Objective ToobservetheeffectsofNaokangⅡ,atraditionalChinesemedicineon spatial learning and memory abilities and hippocampal neurogenesis in rats with cerebral ischemia. Methods Atotalof54SDratsweredividedinto6groupsaccordingtothedigitalrandommethod:a control,a sham operation,a model,a low-dose,a medium-dose,and a high-dose NaokangⅡ group (the low-,medium-,and high-dose groups were 5,10,and 20 times of adults (n=9 in each group). A rat model was induced by ligate the bilateral common carotid arteries permanently. The bilateral common carotid arteries were only isolated but not ligated in rats of the sham operation group. The rats in the control group were not given any treatment. After modeling,the rats in the treatment groups were treated with NaokangⅡ. The rats in the control,sham operation,and model groups were given equal volume of isotonic saline (2 ml). Morris water maze test and space search experiment were used to evaluate the learning and memory abilities in rats 9 weeks after modeling. Immunofluorescence staining and immunohistochemical methods were used to observe proliferation and differentiation of neural stem cells (NSCs). Western blot was used to detect the expression of autophagy related protein Beclin-1 and LC-3Ⅱ. Results (1 )The escape latencies of the model group from 1 to 5 days were 100 ± 13,98 ± 13,82 ± 14,72 ± 15,and 46 ± 7 s,respectively. They were significantly longer than those of the sham operation group and the control group (all P<0. 05). The search distances were 1239 ± 127,1011 ± 122,959 ± 123,553 ± 66,and 407 ± 29 cm,respectively. They were significantly increased as compared with those of the sham operation group and control group (all P<0. 01). Both the escape latencies and search distances of the rats in the Naokang Ⅱ low-,medium-,and high-dose groups were significantly shorter than those of the model group (P<0. 05). The escape latencies on the fifth day were 20 ± 6,19 ± 8,and 15 ± 6 s,respectively,and the search distances were 234 ± 38, 297 ± 23,and 99 ± 22 cm,respectively. Nine weeks after modeling,the quadrant search time on the original platform in the rats of the model group was 30 ± 9 s,it was significantly shorter than that of the sham operation group (P<0. 01). The quadrant search time on the original platform of the Naokang Ⅱ low-, medium-,and high-dose groups were 34 ± 6,38 ± 8,and 40 ± 10 s,respectively. They were significantly longer than those of the model group (P <0. 01). (2)Nine weeks after modeling,the results of immunofluorescence and immunohistochemistry showed that the number of BrdU-positive cells and the Nestin positive cells in hippocampal dentate gyrus of the model group were higher than those of the control group and the sham operation group (P<0. 05). The number of BrdU-positive cells and Nestin positive cells of Naokang Ⅱ each dose group were significantly higher than those of the model group (P<0. 05). The expression of LC-3Ⅱand Beclin-1 in the model group was significantly higher than those in the control and sham operation groups (P<0. 01). The expression of LC-3Ⅱ in the medium- and high-dose groups was 143 ± 9 and 129 ± 9 respectively,and the expression of Beclin-1 was 139 ± 10 and 124 ± 7 respectively, andtheyweresignificantlylowerthanthemodelgroup(P<0.01).Conclusion NaokangⅡcanimprove the cognitive function in rats with cerebral ischemia,promote neurogenesis in the dentate gyrus,and inhibit autophagic cell death.

0.05 ).However,there were more eccentric plaques in ruptured than in non-ruptured groups ( P

Objective To explore the value of intravascular ultrasound (IVUS) in diagnosing plaque disruption and arterial thrombosis.Methods Balloon-induced arterial wall injury and then feeding on a diet of 1% cholesterol for 8 weeks, 17 New Zealand white rabbits were established as atherosis(AS) models.At the end of the eighth week, the resulting plaques were incubated transluminally with recombinant adenovirus carrying a p53 transgene. After two weeks, the rabbits underwent pharmacological triggering with Russell viper venom(RVV) and histamine. IVUS was used before and after the rabbits were triggered pharmacologically. The value of IVUS in diagnosing plaque disruption and arterial thrombosis was derived by comparing the results of IVUS with pathologic findings. Results IVUS found that plaques disruption and thrombosis occurred in 12 of the 14 rabbits, totally 18 thrombi. A histologically confirmed plaque disruption and intraluminal thrombus were visualized in 12 of the 14 rabbits, totally 20 thrombi. By comparing the results of IVUS with histopathology, the true positive rate was 85% and false negative rate was 15%, the sensitivity and specificity of IVUS were respectively 85% and 67%. IVUS data correlated well with the histopathology regarding thrombus length(r=(0.73),P(0.05)). Conclusions IVUS can detect plaque disruption and arterial thrombosis.

