Obiective To explore the application of psycho-cardiology in patients with chronic heart failture compli-cated with depressive disorder. Methods Hospitalized patients with chronic heart failture were assessed with PHQ-9 and PHQ-2 health questionnaire. A total of 80 patients with depressive disorder were selected and randomly divided into routine treatment group (n=40, received routine medication) and intervention group (n=40, application of psycho-cardiology based on routine medication). After three months, changes of scores before and after therapy were statistically analyzed in these two groups. Results Compared with routine treatment group, total scores of PHQ-9 significantly decreased in intervention group (P＜0.01). LVEF, BNP and CRP improved in both groups after intervention (P＜0.05), which were more prominent in intervention group compared with routine treatment group (P ＜ 0.05). Conclusion Psycho-cardiology can treat chronic heart faiture and solve psychological problems of patients and provide comprehensive rehabilitation to patients.

Background The pathology of choroidal neovascularization (CNV) is not clear.CD147 plays a critical role in angiogenesis through activating matrix metalloproteinases (MMPs) and degradation of extracellular matrix (ECM).However,the study of CD147 in CNV is rare.Objective This study was to investigate the expression of CD147 and vascular endothelial growth factor (VEGF) in laser-induced rat CNV model.Methods Thirty clean BN rats were divided into normal control group (six rats) and laser group (24 rats) according to the random number table.CNV was induced on laser photocoagulation group rats using 532 nm multiple-wave length photocoagulation.The development of CNV was followed up by fundus fluorescence angiography (FFA) on 1 day,7,14 and 21 days post-photocoagulation.The expression of C D147 protein in retinal pigment epithelium (RPE)-choroidsclera complex was examined by Western blot in normal control group and 1 day,7,14,21 days post-photocoagulation group.The vitreous VEGF concentrations of those groups were detected by ELISA.Results Disk like fluorescein leakage was detected on 7 days after photocoagulation,and CNV was formed on 14 days after photocoagulation.The expressions of CD147 protein were 1.00 ± 0.43,0.97 ± 0.53,0.99 ± 0.45,1.56 ± 0.67 and 2.27 ± 0.54,and the concentrations of vitreous VEGF were (72.96±29.95),(79.36±10.46),(103.82±32.94),(166.05±21.54) and (195.64± 39.90)pg/ml in normal control group and 1 day,7,14,21 days post-photocoagulation group,with significant differences among the groups (CD147:F=10.95,P＜0.01;VEGF:F=304.50,P＜0.01).The expressions of CD147 and VEGF were significantly higher in the 14,21 days post-photocoagulation groups than those in the normal control group (all at P＜0.05).Conclusions The expression of CD147 increases as the induction of CNV,in accordance with the expression of VEGF.CD147 may play an important role in the early development of CNV.

Objective:To evaluate the effect of resveratrol on ferropotosis in cardiomyocytes of mice with diabetic cardiomyopathy.Methods:Thirty healthy adult male C57BL/6 mice, aged 8 weeks, weighing 22-26 g, were divided into 3 groups ( n=10 each) using a random number table method: control group (group C), diabetic cardiomyopathy group (group DCM) and resveratrol group (group RSV). Freshly prepared streptozotocin (STZ) 40 mg·kg -1·d -1 was intraperitoneally injected for 5 consecutive days to develop the model of type 1 diabetes mellitus. After the model was successfully developed, resveratrol 25 mg·kg -1·d -1 was intragastrically given for 12 consecutive weeks in group RSV, while the equal volume of dimethyl sulfoxide was given instead in group C and group DCM. Echocardiography was performed to examine the cardiac structure and function at the end of the 12th week. Then mice were sacrificed, and myocardial tissue specimens were harvested for microscopic examination of the pathological changes of myocardial tissues (by Hematologist-Eosin staining) and mitochondrial morphology of myocardial cells (with a transmission electron microscope) and for determination of the contents of iron, malondialdehyde (MDA) and glutathione (GSH) (by colorimetry) and expression of glutathione peroxidase 4 (GPX4) (by Western blot). Results:Compared with group C, the left ventricular end-diastolic diameter and left ventricular end-systolic diameter were significantly increased, the left ventricular ejection fraction and left ventricular fractional shortening were decreased, the contents of iron and MDA were increased, the content of GSH was decreased, and the expression of GPX4 was down-regulated in group DCM ( P<0.05). Compared with group DCM, the left ventricular end-diastolic diameter and left ventricular end-systolic diameter were significantly decreased, the left ventricular fractional shortening and ejection fraction were increased, the contents of iron and MDA were decreased, the content of GSH was increased, the expression of GPX4 was up-regulated ( P<0.05), and the pathological changes of myocardial tissues and changes in mitochondrial morphology of myocardial cells were significantly attenuated in group RSV. Conclusions:The mechanism by which resveratrol attenuates myocardial injury and further improves cardiac dysfunction is related to inhibition of ferroptosis in cardiomyocytes of mice with diabetic cardiomyopathy.