Objective To investigate the protective effective mechanism of Fleabane on PC12 cells impaired by hypoxia-reoxygention (HR).Methods The PC12 cells cutured in vitro were grouped as following:normal control (NC) group, the HR group,Fleabane 10?mol/L (E1)group, 20?mol/L (E2)group, 30?mol/L (E3)group. The livability of PC12 was measured after impaired by HR in groups HR and E1～E3. The expressions of Bcl-2 and Bax were also detected by Western Blot. Results (1)Compared with NC group,the survival rate of PC12 decreased in HR group(P

Objective To analyze the influencing factors of vaginal birth after cesarean (VBAC) in re-pregnancy puerperae and mother-infant outcomes.Methods A total of 290 re-pregnant women after cesarean were enrolled in the study,and 210 pregnant women were successfully given vaginal delivery,among whom 180 cases with vaginal birth were selected as the observation group,and 80 cases with cesarean section were as control group.The factors affecting the success of vaginal delivery after cesarean section were analyzed,and the maternal and neonatal outcomes between the two groups were compared.Results The univariate analysis showed that fetal head position,fetal abdominal circumference,fetal biparietal diameter,uterine height,premature rupture of membranes,Bishop score,and epidural anesthesia of two groups were statistically significant(P ＜ 0.05);Multivariate logistic regression analysis showed that Bishop score,fetal abdominal circumference,fetal head position,and epidural anesthesia were independent influencing factors of VBAC success (P ＜ 0.05).The incidence rates of uterine rupture,the rate of neonatal asphyxia,hospitalization rate,Apgar score of two groups showed no significant difference (P ＞ 0.05).The bleeding rates at 2,24 h in the observation group were lower than that in the control group (P ＜ 0.05).Conclusion There are various factors that affect the success of vaginal delivery after cesarean section,and prenatal examination should be scientifically evaluated.If there is no indication of cesarean section,maternal vaginal delivery should be guided to improve the maternal and child outcomes and life quality of mother and child.

Objective To analyze the influencing factors of vaginal birth after cesarean (VBAC) in re-pregnancy puerperae and mother-infant outcomes.Methods A total of 290 re-pregnant women after cesarean were enrolled in the study,and 210 pregnant women were successfully given vaginal delivery,among whom 180 cases with vaginal birth were selected as the observation group,and 80 cases with cesarean section were as control group.The factors affecting the success of vaginal delivery after cesarean section were analyzed,and the maternal and neonatal outcomes between the two groups were compared.Results The univariate analysis showed that fetal head position,fetal abdominal circumference,fetal biparietal diameter,uterine height,premature rupture of membranes,Bishop score,and epidural anesthesia of two groups were statistically significant(P ＜ 0.05);Multivariate logistic regression analysis showed that Bishop score,fetal abdominal circumference,fetal head position,and epidural anesthesia were independent influencing factors of VBAC success (P ＜ 0.05).The incidence rates of uterine rupture,the rate of neonatal asphyxia,hospitalization rate,Apgar score of two groups showed no significant difference (P ＞ 0.05).The bleeding rates at 2,24 h in the observation group were lower than that in the control group (P ＜ 0.05).Conclusion There are various factors that affect the success of vaginal delivery after cesarean section,and prenatal examination should be scientifically evaluated.If there is no indication of cesarean section,maternal vaginal delivery should be guided to improve the maternal and child outcomes and life quality of mother and child.

