OBJECTIVE:To establish a quality standard for Shenning tablet.METHODS:Leonurus japonicus,Radix et Rhizoma Rhei,Gardenia jasminoides,Phellodendron chinense in Shenning tablet was identified by TLC.The contents of Jasminoidin and Peoniflorin in Shenning tablet were determined by HPLC.RESULTS:The relevant spots in Leonurus japonicus,Radix et Rhizoma Rhei,Gardenia jasminoides,Phellodendron chinense were identified by TLC.The linear ranges of Jasminoidin and Peoniflorin were 0.032 2～0.386 4 ?g(r=0.999 9) and 0.041～0.492 ?g(r=0.999 9),respecctively,with average recovery rates at 101.09% and 100.08%,respectively and RSD at 0.54% and 1.28%,respectively(n=6).CONCLUSION:The established quality standard is suitable for the quality control of Shenning tablet.

Objective To observe the effects of resistant starch (RS) on insulin resistance (IR) in type 2 diabetes mellitus rats. Method Male Wistar rats (n=40) were used.Ten were fed with normal diets (normal control group) and the others with high fat (safflower oil, 59% of total energy) diet (model group) . Ten weeks laters, the model group was injected with streptozotocin (20mg/kg bw) and divided into 3 groups: diabetes control group, high fat and RS intervention group, RS intervention group. After 5 w, oral glucose tolerance test (OGTT) was carried out in each group and the metabolism of glucose and lipid, insulin sensitivity, pathological changes of liver and kidney were observed. Results Compared with diabetes control group, insulin sensitivity index (ISI) was higher and area under curve (AUC), fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), low density-lipoprotein cholesterol (LDL-C) were significantly lower in RS intervention groups. Besides, the fatty degeneration of liver was much lighter but glycogen synthesis was significantly increased. Conclusion RS is effective in improving IR of type 2 diabetes mellitus rats.

BACKGROUND:An increasing number of studies have shown that mesenchymal stem cels have the potential to treat acute kidney injury. Umbilical cord-derived mesenchymal stem cels have general characteristics of stem cels and many advantages, such as easy to isolate and culture, in vitrofast amplification, low immunogenicity and no ethical problems, which have garnered increasing attentions. OBJECTIVE:To study the repairing effects of human umbilical cord-derived mesenchymal stem cels on acute kidney injury in rats. METHODS: Thirty rats were randomized into three groups: a normal control group, a model group and a cel transplantation group. Rats in the model and cel transplantation were subjected to clamping the renal pedicles for 45 minutes, and then injected 1 mL of DAPI-labeled umbilical cord-derived mesenchymal stem cels or 1 mL of saline via the tail vein. In the normal control group, the kidney was only exposed with no treatment. At 7 days after treatment, the rats were kiled to take left kidney tissues for pathological observation under light microscope and right kidney for observation of DAPI-positive cel counting. Automatic biochemical analyzer was used to detect serum creatinine and urea ammonia levels. RESULTS AND CONCLUSION: Compared with the model group, the levels of serum creatinine and urea ammonia were significantly lower in the cel transplantation group (P < 0.05), suggesting that human umbilical cord-derived mesenchymal stem cels can improve the kidney function to a certain extent. Pathological findings showed that the pathological damage was improved more remarkably in the cel transplantation group than the model group, and the tubular necrosis index decreased significantly in the cel transplantation group. At 7 days after cel transplantation, blue fluorescent cels were scattered on renal tissue frozen sections. These results indicate that human umbilical cord-derived mesenchymal stem cels can migrate to the injured tubular epithelial tissues, and promote the repair of the injured kidney.

Objective To explore the relationship between polymorphism of angiotensin converting enzyme (ACE) gene and vascular dementia (VD).Methods 94 cases of VD, 60 cases of essential hypertension (EH) and 60 healthy controls were enrolled to analyze insertion/deletion polymorphism of ACE gene by polymerase chain reaction (PCR).Results The DD genotype and the frequency of D allele were significantly higher in VD patients than in healthy controls (all P

Cancer molecular epidemiology focus on distribution and pathogenesis of tumor markers in high-risk populations and cancer patients.Cancer molecular epidemiology tries to open the "black box" and to elucidate the nature of cancer through the measurement and evaluation of exposure and response to carcinogens and individual genetic susceptibility,involving the fields of etiology,preventive medicine and clinical medicine.We need rigorously strengthen the design and implementation of cancer molecular epidemiology,the research and application of TM should enhance the principles of quality,evidence and ethics.With the utility of new subject tools such as proteomics,cancer molecular epidemiology which combines the advantages of multi-subjects is now quickly changing.

