ObjectiveTo investigate the effect of hyperglycemia on calcium load and cyclic AMP content in normal and STZdiabetic rat skeletal muscle.MethodsCalcium load and cyclic AMP content were measured in vivo and in vitro after incubation with 5.6mmol/L and 16.7mmol/L glucose concentration.Atom spectrum absorption assay for calcium and radioimmunoassay for cAMP were employed.ResultsHigh concentration glucose elevated cyclic AMP content and lessened calcium load significantly.Cyclic AMP also elevated in diabetic skeletal muscle incubated with even 5.6mmol/L glucose.Diabetes was necessary for high concentration glucose to elevate cyclic AMP in vitro,because cyclic AMP did not change in normal rat muscle even incubated with 16.7mmol/L glucose.But diabetes discounted the reduction of calcium load induced by 16.7mmol/L glucose.ConclusionThe effects of hyperglycemia on calcium load and cyclic AMP content in skeletal muscle may accord with its antiinsulin action,which may be involved in the mechanism of glucose toxicity.

221 subjects at risk for MS were enrolled and evaluated.The discovered mean number of MS components was little greater by IDF definition than by CDS one(2.33 vs 2.09,P>0.05).The concordance rate of both definitions was 80% in diagnosing MS.The female MS prevalence was higher by IDF (18/83) than by CDS (2/83)(P<0.05).But male MS prevalence was higher by CDS (18/138) than by IDF (7/138) (P<0.05).In those with normal glycaemia, more individuals were diagnosed to have MS by IDF definition (21/94,22.3%) than by CDS one (10/97,10.3%)(P<0.05).Of people with MS diagnosed on CDS definition, 11 (12%) were non-obese.Finally, 6% MS people was missed by IDF definition due to neglecting postprandial-challenge glycemia.In conclusions, the two definitions are comparable in identifying MS and its components, while from CDS to IDF definition some MS people drift in the way as follows: from men to women, from dysglycemia to abnormal fat metabolism, and from non-obesity to obesity.

Objective To analyze the CT and MRI features of pituitary stalk interruption syndrome, so that to improve the diagnostic knowledge of this disease.Methods The imaging and clinical data of 4 patients with pituitary stalk interruption syndrome were retrospectively analyzed. Results In all of the 4 cases, the pituitary stalk were not shown by CT in 2 and by MRI in 4,the high signal intensity of the posterior lobe disappeared on T1WI,while a small nodule in infundibulum with high signal intensity on T1WI was found. Conclusion MRI is the most valuable imaging modality for the diagnosis of pituitary stalk interruption syndrome.

Objective To investigate the relationship of glycaemia level and the artery stiffness by means of ultrasonography. Methods Among the individuals selected for screening for impaired glucose tolerance, the systolic and diastolic diameters of both common carotid arteries in a single cardiac cycle, and the intima-media thickness were measured. Based on these measured parameters the artery compliance (AC), stiffness index and Yang's elastic Model (YEM) were calculated. Both fasting blood sugar level and that of 2 hours after 75g glucose challenge were quintiled so as to compare the arteriosclerosis surrogates. Linear correlation and partial linear regression were used to analyze the relationship of both fasting and post-challenge blood sugar levels with the three surrogates. Finally, those surrogates were also compared among different glucose tolerance categories. Normal glucose tolerance (NGT, n=97), impaired glucose tolerance (IGT, n=51) and type 2 diabetes mellitus (T2DM, n=73) were classified by the previous results of 75g oral glucose tolerance test. Results The AC decreased, and SI and YEM elevated significantly after the third quintile of fasting glycaemia, while the similar changes were found after the fourth quintile of 2 hour glycaemia. Both fasting and post-challenge glycaemia correlated inversely with AC and positively with SI and YEM. Partial correlation analysis showed that the significance of the correlation of fasting glucose with the arteriosclerosis surrogates disappeared but that of post-challenge glucose with those surrogates remained. In different categories of glucose tolerance, AC decreased but YEM rose significantly in IGT and T2DM when compared with NGT. The SI of T2DM was higher than of NGT. However the three surrogates remained similar between IGT and T2DM. Conclusions Artery stiffness was enhanced with the increase in fasting or post-challenge glycaemic levels. Post-challenge glycaemia was a better determinant than fasting glycaemia. The degree of arteriosclerosis in IGT was higher than that in NGT, but was similar to that of T2DM.

