Recently,studies have demonstrated that several signaling pathways including Wnt,Notch,and Hedgehog which are involved in the regulation of the stem cells are abnormally activated in lung cancer.They are closely associated with some properties of the lung cancer stem cells,such as high tumorigenic,high metastasis,drug resistance and so on.In addition,several studies have shown that the population of the lung cancer stem cells,which are resistant to radiotherapy and chemotherapy significantly,highly express drug resistance proteins.Therefore,how to target lung cancer stem cells and ultimately cure the disease is becoming a hotspot in the cancer targeted therapy.

By using three-dimensional finite element analysis method, the necessity of tail joints in the establishment of finite model specifically for the widely used tail-suspended rat experiments in weightlessness simulation was explored. A weightlessness tail-suspended three-dimensional finite element rat model was established using CT scan and Abaqus software, and the computation and analysis were conducted using the same model. The stress distribution and displacement in tail, lumbar spine, pelvis and femur of a tail-suspended rat both with and without cartilage were simulated and calculated.The results showed that stress distribution and displacement of tail-suspended rat were quite different between rats with cartilage and without cartilage, which affected the calculation precision of the model. Accordingly the consideration of articular cartilage in establishing the tail-suspended three-dimension finite element rat model is quite necessary,In addition, the asymmetry of pelvis stress distribution of tail-suspended rat suggests that the degree of balance of tail-suspended rat will affect the stress distribution in rats.

Objective To study the curative effect of Bairui capsule combined with azithromycin in treatment of children with mycoplasmal pneumonia and its effect on serum high sensitive C-reactive protein, eosinophil, soluble interleukin-2.Methods 86 patients with mycoplasma pneumonia were enrolled from August 2015 to July 2016 in our hospital.According to the order of admission, the patients were divided into observation group and control group.The control group was treated with azithromycin , and the observation group was treated with Bairui capsule and azithromycin.Clinical efficacy,symptoms and signs of disappearance of time, CRP, EC, SIL-2R and adverse reaction rate were compared between the two groups.Results The total effective rate in the observation group was significantly higher than the control group [90.70% (39/43) vs 67.44% (29/43)](P<0.05).The time of disappearance of lung rales, the disappearance time of cough, the time of fever and the recovery time of X -ray in the observation group were significantly shorter than the control group (P<0.05).The levels of serum hs-CRP, EC and SIL-2R in the observation group were lower than the control group (P<0.05) .There was no significant difference in adverse reaction rate between observation group and control group.Conclusion Bai Rui capsule combined with azithromycin in children with mycoplasma pneumonia can effectively reduce the serum hs-CRP, EC, SIL-2R levels, clinical efficacy is good, low adverse reaction rate.

BACKGROUND:It is unclearwhat kind of material for rotator cuff repair augmentation is the safest or most effective. OBJECTIVE:To review the basic research, clinical application and prospects of materials for rotator cuff repair augmentation. METHODS:Eligible studies were identified from electronic databases including EMbase, Medline, PubMed, OVID, Cochrane Library, Springerlink, CNKI, WanFang, and VIP. RESULTS AND CONCLUSION:There are four kinds of patches used for rotator cuff augmentation: tendon patches, non-degradable patches, extracelular matrix-based patches and degradable synthetic patches. Tendon patches have good mechanical strength, but postoperative foreign body reactions and increasing risk of infection and unable to recover the normal structure are problems to be solved. Non-degradable patches also have good mechanical strength, but the long-term safety is unclear. Extracelular matrix-based patches remain a lower mechanical strength and have a higher failure rate. Degradable synthetic patches are proposed to overcome these previous issues by combining wel-adjusted mechanical properties with biological additives and minimize risk of infection by completely absorbing in a time-dependent manner. However, migration of bioactive cels, regulation of degradation rate and suppression of acidic degradation products is are existing problems to be solved.

Objective To increase the radical resection rate and decrease local-regional relapse rate in thoracic esophageal carcinoma. Methods One-hundred and four patients were prospectively randomized to receive esophagectomy with thoraco-abdominal 2-field lymphadenectomy (48 cases) or with lymph node sampling (56 cases). In 101 patients who survived after surgery, 29 received adjuvant chemotherapy with cisplatin and fluorouracil (FP). Results Comparing with lymph node sampling, the operation time for lymphadenectomy was prolonged. However, there was no increase in blood loss, surgical morbidity, or mortality. Mean stations of lymph node dissected (10.5 stations vs. 3.2 stations,P

Objective To simulate local hemodynamic factors at particular arterial positions.Method A combination of high-lipid diet and immuoreactive injury was used to establish a hyperlipemia and atherosclerosis model in rabbit.Serrial sections were analysed by OLYSIA software.Data of caliber at different positions of carotid bifurcation,areas and circumference and thickness of the atherosclerotic plaque at carotid bifurcation were obtained and to establish a geometric model of rabbit carotid artery bifurcation with Gambit software.Wall shear stress distribution of the carotid sinus were analysed by numerical simulation.Result 1) A geometric model of rabbit carotid artery bifurcation was obtained.2) The wall shear stress of the carotid sinus of the atherosclerosis(AS) model group was found to be lower than that of the control group at shear rate 128.5 S~(-1).The lowest wall shear stress of the control was 4.028 times that of the AS model.Conclusion Low wall shear stress is a risk hemodynamic factor in the development of atherosclerotic plaque.

