1.Early clinical analysis of infants with cerebral palsy
Hanlin PENG ; Xiaobao SUN ; Wenwen HU
Chinese Journal of Primary Medicine and Pharmacy 2008;15(6):977-978
Objective To explore early clinical diagnosis of infant with cerebral palsy(CP). Methods Early diagnosis of cerebral palsy information abroad was referenced. DDST scale screening, newborn NBAS inspection stan-dards, VOJTA postural reflex and the nervous system reflective were used to evaluate the cerebral palsy. Results 90 cases CP of which 63 eases spastie. Xu mobile hand-foot-15 cases, 12 cases of mixed type. Conclusion Standardized,quantitative indicators,only workable and easy to use.
2.A Triple Level Registration Strategy of Medical Image Based on Mutual Information
Wenwen YI ; Faliang CHANG ; Yanlei HU
Space Medicine & Medical Engineering 2006;0(06):-
Objective To further solve the mutual restricted problems among precision, speed and disregistrated rate of registration algorithm based on mutual information (MI). Methods A new strategy on the search of registration parameters, a triple level registration strategy, was proposed. It searched the translation parameters and the rotation parameters respectively. Based on analyzing the advantage and limitation of MI-based registration, Euclid closeness of Fuzzy theory was introduced into the process of the triple level registration. Results The computer simulation results demonstrated that the proposed registration method, on the condition of retaining the same precision, improved both the speed (step numbers and registration time) and the disregistrated rate. Conclusion The proposed triple level registration strategy is a registration strategy to be able to harmonize precision, speed and disregistrated rate effectively.
3.Relationship between photoallergens and melasma
Zhiyu LIU ; Wenwen FU ; Yue HU ; Yu XU
Chinese Journal of Dermatology 2011;44(7):511-512
Objective To study the relationship between common photoallergens and melasma.Methods Twenty allergens were selected according to the National Diagnostic Criteria for Photosensitive Dermatitis induced by cosmetics,National Diagnostic Criteria for Contact Dermatitis induced by cosmetics and National Diagnostic Criteria for Occupational Skin Diseases.Based on the standard procedures for photopatch test recommended by the British Photodermatology Group (BPG),photopatch test was performed on 50 patients with melasma and 30 normal controls.Results The positivity rate of photopatch test was 48.0%(24/50)in patients suffering from melasma,significantly higher than in the controls[23.33%(7/30),P<0.05].Conclusion Patients with melasma appear more likely to have positive photopatch test than normal controls.
4.Study on technique of phase detection in magnetic induction tomography
Xiaoyan HU ; Mingxin QIN ; Mingke JIAO ; Wenwen LIANG ; Hua ZHANG
Chinese Medical Equipment Journal 1989;0(04):-
In the magnetic induction tomography(MIT) system,the electrical conductivity of biological tissue is direct proportion to the phase difference between the excitation signal and the detection signal.To obtain the image of the contribution of tissue's electrical conductivity,the system must have the function of phase detection with high accuracy.The paper focuses on the means of digital phase detection,including FFT method,the correlation method and the classic method,which are ultimately compared with analogue phase detection method.The experimental results show that FFT method and the correlation method,with low error level and high linearity,can better detect the phase difference with the level of 0.1?.The digital phase difference detection provides a kind of effective method for MIT system.
