Objective: To investigate the role of the expression of dephosphorylated RB protein for cell apoptosis in human breast cancer. Methods: In our works, human breast cell lines (MCF-7/S,the chemosensitive cell line and MCF-7/Adr, the chemoresistent cell line)were evaluated. The expressive levels of dephosphorylated RB protein were detected with immunocytochemistry. Apoptosis rates were determined by flow cytometry(FCM). Results: Before treatment of ADR, phosphorylated RB protein was positive in two cell lines, but dephosphorylated RB protein was negative; After treatment of different concentration ADR, when the concentration of ADR was increased, expression of dephosphorylated RB protein elevated accordingly in MCF-7/S, but no significant change in MCF-7/Adr. Apoptosis and cycle was detected by FCM assays shows ADR induced apoptosis of MCF-7/S more than MCF-7/Adr(P

OBJECTIVE:To explore the general rules and characteristics of ADR/ADE induced by Glycerol fructose injection and provide reference for clinical ration drug use. METHODS:284 cases of ADR/ADE induced by Glycerol fructose injection from Jan. 2004 to Oct. 2014 in Drug ADR Monitoring Center of Henan Province were statistically analyzed and comparatively analyzed with the drug instructions in 11 domestic drug manufacturing enterprises. RESULTS:ADR/ADE induced by Glycerol fructose injec-tion was related to gender,mainly adult male,and ADR/ADE might occurred within 30 min;main clinical manifestations were skin and its appendages damages,medication site damage and systemic damage. There were larger differences among the notes de-scription in the package inserts from 11 drug manufact urers. CONCLUSIONS:The clinic should strengthen the monitoring of ADR/ADE induced by Glycerol fructose injection,try to avoid or reduce the incidence of ADR,improve the drug instructions and ensure medication safety.

Objective To evaluate the value of the combination detection of serum amylase (AMY) and lipase(LPS) in the diag‐nose of acute pancreatitis .Methods Serum AMY and LPS were detected in 51 patients with acute pancreatitis ,418 patients with a‐cute abdomen disease and 78 healthy control subjects .Results The sensitivity of serum AMY and LPS increase in 51 patients with acute pancreatitis was 86 .3 % and 92 .2 % respectively .The specificity of serum AMY and LPS increase ,and both AMY and LAS increase in 418 non‐pancreatitis patients with acute abdomen disease was 90 .4% ,91 .7% and 100% respectively .Conclusion The combination detection of serum AMY and LPS can increase the sensitivity and specificity for diagnosing acute pancreatitis and has an important significance for rapid diagnosis of acute pancreatitis .

Objective To evaluate the therapeutic and adverse effects of GMEP regimen in the treatment of refractory or recurrent non-Hodgkin′s lymphoma(NHL). Methods A total of 30 refractory or recurrent NHL patients received a course of GMEP regimen: Gemcitabine (GEM) 1 000mg/m~2, IV, d1, 8; Etoposide (VP16) 60mg/m~2, IV, d1-5; Mitoxatrone (MIT) 6-8mg/m~2, IV, d1; Prednisone(PDN)60mg/m~2, PO, d1-5. The treatment was repeated every 3-4 weeks for 3 courses at least. Results All the 30 patients received a complete course of chemotherapy. Among them 28 (93.3%) were followed-up. The median survival time was 11.3 months. The 1-year and 2-year survival rates were 43.3% and 30.8%, respectively. The total remission rate was 80% (24/30), with CR 20% (6/30) and PR 60% (18/30), and type B symptoms were alleviated in 6 of 10 patients. The main adverse effects were myelotoxicity, nausea and vomiting. Conclusions GMEP regimen can be safely applied to the patients with refractory or recurrent non-Hodgkin′s lymphoma and the short-term response is comparable to the results of other treatment modalities.

Objective To explore the clinical value of clara cell protein 16 (Cc 16) in the early diagnosis of ARDS in critically ill patients.Methods A total of 55 critically ill patients admitted between March 2013 and December 2013 in the Intensive Care Unit were enrolled for study.The inclusion criteria were as follows:sepsis,pneumonia,multiple injuries,patients after emergency or elective operation and non-cardiogenic diseases,whereas the exclusion criteria were cardiogenic pulmonary edema,age ≤ 18 years or≥80 years and disease course prolonged over one week.The level of serum Cc16 was detected with enzyme linked immunosorbent assay (ELISA).In addition,data of other biochemical examinations,the Acute Physiology and Chronic Health Evaluation (APACHE]Ⅱ) score and the relevant medical data were documented.The patients were divided into ARDS groups and non-ARDS groups based on clinical data met Berlin definition.Results The sensitivity and specificity of serum Cc16 for diagnosis of ARDS were 92％ and 80％,respectively with the area under the curve being 0.92,which were better than those of APACHE Ⅱ score,D-dimer,C-reactive protein,N-terminal pro-brain natriuretic peptide and serum albumin detected by the means of receiver operating characteristic curve,and cut off value was 20.62 ng/L.The bivariate analysis showed there was negative correlation between Cc16 and oxagenation index in ARDS patients and the Pearson correlation coefficient of serum Cc16 with oxygenation index was r =-0.342 (P =0.04).The results of one-way analysis of variance showed difference in level of Cc16 between subgroups (F =15.76,P =0.005 17).The level of Ccl6 in severe ARDS group was (64.18 ± 12.95) ng/L which was higher than that in mild ARDS group (35.87 ± 11.28) ng/L (P =0.001 14),and in moderate ARDS group (38.66 ± 20.14) ng/L (P =0.004 9),and in non-ARDS group (16.72 ± 8.74) ng/L (P =0.000 32).There was no statistically significant difference in Cc16 level between mild ARDS group and moderate ARDS group (P =0.682).The level of serum Ccl6 did not correlate with type or days of respiratory ventilation support,28-day survival rate or 120-hour survival rate and days of ICU stay and hospital stay.Conclusions The diagnostic value of serum Cc16 is very high in determining the presence and severity of ARDS in addition to the Berlin criteria in critically ill patients accurately assessing degree of lung injury.

