The total glucosides of paeony root ( TCP ) extracted from paeony root of Boxian county, Anhui province was studied on the immunoregulatory effects in mice.The delayed hypersensitivity (DH) was increased by TGP at dose of 200mg/kg/d ig for 8d in the normal 6l5 mouse. Both depressed DH & enhanced DH induced by cyclophosph-amid ( Cy ) were restored by TGP ( 5 mg/kg/d ip x 5 - 8 d) in ICR mice. The decreased hemolysin antibody formation of spleen induced by Cy in BALB/c mice immunized with SRBC was raised by TGP ( 5mg /kg/d ip x 4d) . The phagocytic activity of ICR mice peritoneal macrophage on chiken RBC . In addittion, it was considerably Stimulated by using ip TGP at dose of 40mg/kg/d for 3d. The results suggested that TGP might possess the immunoregulatory effects in mice.

OBJECTIVE To emphasize the management of personnel and articles during operations with infective diseases,so to control the incidence of hospital infection.METHODS To establish an integrated and strict management of sterilization-isolation system,enhance the medical crew′s understanding of medical refuse′s harm during operations with infective diseases and intensify career training and supervising.RESULTS Hospital infection had been prevented effectively with procedure management and learning in operations with infective diseases.CONCLUSIONS The important procedures to prevent hospital infection are all medical crew take part in execution of perioperative antisepsis system strictly.

Aim To study IL-8 expression of U937 cells directly induced by Pseudomonas aeruginosa(PA)and its mechanism through the mitogen-activated protein kinase pathways.Methods The expressions of IL-8 mRNA in human monocytic leukemia cell lines(U937 cells)infected by Pseudomonas aeruginosa which were in differently differentiated and its protein secretion in the supernatant of culture medium were examined by the methods of RT-PCR and ELISA respectively.And these were then to be compared with those inhibited by MAPK inhibitors.Results Pseudomonas aeruginosa was able to induce IL-8 mRNA expression and their protein secretion both in U937 cells and in PMA differentiated U937 cells and had marked time and concentration dependent relations.Pretreament of U937 cells with specific inhibitors of ERK1/2(PD98059),the kinase that activated ERK1/2,p38(SB203580)could significantly diminished the PA-induced IL-8 production(P

For a preliminary study of the role of beta-catenin/Tcf signaling in squamous differentiation of airway (tracheobronchial) epithelial cells, a stable mutant of beta-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus. When co-transfected with Tcf luciferase reporter plasmids, beta-catenin mutant increased the reporter's transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein (SPRP), was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that beta-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
Cell Differentiation ; Cell Nucleus/metabolism ; Cells, Cultured ; Cornified Envelope Proline-Rich Proteins ; Cytoplasm/metabolism ; Epithelial Cells/cytology ; Epithelial Cells/*metabolism ; Membrane Proteins/metabolism ; Mutation ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Swine ; Trachea/cytology ; Trachea/*metabolism ; Transcription, Genetic ; beta Catenin/*metabolism

Triple-negative breast cancer (TNBC) is a heterogeneous disease.It has distinct risk factors,molecular biology features,clinical presentations and prognosis.TNBC recurrence is common after resection,and the survival rate is low and available treatment options are few after recurrence.To date,chemotherapy is the main treatment strategy for TNBC.There is a great need for new molecular predictive marks and drug targets for improving the efficacy of TNBC treatment.

Aim To study IL-8 expression of human airway epithelium-like NCI-H292 cells directly induced by Pyocyanin and its mechanism through protein kinase C(PKC) and nuclear factor-kappa B(NF-??).Methods ELISA methods were performed to test the expression of IL-8 in NCI-H292 cells infected by Pyocyanin.Western blot was employed to examine the expression of NF-?B protein.In addition,NCI-H292 cells were cultured together with PKC inhibitor,calphostin C or NF-?? inhibitor,pyrrolidine dithiocarbamate(PDTC),respectively before Pyocyanin infection,then the expression of IL-8 and NF-?? was assayed using the above methods.Results Pyocyanin was able to induce IL-8 protein secretion in NCI-H292 cells and had marked concentration-dependent relations.Pyocyanin could also significantly promote the activation of NF-?B,which peaked at 60~90 min.PDTC and calphostin C could significantly decrease the activation of NF-?? and the expression of IL-8.Conclutions Pyocyanin can induce IL-8 production.The direct induction of Pyocyanin can promote the activation of NF-?B by PKC signal pathway,then cause the expression and secretion of IL-8.

Objective To investigate the efficacy and adverse reactions of SOX and GP combined with two chemotherapy methods.Methods From January 2014 to September 2016 came to our hospital for treatment of three advanced breast cancer patients with negative 84 cases as the research object of observation,were randomly divided into SOX group and GP group were given chemotherapy measures and clinical observation on the treatment of.Results Two groups of patients with CR,PR,SD,PD,the number of cases and the total effective rate comparison,P values were 0.693,0.637,0649,0.801,0.823; two groups of patients with diarrhea,liver damage,thrombocytopenia,nausea and vomiting,neurotoxicity,anemia,leukopenia,rash,renal toxicity,appetite the incidence rate of decline,P values were 0.374,0.629,0.643,0.374,0.645,0.434,0.450,0.693,0.645,0.815.Conclusion Standard chemotherapy regimens for advanced three negative breast cancer was not uniform,but the SOX and GP regimens are safe and effective,can be used for clinical treatment.

AIM: To investigate the inhibitory effect of arsenic trioxide on human glioma cell line U251 growth, change of gene expression and intracellular calcium content. METHODS: MTT method was used to observe the growth inhibition effect. Cell cycle, positive rate of proliferation cell nuclear antigen (PCNA), apoptosis associated protein Fas and Bcl-2, and intracellular calcium ion (IECa~ 2+ ) levels were measured by flow cytometry in U251 cells treated with different doses of As_2O_3. Apoptosis was detected with annexin V-FITC+PI dual parameter. RESULTS: As_2O_3 inhibited the growth of U251 cells dramatically. There were obvious dosage-effect and time-effect correlations (P0.05). The cell cycle was arrested in G_2M phase. Apoptosis occurred in U251 cells treated with As_2O_3 by annexin V-FITC+PI dual parameter detection. CONCLUSION: As_2O_3 inhibits the growth of U251 cells in vitro dramatically and induces apoptosis. The mechanism is probably associated with the improvement of Fas expression and IECa~ 2+ levels, decrease in PCNA protein expression and cell cycle arrest.

OBJECTIVE: To evaluate the clinical efficacy of Cefepime in treating senile patients with hospital acquired pneumonia(HAP).METHODS: A total of 60 HAP cases were randomly assigned to receive Cefepime(n=30) or Ceftazidime (n=30) for 7～10 days.The effective rate and the bacterial clearance rate of two groups were compared.RESULTS: The effective rate was 86.7% for Cefepime group and 70.0% for Ceftazidime group(P