5.DDA and BCG polysaccharide nucleic acid improved the immunogenicity and protective efficacy of tuberculosis subunit vaccine against Mycobacterium tuberculosis infection in mice
Zejiao DA ; Lina HU ; Bingxiang WANG ; Wenwen JIANG ; Linfeng FU ; Hongjuan YU ; Yu LUO ; Bingdong ZHU
Chinese Journal of Microbiology and Immunology 2010;30(6):555-559
Objective To investigate the adjuvant effect of dimo-thylidioctyl ammonium bromide (DDA) and/or DDA-BCG polysaccharide nucleic acid( BCG-PSN), which was combined with a Mycobacterium tuberculosis fusion protein AMM ( Ag 8 5 B - MPT64190-198 - Mtb8.4 ) to boost BCG primed immunization. Methods DDA with or without BCG PSN was mixed with the fusion protein AMM to construct the boosting vaccine. Mice were immunized with BCG and then boosted twice with AMM formulated with the adjuvant DDA with or without BCG-PSN. PBS or BCG vaccination without boosting was used as control. The humoral and cell-mediated immune responses were analyzed by ELISA and ELISPOT. Moreover, the protective efficacy of BCG prime-AMM subunit vaccine boosting against Mycobacterium tuberculosis infection was analyzed. Results With in vitro stimulation of Ag85B and PPD( purified protein derivative) antigen, the number of IFN-γ secreting cells from the mice boosted twice by AMM/DDA/BCG-PSN and AMM/DDA were higher than BCG and PBS group (P <0.05). The CFU in lungs of mice boosted with AMM/DDA/BCG-PSN was less than that of PBS group(P <0.05), while the CFU of AMM/DDA-boosted mice was less than that of BCG and PBS group(P < 0.05).However, fewer lesions were seen in lungs of mice immunized with BCG alone or BCG-prime-AMM/DDA/BCG-PSN boosting than the other groups. Conclusion DDA is an idea adjuvant for tuberculosis subunit vaccine;BCG-PSN might play a role in alleviating the immunity-mediated pathology.
6.Influence of Celecoxib on Radiosensization of Irradiation ̄resistant Cell Line CNE ̄2R ofNasopharyngeal Carcinoma
Wenwen LI ; Wei SUN ; Min LUO ; Yu HUANG ; Linli ZHANG ; Guoqing HU
Herald of Medicine 2015;(11):1439-1444
Objective To investigate the effect of COX ̄2 inhibitor celecoxib on radiosensitity of irradiation ̄resistant cell line CNE ̄2R of nasopharyngeal carcinoma and the potential mechanism. Methods Via exposing to a series of X ̄ray (2, 4, 6, 8 Gy, 3 times for each dose), radio ̄resistant cell subline CNE ̄2R was established from human nasopharyngeal carcinoma cell CNE ̄2.Radiosensitivity was detected by clone formation assay.CNE ̄2R and CNE ̄2 cell lines were exposed to 25, 50, 75 μmol.L-1 celecoxib, respectively.Western blotting was used to detect the protein expression of COX ̄2.Clone formation assay was performed to measure the survival fraction of CNE ̄2 and CNE ̄2R after radiotherapy alone or radiotherapy combined with 30 μmol.L-1 celecoxib treatment.Flow cytometry was used to measure influence of radiotherapy alone or radiotherapy combined with 30 μmol.L ̄1celecoxib treatment on cell apoptosis.Number of residual γ ̄H2AX foci was observed by immunofluorescence assay. Results The colony forming assay demonstrated that the values of SF2, D0 , Dq , and N of CNE ̄2R cell subline [(0.81±0.05), (2.15±0.07) Gy, (2.94±0.08) Gy, (3.91±0.07), respectively] was significant higher than those of CNE ̄2 cell line [(0.61±0.08), (1.47±0.06) Gy, (1. 68 ± 0. 10) Gy, (3. 13 ± 0. 05), respectively]. The expression of COX ̄2 protein was significantly downregulated with increasing celecoxib concentration.Surviving fraction was decreased in both CNE ̄2 and CNE ̄2R cell lines after irradiation.After radiotherapy combined with celecoxib, apoptosis rates of CNE ̄2 and CNE ̄2R cell lines [(13.10±0.63)%, (5.30±0.75)%] were higher than those of the corresponding control groups [(4.90±0.71)%, (1.82±0.82)%].Celecoxib increased radiosensitivity in nasopharyngeal carcinoma CNE ̄2R and CNE ̄2 cell lines.The number of residual γ ̄H2AX foci after irradiation was increased by celecoxib pretreatment.The difference was statistically significant (P<0.05). Conclusion Celecoxib can enhance radiosensitivity of radio ̄resistant cell subline CNE ̄2R of human nasopharyngeal carcinoma in vitro.
