OBJECTIVE:To establish a quality standard for Shenning tablet.METHODS:Leonurus japonicus,Radix et Rhizoma Rhei,Gardenia jasminoides,Phellodendron chinense in Shenning tablet was identified by TLC.The contents of Jasminoidin and Peoniflorin in Shenning tablet were determined by HPLC.RESULTS:The relevant spots in Leonurus japonicus,Radix et Rhizoma Rhei,Gardenia jasminoides,Phellodendron chinense were identified by TLC.The linear ranges of Jasminoidin and Peoniflorin were 0.032 2～0.386 4 ?g(r=0.999 9) and 0.041～0.492 ?g(r=0.999 9),respecctively,with average recovery rates at 101.09% and 100.08%,respectively and RSD at 0.54% and 1.28%,respectively(n=6).CONCLUSION:The established quality standard is suitable for the quality control of Shenning tablet.

Objective To observe the effects of resistant starch (RS) on insulin resistance (IR) in type 2 diabetes mellitus rats. Method Male Wistar rats (n=40) were used.Ten were fed with normal diets (normal control group) and the others with high fat (safflower oil, 59% of total energy) diet (model group) . Ten weeks laters, the model group was injected with streptozotocin (20mg/kg bw) and divided into 3 groups: diabetes control group, high fat and RS intervention group, RS intervention group. After 5 w, oral glucose tolerance test (OGTT) was carried out in each group and the metabolism of glucose and lipid, insulin sensitivity, pathological changes of liver and kidney were observed. Results Compared with diabetes control group, insulin sensitivity index (ISI) was higher and area under curve (AUC), fasting insulin (FINS), total cholesterol (TC), triglyceride (TG), low density-lipoprotein cholesterol (LDL-C) were significantly lower in RS intervention groups. Besides, the fatty degeneration of liver was much lighter but glycogen synthesis was significantly increased. Conclusion RS is effective in improving IR of type 2 diabetes mellitus rats.

Cancer molecular epidemiology focus on distribution and pathogenesis of tumor markers in high-risk populations and cancer patients.Cancer molecular epidemiology tries to open the "black box" and to elucidate the nature of cancer through the measurement and evaluation of exposure and response to carcinogens and individual genetic susceptibility,involving the fields of etiology,preventive medicine and clinical medicine.We need rigorously strengthen the design and implementation of cancer molecular epidemiology,the research and application of TM should enhance the principles of quality,evidence and ethics.With the utility of new subject tools such as proteomics,cancer molecular epidemiology which combines the advantages of multi-subjects is now quickly changing.

Objective To explore the relationship between polymorphism of angiotensin converting enzyme (ACE) gene and vascular dementia (VD).Methods 94 cases of VD, 60 cases of essential hypertension (EH) and 60 healthy controls were enrolled to analyze insertion/deletion polymorphism of ACE gene by polymerase chain reaction (PCR).Results The DD genotype and the frequency of D allele were significantly higher in VD patients than in healthy controls (all P

BACKGROUND:An increasing number of studies have shown that mesenchymal stem cels have the potential to treat acute kidney injury. Umbilical cord-derived mesenchymal stem cels have general characteristics of stem cels and many advantages, such as easy to isolate and culture, in vitrofast amplification, low immunogenicity and no ethical problems, which have garnered increasing attentions. OBJECTIVE:To study the repairing effects of human umbilical cord-derived mesenchymal stem cels on acute kidney injury in rats. METHODS: Thirty rats were randomized into three groups: a normal control group, a model group and a cel transplantation group. Rats in the model and cel transplantation were subjected to clamping the renal pedicles for 45 minutes, and then injected 1 mL of DAPI-labeled umbilical cord-derived mesenchymal stem cels or 1 mL of saline via the tail vein. In the normal control group, the kidney was only exposed with no treatment. At 7 days after treatment, the rats were kiled to take left kidney tissues for pathological observation under light microscope and right kidney for observation of DAPI-positive cel counting. Automatic biochemical analyzer was used to detect serum creatinine and urea ammonia levels. RESULTS AND CONCLUSION: Compared with the model group, the levels of serum creatinine and urea ammonia were significantly lower in the cel transplantation group (P < 0.05), suggesting that human umbilical cord-derived mesenchymal stem cels can improve the kidney function to a certain extent. Pathological findings showed that the pathological damage was improved more remarkably in the cel transplantation group than the model group, and the tubular necrosis index decreased significantly in the cel transplantation group. At 7 days after cel transplantation, blue fluorescent cels were scattered on renal tissue frozen sections. These results indicate that human umbilical cord-derived mesenchymal stem cels can migrate to the injured tubular epithelial tissues, and promote the repair of the injured kidney.