Objective:The glucemic index(GI)and insulin index(II)of wheat flour, oat fiber and resistant starch (RS) were studied.Method:Ten healthy subjects consumed 4 kinds of carbohydrate foods: glucose powder,wheat flour food, oat fiber food and RS food.Up to 120 min after consumption of test materials, blood samples were taken for glucose and insulin analyses.Results:The GI value of wheat flour food, oat fiber food, RS food was 88.24?20.84,60.16?14.16,47.05?10.22 respectively,regarding glucose powder as 100 . The II value of them was 83.06 ? 10.68 , 68.32 ? 17.08, 60.26 ? 30.1 respectively.The area and peak level under the glucose-response curve and insulin-response curve of RS food were significantly lower than those of glucose powder and wheat flour food (P

Objective: To examine the effects of RS on serum cholesterol and expression of genes associated with cholesterol metabolism in rat liver for understanding its mechanism. Method: Eighteen rats were randomly assigned to three groups, six rats each. They were fed with chemical purified normal diet (control group), low RS (15%Hi-maize 1043) diet and high RS (30%Hi-maize 1043) diet respectively. Six weeks later, serum cholesterol and short chain fatty acid (SCFA) in the cecum were measured. Likewise, hepatic cholesterol 7?-hydroxylase, LDL receptor, and SR-B1 mRNA levels were measured by realtime PCR. Results: Hepatic cholesterol 7a-hydroxylase, LDL receptor, SR-B1 mRNA levels and cecal acetate, propionate and n-butyrate concentrations in the high RS group were significantly higher than those in the control group (P

Firstly, the essay introduces the definition of bio-burden, the significance of determination of bio-burden and the specific performance requirements. Then, the essay introduces the method selection and validation on the bio-burden determination of surgical drapes, gowns and clean air suits.
Equipment Contamination ; Surgical Attire ; microbiology ; standards ; Surgical Drapes ; microbiology ; standards

BACKGROUND:Knee osteoarthritis and rheumatoid arthritis are common arthritis in the knee.In late stage of arthritis,severe deformation and activity obstacle are found,which require total knee replacement.The total knee replacement not only can significantly relieve symptom,but also correct deformation and improve joint function.OBJECTIVE:To summarize classification of knee joint,techniques of knee replacement surgery and complications following replacement.METHODS:We retrieved Vip Database (http://www.cqvip.com) for titles and abstracts with the key words of "knee replacement,prosthesis,complication".Articles concerning clinical application of knee prosthesis were included.Articles in the same field that published recently or in authorized journals were included.A total of 20 literatures were included for review according to inclusion criteria.RESULTS AND CONCLUSION:Various artificial total knee prostheses were used in different patients in accordance with patients' condition,joint limitation degree,fixation method and meniscus function.Indication of different knee prostheses depends on bone and soft tissue of the knee,and quality and status of knee ligament,joint deformation and destruction degree of articular cartilage are responsible for the choice of prosthesis.Thrombosis of deep veins of the lower limb,pulmonary embolism,infection surrounding the prosthesis and prosthesis loose are common complications in joint replacement.

[Objective]To investigate the therapeutic effect of traditional Chinese herb and selective medial vastus muscle electric stimulation in treatment of chondromalacia of patella(CMP).[Method]Ninety CMP cases were selected and divided into 3 treatment groups,with 30 cases in each one.Traditional Chinese herb was applied in group A.Selected medial vastus muscle electric stimulation was applied in group B.Combination of both treatments was applied in group C.All cases were followed up for 9-12 months.Congruence angle(CA) and lateral patello-femoral angle(LPA) were measured and statistical analysis was used to investigate the therapeutic effect.[Result]CA and LPA showed significant differences between pre-and postoperative results(P0.05).[Conclusion]Traditional Chinese herb combined with selective medial vastus muscle electric stimulation is a effective therapy in treatment of chondromalacia of patella.