Objective:To investigate the incidence of venous thromboembolic disease (VTE) in urological inpatients during perioperation.Methods:The clinical data of 7 988 inpatients admitted to the Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology from January to December 2019 were analyzed retrospectively. The cohort included 5 657 males and 2 331 females. The average age of the enrolled patients was (56.3±15.8) years old, and the body mass index was (23.8±3.2) kg/m 2. There were 1 628 malignant tumors patients and 6 360 non-malignant tumors patients in the cohort. Of all the patients, 7 725 cases received surgical treatment. All patients were scored with the Caprini Risk Assessment Model (2005) after admission and 1 655 cases were classified as very low risk, 2 940 cases were low risk, 2 922 cases were medium risk, 345 cases were high risk and 126 cases were very high risk. Patients with Caprini score ≥ 2 and/or clinical symptoms were examined by venous color Doppler ultrasound. CT pulmonary angiography was performed for patients with chest pain, chest tightness, decreased blood oxygen saturation and other symptoms suspected of pulmonary embolism according to clinical judgment to screen the incidence of VTE. Results:Among the 7 988 cases, 180 cases (2.25%, 180/7 988) with VTE were found by preoperative examination, including 1 case (0.01%, 1/7 988) with pulmonary embolism. There were 199 new cases with VTE after operation, and the incidence of new VTE after operation was 2.58% (199/7 725). Among them, pulmonary embolism was found in 7 cases, with a incidence of 0.09% (7/7 725). Only 7.92% (30/379) of the VTE patients had VTE-related symptoms. The operations with higher incidence of VTE were radical cystectomy, nephroureterectomy, radical prostatectomy and radical nephrectomy, with the incidence of 11.61% (13/112), 10.87 (10/92), 8.25% (16/194) and 6.16% (22/357) respectively.Conclusions:The incidence of VTE in hospitalized patients with urinary surgery in this study is much higher than previously reported. Most of the patients with VTE are asymptomatic. The operations with high incidence of VTE after operation are radical cystectomy, nephroureterectomy, radical prostatectomy and radical nephrectomy. Therefore, it is necessary to carry out VTE screening for hospitalized patients in urology department, which is helpful to realize early intervention of VTE and reduce the risk of VTE progression and pulmonary embolism.

Objective To explore the effects of the teaching model of "multi-subject cooperation integration" in training of independent learning ability among student nurses. Methods Two classes of four-year undergraduate nursing from a school of nursing in a university were selected as subjects by purposive sampling from September 2014 to January 2016. By lottery, class A (94 students) acted as observation group, while class B (94 students) acted as control group. Control group carried out routine teaching according to teaching program. On the basis of routine teaching, observation group developed the teaching model of"multi-subject cooperation integration" in sectional chapters of three major courses of nursing speciality. The independent learning ability was evaluated with the self-rating scale for self-directedness in learning (SRSSDL). Results After educational reform, the total score of SRSSDL and the scores of learning activities, evaluation of observation group were (230.21±24.75), (43.95±5.39), (45.97±5.95) higher than those [(222.60±24.24), (42.35±5.67), (43.06±5.78)]of control group with significant differences (t=0.033, 0.047, 0.001; P<0.05). Conclusions The teaching model of "multi-subject cooperation integration" can improve the independent learning ability of student nurses.

Objective:The aim of this study is to investigate the relationship between fat mass and obesity associated ( FTO) gene polymorphism and the risk of gestational diabetes mellitus (GDM), and provide clues and basis for the study of GDM mechanism. Methods:The case group of GDM pregnant women who delivered at the First Affiliated Hospital of Shanxi Medical University from March 1, 2012 to July 30, 2014 were selected, and matched the control group among non-GDM pregnant women by age, gestational age and residential address, and 324 cases and 318 controls were finally included. DNA was extracted and genotyped, and min P test and unconditional logistic regression model were used to estimate the relationship between FTO gene polymorphism and GDM. Results:At gene level, we did not find the association between FTO and the risk of GDM ( P>0.05). After adjusted for family history of diabetes, pre-pregnancy body mass index and multiple comparisons using false discovery rate method, unconditional logistic regression analysis showed that pregnant women who carried the rs11075995 TT genotype ( OR=0.59, 95 %CI: 0.35-0.89), rs3826169 GG genotype ( OR=0.59, 95 %CI: 0.35-0.88), and rs74245270 GA genotype ( OR=0.69, 95 %CI: 0.49-0.98), GA or AA genotype( OR=0.70, 95 %CI: 0.50-0.97) had reduced risk of GDM. However, pregnant women who carried the rs74018601 GA genotype ( OR=1.51, 95 %CI: 1.07-2.12), GA or AA genotype ( OR=1.46, 95 %CI: 1.06-2.02), rs7205009 AA genotype ( OR=1.83, 95 %CI: 1.18-2.86), GA or AA genotype ( OR=1.53, 95 %CI: 1.08-2.19), and rs9888758 AG genotype ( OR=1.43, 95 %CI: 1.02-2.00) had elevated risk of GDM. Conclusion:The polymorphisms of FTO gene rs11075995,rs3826169, rs74245270, rs74018601, rs7205009 and rs9888758 were associated with the risk of GDM.