Objective:The glucemic index(GI)and insulin index(II)of wheat flour, oat fiber and resistant starch (RS) were studied.Method:Ten healthy subjects consumed 4 kinds of carbohydrate foods: glucose powder,wheat flour food, oat fiber food and RS food.Up to 120 min after consumption of test materials, blood samples were taken for glucose and insulin analyses.Results:The GI value of wheat flour food, oat fiber food, RS food was 88.24?20.84,60.16?14.16,47.05?10.22 respectively,regarding glucose powder as 100 . The II value of them was 83.06 ? 10.68 , 68.32 ? 17.08, 60.26 ? 30.1 respectively.The area and peak level under the glucose-response curve and insulin-response curve of RS food were significantly lower than those of glucose powder and wheat flour food (P

Objective: To examine the effects of RS on serum cholesterol and expression of genes associated with cholesterol metabolism in rat liver for understanding its mechanism. Method: Eighteen rats were randomly assigned to three groups, six rats each. They were fed with chemical purified normal diet (control group), low RS (15%Hi-maize 1043) diet and high RS (30%Hi-maize 1043) diet respectively. Six weeks later, serum cholesterol and short chain fatty acid (SCFA) in the cecum were measured. Likewise, hepatic cholesterol 7?-hydroxylase, LDL receptor, and SR-B1 mRNA levels were measured by realtime PCR. Results: Hepatic cholesterol 7a-hydroxylase, LDL receptor, SR-B1 mRNA levels and cecal acetate, propionate and n-butyrate concentrations in the high RS group were significantly higher than those in the control group (P

0\^05). The negative rate of DIGFA in healthy people was 100%(40/40). The cross reaction rate in 20 cysticercosis cases and 25 schistosomiasis cases were 5%(1/20) and 4%(1/25), respectively. Both coincidence rates comparing DIGFA with dot\|ELISA were 90\^9%(50/55). Conclusion DIGFA is as sensitive and specific as the dot\|ELISA,and has the advantages of simplicity and without specific equipment.

Objective: To study the defense style of undergraduates with cluster B personality disorders. Methods:26 undergraduates with cluster B personality disorders were diagnosed by PDQ~ +4 and PDI from 2826 undergraduates, then they and 63 normal undergraduates were assessed with DSQ (defensive style questionnaire). Results: ①The incidence of histrionic, narcissistic, borderline and antisocial personality disorders were 0.25%?0.50%?0.39% and 0.11% .②The undergraduates with B cluster personality disorder had higher scores in immature defenses and idealization, omnipotenic-devaluation and isolation than normal control (t=3.18-7.03)(P

Objective: To construct the recombinant expressive vector of HSV-2-EGFP for the primary application in rapid,direct and sensitive diagnosis of herpes simplex virus infection. Methods: The gene fragment coding for HSV-2 ICP10 promoter was amplified by PCR,and the PCR product was cloned into pEGFP-1 to construct the recombinant plasmid pICP10-EGFP,which would be identified by DNA sequencing.The recombinant plasmid pICP10-EGFP were transfected into Vero cells by cation lipoid Lipofectamine 2000,then G418 was added to screen the positive cells to obtain stable cell line Vero-ICP10-EGFP.Vero-ICP10-EGFP was infected by various titers of HSV-2,and the EGFP fluorescence was detected under inverted microscope at 6,8 and 10 h post-infection.The specificity of the cell line was detected by infection with human cytomegalovirus(HCMV) and coxsackievirus. Results: The recombinant plasmid pICP10-EGFP was proved to be correct by the double restriction enzyme digestion and DNA sequencing.The Vero-ICP10-EGFP fluorescent emitting cells can be observed as early as 6 h after infection with HSV,with pronounced increase in the intensities at later hours.No induction of detectable fluorescence was detected by infection with HCMV and coxsackievirus 6,10 and 24 h post-infection. Conclusion: This novel GFP reporter system expressing the HSV-inducible EGFP reporter gene might provide a fast,easy and sensitive model for monitoring HSV in clinical specimens.