopting immunohistochemistry and Western blotting .Results The positive expression rates of Six1 ,TGF‐β and VEGF‐C in the laryngel squamous cell carcinoma tissue samples were 84 .4% (81/96) ,89 .6% (86/96)and 91 .7% (88/96)respectively .The positive expression rate of Six1 was significantly higher in the patients with poor differentiation and lymph node metastasis ;the positive ex‐pression rate of TGF‐βwas significantly higher in the patients with lymph node metastasis and had no relationship with the degree of differentiation ;the positive expression rate of VEGF‐C was significantly higher in the patients with poor differentiation and lymph node metastasis ;there was a positive correlation between expression of Six 1 and VEGF‐C in the laryngel squamous cell canc‐er tissue .Conclusion The high expression of Six1 ,TGF‐βand VEGF‐C may promote lymphatic metastasis of laryngeal squamous cell carcinoma .Six1 expression may be one of the important influencing factors of the expression change of VEGF‐C .The combined detection of Six1 ,TGF‐βand VEGF‐C has an important clinical significance for judging the metastasis and prognosis of LSCC .