Objective To explore the mechanism of imperfect recovery of the renal function after release of chronic ureteral obstruction. Methods Adult New Zealand rabbits(n=36) were randomly divided into normal control group(n=3),obstructive control group(n=3) and experimental group(n=30).The latter two groups were subjected to chronic unilateral ureteral obstruction.Two weeks later,3 rabbits were sacrificed and the others underwent ureterostomy,which were sacrificed separately following 12 hours,24 hours,3 days,7 days,14 days.The renal papilla and cortex were examined by scanning electron microscopy. Results Shortly after the release of ureteral obstruction,massive loss of brush border and ballooning of epithelial cells were noted in proximal tubules.Distal tubules and collecting tubules also showed severe ballooning of epithelial cells and normal struction were not seen.Two weeks later,many tubular components became patent with normal epithelial lining.Some of them,however,were deteriorated further in spite of the release of obstruction.Thus there was a marked tubular heterogeneity in recovery.Conclusion Further deterioration of some tubules might be one of the important pathologic basis for imperfect recovery of renal function after the release of chronic ureteral obstruction.

Objective To study the effect of tamoxifen on the proliferation and apoptosis of germ cells and to evaluate its clinical application to idiopathic oligospermia. Methods 41 oligospermia patients were treated with tamoxifen.Testicular tissues obtained by Tur Cut biopsy needle,both before and after the treatment,were observed under electron microscopy,the incidence of apoptosis and PCNA expression in germ cells being detected. Results Testicular pathological changes in different degree were observed on electron microscopy.Compared with the normal controls.PCNA proliferation index (PI) was lower ( P

Objective　To study the occurence, development and regulation of reactive gliosis with astrocyte (Ast) in vitro. Methods　Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis. Results　After scratching, the astrocytes showed typical features of reactive gliosis, with the hypertrophic cell body, thickened and lengtheded processes, and enhanced glial fibrillary acidic protein (GFAP) staining. In situ hybridization and RT-PCR analysis confirmed that the expression of GFAP mRNA was markedly increased. These changes occurred 1 d after scratching and reached the peak 5 to 7 d after injuring. Conclusion　A model of reactive astrogliosis was successfully established in vitro which showed an active reaction to injury. The characteristics of reactive gliosis parallel that seen in vivo.

The changes of the morphology and percentage of the macroglia surrounding focus of brain stabbing injury (BSI) were observed by immunohistochemistry,immunoinfluorescence stain and flow cytometer(FCM) and the effect of magroglia during glial scar forming were elucidated.The results showed that a large number of GFAP-immunoreactive positive cells were accumulated around the focus of BSI.These cells were hyperplastic,hypertrophic,and emerged swollen cytoplasmic processes.The most marked changes were observed at 1-2 week after BSI.The results of FCM showed that the percentage of GFAP positive cells increased gradually and reached to a peak during 1～2 week after BSI.The peak ratio of GFAP positive was about 46%.However,the changes of morphology and number of GC positive cells were not detected after BSI.The authors believed that astrocyte is the main macroglia during glial scar formatting .The oligodendrocytes is not an active cell during this course.

Objective To investigate the therapeutic effects of traditional Chinese medicinal herbs(TCMH) on benign prostate hyperplasia(BPH) and their influence on proliferation and apoptosis of prostate cells in experimental mice. Methods A total of 120 male Kunming mice(weight,30 to 40 g)were randomly divided into 5 groups:①normal control group;②hyperplastic group;③normal saline(NS)(negative control)group;④TCMH group;⑤finasteride (positive control) group.Each group included 24 mice.The mice in normal control group were killed and their prostates were weighed.The BPH model was induced by implanting the urogenital sinus in the mice in other 4 groups.In the 61st day,the prostate weight of the mice in hyperplastic group was examined after they were killed;and the mice in the remaining groups were fed with NS,TCMH and finasteride,respectively.In the 91st day,the mice in these 3 groups were killed and their prostates were weighed.The proliferation and apoptosis of the prostate cells of mice in the 3 groups were determined by flow cytometry(FCM). Results The prostates in hyperplastic group [(149.30?8.46)mg] were heavier than those in TCMH group [(85.60?17.97)mg] ( P 0.05). Conclusions The TCMH has a significant therapeutic effects on experimental mice with BPH.The mechanisms may be as follows:TCMH can regulate the relevant genes of the prostate cells so that they can promote the apoptosis of the prostate cells and as a result,the volume and weight of the prostate of the mice are reduced.

Objective To investigate the selective killing effect of adenoviral mediated herpes simplex virus-thymidine kinase/ganciclovir (HSV-tk/GCV) suicide gene system controlled by human telomerase reverse transcriptase (hTERT) promoter on bladder cancer cells in vitro. Methods Bladder cancer cell line 253J and human fibroblast cell line MRC-5 were transfected by the recombinant adenovirus of different multiplicities of infection (MOI),and the infection rate was measured by observing the expression of enhanced green fluorescent protein (EGFP) under the fluorescent microscopy.Ad-hTERT-HSV/tk and Ad-CMV-HSV/tk were transduced into 253J and MRC-5,followed by GCV treatment. The relative survival rate of cells in presence of prodrug GCV was measured with MTT method. Results Recombinant adenovirus Ad-hTERT-EGFP could selectively infect 253J cells,with the infection rate associated with the increasing MOI of recombinant adenovirus (P