7.Effect of Lycium barbarum polysaccharide on atherosclerosis in mice by up-regulating adiponectin expression
Lei HU ; Ling DONG ; Lei QU ; Libin WANG ; Wenwen WU ; Haifang WANG
Drug Evaluation Research 2017;40(3):292-299
Objective To explore the effect of Lycium barbarum polysaccharide (LBP) on adiponectin (APN) expression and the mechanism of lowering blood-lipid and anti-inflammation in atherosclerotic (AS) mice.Methods C57BL/6J mice with normal feed were chosen as control group.Thirty-two ApoE-/-mice with high cholesterol diet were successfully established as AS models,and then the mice were randomly divided into model group and three LBP groups,which were feed with high,medium,and low dose of LBP.After feeding for four weeks,aortic blood and tissues were collected.Blood-lipid,inflammatory factors,endothelin-1 (ET-1),APN,AdipoR1,and AMPK pathway related protein expression were detected.Differentiated 3T3-L1 cells were divided into control group,LBP group,and LBP + BML-275 group.Triglyceride (TG),inflammatory factors,APN,AdipoR1,and AMPK pathway related protein expression was investigated.Results In mice,compared with the control group,typical AS pathomorphologic changes were found in aorta and the levels of TG,total cholesterol (TC),nitric oxide (NO),ET-1,interleukin-6 (IL-6),and tumor necrosis factor (TNF-α) in the model group were significantly increased,while the protein expression of HDL-C,APN,AdipoR1,PPARα,AMPKα,and p-AMPK-α and Acyl-CoA oxidase (ACO) mRNA expression was reduced.Compared with the model group,AS pathomorphologic state was obviously improved in aorta and the amount of TG,TC,NO,ET-1,IL-6,and TNF-α in LBP groups were markedly decreased,while the protein expression of HDL-C,APN,AdipoR1,PPARα,AMPKα,p-AMPKα,and ACO mRNA expression was up-regulated.These changes were all in a dose-dependent manner.In differentiated 3T3-L1 fat cells,compared with control group,LBP enhanced the expression of APN,AdipoR1,PPARα,AMPKα,p-AMPKα,and ACO,but decreased the amount of TG,IL-6,and TNF-α.Compared with LBP group,the levels of TG,IL-6 and TNF-α was notably increased in BML-275 group.Conclusion LBP up-regulates the expression of APN and AdipoR1,activates APN/AMPK pathway,plays a role in lowering blood-lipid and anti-inflammation,and thus relieves AS in mice.
8.Myeliod sarcoma in cardio-phrenic angle after allogeneic peripheral blood stem cell transplantation
Yi HE ; Xudong LI ; Dongning WANG ; Yuan HU ; Wenwen WANG ; Jiajun LIU ; Dongjun LIN ; Renwei HUANG
Chinese Journal of Tissue Engineering Research 2013;(27):5041-5047
BACKGROUND: Al ogeneic hematopoietic stem cel transplantation is the main method to cure leukemia, but the patients receiving al ogeneic hematopoietic stem cel transplantation stil have to face the risk of recurrence. Myeloid sarcoma is a rare extramedul ary relapse mode with worse clinical outcomes, so it is necessary to understand the characteristics of myeloid sarcoma and relative treatment methods. OBJECTIVE: To analyze the clinical characteristics and treatment methods of myeliod sarcomas in cardio-phrenic angle after al ogeneic peripheral blood stem cel transplantation. METHODS: One case was diagnosed as single myeloid sacoma in the cardio-phrenic angle after al ogeneic peripheral blood stem cel transplantation. The patient underwent surgical resection of the mass, chemotherapy and radiotherapy. The clinical effect, complications and survival situation were observed. RESULTS AND CONCLUSION: The patient suffered from bacteremia, fungal pneumonia and even life-threatening sepsis shock during two courses chemotherapies. Then, the patient received radiotherapy for mediastinum and the myeloid sacoma never relapsed. However, the patient suffered central nervous system leukemia after free of the disease for 25 months. Myeliod sarcoma after al ogeneic hematopoietic stem cel transplantation is rare and its manifestation is changeful. The diagnosis mainly depends on histopathology and immunohistochemistry. Surgery, chemotherapy, radiotherapy, second transplantation and molecular targeted drugs are the choices of treatment strategy. However, the optimal treatment strategy for individual patients remains to be determined.