Objective:To investigate the relationship between folic acid supplementation and the risk of preeclampsia (PE).Methods:A total of 9 048 pregnant women were selected from the First Hospital of Shanxi Medical University in Taiyuan from March 2012 to September 2016. Among them, 882 pregnant women with PE were divided into case group, and 8 166 pregnant women without PE were divided into control group. Information on demographic characteristics, folic acid supplementation, maternal complications, and other factors were collected by face-to-face interviews after child birth in the hospital. Unconditional logistic regression analyses were used to investigate the relationship between folic acid supplementation and the risk of PE and the effects of pre-pregnancy BMI on the relationship of folic acid supplementation with the risk of PE.Results:Compared with nonusers, folic acid supplement users had reduced risk of PE ( OR=0.79, 95 %CI: 0.64-0.96). Folic acid supplementation before and during pregnancy were negatively related with the risk of PE ( OR=0.63, 95 %CI: 0.49-0.81). Pregnant women who used folic acid tablets only or used both folic acid tablets and multivitamin containing folic acid had reduced risk of PE ( OR=0.81, 95 %CI: 0.66-0.99; OR=0.64, 95 %CI: 0.49-0.85). No significant relationship was observed in the multivitamin group. Supplemental folic acid doses of <400, 400, and >400 μg/d were related with reduced risk of PE ( OR=0.62, 95 %CI: 0.42-0.91; OR=0.81, 95 %CI: 0.66-0.99; OR=0.68, 95 %CI: 0.49-0.94). After stratified by pre-pregnancy BMI, pregnant women who used folic acid supplementation, those with pre-pregnancy BMI<24.0 kg/m 2 had reduced risk of PE ( OR=0.75, 95 %CI: 0.59-0.96). However, no significant relationship was observed in women with pre-pregnancy BMI≥24.0 kg/m 2. Conclusions:Folic acid supplementation before and during pregnancy were related with reduced risk of PE. Pre-pregnancy BMI might affect the relationship between folic acid supplementation and the risk of PE. Appropriate folic acid supplementation should be recommend for women with different pre-pregnancy BMI.

Objective: To investigate the relations between dietary intake during pregnancy and the incidence of their babies with small for gestational age (SGA). Methods: Data on demographics, dietary intake of protein, fat, and carbohydrates of the pregnant mothers during the first, second and third trimester, were collected. Information related to birth weight and gestational age of the infants were also gathered. A total of 8 102 women, who delivered their babies at the First Affiliated Hospital of Shanxi Medical University from March 2012 to September 2016, were enrolled in this project. Among them, 961 mothers had infants with SGA but the other 7 141 of them having normal infants. Unconditional logistic regression model was used to analyze the effect of dietary nutrient intake on SGA the first, second and third trimester. Results: We found that low dietary intake of protein during the first trimester and following trimesters during pregnancy were positively associated with higher risk of SGA (OR=1.534, 95%CI: 1.217-1.934; OR=1.268, 95%CI: 1.005-1.599; OR=1.310, 95%CI: 1.036-1.655). When adjusting for maternal pre-pregnancy BMI, we found that when mothers were with a pre-pregnancy BMI less than 18.5 or with low maternal intake of protein during the first trimester, positive association with higher risk of SGA (OR=1.872, 95%CI: 1.033-3.395; OR=1.754, 95%CI: 1.125-2.734), was noticed. However, for mothers with a pre-pregnancy BMI between 18.5 and 24.0 or with low protein intake during the first trimester, significant association with higher risk of SGA (OR=1.465, 95%CI: 1.089-1.972) was found. Conclusions Through our observation, maternal dietary intake during pregnancy seemed to be associated with the risk of SGA but the effects of dietary intake were different, according to the BMI of pre-pregnancy population. Early pregnancy appeares as the key period for dietary intake which may influence the SGA.

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms. Current CRISPR-activation (CRISPRa) platforms often require multiple components because of inefficient transcriptional activation. Here, we fused different phase-separation proteins to dCas9-VPR (dCas9-VP64-P65-RTA) and observed robust increases in transcriptional activation efficiency. Notably, human NUP98 (nucleoporin 98) and FUS (fused in sarcoma) IDR domains were best at enhancing dCas9-VPR activity, with dCas9-VPR-FUS IDR (VPRF) outperforming the other CRISPRa systems tested in this study in both activation efficiency and system simplicity. dCas9-VPRF overcomes the target strand bias and widens gRNA designing windows without affecting the off-target effect of dCas9-VPR. These findings demonstrate the feasibility of using phase-separation proteins to assist in the regulation of gene expression and support the broad appeal of the dCas9-VPRF system in basic and clinical applications.
Humans ; Transcriptional Activation ; RNA, Guide, CRISPR-Cas Systems ; Gene Expression Regulation ; CRISPR-Cas Systems/genetics*