Objective To explore the effects of ureteropelvic junction(UPJ) obstruction on contralateral kidney.Methods Twenty-four adult New Zealand rabbits were divided randomly into 3 groups,ie,complete obstruction group(group 1,n=8),partial obstruction group(group 2,n=8) and shamoperation group(group 3,n=8).In groups 1 and 2, the right ureter was ligated completely or partially.In group 3,the right ureter was only exposed but not ligated.The animals were killed 4 weeks after operation,and the contralateral kidneys were resected.Before killing,anterior urography showed that right hydronephrosis occurred in all the rabbits in groups 1 and 2.The contralateral renal weight was measured by electron balance.The apoptosis in the contralateral renal cortex and medulla was detected by FITC-AnnexinV/PI flow cytometric assay.The morphological changes of the samples were also studied.Results After 4 weeks,the contralateral renal weight of the rabbits in groups 1,2 and 3 was(3.96?0.20),(3.47?0.19) and(3.20?0.21)g/kgBW,respectively.The difference of contralateral renal weight between groups 1,2 and group 3 was significant(P0.05).In contrast,the apoptosis rates of contralateral renal medulla cells in groups 1,2 and 3 were(23.75?2.34)%,(11.82?1.40)% and(2.36?0.65)%,respectively.There were significant differences between these 3 groups(P

Objective To study the ultrastructural changes in three different zones of benign prostatic hyperplasia(BPH). Methods The ultrastructural changes in peripheral,transitional and central zones(PZ,TZ and CZ)of prostates from 10 patients with BPH were observed with transmission electron microscopy. Results Glandular epithelia layers in TZ of BPH were increased.The stroma in TZ was mainly composed of smooth muscle cells (SMC) of vigorous proliferation and fibroblasts.Mast cell and lymphocyte with cytoplasma membrane merge were observed in this zone.In PZ and CZ of BPH, SMC showing degenerative changes,such as,nuclear pyknosis,dilatation of perinuclear space and vacuolation of cytoplasma were frequently seen. Conclusions There are significant differences in the ultrastructure of three zones in BPH.

ObjectiveTo observe the expression of vascular endothlial growth factor(VEGF) and throbospondin-1(TSP-1) in different grade bladder cancer.MethodsSpecimens of 70 cases of bladder transitional cell cancer including Grade Ⅰ 28 cases,Grade Ⅱ 27 cases,Grade Ⅲ 15 cases(according to WHO pathological grade) were stained with immunohistochemistry method.The expressing values of VEGF and TSP-1 were calculated by mean vascular density(MVD).All data was analyzed with SPSS 10.0,and with expression of VEGF and TSP-1 was detected with immunohistochemisrty double staining.Finally,the result of expression was observed by laser scan with focus microscope.ResultsThere was a positive correlation between the cancer grade and the expression of VEGF,and the expression of Grade Ⅰ was significant difference with that of Grade Ⅱ(P<0.01),but there was no difference between Grade Ⅱ and Grade Ⅲ(P>0.1).Expression of TSP-1 decreased with grade increasing but not showing a negative correlation,while,Grade Ⅰ was significant difference with that of Grade Ⅱ and Grade Ⅲ(P<0.01),Grade Ⅱ was not differcnt with Grade Ⅲ(P>0.1).In 28 Grade Ⅰ cases,values of MVD of VEGF and TSP-1 had no correlation(rs)=0.167,P>0.1).Double immunostaining detecting with laser scan with focus microcope indicate showed that VEGF and TSP-1 appeared in same bladder cancer specimen.ConclusionExpression of VEGF is gradually increased from Grade Ⅰ to Grade Ⅲ,and VEGF is a promoting factor of angiogenesic bladder cancer.Expression of TSP-1 is the strongest in Grade Ⅰ cases,and TSP-1 is an inhibiting angiogenesic factor,which inhibiting function only in early stage.Immunofluorescent staining can only provide evidence of together expression of VEGF and TSP-1,cannot evaluate the degree of expression.

BACKGROUND: There are disputes concerning limb salvage and reconstruction in treating pelvis malignant tumor.OBJECTIVE: To study the surgical treatment following resection of tumor at acetabular region in order to restore pelvic stability with less damage or recurrence.METHODS: Thirty-three patients diagnosed with pelvic tumor at Department of Orthopaedics, First Affiliated Hospital of Second Military Medical University from April 2002 to June 2008 were selected. All patients were received tumor resection and computer-aided prosthetic replacement, 18 patients (8 cases with chondrosarcoma, 2 cases with Ewing sarcoma, 2 cases with osteosarcoma, and 6 cases with malignant fibrous histiocytoma) were combined with antitumor drug treatment. Functional assessment of therapeutic efficacy was divided into 4 levels: excellent, good, moderate, and inferior.RESULTS AND CONCLUSION: Two patients died at months 4 and 6 after prosthetic replacement; 31 cases were followed-up for 8-32 months, mean 12.5 months. Seven cases were recurred chondrosarcoma, the recovery of other patients were as follow: 18 cases were excellent, 4 cases were good, 2 cases were moderate and no case was inferior. Most of limb function and pelvic stability was preserved. The results suggested that malignant fibrous histiocytoma and Ewing sarcoma can receive a high rate of limb preservation if treated by effective chemotherapy with low recurrence; the recurrence of chondrosarcoma was high. Tumor resection combined with computer-aided prosthetic replacement can maximatily reserve limb function and raise life quality.