9.Observe the origin of antrochoanal polyp and the comparison of the curative effect of antrochoanal polyp.
Wenwen ZHENG ; Guoqin HU ; Bin LIU ; Shimin LIU ; Qin WANG ; Xiaofei SUN ; Hujun ZHOU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2016;30(3):209-212
OBJECTIVE:
To explore the curative effect and origin of antrochoanal polyp (ACP) with various approaches.
METHOD:
Fifty-seven patients with ACP were included in the study. All the ACP patients were examined by preoperative endoscopy and computer tomographic (CT) scans. The patients were treated by various endoscopic approaches including endoscopic middle meatus antrostomy, inferior meatus antrostomy combined with endoscopic middle meatus antrostomy or endoscopic medial maxillectomy combined with endoscopic middle meatus antrostomy respectively. The relationship between polyp location in middle meatus and lesions in the antrum was explored during the surgery. Pathological examination was carried out and patients were regularly followed up after operation.
RESULT:
Fifty-seven ACP develops from antral cyst. In 22 cases of endoscopic middle meatus antrostomy, two patients relapsed. In 17 cases of inferior meatus antrostomy combined with endoscopic middle meatus antrostomy, one patients relapsed. In 18 cases of endoscopic medial maxillectomy combined with endoscopic middle meatus antrostomy, no one relapsed.
CONCLUSION
Our data indicated that the ACP mainly originates in antral cyst, and capsule wall herniates to middle meatus through the antral ostium. ACP are common in unilateral, rare in both sides. The endoscopic approaches of middle meatus antrostomy and inferior meatus antrostomy combined with endoscopic middle meatus antrostomy might guarantee good prognosis. If the cyst is on the anterior wall of maxillary sinus, the approach of endoscopic medial maxillectomy can obtain a better vision and completely remove the lesions.
Cysts
;
surgery
;
Endoscopy
;
Humans
;
Maxillary Sinus
;
surgery
;
Nasal Polyps
;
surgery
;
Nasal Surgical Procedures
;
methods
;
Tomography, X-Ray Computed
10.Content Determination of Astragaloside in Danggui Buxue Formula Granule by HPLC
Guangsheng LI ; Fang QIN ; Jiling YANG ; Wenwen HU ; Junli ZHANG ; Weixin ZHANG
China Pharmacy 2015;26(36):5131-5133
OBJECTIVE:To establish a method for content determination of astragaloside in Danggui buxue formula granule, and investigate the influence of different conditions of dissolution on the content. METHODS:HPLC was performed on the column of Aglient C18 with mobile phase of acetonitrile-water(28:72,V/V)at flow rate of 1.0 ml/min,column temperature was 25 ℃,and the injection volume was 10 μl. RESUITS:The linear range of astragaloside was 2.91-17.46 μg;RSDs of precision,stability were lower than 2%,and reproducibility tests was 3.63%,recovery was 95.76%-98.20%(RSD=1.03%,n=6). The optimized use method was mixing the two granules with 80 ℃ hot water and keeping heat preservation for 5 min successively before taken. CON-CLUSIONS:The method is simple and reproducible,and can be used for the content determination of astragaloside in Danggui bux-ue formula granule.