BACKGROUND:A few studies have reported that autologous chondrocyte implantation is better than microfracture for treating cartilage defects of the knee. But there are few meta-analyses on the clinical outcomes of autologous chondrocyte implantation versus microfracture. OBJECTIVE:To evaluate the effects of autologous chondrocyte implantation versus microfracture in the treatment of cartilage defects of the knee based on existing clinical data. METHODS:A systematic search for control ed clinical trials or control ed prospective observational studies published from 1979 to January 2015 was done in electronic databases MEDLINE, EMBASE, CINAHL, the Cochrane Central Register, Wanfang, CNKI and VIP. The literatures about the effects of autologous chondrocyte implantation versus microfracture in the treatment of cartilage defects of the knee were retrieved. We screened the retrieved literature according to the inclusion and exclusion criteria and performed a Meta-analysis with the software RevMan 5.2 after identification of the relevant data. RESULTS AND CONCLUSION:Eight studies were enrol ed according to the selection criteria, which revealed a statistical y significant difference, representing a clinical y relevant superiority of autologous chondrocyte implantation over microfracture, in IKDC scores at final fol ow-up [weighted mean difference (WMD),-9.93;95%confidence interval (CI):-13.16 to-5.43;P<0.000 01] and available scores at 5-year fol ow-up [standard mean difference (SMD),-0.30;95%CI: -0.55 to-0.05;P=0.02). In contrast, there were no significant differences, thus representing no clinical relevant superiority of microfracture versus autologous chondrocyte implantation, in Tegner scores at final fol ow-up (WMD=0.44;95%CI:0.04 to 0.84;P=0.03), Lysholm scores at final fol ow-up (WMD=-10.21;95%CI:-33.68 to 13.26;P=0.39), and available scores at 2-year fol ow-up (SMD=-0.25;95%CI:-0.92 to 0.43;P=0.47). These findings demonstrate that autologous chondrocyte implantation can result in a better long-term outcome than microfracgure. However, whether autologous chondrocyte implantation has a better treatment effect than microfracture in general needs more research.

ObjectiveTo investigate the effects of selective splenectomy on modulation of portal vein flow and prevention of small-for-size syndrome (SFSS) in living donor liver transplantation.MethodsTwenty six recipients who received LDLT from September 2007 to March 2008 were reviewed.The data of the portal vein flow of these recipients were collected during the operation.Simultaneous splenectomy was performed in patients with portal blood flow ＞250 ml/(min · 100g).No splenectomy was performed when the portal blood flow was less than 250 ml/(min · 100g).The effect of selective splenectomy on modulation of portal vein flow and whether splenectomy prevented the occurrence of SFSS were analyzed.ResultsThe portal vein flow decreased significantly after splenectomy in 8 patients who received splenectomy (P＜0.01),No SFSS occurred in the patients with or without splenectomy.Actual graft-to-recipient weight ratio (GRWR) of patients with splenectomy was significantly smaller than those with no splenectomy (P=0.044).The portal vein flow of patients with splenectomy was much higher than those with no splenectomy (P＜0.01).ConclusionAccording to the portal blood flow,selective splenectomy in LDLT decreased the portal vein flow and prevented the incidence of SFSS.

Objective To provide theoretical basis for clinical treatment of all-ceramic crown prosthodontics,the influence of cement thickness on the stress distribution in all-ceramic crowns under load was studied numerically.Methods The finite element analysis(FEA) model of a standard mandibular first molar was established using spiral CT and CAD software,and then it was applied to analyze the stress distribution of all-ceramic crowns under vertical loading using FEA software.Results Stress concentration was found in mesial and distal cement;the stress distribution at the cement upper and lower interface was found to be similar,and the stress magnitude discrepancy between the two interfaces was small;the stress of the model with 0.15 mm cement in the cervix was lower about 30% than the one of the 0.05 mm model.Conclusions The cement thickness plays an important role in the stress distribution of all-ceramic restoration system.The model with 0.15 mm cement shows a better resistance to destruction among the three models investigated.

ObjectiveTo investigate the effect of operation treating spinal multi-level tuberculosis.Methods45 patients with multi-level spine tuberculosis were treated with debridement completely and anterior or lateral-anterior intervertebrae autograft. Of them, 5 patients added to anterior instrumentation.Results45 patients had been followed up for 12 to 40 months.The back pain of 40 cases were relieved within 5 weeks after operation, erythrocyte sedimentation rate decreased 3 weeks after operation. 38 patients who involved in kyphosis decreased their kyphosis mostly and only one patient lost 3 degrees in follow-up.15 patients who involved in neurological deficits improved one or two grades (Frankel). Grafts fused in 44 patients and there were no recurrent in follow-up.Conclusions Operative treatment is efficacious to multi-level body spine tuberculosis.