Objective To explore the functional sensitivity ,maximum allowable dilution ,analytical measurement range and clin‐ical reportable rang of the serum alanine aminotransferase (ALT ) detected by Beckman Dxc800 automatic analyzer with the DiaSys diagnostic biochemistry reagent kits .Methods According to the EP6‐A document published in 2004 by American Clinical and La‐boratory Standards Institution (CLSI) ,these samples with equal interval ,proportional prepared with the low levels serum and the high levels serum and their concentration exceeding the range claimed by manufacture ,were detected twice each .The results were registered and analyzed by the SPSS19 .0 statistic software with polynomial regression analysis to determine the analytical measure ‐ment range .Five samples at the low concentration ,which were prepared previously ,were detected repeat at day interval .And the functional sensitivity was determined with combining the coefficient of variation and the average value .Three samples ,diluted to different dilution ratio with physiological saline ,were detected .The dilution recovery rate ,which was required in the range of 90%- 110% ,was calculated to establish the clinical reportable rang by combining the Functional Sensitivity .Results The analytical measurement range of the serum ALT detected by Beckman Dxc800 analyzer was 5 .50 - 847 .50 U /L ,the runctional sensitivity was 3 .698 U /L ,the maximum allowable dilution was 1 ∶ 8 and the clinical reportable rang was 3 .698 - 6 780 .0 U /L .Conclusion The analytical measurement range of the serum ALT detected by Beckman Dxc800 analyzer with the DiaSys diagnostic biochemistry rea‐gent kits ,was wider than that of being claimed by manufacture ,the clinical reportable rang could meet the need of clinic .

Objective To study on the relationship between the levels of homocysteine(HCY) ,folic acid and vitamin B12 and pregnancy-induced hypertension(PIH) in different pregnancies .Methods 539 pregnant women who registered for prenatal exami-nation of pregnant in the hospital were selected as research subjects .And there were 87 cases of PIH(PIH group) and 452 cases of normal pregnancy(normal pregnancy group) among them .The fasting blood samples were collected respectively in early pregnancy (8-10 weeks and 12-14 weeks of pregnancy) ,mid pregnancy(18 pregnancy weeks and 24 pregnancy weeks) ,and late pregnancy (30 pregnancy weeks and 36 pregnancy weeks) ,and the levels of HCY ,folic acid and vitamin B12 were measured .At the same time ,the supplements of folic acid and vitamin B12 and the incidence of PIH and birth defects were asked ,registered and checked . Results Compared with normal pregnancy group ,the serum HCY level of PIH group significantly increased in medium and late pregnancy periods (P<0 .05) ,and had no statistical significance in early pregnancy(P>0 .05) .In mid and late pregnancy periods , the serum HCY levels of PIH group and normal pregnant group negatively correlated with serum folic acid levels (r<0 ,P<0 .05) , and did not correlate with vitamin B12 levels (P>0 .05) .Conclusion In middle and late pregnancy periods ,if the serum HCY level of pregnant women increased ,the risk of PIH increased significantly .