Objective To study the intervention of Daotan Decoction to the expression of ICAM-1 in cerebrovascular endothelial cells. Methods Cerebrovascular endothelial cells were cultured to observed the influence of ICAM-1 expression,which was induced by TNF-?,by the way of immunocytochemistry methods. Results Compared with control group,the expression of ICAM-1 in model group was time-dependent (P

Objective To analyse the TCM syndrome of chronic fatigue syndrome (CFS) patients by discriminative analysis. Methods 237 CFS patients were collected to made a stepwise discriminative analysis of symptom variables, basing on their TCM syndromes. Results 10 variables were obtained from symptoms by the way of stepwise discriminative analysis (P

Objective To analyse the relationship of blood deficiency syndrome’s principal components of chronic fatigue syndrome (CFS) and mental state and quality of life. Methods 23 cases of CFS blood deficiency syndrome were collected to pick up principal components, and combined with mental state (SCL-90) and quality of life (SF-36). Then, the relationship was analyzed. Results There was significant relationship between CFS blood syndrome principal component 1 and GH, VT and BP in SF-36. And there was significant relationship between CFS blood syndrome principal component 2 and patients’ somatization, obsessive compulsive, interpersonal sensitive, depression, anxiety, hostility and paranoid in SCL-90, and between principal component 1 and somatization. Conclusion There were two principal components in CFS blood deficiency syndrome, one influenced patients’ quality of life, the other influenced their mental state.

objective To analyse the traditional Chinese medicine syndromes of lung cancer by the way of variable cluster analysis. Methods 176 cases of lung cancer were collected and their symptoms were recorded. Then these patients’ clinical data were analyzed by variable cluster analysis. Results The Chinese medicine syndromes of these patients could be divided into 5 types, and the variable proportion is 50.94%. Conclusion TCM syndromes of metaphase and advanced lung cancer patients include both deficiency and excessive syndrome, mainly deficiency syndrome with. And, the way of variable cluster analysis could help TCM syndrome differentiation of lung cancer patients.

Humans ; Myocardial Ischemia ; etiology

Objective To investigate the distribution and antibiotic resistance patterns of uropathogens in children with complicated urinary tract infection to guide the empirical antimicrobial treatment.Methods Retrospective analysis of isolated bacteria from 141 children hospitalized with complicated urinary tract infection was performed from January 2011 to January 2013.Results Gram-negative bacillus were the main pathogens [56.6％(81/143)],and Escherichia coli was the leading cause (41.3％,59/143).Gram-positive bacillus accounted for 38.5％ (55/143),including Enterococcus faecium (17.5％,25/143) and Enterococcus faecalis (14.7％,21/143).Fungi caused 4.9％ (7/143) of the infections.The resistance of Escherichia coli to amoxicillin was the highest [92.0％ (23/25)] and resistance to imipenem (4.3％,2/47),amikacin (6.8％,4/59) and nitrofurantoin (6.8％,4/59) was lower.It was observed resistance of Enterococcus faecium to linezolid,quinupristin,vancomycin and nitrofurantoin was significantly lower than other common antibiotics.The resistance of Enterococcus faecalis to quinupristin,tetracycline,erythromycin,rifampicin was significantly higher than other common antibiotics.Concluusions Escherichia coli,Enterococcus faecium and Enterococcus faecalis are the common uropathogens.These pathogens have a high antibiotic resistance to many common antibiotics.The selection of empirical treatment of children with complicated urinary tract infection should be adjusted by antimicrobial susceptibility test.

Objective To explore the relationship of professional stress,social support and somatic symptoms of sub-health status of Ningbo normal senior school teachers. Methods 128 normal senior school teachers were selected and investigated by professional stress scale,social support scale and somatic symptoms of sub-health status scale, and canonical correlation analysis was used. Results The canonical correlation coefficient were 0.563, 0.471( P <0.01) with the existence of social support variable. The somatic symptoms of sub-health status would be greater with the rise of their professional stress and the decline of their social support. The canonical correlation coefficient was 0.497( P <0.01) with the elimination of social support variable. The factor loading of "examination pressure","professional burden" and "career expectation" in canonical variable ξ add to 0.801,0.855,0.726 respectively. The factor loading of "feeling of fatigue","sleep disorders" in canonical variable η add to 0.774,0.859 respectively. Conclusion The social support have adjustment function between the professional stress and somatic symptoms of sub-health status.