本文在“国际残疾分类”的基础上，围绕康复诊断名称的标准化，进行了初步探讨，力求使之适合于临床康复的应用及康复病案管理。

Objective To examine the effects of different doses of midazelam on ED50 of emulsified isoflurane and to determine the type of interaction between them for hypnosis by isobelographic analysis in rats. Methods One hundred and twenty-five adult male SD rats weighing 240-300 g were randomized into 5 groups (n=25 each): group Ⅰmidazolam (M); group Ⅱ emulsified isnflurane (Ⅰ); group Ⅲ ,Ⅳ ,Ⅴ, 1/4, 1/2 and 3/4 ED50 of midazelam for hypnosis + emulsified isoflurane (MI1 , MI2, MI3). Up-and-down sequential experiment was used to determine ED50of midazolam and emulsified isoflurane for loss of righting reflex in group Ⅰ -Ⅴ . The intial dose of midazolm was 17.3 mg/kg in group M. The initial doses of emulsified isoflurane (120 mg/ml) were 0.55 (in group Ⅰ, 0.22 (MI1), 0.19 (MI2) and 0.12 ml/kg (MI3) respectively. In group MI1-3 midazolam was injected over 15 seconds and after an interval of 2.5 min emulsified isoflurane was injected over 10 s. ED50 was calculated using Dixon-Mood method. Isebolographic and algebraic analyses were used to determine the type of interaction between midazolam and emulsified isoflurane. Results The five groups were comparable with respect to M/F sex ratio and body weight. The Edso of midazolam was 26.0 mg/kg in group M. Midazolam 6.5, 13.0 and 19.5 mg/kg were given in group MI1 , MI2 and MI3 respectively. The ED50 of emulsified isoflurane was 0.67 (ingroup Ⅰ), 0.30 (MI1), 0.22 (MI2) and 0.18 ml/kg (MI3) respectively. The isobolographic analysis indicated that with increasing doses of midazolam, the Edw of emulsified isoflurane decreased progressively in a non-linear fashion. The isobolographic and algebraic analyses demonstrated that the interaction between midazolam and emulsified isoflurane was synergistic for hypnosis. Conclusion The hypnosis is synergistic when midazolam 6.5,13 mg/kg are combined with emulsified isoflurane and additive when midazolam 19.5 mg/kg is combined with emulsified isoflurane.

Objective To investigate the effect of butylphthalide on the memory,movement and ERP (event-related potentials) in patients with cerebral infarction cognitive impairment.Methods A total of 134 patients with cerebral infarction cognitive impairment were randomly divided into treatment group (67 patients) and control group (67 patients).All patients were given conventional cerebral infarction treatment as well as cognitive improvement intervention.Oral administration of butylphthalide was added onto the patients in the treatment group.Curative effects were observed after 30 days′ continued treatment.The cognitive function scores,memory scores,movement scores,ERP test and adverse reaction were compared between the two groups before and after treatment.Results After 30 days′ treatment,two groups of the montreal cognitive assessment scale (MoCA) scores were 27.2±4.9 and 25.1±4.1 respectively;the total scores of memory were 5.5±0.5 and 4.9±0.5 respectively;the scores of motor assessment scale (Fugl-meyer) were 85.6±6.2 and 74.2±6.1 respectively and P3 amplitudes were (9.5±0.9)μV and (8.1±0.9)μV respectively.All the indexes mentioned above in treatment group were significantly increased compared with those of before treatment(P<0.05).The results of N1,N2,P2 and P3 in the latency detection were significantly decreased compared with those of before treatment(P<0.05).And the results of P2 and P3 in the latency detection of the treatment group were shorter than those of the control group(P<0.05).After treatment,no adverse reaction was observed in both groups.Conclusion Based on conventional cerebral infraction treatment,oral administration of butyraldehyde effectively improve the therapeutic effect.

OBJECTIVE:To investigate whether the magnesium sulfate injection(MSI)into cisterna magna in a rabbit with subarachnoid hemorrhage(SAH)can reverse the cerebral vasospasm and damage of brain tissues.METHODS:The single-hemorrhage SAH rabbit model was used.Thirty New Zealand white rabbits were randomly divided into the following three groups(n=10,in the each group):sham group,model group and MSI group.At 24 hours after SAH,the rabbits were injected with 0.1 mL?kg-1 of saline into cisterna magna in sham and model groups,while 0.1 mL?kg-1 of 4% magnesium sulfate was injected into cisterna magna of rabbits in MSI group.All animals were sacrificed at 48 hours after SAH.Basilar arteries and hippocampus were then removed for measurement of the cross-sectional areas of basilar arteries and the hippocampus normal neuron density of CA1 regions.RESULTS:The cross-sectional areas of basilar artery and the hippocampus normal neuron density of CA1 regions were smaller in model group than in the other two groups(P0.05).CONCLUSIONS:This study results indicate that injecting magnesium sulfate into cisterna magna can reverse the cerebral vasospasm and the following hippocampal neuron damage in rabbits with subarachnoid hemorrhage.

Immunoglobulins ( Ig ) , also called antibodies, are important components in humoral -mediated immunity. Ig can bind with their receptors, called immunoglobulin receptors ( IgR ) , trigger biologic activities respectively. Different sub-types of Igs show different function. And IgRs have been treated as therapeutic targets in inflammation and immunity related dis-eases for many years. This article reviewed the recent progresses in the study of IgR function and its therapeutic role in inflamma-tion and immunity related diseases.