Objective To investigate the efficacy of intravenous antioxidants therapy in treating moderately severe acute pancreatitis (MSAP) and severe acute pancreatitis (SAP).Methods Pubmed,Embase,Cochrane library and CNKI databases for all randomized control trials published before March 18st,2016 manually was searched by computer.Data on AP associated mortality and length of stay (LOS) were collected.The quality of the trials included was assessed by the Cochrane systematic review method.Results Fifteen trials with data of 620 patients were eligible for final inclusion.Among the 15 trials,detailed randomization for grouping was clearly described in 5 studies and obvious bias was observed in 1 study.Three studies had obvious biases considering whether outcome assessment was blinded,outcome data was incomplete and outcome report was selective.No other apparent bias was found.Statistical analysis showed that compared with control group,intravenous antioxidants administration did not significantly reduce mortality (12.1 vs 9.7,RD=-0.02,95％ CI-0.08～-0.03,P=0.44;RR=0.83.95％ CI0.51～ 1.34,P=0.44),but could shorten LOS (MD=-2.02;95％ CI-4.00～-0.05;P=0.04).Conclusions Intravenous antioxidants could greatly shorten LOS of patients with MSAP and SAP.

Objective To investigate the effect of rapid repeated cold air inhalation stimulation on cold-sensitive chan-nel transient receptor potential ankyrin 1(TRPA1) associated inflammatory cytokines in living lungs .Methods A total of 20 male Wistar rats were randomly and evenly divided into 4 groups:cold air inhalation group , warm air inhalation group , TRPA1 channel agonist inhalation group and normal group , respectively . Tracheal intubation was carried out after anesthesia in rats of cold air inhalation group , and the tubes were linked to the air temperature controlled device with the temperature controlled at ( 2 ±1 )℃.The rats of warm air inhalation group were treated in the same way as cold air inhalation group except for the temperature at (36 ±1)℃.The rats of both groups were treated for 3 times, 1 hour each time.There were 12 hour intervals between treatments .The rats of TRPA1 channel agonist inhalation group were treated with atomizing inhalation of 60 mmol/L acrolein at room temperature (24 ±2)℃for 3 times, 1 hour each time.There were 12 hour intervals between two treatments .The rats were sacrificed after the last treatment .The tracheas and left lung tissues of all rats were taken and total RNA was extracted .The mRNA expressions of TRPA1, interleukin 1 beta ( IL-1β), interleukin 5(IL-5),and neutrophil chemoattractant chemokine (CXCL-1/KC)in the rat lungs were detected by real-time quantitative PCR .Results TRPA1 was expressed in rat lung tissues .In the short term cold air inhalation group , the expressions of IL-1β,IL-5 and CXCL-1/KC mRNA in the rat lungs were higher than those of the warm air inhalation or the normal groups .Similar results were also found in the specific TRPA 1 channel positive agonist ( acrolein ) inhalation group . Conclusion In living animals, the TRPA1 channels of the lungs can be activated by short-term cold air(<17℃),which results in the increasing expression of some inflammatory cytokines in lungs .

Objective To analyze the correlation of vertebral osteophyte and lumbar disc degeneration in the elderly people and explore the possible mechanism of osteophyte formation. Methods X-ray and MRI data of 120 elderly people with backache or leg pain were retrospectively analyzed. Osteophyte was classified into four grades by X-ray according to the method proposed by Nathan. Discs with osteophyte were defined as occurring when osteophyte of grade II or greater were present. Lumbar disc degeneration was classified into five grades by MRI according to the method proposed by Pfirrmann. The obtained parameters were statistically treated and analyzed. Results Osteophyte and age were positively correlated at every lumbar disc level (P < 0.05). The proportion of osteophyte in L3/4 (76.7%) and L4/5 (70.08%) were more severe than that in L1/2 (31.7%) and L2/3 (46.7%). Osteophyte and lumbar disc degeneration were positively correlated at every lumbar disc level (P < 0.01). Conclusion Osteophyte becomes more severe with the increasing of lumbar disc degeneration. The vertebral stress after lumbar disc degeneration may be the main cause of osteophyte.

Acute respiratory distress syndrome (ARDS) is a common diseases in critical ill patients, which is an acute-onset hypoxic condition with radiographic bilateral lung infiltration. And it can be divided into two phases in pathophysiology: an acute exudative phase combining diffuse alveolar damage and lung edema followed by a later fibro proliferative phase. The early prediction and risk assessment of patients with ARDS is still very poor in the clinical. In recent years, researchers gradually focus their research on exploration of biomarkers of ARDS. The article mainly aims to sum up the development of biomarkers exploration.

Objective To establish a distinct linked emergency rescue network system in Shenzhen. Methods The current status of emergency network system in Shenzhen was studied, and some related publications in the recent years were reviewed. Results The emergency network system in Shenzhen is characterized by the following aspects: there is an effective emergency dispatch system with high authorities; the network covers every corner of Shenzhen; series of laws have been issued to ensure the proper operation of network. Conclusion The medical rescue system has developed in depth. The combination of community-based, pre-hospital and hospital rescue is effective in the whole process of emergency rescue, and shortens the rescue distance. The community-based rescue is the basic part of the system that can use the medical resources efficiently.